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The map label for this gene is tcyA [H]

Identifier: 77459080

GI number: 77459080

Start: 3293283

End: 3294107

Strand: Direct

Name: tcyA [H]

Synonym: Pfl01_2855

Alternate gene names: 77459080

Gene position: 3293283-3294107 (Clockwise)

Preceding gene: 77459079

Following gene: 77459081

Centisome position: 51.15

GC content: 59.64

Gene sequence:

>825_bases
ATGAAGAACTTCGTCATTCCAGCAGTACTCACCTCCCTGATGTCTTGCGGTGTCGCCGTGGCCGCCGAATTGCCGGCCAG
CATCAAGGAAAAAGGCGAGATCGTCGTCGCGATCATGCCCAACTATCCGCCGATGGACTTCAAGGACCCGGCCACCAACA
AGCTCACCGGCCTTGACTACGACCTGGGCAACGCCCTGGCCGAACGCCTCGGGGTGAAGATCAAGTGGCAGGAAACCGGC
TTCGAGCAAATGATCAACGCCCTGACCACCGAGCGCGTGGACATGGTTCTGTCGGGCATGACCGACACCGCCGAGCGCCA
GGCCAGCGTGACCTTCGTCGACTACTTCACCAGCGGCCCGCAGTTCTACACCTTGCAGAAGAACGCAACGACCAATGAAA
TCATCGACCTGTGCGGCAAGAAAGTCGGCACCAGCCGCCGCACCACCTTCCCGGCGGAAATCGCTGCGTGGAGCAAGGAA
AACTGTGAAGCGGCCGGCAAACCTGCAATCAACGTGATCGGCACCGAAGGCTCGGCCGACGCCCGTGCGCAACTGCGCCA
GAGCCGCATCGATGCGGCGATGCAGGGCAGCGAAACCCTGTCGTACCTCAAGACTCAGGAAAAGGACATGTACAAGACGG
TCGGCCAGCCGATCTCCGTGCAGTTCACCGGGTTGGGTGTGAGCAAGAAGAAGCCGGAGCTGAGTGAAGCGGTGAAGGTG
GCGTTGCAGAGCATGGTGGATGACGGCAGCTATAACGCGATTCTGAAGAAATGGGATCTGGAGCTGGGTGCGATCAAGGA
TGTGACCATTAACGCGGGCAAGTAA

Upstream 100 bases:

>100_bases
AATACTAATAAGTCCGCAAAACCCGCGCACCCAAAGCTTCATTTCTGCCGTTCGAACCTGAGGCCCCTTTGGCCTTCACA
ACACTCATAAGAGAACGTCC

Downstream 100 bases:

>100_bases
ACCGGTCAGCGCCGACCCAACCGGTCGGCGCCTTTTTCATGAACAGCCGGAGCGCCCTTGATGTCCTCCTTGCAACCCAC
CTGGCCCGACCAGCACAAAG

Product: extracellular solute-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 274; Mature: 274

Protein sequence:

>274_residues
MKNFVIPAVLTSLMSCGVAVAAELPASIKEKGEIVVAIMPNYPPMDFKDPATNKLTGLDYDLGNALAERLGVKIKWQETG
FEQMINALTTERVDMVLSGMTDTAERQASVTFVDYFTSGPQFYTLQKNATTNEIIDLCGKKVGTSRRTTFPAEIAAWSKE
NCEAAGKPAINVIGTEGSADARAQLRQSRIDAAMQGSETLSYLKTQEKDMYKTVGQPISVQFTGLGVSKKKPELSEAVKV
ALQSMVDDGSYNAILKKWDLELGAIKDVTINAGK

Sequences:

>Translated_274_residues
MKNFVIPAVLTSLMSCGVAVAAELPASIKEKGEIVVAIMPNYPPMDFKDPATNKLTGLDYDLGNALAERLGVKIKWQETG
FEQMINALTTERVDMVLSGMTDTAERQASVTFVDYFTSGPQFYTLQKNATTNEIIDLCGKKVGTSRRTTFPAEIAAWSKE
NCEAAGKPAINVIGTEGSADARAQLRQSRIDAAMQGSETLSYLKTQEKDMYKTVGQPISVQFTGLGVSKKKPELSEAVKV
ALQSMVDDGSYNAILKKWDLELGAIKDVTINAGK
>Mature_274_residues
MKNFVIPAVLTSLMSCGVAVAAELPASIKEKGEIVVAIMPNYPPMDFKDPATNKLTGLDYDLGNALAERLGVKIKWQETG
FEQMINALTTERVDMVLSGMTDTAERQASVTFVDYFTSGPQFYTLQKNATTNEIIDLCGKKVGTSRRTTFPAEIAAWSKE
NCEAAGKPAINVIGTEGSADARAQLRQSRIDAAMQGSETLSYLKTQEKDMYKTVGQPISVQFTGLGVSKKKPELSEAVKV
ALQSMVDDGSYNAILKKWDLELGAIKDVTINAGK

Specific function: Part of the ABC transporter complex tcyABC involved in L-cystine import [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788228, Length=256, Percent_Identity=28.125, Blast_Score=91, Evalue=6e-20,
Organism=Escherichia coli, GI1787031, Length=249, Percent_Identity=29.718875502008, Blast_Score=91, Evalue=6e-20,
Organism=Escherichia coli, GI1788649, Length=266, Percent_Identity=25.5639097744361, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1788648, Length=233, Percent_Identity=27.0386266094421, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1787088, Length=256, Percent_Identity=26.5625, Blast_Score=81, Evalue=8e-17,
Organism=Escherichia coli, GI1787085, Length=266, Percent_Identity=27.0676691729323, Blast_Score=71, Evalue=9e-14,

Paralogues:

None

Copy number: 1920 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1060 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 80 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 29703; Mature: 29703

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNFVIPAVLTSLMSCGVAVAAELPASIKEKGEIVVAIMPNYPPMDFKDPATNKLTGLDY
CCCEEHHHHHHHHHHCCHHHHHHCCHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCCC
DLGNALAERLGVKIKWQETGFEQMINALTTERVDMVLSGMTDTAERQASVTFVDYFTSGP
HHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHCEEEEEEEECCCC
QFYTLQKNATTNEIIDLCGKKVGTSRRTTFPAEIAAWSKENCEAAGKPAINVIGTEGSAD
EEEEEECCCCHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCCCCCCCCCCEEEEECCCCHH
ARAQLRQSRIDAAMQGSETLSYLKTQEKDMYKTVGQPISVQFTGLGVSKKKPELSEAVKV
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHH
ALQSMVDDGSYNAILKKWDLELGAIKDVTINAGK
HHHHHHCCCCHHHHHHHHCCCCCCEEEEEEECCC
>Mature Secondary Structure
MKNFVIPAVLTSLMSCGVAVAAELPASIKEKGEIVVAIMPNYPPMDFKDPATNKLTGLDY
CCCEEHHHHHHHHHHCCHHHHHHCCHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCCC
DLGNALAERLGVKIKWQETGFEQMINALTTERVDMVLSGMTDTAERQASVTFVDYFTSGP
HHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHCEEEEEEEECCCC
QFYTLQKNATTNEIIDLCGKKVGTSRRTTFPAEIAAWSKENCEAAGKPAINVIGTEGSAD
EEEEEECCCCHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCCCCCCCCCCEEEEECCCCHH
ARAQLRQSRIDAAMQGSETLSYLKTQEKDMYKTVGQPISVQFTGLGVSKKKPELSEAVKV
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHH
ALQSMVDDGSYNAILKKWDLELGAIKDVTINAGK
HHHHHHCCCCHHHHHHHHCCCCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7551042; 8969502; 9384377 [H]