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The map label for this gene is ycdJ [C]

Identifier: 77458642

GI number: 77458642

Start: 2773194

End: 2774102

Strand: Reverse

Name: ycdJ [C]

Synonym: Pfl01_2417

Alternate gene names: 77458642

Gene position: 2774102-2773194 (Counterclockwise)

Preceding gene: 77458643

Following gene: 77458641

Centisome position: 43.09

GC content: 64.69

Gene sequence:

>909_bases
ATGATCCGCGCCCTGCTCACCGCTTCCCTGCTGCTGGCCATGAGTGTTCCGGCCCATGCGGATGTGACGCCCTTCCCCGC
CTCGTTCCGCACTCAGGACATCGCCGTGGACGGCGCGAAAATCCACACGCGCATCGGCGGAAAAGGCCCGGCGGTGGTGC
TGTTGCACGGTTTCGGCGACACCGGTGATATGTGGGCGCCGCTGGCGGCGGATCTGGCGAAGGATCACACCGTGGTGGTG
CCTGATTTGCGGGGCATGGGCCTGTCGTCGATCCCGGCCGATGGCTACGACAAGAAGACCCAGGCCGGTGATATCCGCGC
GGTGCTGGCTTCGCTGGGCATCGAGCATTCGGTGGTGGTCGGCCACGACATCGGCACCATGGTCGCCTTCGCCTACGCCT
CGCGGTATCCGCAGCAGACCGATCGCCTGGTGGTGATGGACGCGCCCGTTCCGGGGATCCCGCCCTGGAACGAAATCGTC
CGCTCGCCGATGCTCTGGCATTTCGATTTCGGCGGGCCGGATGCAGAACGGCTGGTGGCCGGGCGTGAGCGCATCTACCT
CGACCGCTTCTGGAACGAGTTCGCCGGCGACCCGAAAAAAGTCGACGAGGCCACCCGCCAGCACTACGCCAAACTCTACG
CCCGCCCCGGCGCCATGCACGCGGCGTTCGCCCAGTTCCGTGCGATTCGCCAAGACGCGGTGGACAACGAAACGTCGATG
AAAACCAAACTGAAAATGCCGGTACTGGCGGTGGGCGGCGAGAAGTCCTTCGGCAACAACGAAGCCATGGTGATGCGCAA
TGCCGCCGACCACGTCACCGAATTGGTGATCGCCGACGCGGGGCATTGGCTGATGGAGGAAGCGCCCGTCGAAACCACCC
AAGCGATTCGCAGGTTTATTGCGCAGTGA

Upstream 100 bases:

>100_bases
TTGCCAAATTCCAATCTACAAACCGACCTGTGCTCATTTTTCACACTTCAGCTACAACAAATCCGTCTACGCTCCAGCCA
CCCCCTCTGGAGTGACCGCC

Downstream 100 bases:

>100_bases
ATGGCAAGCGAGTTGTTGTCATCGGTGCCGTTCAGCGCATCTCAGGCCACCGAGCTCAATCATTGACCGCATCAATAACA
ACCATTAATTCAATGAAGTT

Product: alpha/beta hydrolase fold protein

Products: NA

Alternate protein names: SEH; Cytosolic epoxide hydrolase; cEH; Epoxide hydratase [H]

Number of amino acids: Translated: 302; Mature: 302

Protein sequence:

>302_residues
MIRALLTASLLLAMSVPAHADVTPFPASFRTQDIAVDGAKIHTRIGGKGPAVVLLHGFGDTGDMWAPLAADLAKDHTVVV
PDLRGMGLSSIPADGYDKKTQAGDIRAVLASLGIEHSVVVGHDIGTMVAFAYASRYPQQTDRLVVMDAPVPGIPPWNEIV
RSPMLWHFDFGGPDAERLVAGRERIYLDRFWNEFAGDPKKVDEATRQHYAKLYARPGAMHAAFAQFRAIRQDAVDNETSM
KTKLKMPVLAVGGEKSFGNNEAMVMRNAADHVTELVIADAGHWLMEEAPVETTQAIRRFIAQ

Sequences:

>Translated_302_residues
MIRALLTASLLLAMSVPAHADVTPFPASFRTQDIAVDGAKIHTRIGGKGPAVVLLHGFGDTGDMWAPLAADLAKDHTVVV
PDLRGMGLSSIPADGYDKKTQAGDIRAVLASLGIEHSVVVGHDIGTMVAFAYASRYPQQTDRLVVMDAPVPGIPPWNEIV
RSPMLWHFDFGGPDAERLVAGRERIYLDRFWNEFAGDPKKVDEATRQHYAKLYARPGAMHAAFAQFRAIRQDAVDNETSM
KTKLKMPVLAVGGEKSFGNNEAMVMRNAADHVTELVIADAGHWLMEEAPVETTQAIRRFIAQ
>Mature_302_residues
MIRALLTASLLLAMSVPAHADVTPFPASFRTQDIAVDGAKIHTRIGGKGPAVVLLHGFGDTGDMWAPLAADLAKDHTVVV
PDLRGMGLSSIPADGYDKKTQAGDIRAVLASLGIEHSVVVGHDIGTMVAFAYASRYPQQTDRLVVMDAPVPGIPPWNEIV
RSPMLWHFDFGGPDAERLVAGRERIYLDRFWNEFAGDPKKVDEATRQHYAKLYARPGAMHAAFAQFRAIRQDAVDNETSM
KTKLKMPVLAVGGEKSFGNNEAMVMRNAADHVTELVIADAGHWLMEEAPVETTQAIRRFIAQ

Specific function: Involved in catabolic degradation of epoxides. Shows highest activity towards C6 and C7 carbocyclic epoxides. Also active towards linear 1,2-epoxyalkanes [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm. Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Epoxide hydrolase family [H]

Homologues:

Organism=Homo sapiens, GI218777837, Length=286, Percent_Identity=25.8741258741259, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI218777840, Length=282, Percent_Identity=23.4042553191489, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI13376164, Length=282, Percent_Identity=23.4042553191489, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI1787244, Length=100, Percent_Identity=35, Blast_Score=63, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17554012, Length=293, Percent_Identity=22.8668941979522, Blast_Score=79, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR000639
- InterPro:   IPR010497 [H]

Pfam domain/function: PF00561 Abhydrolase_1; PF06441 EHN [H]

EC number: =3.3.2.10 [H]

Molecular weight: Translated: 32919; Mature: 32919

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRALLTASLLLAMSVPAHADVTPFPASFRTQDIAVDGAKIHTRIGGKGPAVVLLHGFGD
CHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEECCCEEEEEECCCCCEEEEEECCCC
TGDMWAPLAADLAKDHTVVVPDLRGMGLSSIPADGYDKKTQAGDIRAVLASLGIEHSVVV
CCCCHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEE
GHDIGTMVAFAYASRYPQQTDRLVVMDAPVPGIPPWNEIVRSPMLWHFDFGGPDAERLVA
ECCHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCHHHHHCCCCEEEEECCCCCHHHHHH
GRERIYLDRFWNEFAGDPKKVDEATRQHYAKLYARPGAMHAAFAQFRAIRQDAVDNETSM
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHH
KTKLKMPVLAVGGEKSFGNNEAMVMRNAADHVTELVIADAGHWLMEEAPVETTQAIRRFI
HHEECCCEEEECCCCCCCCCCEEEEECHHHHHHHHHHHCCCCHHHHCCCCHHHHHHHHHH
AQ
CC
>Mature Secondary Structure
MIRALLTASLLLAMSVPAHADVTPFPASFRTQDIAVDGAKIHTRIGGKGPAVVLLHGFGD
CHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEECCCEEEEEECCCCCEEEEEECCCC
TGDMWAPLAADLAKDHTVVVPDLRGMGLSSIPADGYDKKTQAGDIRAVLASLGIEHSVVV
CCCCHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEE
GHDIGTMVAFAYASRYPQQTDRLVVMDAPVPGIPPWNEIVRSPMLWHFDFGGPDAERLVA
ECCHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCHHHHHCCCCEEEEECCCCCHHHHHH
GRERIYLDRFWNEFAGDPKKVDEATRQHYAKLYARPGAMHAAFAQFRAIRQDAVDNETSM
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHH
KTKLKMPVLAVGGEKSFGNNEAMVMRNAADHVTELVIADAGHWLMEEAPVETTQAIRRFI
HHEECCCEEEECCCCCCCCCCEEEEECHHHHHHHHHHHCCCCHHHHCCCCHHHHHHHHHH
AQ
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9578475 [H]