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The map label for this gene is arsB [H]

Identifier: 77458626

GI number: 77458626

Start: 2760659

End: 2761942

Strand: Reverse

Name: arsB [H]

Synonym: Pfl01_2399

Alternate gene names: 77458626

Gene position: 2761942-2760659 (Counterclockwise)

Preceding gene: 77458627

Following gene: 77458625

Centisome position: 42.9

GC content: 61.45

Gene sequence:

>1284_bases
ATGTTTTTTGCTTTTGCCATTTTTCTATTCACGATTGTGCTGGTCATCTGGCAGCCGAAGGGGCTGGGTGTCGGCTGGAG
TGCGACGCTCGGCGCGGTGCTGGCGCTGCTCAGCGGGGTTGTGACCCTGGGCGATATTCCCACGGTGTGGGGCATTGTCT
GGAACGCGACCGCGACGTTCATTGCCGTCATTATCATTAGCCTGTTGCTCGACGAAGCCGGTTTTTTCCAGTGGGCCGCG
TTGCACGTCGCCCGTTGGGCAAAGGGCGATGGCCGGCGTCTGTTCGTCTTTACGGTGTTGTTGGGCGCCGCCGTTTCTGC
GCTGTTTGCCAACGATGGCGCGGCGCTGATCCTGACGCCGATTGTGATTTCGATACTGCTCGCCCTGCGCTTCTCGGCGG
CTTCCACTTTGGCGTTCGTCATGGCGGCCGGTTTCATCGCCGATACCGCGAGCCTGCCACTGGTGGTGTCCAACCTGGTC
AACATCGTTTCCGCCGATTATTTCGGCCTTGGCTTCAGCGAATATGCCTCGGTGATGGTGCCGGTCAACGCGGTTGCCGT
GGCCTCCACTCTGGGGGTGCTGTACTGGTTCTTCCGGCGTGACATACCCACACATTACTCACTGGATGACTTGCAGGATC
CGCGACTGGCCATCCGCGACGGGCTGACTTTCCGGGTCGGCTGGATCGTGTTGGGGCTGCTCCTGATCGGTCTGTTCGCC
CTGGAACCGCTGGGCATTCCCATCAGTGCGATTGCCGCAGTCTGCGCGGCGCTGCTGTTCGCGGTGGCGGCGCGGGGTCA
TGTGATTTCGACCCGGCGCGTGCTGCGCGAAGCCCCTTGGCAGATCGTGATTTTCTCGCTCGGCATGTACCTGGTGGTGT
ACGGGCTGAAGAACGCCGGGCTGACGGATTATCTGGCGACGGCACTGAATTATCTGGCCGGTACCGGGCTCTGGGCCGCG
GCGCTGGGTACGGGATTTCTGACCGCCCTGCTGTCGTCCGCGATGAACAACATGCCGACAGTGTTGGTGGGCGCGTTGTC
GATCCAGGCCAGTGATGCCGTCGGCGTGATACGCGAGGCGATGATCTACGCCAATGTCATCGGCTCGGACCTGGGCCCGA
AAATCACCCCCATCGGCAGTCTGGCAACGCTGCTCTGGCTGCACGTGCTGGAGCGCAAAGGGATCCGCATCACCTGGGGT
TATTACTTCATGACCGGCGTGGTGCTGACCCTGCCGATTCTGTTCATCACCTTGTCTGCCCTGGCCCTGCGCCTGAGCGT
TTAA

Upstream 100 bases:

>100_bases
ACCGTCTGGCTGGAAGAGAACAGCGCGCGGCTGGCGGCCATGGGCGATCGCCCGCTCAACACCTCTGCTTGCTGCTAAGC
CTCAGGCTTTCGGGATTCCC

Downstream 100 bases:

>100_bases
CGCCTGTCTGGAAAGAGGAATCATCATGCGCGTTCTATTCATGTGCACCGCCAACAGCTGCCGCAGCATTCTGTCGGAGG
CGATGTTCAATCACTTGGCG

Product: arsenical pump membrane protein

Products: ADP; phosphate; arsenite [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 427; Mature: 427

Protein sequence:

>427_residues
MFFAFAIFLFTIVLVIWQPKGLGVGWSATLGAVLALLSGVVTLGDIPTVWGIVWNATATFIAVIIISLLLDEAGFFQWAA
LHVARWAKGDGRRLFVFTVLLGAAVSALFANDGAALILTPIVISILLALRFSAASTLAFVMAAGFIADTASLPLVVSNLV
NIVSADYFGLGFSEYASVMVPVNAVAVASTLGVLYWFFRRDIPTHYSLDDLQDPRLAIRDGLTFRVGWIVLGLLLIGLFA
LEPLGIPISAIAAVCAALLFAVAARGHVISTRRVLREAPWQIVIFSLGMYLVVYGLKNAGLTDYLATALNYLAGTGLWAA
ALGTGFLTALLSSAMNNMPTVLVGALSIQASDAVGVIREAMIYANVIGSDLGPKITPIGSLATLLWLHVLERKGIRITWG
YYFMTGVVLTLPILFITLSALALRLSV

Sequences:

>Translated_427_residues
MFFAFAIFLFTIVLVIWQPKGLGVGWSATLGAVLALLSGVVTLGDIPTVWGIVWNATATFIAVIIISLLLDEAGFFQWAA
LHVARWAKGDGRRLFVFTVLLGAAVSALFANDGAALILTPIVISILLALRFSAASTLAFVMAAGFIADTASLPLVVSNLV
NIVSADYFGLGFSEYASVMVPVNAVAVASTLGVLYWFFRRDIPTHYSLDDLQDPRLAIRDGLTFRVGWIVLGLLLIGLFA
LEPLGIPISAIAAVCAALLFAVAARGHVISTRRVLREAPWQIVIFSLGMYLVVYGLKNAGLTDYLATALNYLAGTGLWAA
ALGTGFLTALLSSAMNNMPTVLVGALSIQASDAVGVIREAMIYANVIGSDLGPKITPIGSLATLLWLHVLERKGIRITWG
YYFMTGVVLTLPILFITLSALALRLSV
>Mature_427_residues
MFFAFAIFLFTIVLVIWQPKGLGVGWSATLGAVLALLSGVVTLGDIPTVWGIVWNATATFIAVIIISLLLDEAGFFQWAA
LHVARWAKGDGRRLFVFTVLLGAAVSALFANDGAALILTPIVISILLALRFSAASTLAFVMAAGFIADTASLPLVVSNLV
NIVSADYFGLGFSEYASVMVPVNAVAVASTLGVLYWFFRRDIPTHYSLDDLQDPRLAIRDGLTFRVGWIVLGLLLIGLFA
LEPLGIPISAIAAVCAALLFAVAARGHVISTRRVLREAPWQIVIFSLGMYLVVYGLKNAGLTDYLATALNYLAGTGLWAA
ALGTGFLTALLSSAMNNMPTVLVGALSIQASDAVGVIREAMIYANVIGSDLGPKITPIGSLATLLWLHVLERKGIRITWG
YYFMTGVVLTLPILFITLSALALRLSV

Specific function: Involved in arsenical resistance. Thought to form the channel of an arsenite pump [H]

COG id: COG1055

COG function: function code P; Na+/H+ antiporter NhaD and related arsenite permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87082277, Length=426, Percent_Identity=74.8826291079812, Blast_Score=605, Evalue=1e-174,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000802 [H]

Pfam domain/function: PF02040 ArsB [H]

EC number: NA

Molecular weight: Translated: 45465; Mature: 45465

Theoretical pI: Translated: 8.87; Mature: 8.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHCC
>Mature Secondary Structure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HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; arsenite [Cytoplasm]; H2O [C]

Specific reaction: ATP + arsenite [Cytoplasm] + H2O = ADP + phosphate + arsenite [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3021763; 2523997; 1688427; 1704144 [H]