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The map label for this gene is nodW [H]

Identifier: 77458506

GI number: 77458506

Start: 2629350

End: 2629988

Strand: Reverse

Name: nodW [H]

Synonym: Pfl01_2279

Alternate gene names: 77458506

Gene position: 2629988-2629350 (Counterclockwise)

Preceding gene: 77458507

Following gene: 77458505

Centisome position: 40.85

GC content: 59.78

Gene sequence:

>639_bases
ATGGACGTAGAACCTGCACCCGAGCGAAACCCCGAATCCATCGTTTTCATCATTGATGATGACGCTCCTCTGCGTGAGGC
GCTTGGCAGCCTGTTACGCTCCATCGGCCTGCGGGTCGAGCTGTTCGGATCGGTCGCCGACTTCATGCAATACCAGCGAC
CTGACCTGCCCAGTTGCCTGGTGCTGGATGTGCGATTGCAAGGCACCAGCGGGCTGGTCTTTCAGCAGGCGCTCGCCGAC
GCCAACATCCATCTGCCGATCATTTTCATGACCGGGTACGGCGACATCGCCATGACCGTTCGCGCCATGAAAGCCGGTGC
CGTCGATTTCCTGGAAAAACCGTTTCGCGAGCAGGCCATGATCGACGCCGTGGTCGCCGCGCATACGCGGGACAGGTCGC
GCCGCGAGTACGAACTCCGCCATCATGAATTGCGCACACGCCATGGGACACTCACGGTCAGAGAGCAAGCGGTGATGGCG
CTGGCCGTCTCGGGTCTGATAAACAAGCAGATCGCCAGTGCCATCGGCCTGAGCGAGATCACCGTGAAAATTCACCGCAG
CCAGGCCATGCGCAAGATGCGCGCGCAGTCGTTTGCCGATCTGGTTCGAATGGCAGAAAGTCTGGGTATCAACCATTAG

Upstream 100 bases:

>100_bases
ACCCAAGAAAAATCCAATGAAAACCGGAGGCTCGGGCAAAGTTTTCCATTGGGTTTTTCCGCATCCTGCGCAACCTGATC
GCCGCGAAGCCATCCAGCGT

Downstream 100 bases:

>100_bases
ATTTAACGCCCAAATGCGGCGCACATTACTAGTCGTTTATATACGTTGGTATGACTTCTCCATACGCTGGCAGGATAAAA
CCAAACTTATGCAACATGGT

Product: two component LuxR family transcriptional regulator

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 212; Mature: 212

Protein sequence:

>212_residues
MDVEPAPERNPESIVFIIDDDAPLREALGSLLRSIGLRVELFGSVADFMQYQRPDLPSCLVLDVRLQGTSGLVFQQALAD
ANIHLPIIFMTGYGDIAMTVRAMKAGAVDFLEKPFREQAMIDAVVAAHTRDRSRREYELRHHELRTRHGTLTVREQAVMA
LAVSGLINKQIASAIGLSEITVKIHRSQAMRKMRAQSFADLVRMAESLGINH

Sequences:

>Translated_212_residues
MDVEPAPERNPESIVFIIDDDAPLREALGSLLRSIGLRVELFGSVADFMQYQRPDLPSCLVLDVRLQGTSGLVFQQALAD
ANIHLPIIFMTGYGDIAMTVRAMKAGAVDFLEKPFREQAMIDAVVAAHTRDRSRREYELRHHELRTRHGTLTVREQAVMA
LAVSGLINKQIASAIGLSEITVKIHRSQAMRKMRAQSFADLVRMAESLGINH
>Mature_212_residues
MDVEPAPERNPESIVFIIDDDAPLREALGSLLRSIGLRVELFGSVADFMQYQRPDLPSCLVLDVRLQGTSGLVFQQALAD
ANIHLPIIFMTGYGDIAMTVRAMKAGAVDFLEKPFREQAMIDAVVAAHTRDRSRREYELRHHELRTRHGTLTVREQAVMA
LAVSGLINKQIASAIGLSEITVKIHRSQAMRKMRAQSFADLVRMAESLGINH

Specific function: Member of the two-component regulatory system nodV/nodW probably involved in the regulation of the transcription of genes involved in the nodulation process [H]

COG id: COG4566

COG function: function code T; Response regulator

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=114, Percent_Identity=33.3333333333333, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1786747, Length=207, Percent_Identity=26.0869565217391, Blast_Score=67, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789
- InterPro:   IPR011990
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 23619; Mature: 23619

Theoretical pI: Translated: 7.24; Mature: 7.24

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDVEPAPERNPESIVFIIDDDAPLREALGSLLRSIGLRVELFGSVADFMQYQRPDLPSCL
CCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCEE
VLDVRLQGTSGLVFQQALADANIHLPIIFMTGYGDIAMTVRAMKAGAVDFLEKPFREQAM
EEEEEECCCCCHHHHHHHHCCCCEEEEEEEECCCHHHHHHHHHHHCHHHHHHHHHHHHHH
IDAVVAAHTRDRSRREYELRHHELRTRHGTLTVREQAVMALAVSGLINKQIASAIGLSEI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHCHHHH
TVKIHRSQAMRKMRAQSFADLVRMAESLGINH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MDVEPAPERNPESIVFIIDDDAPLREALGSLLRSIGLRVELFGSVADFMQYQRPDLPSCL
CCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCEE
VLDVRLQGTSGLVFQQALADANIHLPIIFMTGYGDIAMTVRAMKAGAVDFLEKPFREQAM
EEEEEECCCCCHHHHHHHHCCCCEEEEEEEECCCHHHHHHHHHHHCHHHHHHHHHHHHHH
IDAVVAAHTRDRSRREYELRHHELRTRHGTLTVREQAVMALAVSGLINKQIASAIGLSEI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHCHHHH
TVKIHRSQAMRKMRAQSFADLVRMAESLGINH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2320582; 11157954; 12597275 [H]