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The map label for this gene is nodW [H]

Identifier: 77458476

GI number: 77458476

Start: 2581404

End: 2582018

Strand: Reverse

Name: nodW [H]

Synonym: Pfl01_2249

Alternate gene names: 77458476

Gene position: 2582018-2581404 (Counterclockwise)

Preceding gene: 77458477

Following gene: 77458472

Centisome position: 40.1

GC content: 60.16

Gene sequence:

>615_bases
ATGAGTGAGGTCACGCCCGTCGTCTTCGTTGTCGATGACGATATTTCCGTGCGCGAGTCGCTGGAACTGATGATCCGCTT
CGCCGGATGGCAGCCACGACTGTTCGAGTCCGCGCAGGATTTTCTCGACGCGCCCAGAGCGCTGGTGCCCAGTTGCCTGG
TACTGGACATCAACCTGCCCGACCTGAGCGGACTGGATCTGCAGACCGCCCTGGCCGACGAACGCTACAACATGCCAATC
ATCTTCATCACCGGCTACGGCGATATTCCGCGCACGGTGCGGGCCCTGAAGGCCGGTGCGGTGGAGTTTCTGACCAAGCC
GTTCAACGACGACGTGATTCTCAGCGCCATGGCCGATGCGCTACAAAGCAGTCGTGTGGCGCTGGAAGGAGAAAAGAATC
TGCGTGCACTGCTTGAGGCTTACAAGACCCTGACGCCGCGCGAGCAGGAAATCATGGCTTCGGTGGTCAGCGGGCGACTC
AACAAACTGATTGCCGCCGACCTCAACATCAGCGAAATCACCGTCAAGGCCCATCGCGGCAAGGTCATGCGCAAGATGAA
GGCCCGTTCGCTGGCGGACCTGGTGAAAATGGCCGCCCTGATTACGCCGGAGTAG

Upstream 100 bases:

>100_bases
CGCCGCCCTGCCCCTCGATTGAGCGTGAAGCCGAACAGAACAAGGAATCGACATGTTGAACGCGAACAACGCAATCGCCC
CTTCCCGTGGAGCATTACCG

Downstream 100 bases:

>100_bases
CGTGCAGGCTCTGCCAGAAACTTTCCAGCCGACGCAGATCAAGGCTGTCGAAGCCTTCCGAAGCCCGCTCGCGACAGGCG
CCGAGCAACGTCCGGGCATG

Product: two component LuxR family transcriptional regulator

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 204; Mature: 203

Protein sequence:

>204_residues
MSEVTPVVFVVDDDISVRESLELMIRFAGWQPRLFESAQDFLDAPRALVPSCLVLDINLPDLSGLDLQTALADERYNMPI
IFITGYGDIPRTVRALKAGAVEFLTKPFNDDVILSAMADALQSSRVALEGEKNLRALLEAYKTLTPREQEIMASVVSGRL
NKLIAADLNISEITVKAHRGKVMRKMKARSLADLVKMAALITPE

Sequences:

>Translated_204_residues
MSEVTPVVFVVDDDISVRESLELMIRFAGWQPRLFESAQDFLDAPRALVPSCLVLDINLPDLSGLDLQTALADERYNMPI
IFITGYGDIPRTVRALKAGAVEFLTKPFNDDVILSAMADALQSSRVALEGEKNLRALLEAYKTLTPREQEIMASVVSGRL
NKLIAADLNISEITVKAHRGKVMRKMKARSLADLVKMAALITPE
>Mature_203_residues
SEVTPVVFVVDDDISVRESLELMIRFAGWQPRLFESAQDFLDAPRALVPSCLVLDINLPDLSGLDLQTALADERYNMPII
FITGYGDIPRTVRALKAGAVEFLTKPFNDDVILSAMADALQSSRVALEGEKNLRALLEAYKTLTPREQEIMASVVSGRLN
KLIAADLNISEITVKAHRGKVMRKMKARSLADLVKMAALITPE

Specific function: Member of the two-component regulatory system nodV/nodW probably involved in the regulation of the transcription of genes involved in the nodulation process [H]

COG id: COG4566

COG function: function code T; Response regulator

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=105, Percent_Identity=35.2380952380952, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1788521, Length=201, Percent_Identity=31.8407960199005, Blast_Score=65, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789
- InterPro:   IPR011990
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 22529; Mature: 22397

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEVTPVVFVVDDDISVRESLELMIRFAGWQPRLFESAQDFLDAPRALVPSCLVLDINLP
CCCCCEEEEEEECCCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHCCCEEEEEECCC
DLSGLDLQTALADERYNMPIIFITGYGDIPRTVRALKAGAVEFLTKPFNDDVILSAMADA
CCCCCCHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
LQSSRVALEGEKNLRALLEAYKTLTPREQEIMASVVSGRLNKLIAADLNISEITVKAHRG
HHHCCEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHEEEEHHHH
KVMRKMKARSLADLVKMAALITPE
HHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SEVTPVVFVVDDDISVRESLELMIRFAGWQPRLFESAQDFLDAPRALVPSCLVLDINLP
CCCCEEEEEEECCCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHCCCEEEEEECCC
DLSGLDLQTALADERYNMPIIFITGYGDIPRTVRALKAGAVEFLTKPFNDDVILSAMADA
CCCCCCHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
LQSSRVALEGEKNLRALLEAYKTLTPREQEIMASVVSGRLNKLIAADLNISEITVKAHRG
HHHCCEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHEEEEHHHH
KVMRKMKARSLADLVKMAALITPE
HHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2320582; 11157954; 12597275 [H]