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The map label for this gene is gyaR [H]

Identifier: 77458460

GI number: 77458460

Start: 2562994

End: 2563950

Strand: Reverse

Name: gyaR [H]

Synonym: Pfl01_2233

Alternate gene names: 77458460

Gene position: 2563950-2562994 (Counterclockwise)

Preceding gene: 77458461

Following gene: 77458457

Centisome position: 39.82

GC content: 62.9

Gene sequence:

>957_bases
ATGAAGCCAGAAGTCTTGCAGCTCAGCCCGATCCTGATTCCGCAAATCAATGCCCGTCTCGGCGAACTGTTCACGATCAG
GCGTTATTTCGAGCAGGCCGACAAAGCGGCGTATTTGCAGGAACACGGCGCCAACATTCGCGGCGTGATCACCGGTGGTC
ACACGGGCATCAGTCAGGCGTTGATGGCGCAACTGCCCAAACTGGAAGTGGTGGCGGTCAACGGTGTCGGCACCGATGCA
GTAGACCTGGCCTACGCCAGGGATCGCGGAATCCGCGTGACCGCGACCATCGGCGCACTGACCGAAGACGTGGCCGACCT
GGCCATCGGTTTGCTGATCGCCGTGTGTCGTGGGCTGTGTACCAGTGACCGTTACGTGCGCTCGGGCCAGTGGCCGCAAA
GCCCGACCCCATTGGCACCGCTGCCTTTGGCGCGGCAAGTGTCCGGCATGCGTATCGGCATCGTCGGCATGGGCCGGGTT
GGCCGAGCGGTCGCCACTCGCGCTGCCGCGTTTGGCTGTCCGATCAGTTACACCGACCTGCAACCAATGAGCGATGTCAG
CCACACCTTCATCGCCGACCTCAAGCAATTGGCCCGCGACAGTGATGCCCTGATTCTCGCGGCAGCCGCCGACAAGGCCG
AGGCCATCATCGATGCCTCCGTGCTGCAGGCTCTGGGCAAGGGCGGCTACCTGATCAACGTCGCCCGAGGAAAACTGGTC
AACGAAACCGATCTGGTGGCGGCCCTGACGGCTGGCGAGATCGCGGGTGCGGCGCTGGATGTTTTCGTCGACGAGCCTAA
CGTGCCTGAAGCCTTGTTTGCCCAGGAACAAGTGGTGCTGCAACCACATCGCGCCAGCGCGACCCTGCAGACCCGCACCC
GCATGGGCGAGATGGTTGTGGCGAGCCTGGTGGACAGTTTTGCCGGAAAGACTCCGCAAGGATGTGTGACCGGCTGA

Upstream 100 bases:

>100_bases
CCCTGTCGGCCAACGTCCTGAACATGACCGTCATGGCCACTGCCATGCCGTTTGCCGGCCGTGGTTGAGCCGGCTTTTTT
ATTGCGTAAGTGAGGTTTAC

Downstream 100 bases:

>100_bases
GCCTCACAGTCATCGGGGGCCGCTGCCCCCGATGATGGATTTCAGACCCGATCGGACTACAACGTGTAGGAAATGCCGAC
CTGCACCGTTCTCGGTGCCC

Product: D-isomer specific 2-hydroxyacid dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 318; Mature: 318

Protein sequence:

>318_residues
MKPEVLQLSPILIPQINARLGELFTIRRYFEQADKAAYLQEHGANIRGVITGGHTGISQALMAQLPKLEVVAVNGVGTDA
VDLAYARDRGIRVTATIGALTEDVADLAIGLLIAVCRGLCTSDRYVRSGQWPQSPTPLAPLPLARQVSGMRIGIVGMGRV
GRAVATRAAAFGCPISYTDLQPMSDVSHTFIADLKQLARDSDALILAAAADKAEAIIDASVLQALGKGGYLINVARGKLV
NETDLVAALTAGEIAGAALDVFVDEPNVPEALFAQEQVVLQPHRASATLQTRTRMGEMVVASLVDSFAGKTPQGCVTG

Sequences:

>Translated_318_residues
MKPEVLQLSPILIPQINARLGELFTIRRYFEQADKAAYLQEHGANIRGVITGGHTGISQALMAQLPKLEVVAVNGVGTDA
VDLAYARDRGIRVTATIGALTEDVADLAIGLLIAVCRGLCTSDRYVRSGQWPQSPTPLAPLPLARQVSGMRIGIVGMGRV
GRAVATRAAAFGCPISYTDLQPMSDVSHTFIADLKQLARDSDALILAAAADKAEAIIDASVLQALGKGGYLINVARGKLV
NETDLVAALTAGEIAGAALDVFVDEPNVPEALFAQEQVVLQPHRASATLQTRTRMGEMVVASLVDSFAGKTPQGCVTG
>Mature_318_residues
MKPEVLQLSPILIPQINARLGELFTIRRYFEQADKAAYLQEHGANIRGVITGGHTGISQALMAQLPKLEVVAVNGVGTDA
VDLAYARDRGIRVTATIGALTEDVADLAIGLLIAVCRGLCTSDRYVRSGQWPQSPTPLAPLPLARQVSGMRIGIVGMGRV
GRAVATRAAAFGCPISYTDLQPMSDVSHTFIADLKQLARDSDALILAAAADKAEAIIDASVLQALGKGGYLINVARGKLV
NETDLVAALTAGEIAGAALDVFVDEPNVPEALFAQEQVVLQPHRASATLQTRTRMGEMVVASLVDSFAGKTPQGCVTG

Specific function: Unknown

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. GyaR subfamily [H]

Homologues:

Organism=Homo sapiens, GI6912396, Length=253, Percent_Identity=34.3873517786561, Blast_Score=125, Evalue=7e-29,
Organism=Homo sapiens, GI23308577, Length=277, Percent_Identity=29.6028880866426, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI4557497, Length=271, Percent_Identity=28.7822878228782, Blast_Score=95, Evalue=8e-20,
Organism=Homo sapiens, GI61743967, Length=271, Percent_Identity=28.7822878228782, Blast_Score=95, Evalue=8e-20,
Organism=Homo sapiens, GI145580578, Length=271, Percent_Identity=28.7822878228782, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI4557499, Length=271, Percent_Identity=28.7822878228782, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI145580575, Length=265, Percent_Identity=29.4339622641509, Blast_Score=89, Evalue=6e-18,
Organism=Escherichia coli, GI87082289, Length=327, Percent_Identity=33.0275229357798, Blast_Score=138, Evalue=5e-34,
Organism=Escherichia coli, GI1789279, Length=259, Percent_Identity=26.6409266409266, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI87081824, Length=161, Percent_Identity=32.9192546583851, Blast_Score=69, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17532191, Length=278, Percent_Identity=29.4964028776978, Blast_Score=103, Evalue=9e-23,
Organism=Caenorhabditis elegans, GI25147481, Length=260, Percent_Identity=29.2307692307692, Blast_Score=93, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6324055, Length=298, Percent_Identity=28.1879194630872, Blast_Score=112, Evalue=6e-26,
Organism=Saccharomyces cerevisiae, GI6322116, Length=315, Percent_Identity=26.031746031746, Blast_Score=99, Evalue=7e-22,
Organism=Saccharomyces cerevisiae, GI6320925, Length=264, Percent_Identity=28.030303030303, Blast_Score=94, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6324964, Length=229, Percent_Identity=25.3275109170306, Blast_Score=76, Evalue=6e-15,
Organism=Drosophila melanogaster, GI28574284, Length=282, Percent_Identity=30.8510638297872, Blast_Score=118, Evalue=5e-27,
Organism=Drosophila melanogaster, GI45551003, Length=282, Percent_Identity=30.8510638297872, Blast_Score=118, Evalue=6e-27,
Organism=Drosophila melanogaster, GI24585514, Length=282, Percent_Identity=30.8510638297872, Blast_Score=118, Evalue=6e-27,
Organism=Drosophila melanogaster, GI28574282, Length=282, Percent_Identity=30.8510638297872, Blast_Score=118, Evalue=6e-27,
Organism=Drosophila melanogaster, GI45552429, Length=282, Percent_Identity=30.8510638297872, Blast_Score=118, Evalue=6e-27,
Organism=Drosophila melanogaster, GI28574286, Length=277, Percent_Identity=32.8519855595668, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI28571528, Length=259, Percent_Identity=32.4324324324324, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24585516, Length=251, Percent_Identity=29.4820717131474, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24646446, Length=277, Percent_Identity=29.9638989169675, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24646448, Length=277, Percent_Identity=29.9638989169675, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24646452, Length=277, Percent_Identity=29.9638989169675, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24646450, Length=277, Percent_Identity=29.9638989169675, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI62472511, Length=273, Percent_Identity=29.3040293040293, Blast_Score=94, Evalue=9e-20,
Organism=Drosophila melanogaster, GI19921140, Length=279, Percent_Identity=25.4480286738351, Blast_Score=82, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.26 [H]

Molecular weight: Translated: 33433; Mature: 33433

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: PS00065 D_2_HYDROXYACID_DH_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPEVLQLSPILIPQINARLGELFTIRRYFEQADKAAYLQEHGANIRGVITGGHTGISQA
CCCCCEEECCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHH
LMAQLPKLEVVAVNGVGTDAVDLAYARDRGIRVTATIGALTEDVADLAIGLLIAVCRGLC
HHHHCCCEEEEEECCCCCCHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHCC
TSDRYVRSGQWPQSPTPLAPLPLARQVSGMRIGIVGMGRVGRAVATRAAAFGCPISYTDL
CCCCHHCCCCCCCCCCCCCCCCHHHHCCCCEEEEEECCHHHHHHHHHHHHHCCCCCCCCC
QPMSDVSHTFIADLKQLARDSDALILAAAADKAEAIIDASVLQALGKGGYLINVARGKLV
CCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEECCCCCC
NETDLVAALTAGEIAGAALDVFVDEPNVPEALFAQEQVVLQPHRASATLQTRTRMGEMVV
CCHHHHEEHHHHHHCCCEEEEEECCCCCCHHHHHCCHHEECCCCCCHHHHHHHHHHHHHH
ASLVDSFAGKTPQGCVTG
HHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MKPEVLQLSPILIPQINARLGELFTIRRYFEQADKAAYLQEHGANIRGVITGGHTGISQA
CCCCCEEECCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHH
LMAQLPKLEVVAVNGVGTDAVDLAYARDRGIRVTATIGALTEDVADLAIGLLIAVCRGLC
HHHHCCCEEEEEECCCCCCHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHCC
TSDRYVRSGQWPQSPTPLAPLPLARQVSGMRIGIVGMGRVGRAVATRAAAFGCPISYTDL
CCCCHHCCCCCCCCCCCCCCCCHHHHCCCCEEEEEECCHHHHHHHHHHHHHCCCCCCCCC
QPMSDVSHTFIADLKQLARDSDALILAAAADKAEAIIDASVLQALGKGGYLINVARGKLV
CCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEECCCCCC
NETDLVAALTAGEIAGAALDVFVDEPNVPEALFAQEQVVLQPHRASATLQTRTRMGEMVV
CCHHHHEEHHHHHHCCCEEEEEECCCCCCHHHHHCCHHEECCCCCCHHHHHHHHHHHHHH
ASLVDSFAGKTPQGCVTG
HHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA