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The map label for this gene is fabI [H]

Identifier: 77458404

GI number: 77458404

Start: 2458461

End: 2459255

Strand: Reverse

Name: fabI [H]

Synonym: Pfl01_2177

Alternate gene names: 77458404

Gene position: 2459255-2458461 (Counterclockwise)

Preceding gene: 77458405

Following gene: 77458399

Centisome position: 38.2

GC content: 62.26

Gene sequence:

>795_bases
ATGGGTTTTCTCGCCGGTAAGCGCGTACTGATCGTCGGTGTCGCCAGCAAGCTGTCCATCGCATCCGGCATCGCTGCCGC
CATGCATCGCGAGGGCGCTGAGCTTGCCTTCACTTATCAGAACGACAAACTGAAAGGTCGCGTCGAAGAGTTCGCACAGG
GCTGGGGTTCGAGCCCGGAGCTGTGCTTCCCGTGCGACGTGGCCAGCGATGAAGAAATCGCCAAGGTCTTCGAAGAACTG
AGCAAGAAGTGGGACGGCCTGGACTGCATCGTGCACTCCGTCGGCTTCGCCCCGGGCGACCAACTGGACGGCGACTTCAC
CGAAGCCACCACCCGTGAAGGCTTCCGCATCGCTCACGACATCAGCGCCTACAGCTTCGTGGCTCTGGCCAAAGCCGGTC
GCGAAATGATGAAAGGCCGCAATGGTAGCCTGCTGACTCTGTCGTATCTGGGCGCCGAGCGCACCATGCCGAACTACAAC
GTGATGGGCATGGCCAAGGCTTCGCTGGAAGCCGGCGTACGTTACCTGGCCGGCTCCCTGGGCCCGGACGGTACTCGCGT
CAACTGCGTATCGGCCGGTCCGATCCGCACCCTGGCAGCTTCCGGCATCAAGAACTTCCGCAAGATGCTGGCCGCCAACG
AAGCGCAAACCCCGCTGCGCCGCAACGTCACCATCGAAGAAGTCGGCAACGCCGGCGCCTTCCTGTGCTCCGACCTGGCG
TCGGGCATCAGCGGTGAAATCATGTACGTGGACGGCGGCTTCAACACCACCGCCATGGGCAACATCGAAGAGTAA

Upstream 100 bases:

>100_bases
AGTATCTTTGCCGCCCCCCAACATTCGTATACACAGCAGTTGCTGGAGGCCGCTTTCCTGGCACCAGCCACTGCGCAATA
ACCTGAAAGAGGAGCAACAC

Downstream 100 bases:

>100_bases
TCTTCGCGCTGCCGATAAAAAGCCCGCCTATTCAGGCGGGCTTTTTCATGCCTGCGATTTCGTTAACATCGTCTTTTTCT
GTGGGAGCCAGCCTGCTGGC

Product: enoyl-[acyl-carrier-protein] reductase

Products: NA

Alternate protein names: NADH-dependent enoyl-ACP reductase [H]

Number of amino acids: Translated: 264; Mature: 263

Protein sequence:

>264_residues
MGFLAGKRVLIVGVASKLSIASGIAAAMHREGAELAFTYQNDKLKGRVEEFAQGWGSSPELCFPCDVASDEEIAKVFEEL
SKKWDGLDCIVHSVGFAPGDQLDGDFTEATTREGFRIAHDISAYSFVALAKAGREMMKGRNGSLLTLSYLGAERTMPNYN
VMGMAKASLEAGVRYLAGSLGPDGTRVNCVSAGPIRTLAASGIKNFRKMLAANEAQTPLRRNVTIEEVGNAGAFLCSDLA
SGISGEIMYVDGGFNTTAMGNIEE

Sequences:

>Translated_264_residues
MGFLAGKRVLIVGVASKLSIASGIAAAMHREGAELAFTYQNDKLKGRVEEFAQGWGSSPELCFPCDVASDEEIAKVFEEL
SKKWDGLDCIVHSVGFAPGDQLDGDFTEATTREGFRIAHDISAYSFVALAKAGREMMKGRNGSLLTLSYLGAERTMPNYN
VMGMAKASLEAGVRYLAGSLGPDGTRVNCVSAGPIRTLAASGIKNFRKMLAANEAQTPLRRNVTIEEVGNAGAFLCSDLA
SGISGEIMYVDGGFNTTAMGNIEE
>Mature_263_residues
GFLAGKRVLIVGVASKLSIASGIAAAMHREGAELAFTYQNDKLKGRVEEFAQGWGSSPELCFPCDVASDEEIAKVFEELS
KKWDGLDCIVHSVGFAPGDQLDGDFTEATTREGFRIAHDISAYSFVALAKAGREMMKGRNGSLLTLSYLGAERTMPNYNV
MGMAKASLEAGVRYLAGSLGPDGTRVNCVSAGPIRTLAASGIKNFRKMLAANEAQTPLRRNVTIEEVGNAGAFLCSDLAS
GISGEIMYVDGGFNTTAMGNIEE

Specific function: Fatty acid biosynthesis pathway; second reduction step. [C]

COG id: COG0623

COG function: function code I; Enoyl-[acyl-carrier-protein] reductase (NADH)

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family. FabI subfamily [H]

Homologues:

Organism=Homo sapiens, GI4503301, Length=222, Percent_Identity=28.3783783783784, Blast_Score=68, Evalue=7e-12,
Organism=Escherichia coli, GI1787545, Length=263, Percent_Identity=69.9619771863118, Blast_Score=377, Evalue=1e-106,
Organism=Escherichia coli, GI1787905, Length=254, Percent_Identity=29.1338582677165, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI2367365, Length=266, Percent_Identity=30.0751879699248, Blast_Score=62, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17531453, Length=267, Percent_Identity=27.3408239700375, Blast_Score=67, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI115534694, Length=257, Percent_Identity=28.0155642023346, Blast_Score=67, Evalue=8e-12,
Organism=Drosophila melanogaster, GI24644339, Length=258, Percent_Identity=27.906976744186, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24644337, Length=200, Percent_Identity=31, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI21357041, Length=251, Percent_Identity=27.4900398406374, Blast_Score=65, Evalue=4e-11,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR014358
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.3.1.9 [H]

Molecular weight: Translated: 28039; Mature: 27908

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGFLAGKRVLIVGVASKLSIASGIAAAMHREGAELAFTYQNDKLKGRVEEFAQGWGSSPE
CCCCCCCEEEEEEECHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHCCCCCC
LCFPCDVASDEEIAKVFEELSKKWDGLDCIVHSVGFAPGDQLDGDFTEATTREGFRIAHD
EEEECCCCCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCHHHHHHCCCEEEEHH
ISAYSFVALAKAGREMMKGRNGSLLTLSYLGAERTMPNYNVMGMAKASLEAGVRYLAGSL
HHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCEEEEHHHHHHHHHHHHHCCC
GPDGTRVNCVSAGPIRTLAASGIKNFRKMLAANEAQTPLRRNVTIEEVGNAGAFLCSDLA
CCCCCEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCHHHHCCCHHHHHHHHH
SGISGEIMYVDGGFNTTAMGNIEE
CCCCCEEEEEECCCCCCCCCCCCC
>Mature Secondary Structure 
GFLAGKRVLIVGVASKLSIASGIAAAMHREGAELAFTYQNDKLKGRVEEFAQGWGSSPE
CCCCCCEEEEEEECHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHCCCCCC
LCFPCDVASDEEIAKVFEELSKKWDGLDCIVHSVGFAPGDQLDGDFTEATTREGFRIAHD
EEEECCCCCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCHHHHHHCCCEEEEHH
ISAYSFVALAKAGREMMKGRNGSLLTLSYLGAERTMPNYNVMGMAKASLEAGVRYLAGSL
HHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCEEEEHHHHHHHHHHHHHCCC
GPDGTRVNCVSAGPIRTLAASGIKNFRKMLAANEAQTPLRRNVTIEEVGNAGAFLCSDLA
CCCCCEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCHHHHCCCHHHHHHHHH
SGISGEIMYVDGGFNTTAMGNIEE
CCCCCEEEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10464225; 10984043 [H]