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The map label for this gene is spoT [H]

Identifier: 77458360

GI number: 77458360

Start: 2417518

End: 2417940

Strand: Direct

Name: spoT [H]

Synonym: Pfl01_2133

Alternate gene names: 77458360

Gene position: 2417518-2417940 (Clockwise)

Preceding gene: 77458359

Following gene: 77458361

Centisome position: 37.55

GC content: 62.65

Gene sequence:

>423_bases
ATGACTCAGACTCTGGAACGCGCCATCGCGATTGCCGCCACGGCTCACGCGGGGCAGCTGGACAAGGGCGGTGCGCCGTA
TATTTTGCATCCGCTGAAGGTCATGTTGCGCATGAGCAGCCTGGAAGAGCGCATCGTCGCGGTGTTGCACGATGTGGTCG
AGGACTGCGGCATCAGCCTGGAGGATTTGCGCAAGGAAGGCTTCAGCGAAGCCGTTTTGGCCGCCATCGAAGCGGTGACC
AAAGTGCCCGGCGAGTCCTACGAGGACTTCGTCGAGCGCGCGGCGCAGAACCCGATCGGGCGGGTGGTGAAACTGGCGGA
TCTGGAAGAGAACAGCGACCTGTCGCGGATTGCCTCGCCGAGTTGGGAGGATCTGGAGCGGATCGAAAAATATCGGCGGG
CGATTGGACGGCTGCGCGCCTGA

Upstream 100 bases:

>100_bases
GCCGGCGCAGCCAGCCGGACGTTTGCAGGCGATTGGGCGCTGGTTCAAACGTCATTTCTATATTGGCGGCCGGTACGGCG
TCTGATCCGAGGAAACGCCC

Downstream 100 bases:

>100_bases
CTTAACTGAAAAGCACCTTGAATAGCCAGGAAGGCGACCATGAAATCCATTCTGTGTTTGTTGATTGCTGCCGGCTTCGG
CGCGCTGTTGCAGTTCGAAG

Product: metal dependent phosphohydrolase

Products: NA

Alternate protein names: Penta-phosphate guanosine-3'-pyrophosphohydrolase; (ppGpp)ase [H]

Number of amino acids: Translated: 140; Mature: 139

Protein sequence:

>140_residues
MTQTLERAIAIAATAHAGQLDKGGAPYILHPLKVMLRMSSLEERIVAVLHDVVEDCGISLEDLRKEGFSEAVLAAIEAVT
KVPGESYEDFVERAAQNPIGRVVKLADLEENSDLSRIASPSWEDLERIEKYRRAIGRLRA

Sequences:

>Translated_140_residues
MTQTLERAIAIAATAHAGQLDKGGAPYILHPLKVMLRMSSLEERIVAVLHDVVEDCGISLEDLRKEGFSEAVLAAIEAVT
KVPGESYEDFVERAAQNPIGRVVKLADLEENSDLSRIASPSWEDLERIEKYRRAIGRLRA
>Mature_139_residues
TQTLERAIAIAATAHAGQLDKGGAPYILHPLKVMLRMSSLEERIVAVLHDVVEDCGISLEDLRKEGFSEAVLAAIEAVTK
VPGESYEDFVERAAQNPIGRVVKLADLEENSDLSRIASPSWEDLERIEKYRRAIGRLRA

Specific function: In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the degradation of ppGpp into G

COG id: COG0317

COG function: function code TK; Guanosine polyphosphate pyrophosphohydrolases/synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HD domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR012675
- InterPro:   IPR003607
- InterPro:   IPR007685
- InterPro:   IPR004811
- InterPro:   IPR004095
- InterPro:   IPR012676 [H]

Pfam domain/function: PF01842 ACT; PF04607 RelA_SpoT; PF02824 TGS [H]

EC number: =3.1.7.2 [H]

Molecular weight: Translated: 15468; Mature: 15337

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQTLERAIAIAATAHAGQLDKGGAPYILHPLKVMLRMSSLEERIVAVLHDVVEDCGISL
CCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
EDLRKEGFSEAVLAAIEAVTKVPGESYEDFVERAAQNPIGRVVKLADLEENSDLSRIASP
HHHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHCCCCCCCHHHHCCC
SWEDLERIEKYRRAIGRLRA
CHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TQTLERAIAIAATAHAGQLDKGGAPYILHPLKVMLRMSSLEERIVAVLHDVVEDCGISL
CHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
EDLRKEGFSEAVLAAIEAVTKVPGESYEDFVERAAQNPIGRVVKLADLEENSDLSRIASP
HHHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHCCCCCCCHHHHCCC
SWEDLERIEKYRRAIGRLRA
CHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]