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The map label for this gene is prpB [C]

Identifier: 77458279

GI number: 77458279

Start: 2339236

End: 2340105

Strand: Reverse

Name: prpB [C]

Synonym: Pfl01_2052

Alternate gene names: 77458279

Gene position: 2340105-2339236 (Counterclockwise)

Preceding gene: 77458280

Following gene: 77458275

Centisome position: 36.35

GC content: 62.87

Gene sequence:

>870_bases
ATGACCAGGCTTTCTCATCAAGATTTGCGCCGTAACTTCCGTCAGCTGCTGGCTTCCGACACCTGCTATCACACCGCCTC
GGTGTTCGATCCGATGTCCGCCCGCATCGCCGCTGACCTGGGTTTTGAAGTAGGGATTCTCGGCGGCTCGGTCGCCTCAT
TGCAGGTGCTGGGCGCTCCGGACTTTGCCCTGATCACCCTCAGCGAATTCGCTGAACAGGCCACCCGCATCGGCCGCGTC
GCCCAATTGCCGGTGATCGCCGACGCCGACCACGGCTACGGCAACGCGCTCAACGTAATGCGCACCATCGTCGAGCTGGA
ACGCGCCGGCGTCGCCGCGCTGACCATCGAAGACACCCTGCTGCCCGCCCAGTTCGGCCGCAAATCCACCGACCTGATCA
CCGTCGCCGAAGGCGTGGGCAAGATCCGCGCGGCACTGGAAGCCCGAGTCGATTCGGAAATGGCGATCATCGCCCGGACC
AACGCCGGTATCCTGCCGAACCAGGAAATCATCAGCCGCACCAAGCAATACCAGGCCGCAGGCGCCGACGGTATCTGCAT
GGTCGGTATTCAGGACTTCGACCAGCTCGAACAGATCGCCGAGCACCTGACCGTCCCGCTGATGCTGGTGACCTACGGCA
ACCCGGCGCTGCGCGACGACAAACGCCTGGCCGAACTGGGTGTGCGCGTGACCATCGACGGCCACGGTGCTTACTTCGCG
GCGATCAAGGCGACTTACGACAGCCTGCGCGAACAGCGCCAGATCTTCACCCAGGCCTCCGACCTGAGCGCCACCGAACT
GACCCACACCTACACCCAGCCTGAGGAATACATCCTCTGGGCCAAGGAATACATGAGCGTCAAAGAGTAA

Upstream 100 bases:

>100_bases
TTCGTCATGATCCTTGACCCATTGGCGTGATAGCCTTTTCACCTTCGGCGTTGACACCTTTGCTCAAGCCTTTCCATTCC
TTTGTATTGGTGGAACTCGT

Downstream 100 bases:

>100_bases
CCTCGCACCACGCTACAAAGAGGCAGAGCGGCCCGACGCCATCACGAGTCCACTCGCATGGCGCCCGCCGCGAAACCGCT
AGAGCGCTGTCTTGAAGCCT

Product: 2,3-dimethylmalate lyase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 289; Mature: 288

Protein sequence:

>289_residues
MTRLSHQDLRRNFRQLLASDTCYHTASVFDPMSARIAADLGFEVGILGGSVASLQVLGAPDFALITLSEFAEQATRIGRV
AQLPVIADADHGYGNALNVMRTIVELERAGVAALTIEDTLLPAQFGRKSTDLITVAEGVGKIRAALEARVDSEMAIIART
NAGILPNQEIISRTKQYQAAGADGICMVGIQDFDQLEQIAEHLTVPLMLVTYGNPALRDDKRLAELGVRVTIDGHGAYFA
AIKATYDSLREQRQIFTQASDLSATELTHTYTQPEEYILWAKEYMSVKE

Sequences:

>Translated_289_residues
MTRLSHQDLRRNFRQLLASDTCYHTASVFDPMSARIAADLGFEVGILGGSVASLQVLGAPDFALITLSEFAEQATRIGRV
AQLPVIADADHGYGNALNVMRTIVELERAGVAALTIEDTLLPAQFGRKSTDLITVAEGVGKIRAALEARVDSEMAIIART
NAGILPNQEIISRTKQYQAAGADGICMVGIQDFDQLEQIAEHLTVPLMLVTYGNPALRDDKRLAELGVRVTIDGHGAYFA
AIKATYDSLREQRQIFTQASDLSATELTHTYTQPEEYILWAKEYMSVKE
>Mature_288_residues
TRLSHQDLRRNFRQLLASDTCYHTASVFDPMSARIAADLGFEVGILGGSVASLQVLGAPDFALITLSEFAEQATRIGRVA
QLPVIADADHGYGNALNVMRTIVELERAGVAALTIEDTLLPAQFGRKSTDLITVAEGVGKIRAALEARVDSEMAIIARTN
AGILPNQEIISRTKQYQAAGADGICMVGIQDFDQLEQIAEHLTVPLMLVTYGNPALRDDKRLAELGVRVTIDGHGAYFAA
IKATYDSLREQRQIFTQASDLSATELTHTYTQPEEYILWAKEYMSVKE

Specific function: Catalyzes the decarboxylation of oxaloacetate into pyruvate. Seems to play a role in maintaining cellular concentrations of bicarbonate and pyruvate

COG id: COG2513

COG function: function code G; PEP phosphonomutase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate lyase/PEP mutase superfamily. Oxaloacetate decarboxylase family

Homologues:

Organism=Escherichia coli, GI1786525, Length=201, Percent_Identity=30.8457711442786, Blast_Score=89, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): OADC1_PSEPF (Q3KEL1)

Other databases:

- EMBL:   CP000094
- RefSeq:   YP_347784.1
- ProteinModelPortal:   Q3KEL1
- SMR:   Q3KEL1
- STRING:   Q3KEL1
- GeneID:   3716062
- GenomeReviews:   CP000094_GR
- KEGG:   pfo:Pfl01_2052
- eggNOG:   COG2513
- HOGENOM:   HBG728656
- OMA:   VDADHGY
- ProtClustDB:   CLSK868955
- BioCyc:   PFLU205922:PFL_2052-MONOMER
- HAMAP:   MF_01299
- InterPro:   IPR000918
- InterPro:   IPR015813
- Gene3D:   G3DSA:3.20.20.60

Pfam domain/function: PF00463 ICL; SSF51621 Pyrv/PenolPyrv_Kinase_cat

EC number: =4.1.1.3

Molecular weight: Translated: 31586; Mature: 31455

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: NA

Important sites: BINDING 50-50 BINDING 159-159 BINDING 235-235

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRLSHQDLRRNFRQLLASDTCYHTASVFDPMSARIAADLGFEVGILGGSVASLQVLGAP
CCCCCHHHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHCCCEEEEECCCEEEEEEECCC
DFALITLSEFAEQATRIGRVAQLPVIADADHGYGNALNVMRTIVELERAGVAALTIEDTL
CEEEEEHHHHHHHHHHHCCHHCCCEEEECCCCCCHHHHHHHHHHHHHHCCEEEEEEECHH
LPAQFGRKSTDLITVAEGVGKIRAALEARVDSEMAIIARTNAGILPNQEIISRTKQYQAA
CHHHHCCCCCCEEEHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHC
GADGICMVGIQDFDQLEQIAEHLTVPLMLVTYGNPALRDDKRLAELGVRVTIDGHGAYFA
CCCCEEEECCCCHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHCCCEEEEECCCEEEH
AIKATYDSLREQRQIFTQASDLSATELTHTYTQPEEYILWAKEYMSVKE
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCHHHEEEHHHHHCCCC
>Mature Secondary Structure 
TRLSHQDLRRNFRQLLASDTCYHTASVFDPMSARIAADLGFEVGILGGSVASLQVLGAP
CCCCHHHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHCCCEEEEECCCEEEEEEECCC
DFALITLSEFAEQATRIGRVAQLPVIADADHGYGNALNVMRTIVELERAGVAALTIEDTL
CEEEEEHHHHHHHHHHHCCHHCCCEEEECCCCCCHHHHHHHHHHHHHHCCEEEEEEECHH
LPAQFGRKSTDLITVAEGVGKIRAALEARVDSEMAIIARTNAGILPNQEIISRTKQYQAA
CHHHHCCCCCCEEEHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHC
GADGICMVGIQDFDQLEQIAEHLTVPLMLVTYGNPALRDDKRLAELGVRVTIDGHGAYFA
CCCCEEEECCCCHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHCCCEEEEECCCEEEH
AIKATYDSLREQRQIFTQASDLSATELTHTYTQPEEYILWAKEYMSVKE
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCHHHEEEHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA