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The map label for this gene is cpg2 [H]

Identifier: 77458277

GI number: 77458277

Start: 2337072

End: 2338301

Strand: Direct

Name: cpg2 [H]

Synonym: Pfl01_2050

Alternate gene names: 77458277

Gene position: 2337072-2338301 (Clockwise)

Preceding gene: 77458276

Following gene: 77458278

Centisome position: 36.3

GC content: 62.11

Gene sequence:

>1230_bases
ATGACGTTCAAATTCCGTCGTTCTCTGCTGGCCGGCCTGTCCCTCACTTTTGCAGCCTCCGGTGTCTTTGCCGAACCGCA
CAAACAGGTGCTGGCCGACGCCGAGCAATACCAACCCGAAGCCCTGAAACTGCTCGAGCGCCTGGTCAATATCGACTCCG
GTTCCGGCTATGAACCGGGGCTCAAGCAAGTCAGCGACATCGCCATCGATGAGCTGAAAAAGCTCGGTGCCACCATCGAA
CTGGTGCCCAACACCCCGGAAAAAACCAACCACGTGCTCGCCACGCTCAAAGGCACCGGCAAGGCGAAAATCCTGCTGAT
GGCGCACATGGACACGGTGTTCAAGGAAGGCTCCGCCGCCGAGCGACCGTTCCACATCAAGGACGGCCGCGCCTACGGGC
CGGGCGTGATGGACGACAAGGGCGGGATCGTCGCCGGCATCTACGCGCTGAAAATTCTGAAGAACCTCGATTTCAAGGAC
TACGCGCAAATCACCTTCCTGCTCGATGCCAGCGAAGAAACCGGCTCGGACGTTGCCACCGACCTGATCAAGAAAACCGC
CAAACTCCACGATGTCACCCTCAATCTCGAGCCGGGCCGTCCGGCCGATGGTCTGGTGGTGTGGCGCAAGGGCAGCGCCA
CCGCGCTGGTGGAGGTCAAGGGCAAAGCCGCCCACGCCGGCGTCGCGCCGGAACTGGGGCGCAACGCGGCGATGGAGGCG
GCGCACCAGATCCTGCAACTGGGCAAGCTTGGCGACGAAGCCAAGAAAACCACCATCAACTTCACCGTGCTCAAGGCTGG
CGACCGCACCAACGTGATCCCGGATCAGGCCACGGCCAAGGCTGACGTGCGGGCGGCGGTGCCGGAAGAGTTCGACCGGA
TCGAGAAGGATCTGGCGCGGGTGTCGCAGGACAAGTTGATTCCTGACACGGAGGTGAAGACTTCATTGCAACGCGGTTTG
CCGCCGATGCCGCAGACGGCTGAGTCGGATCGCTTGATGGCGATGGCCCAAGGGATCTACGGTGAGATTGGCCGCAAGTT
GACCGAAGAAGGTAGCGGCGGGGCAGCGGACGCCAGTTTGTCGGCCGGGGTTGGGACGCCGACGCTGGACGGGTTCGGGA
TTGTCGGCGGCAACATTCATACGCCGGAGGAATACGCGGAGGTGGAGAGCGTTGCGCCGCGGATTTATCTGTTGTCGCGG
ATGATTATGGAGTTGGCGAAGGGGCGGTGA

Upstream 100 bases:

>100_bases
ATTCGATTCGGTTTGAACAGATCGGGTGTCGCATCGGGGATAGAGCCTTGGGCGGATGTCAGGTACGGTTCAGTACCCGA
CACGAAACAAGGATTGATTC

Downstream 100 bases:

>100_bases
GCCGGTCGGTGGAGTAGCGGAGTTGGCGGGGTGTCAGGCAGATTTCTCCCTCACCCCAGCCCTCTCCCGGAGGGAGAGGG
AGCTGACCGAGGTGTCTTGC

Product: glutamate carboxypeptidase

Products: NA

Alternate protein names: CPDG2; Folate hydrolase G2; Glutamate carboxypeptidase; Pteroylmonoglutamic acid hydrolase G2; Glucarpidase [H]

Number of amino acids: Translated: 409; Mature: 408

Protein sequence:

>409_residues
MTFKFRRSLLAGLSLTFAASGVFAEPHKQVLADAEQYQPEALKLLERLVNIDSGSGYEPGLKQVSDIAIDELKKLGATIE
LVPNTPEKTNHVLATLKGTGKAKILLMAHMDTVFKEGSAAERPFHIKDGRAYGPGVMDDKGGIVAGIYALKILKNLDFKD
YAQITFLLDASEETGSDVATDLIKKTAKLHDVTLNLEPGRPADGLVVWRKGSATALVEVKGKAAHAGVAPELGRNAAMEA
AHQILQLGKLGDEAKKTTINFTVLKAGDRTNVIPDQATAKADVRAAVPEEFDRIEKDLARVSQDKLIPDTEVKTSLQRGL
PPMPQTAESDRLMAMAQGIYGEIGRKLTEEGSGGAADASLSAGVGTPTLDGFGIVGGNIHTPEEYAEVESVAPRIYLLSR
MIMELAKGR

Sequences:

>Translated_409_residues
MTFKFRRSLLAGLSLTFAASGVFAEPHKQVLADAEQYQPEALKLLERLVNIDSGSGYEPGLKQVSDIAIDELKKLGATIE
LVPNTPEKTNHVLATLKGTGKAKILLMAHMDTVFKEGSAAERPFHIKDGRAYGPGVMDDKGGIVAGIYALKILKNLDFKD
YAQITFLLDASEETGSDVATDLIKKTAKLHDVTLNLEPGRPADGLVVWRKGSATALVEVKGKAAHAGVAPELGRNAAMEA
AHQILQLGKLGDEAKKTTINFTVLKAGDRTNVIPDQATAKADVRAAVPEEFDRIEKDLARVSQDKLIPDTEVKTSLQRGL
PPMPQTAESDRLMAMAQGIYGEIGRKLTEEGSGGAADASLSAGVGTPTLDGFGIVGGNIHTPEEYAEVESVAPRIYLLSR
MIMELAKGR
>Mature_408_residues
TFKFRRSLLAGLSLTFAASGVFAEPHKQVLADAEQYQPEALKLLERLVNIDSGSGYEPGLKQVSDIAIDELKKLGATIEL
VPNTPEKTNHVLATLKGTGKAKILLMAHMDTVFKEGSAAERPFHIKDGRAYGPGVMDDKGGIVAGIYALKILKNLDFKDY
AQITFLLDASEETGSDVATDLIKKTAKLHDVTLNLEPGRPADGLVVWRKGSATALVEVKGKAAHAGVAPELGRNAAMEAA
HQILQLGKLGDEAKKTTINFTVLKAGDRTNVIPDQATAKADVRAAVPEEFDRIEKDLARVSQDKLIPDTEVKTSLQRGLP
PMPQTAESDRLMAMAQGIYGEIGRKLTEEGSGGAADASLSAGVGTPTLDGFGIVGGNIHTPEEYAEVESVAPRIYLLSRM
IMELAKGR

Specific function: Catalyzes the hydrolysis of reduced and non-reduced folates to pteroates and L-glutamate. This enzyme has a broad specificity [H]

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family [H]

Homologues:

Organism=Escherichia coli, GI1790395, Length=399, Percent_Identity=24.5614035087719, Blast_Score=65, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001261
- InterPro:   IPR017150
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.4.17.11 [H]

Molecular weight: Translated: 43748; Mature: 43616

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: PS00758 ARGE_DAPE_CPG2_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFKFRRSLLAGLSLTFAASGVFAEPHKQVLADAEQYQPEALKLLERLVNIDSGSGYEPG
CCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCH
LKQVSDIAIDELKKLGATIELVPNTPEKTNHVLATLKGTGKAKILLMAHMDTVFKEGSAA
HHHHHHHHHHHHHHCCCEEEECCCCCCCCCEEEEEEECCCCEEEEEEEHHHHHHHCCCCC
ERPFHIKDGRAYGPGVMDDKGGIVAGIYALKILKNLDFKDYAQITFLLDASEETGSDVAT
CCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCHHHHH
DLIKKTAKLHDVTLNLEPGRPADGLVVWRKGSATALVEVKGKAAHAGVAPELGRNAAMEA
HHHHHHHHHEEEEEEECCCCCCCCEEEEECCCCEEEEEECCCCCCCCCCHHHCHHHHHHH
AHQILQLGKLGDEAKKTTINFTVLKAGDRTNVIPDQATAKADVRAAVPEEFDRIEKDLAR
HHHHHHHHHCCCHHHHEEEEEEEEECCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHH
VSQDKLIPDTEVKTSLQRGLPPMPQTAESDRLMAMAQGIYGEIGRKLTEEGSGGAADASL
HHHHCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
SAGVGTPTLDGFGIVGGNIHTPEEYAEVESVAPRIYLLSRMIMELAKGR
CCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TFKFRRSLLAGLSLTFAASGVFAEPHKQVLADAEQYQPEALKLLERLVNIDSGSGYEPG
CHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCH
LKQVSDIAIDELKKLGATIELVPNTPEKTNHVLATLKGTGKAKILLMAHMDTVFKEGSAA
HHHHHHHHHHHHHHCCCEEEECCCCCCCCCEEEEEEECCCCEEEEEEEHHHHHHHCCCCC
ERPFHIKDGRAYGPGVMDDKGGIVAGIYALKILKNLDFKDYAQITFLLDASEETGSDVAT
CCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCHHHHH
DLIKKTAKLHDVTLNLEPGRPADGLVVWRKGSATALVEVKGKAAHAGVAPELGRNAAMEA
HHHHHHHHHEEEEEEECCCCCCCCEEEEECCCCEEEEEECCCCCCCCCCHHHCHHHHHHH
AHQILQLGKLGDEAKKTTINFTVLKAGDRTNVIPDQATAKADVRAAVPEEFDRIEKDLAR
HHHHHHHHHCCCHHHHEEEEEEEEECCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHH
VSQDKLIPDTEVKTSLQRGLPPMPQTAESDRLMAMAQGIYGEIGRKLTEEGSGGAADASL
HHHHCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
SAGVGTPTLDGFGIVGGNIHTPEEYAEVESVAPRIYLLSRMIMELAKGR
CCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6396165; 3838935; 9083113 [H]