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The map label for this gene is outD [H]

Identifier: 77458214

GI number: 77458214

Start: 2267635

End: 2269482

Strand: Direct

Name: outD [H]

Synonym: Pfl01_1987

Alternate gene names: 77458214

Gene position: 2267635-2269482 (Clockwise)

Preceding gene: 77458213

Following gene: 77458215

Centisome position: 35.22

GC content: 62.07

Gene sequence:

>1848_bases
ATGAACAGATCCCGTTTGCTGATGAGCCTCGGCCTGTGTGCCGGGCTGGCCGCGTGCAGTTCCGCGCAGGTCGCCAACAA
GGAAGCCGCCGACCTGATCGATCAGGGGCAGTACGAGGCGGGCCTGGCCCGGATCGAGGAAGGCTTGCGGGAAAATCCGC
GCGACACCGAGCTGCACCTGCTGCTCAACAGCGGCCGGGCCAAGGCGATCACCGCGCTGCTGACCTCCGGCGACACCGAC
CGCGCCCGCCGCGACTTCGCCTCGGCCCGCACCGCCTACAGCCGGGTGCTGACCATCGAGCCGAACAACCGCCGCGCCCA
GGATGCCCTGCGCCAGCTCGACTACCTGCGCAGCATGGATGAGAAACTTGAGCTGGCCCGTGGCGACCTGCGCCGGGGCG
ACATCTACGGCGCCGACCGTCAGGTCAAACAGATCCTTGAACTCGACCCGAAAAACGACGGCGCGCTGGAGCTGCAAGGC
AACATCCGTCTGGTGCAGAGCCGCAACGTGATCCAGTACCCGCAACTGCGCACCCGGCTTGATCGCCCGGTGACCCTGGA
ATTTCGCGACGCCAACCTCAAGACCATTTTCGAAGTGCTGTCGCAGGTTGCCGGCCTGAATTTCATCTTCGACAAGGACC
TGCGTCCGGACATGAAAGCCACGATCTTCGTGCGTGACGTGCGCATCGAAGACGCCGTGCAACTGCTGCTGCAACAGAAC
CAGCTGCACCAGAAAGTGGTGAACGAAAACACCTTGCTGATCTTTCCGGACTCGCCGCAGAAGCTCAAGGATTACCAAGA
GCTGGTGATGCGCACCTTCTATCTGACCAGCATCGACGCCAACACCGCGCTGAACATGATCAAGACCATGCTCAAGACCC
GTGACGTGTTCGTCGACGAACGCCTGAACACCCTGACCATGCGCGACACCGAAGACGCCGTGCGCATGGCCGAGAAACTC
CTGCAATCGCAAGACCAGTCCAACCCCGAAGTGGTGCTGGAAGTGGAAGTGATGGAAGTCGCCACCCAGCGCATTCTCGA
CCTCGGCCTGCAATGGCCGAATACCTTCGGCGTGGTCAACAGCGACGGTTCGGCGGTGACCATTCTCGATCAACTGAAAG
GCATCAACTCCAGCCGGATTTCGATCTCGCCGTCGCCGCAAGCCAAGATCAATGCGCAGGACAACGACATCAACACCCTG
GCCAGCCCGGTGATCCGCGTCAGCAACCGCGAACAGGCGCGTATCCATATCGGTCAGCGGGTGCCGATCATCAGCGCCAC
CTCAGTGCCATCCACTCAAGGCCCGGTTATCACCGAAAGCGTCACCTACCTCGACGTCGGTCTCAAGCTTGAAGTGCAAC
CGACCGTGCACTTGAACAACGAAGTAGCAATCAAGATCGCCCTGGAAGTGAGTAACGCCACGCCGCTGGAGCCGACCCGC
CAAGGGACGATTCCGGTGCAGGTCGACACCCGCAACGCCCAGACTTCGCTGCGTCTGCATGACGGCGAAACCCAGATCCT
TGCCGGCCTGATGCGCAACGACCACGGCGCCACCGGCAACAAGATTCCGGGCCTTGGCGACATTCCGGGTCTGGGTCGGT
TGTTCGGCAGCAACAAGGACACTATCGGCAAATCGGAACTGGTGCTCTCGATCACCCCACGGATCGTGCGCAACCTGCCG
TACCAGAGCCCGTCGGACATGGAATTCCCCACCGGCACCGAAACCAGCATGCACATCCAGGCGCCGGATCGCTCGCAAAG
CTACTCGATGCCAGCGCCTGCCGCGCAGCCTCGTGCAGTCGGTGAAGCGGCCGTGGCCACCACCCGTGTAACCGTCGAGA
AGCCTTGA

Upstream 100 bases:

>100_bases
TCGACGACACCTGGAAGATTGCGGCGATTTCCGATGTGGAACTGAGCCTGGTCTACCTGCCTTTGCATTTGTCGCAGACC
TTGTCTGTGGGGAGTACGCA

Downstream 100 bases:

>100_bases
TCATGAACGCCTCCCAACGCGGTTTTACCTTGATCGAAGTCGTGGTGACGCTGGCCCTGATCGGCCTGCTGGCCGGCATG
GCCGCGCCGCTGACCGAGAC

Product: type II and III secretion system protein

Products: NA

Alternate protein names: Pectic enzymes secretion protein outD [H]

Number of amino acids: Translated: 615; Mature: 615

Protein sequence:

>615_residues
MNRSRLLMSLGLCAGLAACSSAQVANKEAADLIDQGQYEAGLARIEEGLRENPRDTELHLLLNSGRAKAITALLTSGDTD
RARRDFASARTAYSRVLTIEPNNRRAQDALRQLDYLRSMDEKLELARGDLRRGDIYGADRQVKQILELDPKNDGALELQG
NIRLVQSRNVIQYPQLRTRLDRPVTLEFRDANLKTIFEVLSQVAGLNFIFDKDLRPDMKATIFVRDVRIEDAVQLLLQQN
QLHQKVVNENTLLIFPDSPQKLKDYQELVMRTFYLTSIDANTALNMIKTMLKTRDVFVDERLNTLTMRDTEDAVRMAEKL
LQSQDQSNPEVVLEVEVMEVATQRILDLGLQWPNTFGVVNSDGSAVTILDQLKGINSSRISISPSPQAKINAQDNDINTL
ASPVIRVSNREQARIHIGQRVPIISATSVPSTQGPVITESVTYLDVGLKLEVQPTVHLNNEVAIKIALEVSNATPLEPTR
QGTIPVQVDTRNAQTSLRLHDGETQILAGLMRNDHGATGNKIPGLGDIPGLGRLFGSNKDTIGKSELVLSITPRIVRNLP
YQSPSDMEFPTGTETSMHIQAPDRSQSYSMPAPAAQPRAVGEAAVATTRVTVEKP

Sequences:

>Translated_615_residues
MNRSRLLMSLGLCAGLAACSSAQVANKEAADLIDQGQYEAGLARIEEGLRENPRDTELHLLLNSGRAKAITALLTSGDTD
RARRDFASARTAYSRVLTIEPNNRRAQDALRQLDYLRSMDEKLELARGDLRRGDIYGADRQVKQILELDPKNDGALELQG
NIRLVQSRNVIQYPQLRTRLDRPVTLEFRDANLKTIFEVLSQVAGLNFIFDKDLRPDMKATIFVRDVRIEDAVQLLLQQN
QLHQKVVNENTLLIFPDSPQKLKDYQELVMRTFYLTSIDANTALNMIKTMLKTRDVFVDERLNTLTMRDTEDAVRMAEKL
LQSQDQSNPEVVLEVEVMEVATQRILDLGLQWPNTFGVVNSDGSAVTILDQLKGINSSRISISPSPQAKINAQDNDINTL
ASPVIRVSNREQARIHIGQRVPIISATSVPSTQGPVITESVTYLDVGLKLEVQPTVHLNNEVAIKIALEVSNATPLEPTR
QGTIPVQVDTRNAQTSLRLHDGETQILAGLMRNDHGATGNKIPGLGDIPGLGRLFGSNKDTIGKSELVLSITPRIVRNLP
YQSPSDMEFPTGTETSMHIQAPDRSQSYSMPAPAAQPRAVGEAAVATTRVTVEKP
>Mature_615_residues
MNRSRLLMSLGLCAGLAACSSAQVANKEAADLIDQGQYEAGLARIEEGLRENPRDTELHLLLNSGRAKAITALLTSGDTD
RARRDFASARTAYSRVLTIEPNNRRAQDALRQLDYLRSMDEKLELARGDLRRGDIYGADRQVKQILELDPKNDGALELQG
NIRLVQSRNVIQYPQLRTRLDRPVTLEFRDANLKTIFEVLSQVAGLNFIFDKDLRPDMKATIFVRDVRIEDAVQLLLQQN
QLHQKVVNENTLLIFPDSPQKLKDYQELVMRTFYLTSIDANTALNMIKTMLKTRDVFVDERLNTLTMRDTEDAVRMAEKL
LQSQDQSNPEVVLEVEVMEVATQRILDLGLQWPNTFGVVNSDGSAVTILDQLKGINSSRISISPSPQAKINAQDNDINTL
ASPVIRVSNREQARIHIGQRVPIISATSVPSTQGPVITESVTYLDVGLKLEVQPTVHLNNEVAIKIALEVSNATPLEPTR
QGTIPVQVDTRNAQTSLRLHDGETQILAGLMRNDHGATGNKIPGLGDIPGLGRLFGSNKDTIGKSELVLSITPRIVRNLP
YQSPSDMEFPTGTETSMHIQAPDRSQSYSMPAPAAQPRAVGEAAVATTRVTVEKP

Specific function: Involved in a general secretion pathway (GSP) for the export of proteins. Required for the translocation of the multiple pectic enzymes [H]

COG id: COG4796

COG function: function code U; Type II secretory pathway, component HofQ

Gene ontology:

Cell location: Cell outer membrane (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP D family [H]

Homologues:

Organism=Escherichia coli, GI1789793, Length=410, Percent_Identity=23.6585365853659, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI87082242, Length=289, Percent_Identity=25.9515570934256, Blast_Score=77, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001775
- InterPro:   IPR005644
- InterPro:   IPR004846
- InterPro:   IPR013356
- InterPro:   IPR004845 [H]

Pfam domain/function: PF00263 Secretin; PF03958 Secretin_N [H]

EC number: NA

Molecular weight: Translated: 68081; Mature: 68081

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS50005 TPR L=RR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRSRLLMSLGLCAGLAACSSAQVANKEAADLIDQGQYEAGLARIEEGLRENPRDTELHL
CCHHHHHHHHHHHHHHHHCCCCHHCCHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEEE
LLNSGRAKAITALLTSGDTDRARRDFASARTAYSRVLTIEPNNRRAQDALRQLDYLRSMD
EEECCCCCEEEEEEECCCCHHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHHHHHHHH
EKLELARGDLRRGDIYGADRQVKQILELDPKNDGALELQGNIRLVQSRNVIQYPQLRTRL
HHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCEEEEECCEEEEECCCCCCCCHHHHHC
DRPVTLEFRDANLKTIFEVLSQVAGLNFIFDKDLRPDMKATIFVRDVRIEDAVQLLLQQN
CCCEEEEEECCCHHHHHHHHHHHCCCEEEECCCCCCCCEEEEEEEECCHHHHHHHHHHHH
QLHQKVVNENTLLIFPDSPQKLKDYQELVMRTFYLTSIDANTALNMIKTMLKTRDVFVDE
HHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHHC
RLNTLTMRDTEDAVRMAEKLLQSQDQSNPEVVLEVEVMEVATQRILDLGLQWPNTFGVVN
CCCEEEECCCHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHCCCCCCCCEEEE
SDGSAVTILDQLKGINSSRISISPSPQAKINAQDNDINTLASPVIRVSNREQARIHIGQR
CCCCEEEEEHHHCCCCCCEEEECCCCCEEECCCCCCHHHHHHHHEEECCCCCEEEEECCC
VPIISATSVPSTQGPVITESVTYLDVGLKLEVQPTVHLNNEVAIKIALEVSNATPLEPTR
CCEEEECCCCCCCCCEEECEEEEEEECEEEEEECEEEECCEEEEEEEEEECCCCCCCCCC
QGTIPVQVDTRNAQTSLRLHDGETQILAGLMRNDHGATGNKIPGLGDIPGLGRLFGSNKD
CCEEEEEEECCCCCEEEEEECCCHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHCCCCC
TIGKSELVLSITPRIVRNLPYQSPSDMEFPTGTETSMHIQAPDRSQSYSMPAPAAQPRAV
CCCCCCEEEEECHHHHHCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCC
GEAAVATTRVTVEKP
CCHHEEEEEEEEECC
>Mature Secondary Structure
MNRSRLLMSLGLCAGLAACSSAQVANKEAADLIDQGQYEAGLARIEEGLRENPRDTELHL
CCHHHHHHHHHHHHHHHHCCCCHHCCHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEEE
LLNSGRAKAITALLTSGDTDRARRDFASARTAYSRVLTIEPNNRRAQDALRQLDYLRSMD
EEECCCCCEEEEEEECCCCHHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHHHHHHHH
EKLELARGDLRRGDIYGADRQVKQILELDPKNDGALELQGNIRLVQSRNVIQYPQLRTRL
HHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCEEEEECCEEEEECCCCCCCCHHHHHC
DRPVTLEFRDANLKTIFEVLSQVAGLNFIFDKDLRPDMKATIFVRDVRIEDAVQLLLQQN
CCCEEEEEECCCHHHHHHHHHHHCCCEEEECCCCCCCCEEEEEEEECCHHHHHHHHHHHH
QLHQKVVNENTLLIFPDSPQKLKDYQELVMRTFYLTSIDANTALNMIKTMLKTRDVFVDE
HHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHHC
RLNTLTMRDTEDAVRMAEKLLQSQDQSNPEVVLEVEVMEVATQRILDLGLQWPNTFGVVN
CCCEEEECCCHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHCCCCCCCCEEEE
SDGSAVTILDQLKGINSSRISISPSPQAKINAQDNDINTLASPVIRVSNREQARIHIGQR
CCCCEEEEEHHHCCCCCCEEEECCCCCEEECCCCCCHHHHHHHHEEECCCCCEEEEECCC
VPIISATSVPSTQGPVITESVTYLDVGLKLEVQPTVHLNNEVAIKIALEVSNATPLEPTR
CCEEEECCCCCCCCCEEECEEEEEEECEEEEEECEEEECCEEEEEEEEEECCCCCCCCCC
QGTIPVQVDTRNAQTSLRLHDGETQILAGLMRNDHGATGNKIPGLGDIPGLGRLFGSNKD
CCEEEEEEECCCCCEEEEEECCCHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHCCCCC
TIGKSELVLSITPRIVRNLPYQSPSDMEFPTGTETSMHIQAPDRSQSYSMPAPAAQPRAV
CCCCCCEEEEECHHHHHCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCC
GEAAVATTRVTVEKP
CCHHEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1429461 [H]