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The map label for this gene is pilC [H]

Identifier: 77458199

GI number: 77458199

Start: 2248394

End: 2249581

Strand: Reverse

Name: pilC [H]

Synonym: Pfl01_1972

Alternate gene names: 77458199

Gene position: 2249581-2248394 (Counterclockwise)

Preceding gene: 77458200

Following gene: 77458198

Centisome position: 34.94

GC content: 62.63

Gene sequence:

>1188_bases
ATGCGATTTCAACTCAAAGCAGTGGGCAAGGCCGGGGTGGTGTCGCTGAGCGTCGAGGCGCCCGGCCAGAGCGAAGCGCG
GCGCATCGCCGAGGATCAGGGCTTGCGTGTGGTCAGCCTGCACGCCGAGAGGCACTGGCGTTCGTTGCGCCTGAGCAAGC
GAGAAACCTTCAACCTGGTGCTGTTCAGCCAGGAACTGACTACCCTGCTCAACGCCGGCCTGCCGCTGATCGATGCGCTG
GAGAGCCTGGCCGAAAAAGAAACCGCCCCCGCCGCCCGCAAAACCTTGAGTGAGCTGGTGCGGCTGCTGTACGAGGGCAA
ATCGTTCTCCCAGGCGCTCGGCCAGTTGTCGGCGGTGTTCCCGCCGTTGTACGTGGCGCTGGTACAGTCCAGCGAGAAGA
CCGGCGCGGTCGGCGATGCGCTGGGCCGTTACGTGAGCTATCGCCAGCGCATGGACGAGGTGCGGCAGAAGATCGTCAGC
GCTTCGATCTACCCGCTGCTGTTGCTGGTGGTCGGCGGTGGCGTGGTGCTGTTTCTGATGGGCTATGTGGTGCCGCGCTT
CAGCCTGGTGTTCGAAGGACTGGGCTCGAACCTGCCGTGGCTGTCGCAGATCCTGATGAGCAGCGGCATGTTTTTGCATG
CGCATCAGGGTGAGTTTTTCGGCCTGTTGGCGACAATCGTCATCGCCCTCGCCGTGCTGCAGAAACAACCGGCGTTTCGC
CGTGGGCTGGATCGGCTGGTGGAGAAACTGCCTGCCGTGCACCAGCGGATTTTCATGTATGAGCTGGCACGGTTTTACCG
GTCACTGGGGATTCTGCTGCAAGGCGGAATTCCGCTGGTGACCGCCATGGGCATGGTGCGTGGCCTGCTCACCGTGGCCT
CGCGAGCTCGGCTGGACCAGGCCTGCGAGCGGGTGCGCGAGGGGCAATCGTTGTCCACTGCCCTTGAGCTCAACCGGCTG
GTGACGCCCGTGTCCCTGCGCCTGCTGCGCGCTGGCGAGCAGTCCGGCAACCTCGGGCAAATGATGGAACGCAGCGCCGA
TTTCTACGACGAGGAAATCAGCCGCTGGATCGAGTGGTTCGTGAGGTTGTTCGAACCGCTGCTCATGACTTTCATCGGCC
TGTTGATCGGGGTGATCGTAATCCTGATGTACATCCCGATCTTCGAACTGGCTTCGAGTATCCACTGA

Upstream 100 bases:

>100_bases
ACAGCGAGTACGACCTGCTCTCGATGGGCAAGGACGGCCAGCCCGGCGGCGAAGGCGAAAACGCCGAAGTCACCAACTGG
CAATAAGCGGAGCTCACGGC

Downstream 100 bases:

>100_bases
CCTCGGGAACGGGTCGGATCACACGCAACAAGCCACATCCACGTTCCCAATCAAGACAACAAAGACGGAGAACGATATGC
ACATTCAAAAGCTCGTGCTC

Product: type II secretion system protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 395; Mature: 395

Protein sequence:

>395_residues
MRFQLKAVGKAGVVSLSVEAPGQSEARRIAEDQGLRVVSLHAERHWRSLRLSKRETFNLVLFSQELTTLLNAGLPLIDAL
ESLAEKETAPAARKTLSELVRLLYEGKSFSQALGQLSAVFPPLYVALVQSSEKTGAVGDALGRYVSYRQRMDEVRQKIVS
ASIYPLLLLVVGGGVVLFLMGYVVPRFSLVFEGLGSNLPWLSQILMSSGMFLHAHQGEFFGLLATIVIALAVLQKQPAFR
RGLDRLVEKLPAVHQRIFMYELARFYRSLGILLQGGIPLVTAMGMVRGLLTVASRARLDQACERVREGQSLSTALELNRL
VTPVSLRLLRAGEQSGNLGQMMERSADFYDEEISRWIEWFVRLFEPLLMTFIGLLIGVIVILMYIPIFELASSIH

Sequences:

>Translated_395_residues
MRFQLKAVGKAGVVSLSVEAPGQSEARRIAEDQGLRVVSLHAERHWRSLRLSKRETFNLVLFSQELTTLLNAGLPLIDAL
ESLAEKETAPAARKTLSELVRLLYEGKSFSQALGQLSAVFPPLYVALVQSSEKTGAVGDALGRYVSYRQRMDEVRQKIVS
ASIYPLLLLVVGGGVVLFLMGYVVPRFSLVFEGLGSNLPWLSQILMSSGMFLHAHQGEFFGLLATIVIALAVLQKQPAFR
RGLDRLVEKLPAVHQRIFMYELARFYRSLGILLQGGIPLVTAMGMVRGLLTVASRARLDQACERVREGQSLSTALELNRL
VTPVSLRLLRAGEQSGNLGQMMERSADFYDEEISRWIEWFVRLFEPLLMTFIGLLIGVIVILMYIPIFELASSIH
>Mature_395_residues
MRFQLKAVGKAGVVSLSVEAPGQSEARRIAEDQGLRVVSLHAERHWRSLRLSKRETFNLVLFSQELTTLLNAGLPLIDAL
ESLAEKETAPAARKTLSELVRLLYEGKSFSQALGQLSAVFPPLYVALVQSSEKTGAVGDALGRYVSYRQRMDEVRQKIVS
ASIYPLLLLVVGGGVVLFLMGYVVPRFSLVFEGLGSNLPWLSQILMSSGMFLHAHQGEFFGLLATIVIALAVLQKQPAFR
RGLDRLVEKLPAVHQRIFMYELARFYRSLGILLQGGIPLVTAMGMVRGLLTVASRARLDQACERVREGQSLSTALELNRL
VTPVSLRLLRAGEQSGNLGQMMERSADFYDEEISRWIEWFVRLFEPLLMTFIGLLIGVIVILMYIPIFELASSIH

Specific function: Involved in the translocation of the type IV pilin (pilA) [H]

COG id: COG1459

COG function: function code NU; Type II secretory pathway, component PulF

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP F family [H]

Homologues:

Organism=Escherichia coli, GI1789724, Length=383, Percent_Identity=27.4151436031332, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI1786295, Length=338, Percent_Identity=28.698224852071, Blast_Score=123, Evalue=3e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003004
- InterPro:   IPR018076
- InterPro:   IPR001992 [H]

Pfam domain/function: PF00482 GSPII_F [H]

EC number: NA

Molecular weight: Translated: 43945; Mature: 43945

Theoretical pI: Translated: 9.90; Mature: 9.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFQLKAVGKAGVVSLSVEAPGQSEARRIAEDQGLRVVSLHAERHWRSLRLSKRETFNLV
CCEEEEECCCCCEEEEEECCCCHHHHHHHHHHCCCEEEEEHHHHHHHHHHHCHHHHHHHE
LFSQELTTLLNAGLPLIDALESLAEKETAPAARKTLSELVRLLYEGKSFSQALGQLSAVF
EEHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
PPLYVALVQSSEKTGAVGDALGRYVSYRQRMDEVRQKIVSASIYPLLLLVVGGGVVLFLM
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GYVVPRFSLVFEGLGSNLPWLSQILMSSGMFLHAHQGEFFGLLATIVIALAVLQKQPAFR
HHHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHCCCHHH
RGLDRLVEKLPAVHQRIFMYELARFYRSLGILLQGGIPLVTAMGMVRGLLTVASRARLDQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
ACERVREGQSLSTALELNRLVTPVSLRLLRAGEQSGNLGQMMERSADFYDEEISRWIEWF
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
VRLFEPLLMTFIGLLIGVIVILMYIPIFELASSIH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRFQLKAVGKAGVVSLSVEAPGQSEARRIAEDQGLRVVSLHAERHWRSLRLSKRETFNLV
CCEEEEECCCCCEEEEEECCCCHHHHHHHHHHCCCEEEEEHHHHHHHHHHHCHHHHHHHE
LFSQELTTLLNAGLPLIDALESLAEKETAPAARKTLSELVRLLYEGKSFSQALGQLSAVF
EEHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
PPLYVALVQSSEKTGAVGDALGRYVSYRQRMDEVRQKIVSASIYPLLLLVVGGGVVLFLM
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GYVVPRFSLVFEGLGSNLPWLSQILMSSGMFLHAHQGEFFGLLATIVIALAVLQKQPAFR
HHHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHCCCHHH
RGLDRLVEKLPAVHQRIFMYELARFYRSLGILLQGGIPLVTAMGMVRGLLTVASRARLDQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
ACERVREGQSLSTALELNRLVTPVSLRLLRAGEQSGNLGQMMERSADFYDEEISRWIEWF
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
VRLFEPLLMTFIGLLIGVIVILMYIPIFELASSIH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1971619; 10984043 [H]