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The map label for this gene is purR [H]

Identifier: 77458149

GI number: 77458149

Start: 2194615

End: 2195634

Strand: Direct

Name: purR [H]

Synonym: Pfl01_1922

Alternate gene names: 77458149

Gene position: 2194615-2195634 (Clockwise)

Preceding gene: 77458148

Following gene: 77458150

Centisome position: 34.09

GC content: 60.98

Gene sequence:

>1020_bases
ATGGCAACGATCAAGGATGTGGCGGCACTTGCAGGAATTTCCTACACGACTGTGTCCCACGTAGTAAACAAGACCCGACC
GGTGAGCGAAGAAGTGCGGCTCAAGGTCGAGGCGGCAATCAAGAGTCTCGACTATGTGCCGAGCGCCGTGGCCCGGTCGC
TGAAAGCGAAAACCACCGCCACCATCGGTTTGCTGGTGCCCAACAGCCTCAACCCATACTTCGCCGAACTGGCGCGCGGG
ATCGAGGATTACTGCGAGCGTAACGGTTACTGCGTGATTCTCTGCAACTCCGACGACAACCCGGACAAGCAGCGCAGCTA
CCTGCGGGTCCTGTTGGAAAAACGCATCGACGGCTTGATCGTCGCCTCGGCGGGTGGCGACAGCGGTCTGGCGCAAGGGT
TGGCGGGTGTAAAGACGCCGATGGTGATCGTCGACCGTGGGCTCGAAGGCGTGGATGCCGACCTGGTGCGCATTGATCAC
GAATATGGCGCGTACCTCGCGACGCGGCATCTGCTCGAGCTCGGTCATCGCGATATCGCCACGATCGGTGGTCCGGCCAG
CACCAGTGTTGCGCAAATGCGTCAGGCCGGTTTTGCTCGAGCGCTGAAAGAGGCCGGTGTTGAAGTGGCCCCGTCGCGCA
TGCTGGAAAGCGACTTCACCAGCACTGGCGGTTACAACGCTGCTTCCATCCTGCTTGAACGTAACCCGCCGAGCGCGATT
TTTGCCGGTAACGACATGATCGGCATTGGTGTGCTGCGGGCGGCGGCAGAGCGCAACGTGCGCGTGCCCGCCGAGTTGTC
GGTGATCGGTTTCGACGATATCCAGATGAGTCGTTACGTGTATCCCGCGCTGACGACCGTGGGCCAGTCGATCCTGCAAT
TGGGCGAGATGGCCGCGGAAGTTCTGTTGCGAAGAATTGCCACCCCGGATCTGGCGACGGATCAGCGGATCGTGACCCCG
AGTATTGTCCTGCGCGAATCGACTGCACCGCTGTCCGGCGTGTTCACCGAATACCGCTGA

Upstream 100 bases:

>100_bases
GACCGAGCCGACCAAGCGCATCATCACCGGTGCGGTGATCGTGATCGCCGTGGTGCTCGACACCTATCGCAGTCAACGCG
CAAGCCGACGGGGCTGATAA

Downstream 100 bases:

>100_bases
AACGAATTGAAGAGTAGTGATGTATGTCCGCAAATGTAGTGGTAATAGGCAGCCTGAACATGGACCTGGTCACCCGGGCG
CCGCGCTTGCCCCGGGGCGG

Product: LacI family transcription regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 339; Mature: 338

Protein sequence:

>339_residues
MATIKDVAALAGISYTTVSHVVNKTRPVSEEVRLKVEAAIKSLDYVPSAVARSLKAKTTATIGLLVPNSLNPYFAELARG
IEDYCERNGYCVILCNSDDNPDKQRSYLRVLLEKRIDGLIVASAGGDSGLAQGLAGVKTPMVIVDRGLEGVDADLVRIDH
EYGAYLATRHLLELGHRDIATIGGPASTSVAQMRQAGFARALKEAGVEVAPSRMLESDFTSTGGYNAASILLERNPPSAI
FAGNDMIGIGVLRAAAERNVRVPAELSVIGFDDIQMSRYVYPALTTVGQSILQLGEMAAEVLLRRIATPDLATDQRIVTP
SIVLRESTAPLSGVFTEYR

Sequences:

>Translated_339_residues
MATIKDVAALAGISYTTVSHVVNKTRPVSEEVRLKVEAAIKSLDYVPSAVARSLKAKTTATIGLLVPNSLNPYFAELARG
IEDYCERNGYCVILCNSDDNPDKQRSYLRVLLEKRIDGLIVASAGGDSGLAQGLAGVKTPMVIVDRGLEGVDADLVRIDH
EYGAYLATRHLLELGHRDIATIGGPASTSVAQMRQAGFARALKEAGVEVAPSRMLESDFTSTGGYNAASILLERNPPSAI
FAGNDMIGIGVLRAAAERNVRVPAELSVIGFDDIQMSRYVYPALTTVGQSILQLGEMAAEVLLRRIATPDLATDQRIVTP
SIVLRESTAPLSGVFTEYR
>Mature_338_residues
ATIKDVAALAGISYTTVSHVVNKTRPVSEEVRLKVEAAIKSLDYVPSAVARSLKAKTTATIGLLVPNSLNPYFAELARGI
EDYCERNGYCVILCNSDDNPDKQRSYLRVLLEKRIDGLIVASAGGDSGLAQGLAGVKTPMVIVDRGLEGVDADLVRIDHE
YGAYLATRHLLELGHRDIATIGGPASTSVAQMRQAGFARALKEAGVEVAPSRMLESDFTSTGGYNAASILLERNPPSAIF
AGNDMIGIGVLRAAAERNVRVPAELSVIGFDDIQMSRYVYPALTTVGQSILQLGEMAAEVLLRRIATPDLATDQRIVTPS
IVLRESTAPLSGVFTEYR

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787948, Length=342, Percent_Identity=43.859649122807, Blast_Score=259, Evalue=2e-70,
Organism=Escherichia coli, GI1790194, Length=330, Percent_Identity=40.9090909090909, Blast_Score=252, Evalue=2e-68,
Organism=Escherichia coli, GI1790369, Length=331, Percent_Identity=30.2114803625378, Blast_Score=181, Evalue=5e-47,
Organism=Escherichia coli, GI1789202, Length=331, Percent_Identity=35.9516616314199, Blast_Score=177, Evalue=8e-46,
Organism=Escherichia coli, GI1788474, Length=332, Percent_Identity=34.6385542168675, Blast_Score=164, Evalue=9e-42,
Organism=Escherichia coli, GI1789068, Length=314, Percent_Identity=31.5286624203822, Blast_Score=147, Evalue=7e-37,
Organism=Escherichia coli, GI1786540, Length=329, Percent_Identity=30.0911854103343, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI1787580, Length=313, Percent_Identity=30.6709265175719, Blast_Score=143, Evalue=2e-35,
Organism=Escherichia coli, GI1787906, Length=250, Percent_Identity=31.6, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI1786268, Length=319, Percent_Identity=26.3322884012539, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1790715, Length=311, Percent_Identity=26.3665594855305, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1789456, Length=303, Percent_Identity=29.7029702970297, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI48994940, Length=305, Percent_Identity=27.5409836065574, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1790689, Length=328, Percent_Identity=24.390243902439, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1790192, Length=243, Percent_Identity=27.5720164609054, Blast_Score=66, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36304; Mature: 36173

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATIKDVAALAGISYTTVSHVVNKTRPVSEEVRLKVEAAIKSLDYVPSAVARSLKAKTTA
CCCHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHE
TIGLLVPNSLNPYFAELARGIEDYCERNGYCVILCNSDDNPDKQRSYLRVLLEKRIDGLI
EEEEEECCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCEE
VASAGGDSGLAQGLAGVKTPMVIVDRGLEGVDADLVRIDHEYGAYLATRHLLELGHRDIA
EEECCCCHHHHHHHHHCCCCEEEEECCCCCCCCHHEEECHHHHHHHHHHHHHHHCCCCCE
TIGGPASTSVAQMRQAGFARALKEAGVEVAPSRMLESDFTSTGGYNAASILLERNPPSAI
ECCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCEEEEEEECCCCCEE
FAGNDMIGIGVLRAAAERNVRVPAELSVIGFDDIQMSRYVYPALTTVGQSILQLGEMAAE
EECCCCHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLLRRIATPDLATDQRIVTPSIVLRESTAPLSGVFTEYR
HHHHHHCCCCCCCCCEEECCCEEEECCCCCHHHHHCCCC
>Mature Secondary Structure 
ATIKDVAALAGISYTTVSHVVNKTRPVSEEVRLKVEAAIKSLDYVPSAVARSLKAKTTA
CCHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHE
TIGLLVPNSLNPYFAELARGIEDYCERNGYCVILCNSDDNPDKQRSYLRVLLEKRIDGLI
EEEEEECCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCEE
VASAGGDSGLAQGLAGVKTPMVIVDRGLEGVDADLVRIDHEYGAYLATRHLLELGHRDIA
EEECCCCHHHHHHHHHCCCCEEEEECCCCCCCCHHEEECHHHHHHHHHHHHHHHCCCCCE
TIGGPASTSVAQMRQAGFARALKEAGVEVAPSRMLESDFTSTGGYNAASILLERNPPSAI
ECCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCEEEEEEECCCCCEE
FAGNDMIGIGVLRAAAERNVRVPAELSVIGFDDIQMSRYVYPALTTVGQSILQLGEMAAE
EECCCCHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLLRRIATPDLATDQRIVTPSIVLRESTAPLSGVFTEYR
HHHHHHCCCCCCCCCEEECCCEEEECCCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA