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The map label for this gene is rbsB [H]

Identifier: 77458146

GI number: 77458146

Start: 2191072

End: 2192028

Strand: Direct

Name: rbsB [H]

Synonym: Pfl01_1919

Alternate gene names: 77458146

Gene position: 2191072-2192028 (Clockwise)

Preceding gene: 77458145

Following gene: 77458147

Centisome position: 34.03

GC content: 58.93

Gene sequence:

>957_bases
ATGAAGCTGCCATTCGCTGGACGTCTTCTTGCTGTCGCCATGCTGGCTGCCGTATCCGCCGCACTGCCTGTCTCTTCGGC
TTTCGCCGAGTCCCCTGAAAAACCCAAAGTCGCCCTGGTCATGAAATCCCTGGCCAACGAATTCTTCCTGACCATGGAAG
ACGGCGCCAAGGCCTACCAGAAAGAACATTCCGCCGATTTCGACCTGATCTCCAACGGCATCAAGGACGAAACCGACACG
GCGGGGCAGACGCGCATCGTCGAGCAGATGATTCTGGCGAAGGTCAACGCACTGGTCATCGCACCGTCTGATTCCAAGGC
GATGGTGCCGGTGATCAAGAAAGCCGTCGATGCCGGTATCACTGTGATCAACATCGACAACCAGCTCGATCCGGCTGTGA
TCAAAAGCAAGAACATCAGCGTTCCGTTCGTAGGCCCGGACAATCGCAAAGGCGCGCGTCTGGTGGGCGAGTACCTGGCC
AAACAACTGAAGGCCGGTGACGAAGTGGGCATCATTGAAGGCGTCTCCACGACCACCAACGCCCAGCAGCGTACCGCAGG
CTTCAAGGACGCGATGGAGGCTGCGCAGATCAAGGTTGTCTCCCTGCAGTCCGGCGATTGGGAGATCGACAAGGGCAACA
AGGTGGCCGCTTCGATTCTCAGCGAATATCCGGACGTCAAGGCGCTGCTGGCCGGCAACGACAGCATGGCCGTGGGCGCC
GTTTCCGCCGTGCGTGCTGCCGGCAAGGCTGGGCAGGTGCTGGTTGTTGGTTACGACAACATCAATGCCATCAAACCGAT
GCTGAAGGACGGCCGCGTTCTGGCGACGGCCGACCAGTACGCCGCGAAGCAAGCGGTGTTTGGCATTGAGACTGCGCTGA
AAATCATCAAGGGCGAGAAAGTCGACAGTGGTGCCAATGGCGTGATCGAAACGCCGGTCGAGCTGGTCACCAAGTAA

Upstream 100 bases:

>100_bases
GTTTGCGCGGCCTTCGCGATGGATTGAATTGTCGTGGGGTGCAGCAAGCTTACGCCAGCGCAAGCGTTGCTCACAATAAT
CATAAGATCGGAGTGAACCC

Downstream 100 bases:

>100_bases
GTCCACTGGCGACACAATGGCGCTCGTCCGTCCGGGCGAGCGCATGGAGATTTTTATGTCAGTTTCCGCTCCGAACGCTG
TCCTCTCGGTCAGCGGTATC

Product: periplasmic binding protein/LacI transcriptional regulator

Products: ADP; phosphate; ribose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 318; Mature: 318

Protein sequence:

>318_residues
MKLPFAGRLLAVAMLAAVSAALPVSSAFAESPEKPKVALVMKSLANEFFLTMEDGAKAYQKEHSADFDLISNGIKDETDT
AGQTRIVEQMILAKVNALVIAPSDSKAMVPVIKKAVDAGITVINIDNQLDPAVIKSKNISVPFVGPDNRKGARLVGEYLA
KQLKAGDEVGIIEGVSTTTNAQQRTAGFKDAMEAAQIKVVSLQSGDWEIDKGNKVAASILSEYPDVKALLAGNDSMAVGA
VSAVRAAGKAGQVLVVGYDNINAIKPMLKDGRVLATADQYAAKQAVFGIETALKIIKGEKVDSGANGVIETPVELVTK

Sequences:

>Translated_318_residues
MKLPFAGRLLAVAMLAAVSAALPVSSAFAESPEKPKVALVMKSLANEFFLTMEDGAKAYQKEHSADFDLISNGIKDETDT
AGQTRIVEQMILAKVNALVIAPSDSKAMVPVIKKAVDAGITVINIDNQLDPAVIKSKNISVPFVGPDNRKGARLVGEYLA
KQLKAGDEVGIIEGVSTTTNAQQRTAGFKDAMEAAQIKVVSLQSGDWEIDKGNKVAASILSEYPDVKALLAGNDSMAVGA
VSAVRAAGKAGQVLVVGYDNINAIKPMLKDGRVLATADQYAAKQAVFGIETALKIIKGEKVDSGANGVIETPVELVTK
>Mature_318_residues
MKLPFAGRLLAVAMLAAVSAALPVSSAFAESPEKPKVALVMKSLANEFFLTMEDGAKAYQKEHSADFDLISNGIKDETDT
AGQTRIVEQMILAKVNALVIAPSDSKAMVPVIKKAVDAGITVINIDNQLDPAVIKSKNISVPFVGPDNRKGARLVGEYLA
KQLKAGDEVGIIEGVSTTTNAQQRTAGFKDAMEAAQIKVVSLQSGDWEIDKGNKVAASILSEYPDVKALLAGNDSMAVGA
VSAVRAAGKAGQVLVVGYDNINAIKPMLKDGRVLATADQYAAKQAVFGIETALKIIKGEKVDSGANGVIETPVELVTK

Specific function: Involved in the high-affinity D-ribose membrane transport system and also serves as the primary chemoreceptor for chemotaxis [H]

COG id: COG1879

COG function: function code G; ABC-type sugar transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 2 family [H]

Homologues:

Organism=Escherichia coli, GI1790192, Length=299, Percent_Identity=33.1103678929766, Blast_Score=138, Evalue=4e-34,
Organism=Escherichia coli, GI1790526, Length=283, Percent_Identity=32.1554770318021, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1790674, Length=204, Percent_Identity=30.3921568627451, Blast_Score=78, Evalue=1e-15,

Paralogues:

None

Copy number: 3940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5900 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 1520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001761 [H]

Pfam domain/function: PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 33335; Mature: 33335

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLPFAGRLLAVAMLAAVSAALPVSSAFAESPEKPKVALVMKSLANEFFLTMEDGAKAYQ
CCCCCHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCHHHHHHHHHHHHEEEEECCCHHHHH
KEHSADFDLISNGIKDETDTAGQTRIVEQMILAKVNALVIAPSDSKAMVPVIKKAVDAGI
HHCCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCEEEEECCCCCCHHHHHHHHHHCCE
TVINIDNQLDPAVIKSKNISVPFVGPDNRKGARLVGEYLAKQLKAGDEVGIIEGVSTTTN
EEEEECCCCCCHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCEEEECCCCCCC
AQQRTAGFKDAMEAAQIKVVSLQSGDWEIDKGNKVAASILSEYPDVKALLAGNDSMAVGA
HHHHHCCHHHHHHHHEEEEEEECCCCEEECCCCHHHHHHHHHCCCCEEEEECCCCCHHHH
VSAVRAAGKAGQVLVVGYDNINAIKPMLKDGRVLATADQYAAKQAVFGIETALKIIKGEK
HHHHHHCCCCCCEEEEECCCCHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCC
VDSGANGVIETPVELVTK
CCCCCCCCEECCHHHHCC
>Mature Secondary Structure
MKLPFAGRLLAVAMLAAVSAALPVSSAFAESPEKPKVALVMKSLANEFFLTMEDGAKAYQ
CCCCCHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCHHHHHHHHHHHHEEEEECCCHHHHH
KEHSADFDLISNGIKDETDTAGQTRIVEQMILAKVNALVIAPSDSKAMVPVIKKAVDAGI
HHCCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCEEEEECCCCCCHHHHHHHHHHCCE
TVINIDNQLDPAVIKSKNISVPFVGPDNRKGARLVGEYLAKQLKAGDEVGIIEGVSTTTN
EEEEECCCCCCHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCEEEECCCCCCC
AQQRTAGFKDAMEAAQIKVVSLQSGDWEIDKGNKVAASILSEYPDVKALLAGNDSMAVGA
HHHHHCCHHHHHHHHEEEEEEECCCCEEECCCCHHHHHHHHHCCCCEEEEECCCCCHHHH
VSAVRAAGKAGQVLVVGYDNINAIKPMLKDGRVLATADQYAAKQAVFGIETALKIIKGEK
HHHHHHCCCCCCEEEEECCCCHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCC
VDSGANGVIETPVELVTK
CCCCCCCCEECCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; ribose [Periplasm]; H2O [C]

Specific reaction: ATP + ribose [Periplasm] + H2O = ADP + phosphate + ribose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6313683; 7686882; 9278503; 3011793; 3011794; 8899705; 9298646; 1583688; 7982928; 9641984 [H]