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The map label for this gene is truA [H]

Identifier: 77458123

GI number: 77458123

Start: 2167466

End: 2168290

Strand: Direct

Name: truA [H]

Synonym: Pfl01_1896

Alternate gene names: 77458123

Gene position: 2167466-2168290 (Clockwise)

Preceding gene: 77458122

Following gene: 77458124

Centisome position: 33.66

GC content: 62.3

Gene sequence:

>825_bases
ATGGCACCCGAAGGCTTTTTCCGCGTCGCGCTGGGCGTTGAATACAAGGGCTCGCGCTACAGCGGCTGGCAGCGTCAGTC
CACCGGTGTGCTCACCGTGCAGGAAACCCTGGAAAAAGCCCTGTCGAAGGTCGCCGACTCGCCGATCTCGCTGCAATGCG
CGGGGCGCACCGACGCCGGTGTGCATGCGTGCGGGCAAGTGGTGCATTTCGATACCCAGGTCGACCGCTCGCTGAAAGCC
TGGGTCATGGGCGCCAACATCAATCTGCCCCACGACATCAGCGTCAGTTGGGCGAAGATCATGCCGGCGCATTTCCATGC
GCGATTCAAGGCGATTGCCCGGCGCTATCGCTATGTGATCTACAACGATCAGATCCGCCCGGCGCATCTCAACGAAGAAA
TCACCTGGAATCACCGTCCGCTGGACGTCGAGCGCATGGCCGAGGCCGCGCAGTACCTGATCGGTACCCATGATTTCAGT
GCCTTCCGTGCCGGCCAGTGCCAGGCCAAGTCGCCGATCAAGCAGATGCATCACCTGCGCGTGACCCGTCACGGCAAAAT
GATCGTTCTGGACATCCGCGCCAACGCGTTCCTGCACCACATGGTGCGCAACATCGCCGGCGTGCTGATGACCATCGGTG
CCGGCGAGCGGCCGGTGGAGTGGATGAAAGAAGTGCTGGAGAGCCGTGAGCGGCGTTCCGGCGGGGTCACGGCGCATCCG
TTCGGCCTGTATCTGGTGCAGGTCGAGTACCACGACGAGTTCCCGTTGCCCGAGCGTTTCATCGGGCCACATTTCCTTAC
GGGTTTCGCGGAACTTGACGGCTGA

Upstream 100 bases:

>100_bases
GTTTGGGTCTGGTTTCCCCGTCCCGCCGCCTTATAATGCCCGCCTTTGTAGATCAGCAGGCTGTACCGACTTGGCAAACA
TAGATAACCCGGCCGCCGAA

Downstream 100 bases:

>100_bases
CGCCCTCGAACGCATTTGTTACCATCCGGGACTTTCTCGGATTTGCCTTGGGGTTTTTATCGACATGTCAGCCGTTCGCA
GCAAGATTTGCGGGATTACC

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I [H]

Number of amino acids: Translated: 274; Mature: 273

Protein sequence:

>274_residues
MAPEGFFRVALGVEYKGSRYSGWQRQSTGVLTVQETLEKALSKVADSPISLQCAGRTDAGVHACGQVVHFDTQVDRSLKA
WVMGANINLPHDISVSWAKIMPAHFHARFKAIARRYRYVIYNDQIRPAHLNEEITWNHRPLDVERMAEAAQYLIGTHDFS
AFRAGQCQAKSPIKQMHHLRVTRHGKMIVLDIRANAFLHHMVRNIAGVLMTIGAGERPVEWMKEVLESRERRSGGVTAHP
FGLYLVQVEYHDEFPLPERFIGPHFLTGFAELDG

Sequences:

>Translated_274_residues
MAPEGFFRVALGVEYKGSRYSGWQRQSTGVLTVQETLEKALSKVADSPISLQCAGRTDAGVHACGQVVHFDTQVDRSLKA
WVMGANINLPHDISVSWAKIMPAHFHARFKAIARRYRYVIYNDQIRPAHLNEEITWNHRPLDVERMAEAAQYLIGTHDFS
AFRAGQCQAKSPIKQMHHLRVTRHGKMIVLDIRANAFLHHMVRNIAGVLMTIGAGERPVEWMKEVLESRERRSGGVTAHP
FGLYLVQVEYHDEFPLPERFIGPHFLTGFAELDG
>Mature_273_residues
APEGFFRVALGVEYKGSRYSGWQRQSTGVLTVQETLEKALSKVADSPISLQCAGRTDAGVHACGQVVHFDTQVDRSLKAW
VMGANINLPHDISVSWAKIMPAHFHARFKAIARRYRYVIYNDQIRPAHLNEEITWNHRPLDVERMAEAAQYLIGTHDFSA
FRAGQCQAKSPIKQMHHLRVTRHGKMIVLDIRANAFLHHMVRNIAGVLMTIGAGERPVEWMKEVLESRERRSGGVTAHPF
GLYLVQVEYHDEFPLPERFIGPHFLTGFAELDG

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs [H]

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family [H]

Homologues:

Organism=Homo sapiens, GI23503259, Length=281, Percent_Identity=29.1814946619217, Blast_Score=94, Evalue=1e-19,
Organism=Escherichia coli, GI1788657, Length=260, Percent_Identity=55.3846153846154, Blast_Score=306, Evalue=1e-84,
Organism=Saccharomyces cerevisiae, GI6321112, Length=233, Percent_Identity=28.3261802575107, Blast_Score=76, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094 [H]

Pfam domain/function: PF01416 PseudoU_synth_1 [H]

EC number: =5.4.99.12 [H]

Molecular weight: Translated: 31036; Mature: 30905

Theoretical pI: Translated: 9.09; Mature: 9.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAPEGFFRVALGVEYKGSRYSGWQRQSTGVLTVQETLEKALSKVADSPISLQCAGRTDAG
CCCCCEEEEEEEEEECCCCCCCCCCCCCCEEEHHHHHHHHHHHHCCCCEEEEECCCCCCC
VHACGQVVHFDTQVDRSLKAWVMGANINLPHDISVSWAKIMPAHFHARFKAIARRYRYVI
HHHHHHEEEECHHHCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEE
YNDQIRPAHLNEEITWNHRPLDVERMAEAAQYLIGTHDFSAFRAGQCQAKSPIKQMHHLR
ECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHCCCCCCCCCHHHHHHHHH
VTRHGKMIVLDIRANAFLHHMVRNIAGVLMTIGAGERPVEWMKEVLESRERRSGGVTAHP
EECCCCEEEEEECHHHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHCCCCEECC
FGLYLVQVEYHDEFPLPERFIGPHFLTGFAELDG
EEEEEEEEEECCCCCCCHHHCCHHHHHHHHHCCC
>Mature Secondary Structure 
APEGFFRVALGVEYKGSRYSGWQRQSTGVLTVQETLEKALSKVADSPISLQCAGRTDAG
CCCCEEEEEEEEEECCCCCCCCCCCCCCEEEHHHHHHHHHHHHCCCCEEEEECCCCCCC
VHACGQVVHFDTQVDRSLKAWVMGANINLPHDISVSWAKIMPAHFHARFKAIARRYRYVI
HHHHHHEEEECHHHCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEE
YNDQIRPAHLNEEITWNHRPLDVERMAEAAQYLIGTHDFSAFRAGQCQAKSPIKQMHHLR
ECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHCCCCCCCCCHHHHHHHHH
VTRHGKMIVLDIRANAFLHHMVRNIAGVLMTIGAGERPVEWMKEVLESRERRSGGVTAHP
EECCCCEEEEEECHHHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHCCCCEECC
FGLYLVQVEYHDEFPLPERFIGPHFLTGFAELDG
EEEEEEEEEECCCCCCCHHHCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]