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The map label for this gene is leuB [H]

Identifier: 77458119

GI number: 77458119

Start: 2161069

End: 2162151

Strand: Direct

Name: leuB [H]

Synonym: Pfl01_1892

Alternate gene names: 77458119

Gene position: 2161069-2162151 (Clockwise)

Preceding gene: 77458118

Following gene: 77458120

Centisome position: 33.57

GC content: 62.33

Gene sequence:

>1083_bases
ATGAGCAAGCAGATTCTGATTCTCCCAGGCGACGGTATTGGTCCGGAAATCATGGCCGAAGCGGTCAAGGTGCTGGAGCT
GGCCAACGAAAAGTACAGCCTGGGCTTCGAGCTGAGCCATGACGTGATCGGTGGCGCCGCCATCGACAAGCACGGCGTGC
CGCTGGCCGACGAAACCCTCGATCGTGCCCGCGCTGCCGACGCTGTGCTGCTGGGCGCCGTGGGCGGCCCGAAATGGGAC
ACCATCGAGCGTGACATCCGCCCTGAGCGCGGCCTGCTGAAAATCCGTGCGCAACTGGGCCTGTTCGGCAACCTGCGTCC
GGCGATCCTGTACCCGCAACTGGCTGACGCTTCGAGCCTGAAGCCGGAAATCGCTGCCGGCCTGGATATCCTGATCGTCC
GTGAACTGACCGGCGGCATCTACTTCGGCGCGCCGCGCGGCACCCGTACCCTGGAAAACGGCGAGCGCCAGTCCTACGAC
ACCCTGCCGTACAGTGAGAGTGAAATCCGCCGTATCGCCCGTGTCGGTTTCGACATGGCCCGCGTGCGTGGCAAGAAACT
GTGCTCGGTGGACAAGGCCAACGTGCTGGCGTCCAGCCAGCTGTGGCGTGAAGTGGTCGAGCAGGTCGCCAAGGATTACC
CGGACGTCGAACTGAGCCATATGTACGTCGACAACGCCGCCATGCAACTGGTGCGTGCACCGAAGCAGTTCGACGTGATC
GTCACCGACAACATGTTCGGTGACATCCTGTCCGACGAAGCGTCGATGCTCACCGGTTCCATCGGCATGCTGCCTTCGGC
CTCGCTGGACGCCAACAACAAGGGCATGTACGAGCCTTGCCACGGTTCGGCGCCGGACATCGCGGGCAAAGGCATCGCCA
ACCCGCTGGCGACCATCCTGTCGGTGTCGATGATGCTGCGTTACAGCTTCAATCTGCACGAAGCGGCCGATGCCATCGAA
AAAGCGGTCAGCGTAGTGCTGGATCAAGGCCTGCGCACCGGCGACATCTTTTCGACCGGTTGCACTAAAGTCGGTACGCA
GGAAATGGGTGACGCAGTAGTCGCCGCGCTGCGGAATCTGTAA

Upstream 100 bases:

>100_bases
AGATGTACTGGTGCTCTGGGCCGAAAAGTAAGTTTCGAACGACAAGCATTTTTCCCGGCGCGCCCCTCGATGGCGCACCG
ACTGAAGACACGAGGAAAGC

Downstream 100 bases:

>100_bases
TCTCTCGGGCCCGCTGCGAAATTCAATACAAAGCAGCGGCCCACTTTTCAAGAAGGTGTAGTTGCGATGAAACGTGTAGG
TCTGATCGGTTGGCGCGGCA

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH [H]

Number of amino acids: Translated: 360; Mature: 359

Protein sequence:

>360_residues
MSKQILILPGDGIGPEIMAEAVKVLELANEKYSLGFELSHDVIGGAAIDKHGVPLADETLDRARAADAVLLGAVGGPKWD
TIERDIRPERGLLKIRAQLGLFGNLRPAILYPQLADASSLKPEIAAGLDILIVRELTGGIYFGAPRGTRTLENGERQSYD
TLPYSESEIRRIARVGFDMARVRGKKLCSVDKANVLASSQLWREVVEQVAKDYPDVELSHMYVDNAAMQLVRAPKQFDVI
VTDNMFGDILSDEASMLTGSIGMLPSASLDANNKGMYEPCHGSAPDIAGKGIANPLATILSVSMMLRYSFNLHEAADAIE
KAVSVVLDQGLRTGDIFSTGCTKVGTQEMGDAVVAALRNL

Sequences:

>Translated_360_residues
MSKQILILPGDGIGPEIMAEAVKVLELANEKYSLGFELSHDVIGGAAIDKHGVPLADETLDRARAADAVLLGAVGGPKWD
TIERDIRPERGLLKIRAQLGLFGNLRPAILYPQLADASSLKPEIAAGLDILIVRELTGGIYFGAPRGTRTLENGERQSYD
TLPYSESEIRRIARVGFDMARVRGKKLCSVDKANVLASSQLWREVVEQVAKDYPDVELSHMYVDNAAMQLVRAPKQFDVI
VTDNMFGDILSDEASMLTGSIGMLPSASLDANNKGMYEPCHGSAPDIAGKGIANPLATILSVSMMLRYSFNLHEAADAIE
KAVSVVLDQGLRTGDIFSTGCTKVGTQEMGDAVVAALRNL
>Mature_359_residues
SKQILILPGDGIGPEIMAEAVKVLELANEKYSLGFELSHDVIGGAAIDKHGVPLADETLDRARAADAVLLGAVGGPKWDT
IERDIRPERGLLKIRAQLGLFGNLRPAILYPQLADASSLKPEIAAGLDILIVRELTGGIYFGAPRGTRTLENGERQSYDT
LPYSESEIRRIARVGFDMARVRGKKLCSVDKANVLASSQLWREVVEQVAKDYPDVELSHMYVDNAAMQLVRAPKQFDVIV
TDNMFGDILSDEASMLTGSIGMLPSASLDANNKGMYEPCHGSAPDIAGKGIANPLATILSVSMMLRYSFNLHEAADAIEK
AVSVVLDQGLRTGDIFSTGCTKVGTQEMGDAVVAALRNL

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate [H]

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=331, Percent_Identity=32.02416918429, Blast_Score=145, Evalue=6e-35,
Organism=Homo sapiens, GI28178816, Length=365, Percent_Identity=29.3150684931507, Blast_Score=119, Evalue=4e-27,
Organism=Homo sapiens, GI28178821, Length=327, Percent_Identity=29.6636085626911, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI4758582, Length=354, Percent_Identity=30.225988700565, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI28178838, Length=333, Percent_Identity=30.3303303303303, Blast_Score=112, Evalue=7e-25,
Organism=Homo sapiens, GI28178819, Length=160, Percent_Identity=35.625, Blast_Score=98, Evalue=9e-21,
Organism=Escherichia coli, GI87081683, Length=357, Percent_Identity=55.1820728291317, Blast_Score=388, Evalue=1e-109,
Organism=Escherichia coli, GI1788101, Length=353, Percent_Identity=35.9773371104816, Blast_Score=193, Evalue=1e-50,
Organism=Escherichia coli, GI1787381, Length=397, Percent_Identity=25.4408060453401, Blast_Score=86, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI71986051, Length=353, Percent_Identity=32.5779036827195, Blast_Score=150, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI17505779, Length=369, Percent_Identity=30.0813008130081, Blast_Score=128, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI25144293, Length=374, Percent_Identity=28.6096256684492, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17550882, Length=371, Percent_Identity=28.5714285714286, Blast_Score=116, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6319830, Length=369, Percent_Identity=49.3224932249323, Blast_Score=327, Evalue=2e-90,
Organism=Saccharomyces cerevisiae, GI6322097, Length=368, Percent_Identity=36.6847826086957, Blast_Score=184, Evalue=3e-47,
Organism=Saccharomyces cerevisiae, GI6324291, Length=355, Percent_Identity=30.9859154929577, Blast_Score=126, Evalue=4e-30,
Organism=Saccharomyces cerevisiae, GI6324709, Length=351, Percent_Identity=32.1937321937322, Blast_Score=124, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24643270, Length=341, Percent_Identity=33.1378299120235, Blast_Score=152, Evalue=5e-37,
Organism=Drosophila melanogaster, GI24643268, Length=341, Percent_Identity=33.1378299120235, Blast_Score=152, Evalue=5e-37,
Organism=Drosophila melanogaster, GI24661184, Length=368, Percent_Identity=32.8804347826087, Blast_Score=139, Evalue=4e-33,
Organism=Drosophila melanogaster, GI161078637, Length=346, Percent_Identity=28.9017341040462, Blast_Score=111, Evalue=7e-25,
Organism=Drosophila melanogaster, GI161078635, Length=346, Percent_Identity=28.9017341040462, Blast_Score=111, Evalue=7e-25,
Organism=Drosophila melanogaster, GI161078633, Length=346, Percent_Identity=28.9017341040462, Blast_Score=111, Evalue=7e-25,
Organism=Drosophila melanogaster, GI24650122, Length=346, Percent_Identity=28.9017341040462, Blast_Score=111, Evalue=7e-25,
Organism=Drosophila melanogaster, GI161078639, Length=344, Percent_Identity=29.0697674418605, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI281362242, Length=354, Percent_Identity=28.2485875706215, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI24648872, Length=354, Percent_Identity=28.2485875706215, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI20130355, Length=342, Percent_Identity=23.3918128654971, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004429 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.85 [H]

Molecular weight: Translated: 38723; Mature: 38592

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKQILILPGDGIGPEIMAEAVKVLELANEKYSLGFELSHDVIGGAAIDKHGVPLADETL
CCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCEEECCHHHCCCEECCCCCCCHHHHH
DRARAADAVLLGAVGGPKWDTIERDIRPERGLLKIRAQLGLFGNLRPAILYPQLADASSL
HHHHHHCEEEEECCCCCCCCHHHHCCCCCCCHHHHHHHHCCCCCCCCCEECCCCCCCCCC
KPEIAAGLDILIVRELTGGIYFGAPRGTRTLENGERQSYDTLPYSESEIRRIARVGFDMA
CCHHHCCCCEEEEEECCCCEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
RVRGKKLCSVDKANVLASSQLWREVVEQVAKDYPDVELSHMYVDNAAMQLVRAPKQFDVI
HHCCHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEHHEEHHHHHHHHHHCCCCEEEE
VTDNMFGDILSDEASMLTGSIGMLPSASLDANNKGMYEPCHGSAPDIAGKGIANPLATIL
EECCHHHHHHCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
SVSMMLRYSFNLHEAADAIEKAVSVVLDQGLRTGDIFSTGCTKVGTQEMGDAVVAALRNL
HHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHCCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SKQILILPGDGIGPEIMAEAVKVLELANEKYSLGFELSHDVIGGAAIDKHGVPLADETL
CCCEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCEEECCHHHCCCEECCCCCCCHHHHH
DRARAADAVLLGAVGGPKWDTIERDIRPERGLLKIRAQLGLFGNLRPAILYPQLADASSL
HHHHHHCEEEEECCCCCCCCHHHHCCCCCCCHHHHHHHHCCCCCCCCCEECCCCCCCCCC
KPEIAAGLDILIVRELTGGIYFGAPRGTRTLENGERQSYDTLPYSESEIRRIARVGFDMA
CCHHHCCCCEEEEEECCCCEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
RVRGKKLCSVDKANVLASSQLWREVVEQVAKDYPDVELSHMYVDNAAMQLVRAPKQFDVI
HHCCHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEHHEEHHHHHHHHHHCCCCEEEE
VTDNMFGDILSDEASMLTGSIGMLPSASLDANNKGMYEPCHGSAPDIAGKGIANPLATIL
EECCHHHHHHCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
SVSMMLRYSFNLHEAADAIEKAVSVVLDQGLRTGDIFSTGCTKVGTQEMGDAVVAALRNL
HHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHCCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA