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The map label for this gene is dusC [H]

Identifier: 77458109

GI number: 77458109

Start: 2146841

End: 2147800

Strand: Direct

Name: dusC [H]

Synonym: Pfl01_1882

Alternate gene names: 77458109

Gene position: 2146841-2147800 (Clockwise)

Preceding gene: 77458108

Following gene: 77458110

Centisome position: 33.34

GC content: 64.27

Gene sequence:

>960_bases
ATGCAAATTGCTCTGGCGCCGATGGAAGGGTTGGTCGACGACATCCTGCGCGATGTCCTGACCCGTGTCGGCGGTATTGA
CTGGTGCGTGACCGAGTTCATCCGGGTCAACGATCAGTTGCTGACTCCGGCCTATTTCCACAAGTTCGGCCCTGAACTGC
TCAACGGTGCGCGCACGGCGTCCGGCGTGCCACTGCGCGTGCAGTTGCTGGGTTCCGATCCGGTGTGCCTGGCGGAAAAC
GCGGCGCTGGCCTGCGAGCTGGGCTCCGAAGTGATCGACCTGAACTTCGGTTGCCCGGCCAAGACCGTCAACAAGTCCCG
TGGCGGTGCGGTACTGCTCAAGGAGCCGGAACTGCTCAACCGGATCGTCGAACACGTGCGTCGCGCCGTACCTGCGCACA
TTCCGGTTACCGCCAAGATGCGCCTGGGTTTCGACAGCCCGGACGGCTCGCTGGTGTGCGCCACGGCACTGGCAGAAGGC
GGCGCCGAACACATCGTGGTGCACGCCCGGACCAAGGTCGACGGCTATAAGCCACCGGCCCATTGGGAGTGGATCCCGCG
CGTACAAGATGTGGTCAAGGTGCCGGTATTTGCCAACGGCGACATCTGGAGCGTCGAGGACTGGCGACGCTGCCGGGAAA
TCAGCGGCGTCGAAGACATCATGCTCGGTCGCGGTCTGGTGTCGCGCCCGGACCTGGCCCGTCAGATCGCCGCCGCCCGT
GCCGGCGAAGAAGTCGTCGAGATGACCTGGGCCGAGCTGATGCCACTGATCCAGGACTTCTGGCTGCAAGCCAAAGCGCA
AATGACCGCGCGCCAATCGCCTGGCCGTTTGAAGCAATGGCTGGCCATGCTGACCCGCAATTATCCCGAAGCCGTCGAGC
TTTTCACCGTGCTGCGCCGGGAGACCGAGCCGGATCAGGTCTCGCGTTTGCTGGGTTTGCCGGTCGCCGAAGCCGCCTGA

Upstream 100 bases:

>100_bases
TCATCGAAGGCTATGGCCCGGCCATACAGACGGCGGACATGGGTTTAACCCGCGAAACCCAGTAAACTGCCGCACGTTTT
TCCTTGAGTGTGTGTTTCCC

Downstream 100 bases:

>100_bases
AAAAAATCTTCAAACGATCTCTTGAAAACAAAACGCAGGTCCCTATCTAAGGGTTACGCGATGCCGAATTCGGGTCGCGG
AGACAAAAAAACTTGCTGAT

Product: dihydrouridine synthase, DuS

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 319; Mature: 319

Protein sequence:

>319_residues
MQIALAPMEGLVDDILRDVLTRVGGIDWCVTEFIRVNDQLLTPAYFHKFGPELLNGARTASGVPLRVQLLGSDPVCLAEN
AALACELGSEVIDLNFGCPAKTVNKSRGGAVLLKEPELLNRIVEHVRRAVPAHIPVTAKMRLGFDSPDGSLVCATALAEG
GAEHIVVHARTKVDGYKPPAHWEWIPRVQDVVKVPVFANGDIWSVEDWRRCREISGVEDIMLGRGLVSRPDLARQIAAAR
AGEEVVEMTWAELMPLIQDFWLQAKAQMTARQSPGRLKQWLAMLTRNYPEAVELFTVLRRETEPDQVSRLLGLPVAEAA

Sequences:

>Translated_319_residues
MQIALAPMEGLVDDILRDVLTRVGGIDWCVTEFIRVNDQLLTPAYFHKFGPELLNGARTASGVPLRVQLLGSDPVCLAEN
AALACELGSEVIDLNFGCPAKTVNKSRGGAVLLKEPELLNRIVEHVRRAVPAHIPVTAKMRLGFDSPDGSLVCATALAEG
GAEHIVVHARTKVDGYKPPAHWEWIPRVQDVVKVPVFANGDIWSVEDWRRCREISGVEDIMLGRGLVSRPDLARQIAAAR
AGEEVVEMTWAELMPLIQDFWLQAKAQMTARQSPGRLKQWLAMLTRNYPEAVELFTVLRRETEPDQVSRLLGLPVAEAA
>Mature_319_residues
MQIALAPMEGLVDDILRDVLTRVGGIDWCVTEFIRVNDQLLTPAYFHKFGPELLNGARTASGVPLRVQLLGSDPVCLAEN
AALACELGSEVIDLNFGCPAKTVNKSRGGAVLLKEPELLNRIVEHVRRAVPAHIPVTAKMRLGFDSPDGSLVCATALAEG
GAEHIVVHARTKVDGYKPPAHWEWIPRVQDVVKVPVFANGDIWSVEDWRRCREISGVEDIMLGRGLVSRPDLARQIAAAR
AGEEVVEMTWAELMPLIQDFWLQAKAQMTARQSPGRLKQWLAMLTRNYPEAVELFTVLRRETEPDQVSRLLGLPVAEAA

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusC subfamily [H]

Homologues:

Organism=Homo sapiens, GI40807366, Length=244, Percent_Identity=31.5573770491803, Blast_Score=93, Evalue=3e-19,
Organism=Homo sapiens, GI31742496, Length=168, Percent_Identity=32.7380952380952, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI239788483, Length=233, Percent_Identity=27.4678111587983, Blast_Score=79, Evalue=8e-15,
Organism=Homo sapiens, GI239788462, Length=233, Percent_Identity=27.4678111587983, Blast_Score=76, Evalue=4e-14,
Organism=Escherichia coli, GI1788462, Length=310, Percent_Identity=44.5161290322581, Blast_Score=259, Evalue=2e-70,
Organism=Escherichia coli, GI1789660, Length=281, Percent_Identity=31.3167259786477, Blast_Score=108, Evalue=5e-25,
Organism=Escherichia coli, GI145693211, Length=244, Percent_Identity=29.9180327868852, Blast_Score=77, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI25144369, Length=216, Percent_Identity=32.8703703703704, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17543114, Length=184, Percent_Identity=29.8913043478261, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17507177, Length=155, Percent_Identity=32.9032258064516, Blast_Score=69, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6323560, Length=217, Percent_Identity=30.4147465437788, Blast_Score=86, Evalue=7e-18,
Organism=Saccharomyces cerevisiae, GI6323437, Length=144, Percent_Identity=28.4722222222222, Blast_Score=65, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24580595, Length=229, Percent_Identity=28.3842794759825, Blast_Score=88, Evalue=7e-18,
Organism=Drosophila melanogaster, GI19920448, Length=229, Percent_Identity=28.3842794759825, Blast_Score=88, Evalue=7e-18,
Organism=Drosophila melanogaster, GI24585320, Length=234, Percent_Identity=29.0598290598291, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI19921524, Length=176, Percent_Identity=30.1136363636364, Blast_Score=80, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 35174; Mature: 35174

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIALAPMEGLVDDILRDVLTRVGGIDWCVTEFIRVNDQLLTPAYFHKFGPELLNGARTA
CEEEECHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCHHCCHHHHHHCCHHHHCCCCCC
SGVPLRVQLLGSDPVCLAENAALACELGSEVIDLNFGCPAKTVNKSRGGAVLLKEPELLN
CCCCEEEEEECCCCEEEECCCEEEEECCCCEEEECCCCCHHHCCCCCCCEEEEECHHHHH
RIVEHVRRAVPAHIPVTAKMRLGFDSPDGSLVCATALAEGGAEHIVVHARTKVDGYKPPA
HHHHHHHHHCCCCCCEEEEEEECCCCCCCCEEEEHHHHCCCCCEEEEEEEECCCCCCCCC
HWEWIPRVQDVVKVPVFANGDIWSVEDWRRCREISGVEDIMLGRGLVSRPDLARQIAAAR
CCCHHHHHHHHHCCCEEECCCEECHHHHHHHHHHCCHHHHHHCCCCCCCCHHHHHHHHHH
AGEEVVEMTWAELMPLIQDFWLQAKAQMTARQSPGRLKQWLAMLTRNYPEAVELFTVLRR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHC
ETEPDQVSRLLGLPVAEAA
CCCHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MQIALAPMEGLVDDILRDVLTRVGGIDWCVTEFIRVNDQLLTPAYFHKFGPELLNGARTA
CEEEECHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCHHCCHHHHHHCCHHHHCCCCCC
SGVPLRVQLLGSDPVCLAENAALACELGSEVIDLNFGCPAKTVNKSRGGAVLLKEPELLN
CCCCEEEEEECCCCEEEECCCEEEEECCCCEEEECCCCCHHHCCCCCCCEEEEECHHHHH
RIVEHVRRAVPAHIPVTAKMRLGFDSPDGSLVCATALAEGGAEHIVVHARTKVDGYKPPA
HHHHHHHHHCCCCCCEEEEEEECCCCCCCCEEEEHHHHCCCCCEEEEEEEECCCCCCCCC
HWEWIPRVQDVVKVPVFANGDIWSVEDWRRCREISGVEDIMLGRGLVSRPDLARQIAAAR
CCCHHHHHHHHHCCCEEECCCEECHHHHHHHHHHCCHHHHHHCCCCCCCCHHHHHHHHHH
AGEEVVEMTWAELMPLIQDFWLQAKAQMTARQSPGRLKQWLAMLTRNYPEAVELFTVLRR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHC
ETEPDQVSRLLGLPVAEAA
CCCHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12534463 [H]