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The map label for this gene is emrB [H]

Identifier: 77458103

GI number: 77458103

Start: 2139692

End: 2141242

Strand: Reverse

Name: emrB [H]

Synonym: Pfl01_1876

Alternate gene names: 77458103

Gene position: 2141242-2139692 (Counterclockwise)

Preceding gene: 77458104

Following gene: 77458101

Centisome position: 33.26

GC content: 59.38

Gene sequence:

>1551_bases
ATGGCCGGTGATTCACTGATCCGCCCGGTCGGCGAACCGACCCGGCGGGACTGGATCGCGGTGATGAGCGTCATGCTTGG
CGCCTTCATGGCGGTGCTCGACATCCAGATCACCAACTCGTCGCTCAAGGATATTCAGGGCGCCCTGTCGGCGACCCTGG
AAGAAGGCTCGTGGATTTCGACGTCTTATCTGGTGGCGGAAATCATCATGATTCCGCTGACCGCCTGGCTGGTGCAGTTG
CTGTCGGCACGGCGGCTGGCGGTGTGGGTGTCGCTGGGGTTTCTGATTTCTTCGCTGTTGTGCTCGATGGCCTGGAGTCT
GGAGAGCATGATCGTGTTTCGCGCCATGCAGGGCTTCACCGGCGGCGCGCTGATCCCGCTGGCGTTCACCCTGACACTGA
TCAAACTCCCCGAACACCACCGCGCCAAAGGCATGGCGATGTTTGCCATGACCGCCACCTTCGCCCCATCCATCGGCCCG
ACCCTCGGCGGCTGGCTCACGGAAAACTGGGGCTGGGAGTACATCTTCTATATCAACATCCCGCCGGGGCTGATCATGAT
CGCCGGGCTGATGTACGGGTTGGAGAAGAAAGAAGCGCATTGGGAGCTGCTGAAAAGCACCGATTACACCGGCATTCTCA
CGCTCGGCATCGGCCTCGGTTGCCTGCAGGTTTTCCTTGAGGAAGGCCATCGCAAGGACTGGCTGGAATCGAGCCTGATC
GTGACGCTGGGCAGCATCGCCCTGCTGAGCCTGATCACCTTCGTGATCGTGCAGATTTCCAAGCCCAACCCGCTCATCAA
CCTCGGCATCCTGCGCAACCGCAATTTCGGGCTGTCGAGTATTTCCAGCCTCGGCATGGGCGTCGGGTTGTACGGTTCGA
TCTATCTGCTGCCGCTGTACCTGGCGCAAATCCAGAACTACAACGCCCTGCAGATCGGCGAAGTGATCATGTGGATGGGC
GTGCCGCAGCTGTTTCTGATTCCGCTAGTGCCGAAACTGATGAAATTCGTCTCGCCGAAATGGCTGTGCACAATCGGTTT
CGGGCTGTTCGGGCTGGCGAGTTTTTCATCCGGGGTGCTCAATCCGGATTTCGCCGGGCCGCAGTTCAACCAGATCCAGA
TCATCCGTGCCTTGGGTCAGCCGCTGATCATGGTGACCATTTCGCTGATCGCCACGGCGTACATCCTGCCGCAAGACGCG
GGCTCAGCATCGAGCCTGTTCAACATTTTGCGCAACCTCGGCGGCGCCATCGGCATCGCCCTGCTCGCTACTTTGCTGGA
TGCGCGCACCAAAACCTATTTCGATTATTTGCGTGAAGCGGTGGTGCCGACCAATCCGCAGGTGGCCGAACGCTTGGCGT
CGATGACCGATCGTTTCGGCAGTGACACGGCGGCGTTGGGCAAGTTGAGCGAGATCGTGCATCAGCAGGCGCTGATCATG
GCCTACAACGATGCGTTTCATTTTGTCGGGATTGCGCTGGGGATCAGCATGTTGGCGATTTTGCTGACCAAGAAATTGCC
CGCCGGACTGAAGGCCGGCGAGGCACATTAA

Upstream 100 bases:

>100_bases
CCGGTCAAGCTGACCTTCAAGGCCGATAACCCGCTGCACGGCAAGATCCGTCCGGGCATGTCCGTCACCGCCACCGTGAA
CATCAAAGACGCCCCTGACA

Downstream 100 bases:

>100_bases
TCTTGAGCGGACGCAGAGCGTCCAGGGCTGCATTCCCACGCAGAGCGTGGGAACGATCCGTTAAACGGCCAACAGCCGCT
CACGCAGCTTGGCGATTTCG

Product: EmrB/QacA family drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 516; Mature: 515

Protein sequence:

>516_residues
MAGDSLIRPVGEPTRRDWIAVMSVMLGAFMAVLDIQITNSSLKDIQGALSATLEEGSWISTSYLVAEIIMIPLTAWLVQL
LSARRLAVWVSLGFLISSLLCSMAWSLESMIVFRAMQGFTGGALIPLAFTLTLIKLPEHHRAKGMAMFAMTATFAPSIGP
TLGGWLTENWGWEYIFYINIPPGLIMIAGLMYGLEKKEAHWELLKSTDYTGILTLGIGLGCLQVFLEEGHRKDWLESSLI
VTLGSIALLSLITFVIVQISKPNPLINLGILRNRNFGLSSISSLGMGVGLYGSIYLLPLYLAQIQNYNALQIGEVIMWMG
VPQLFLIPLVPKLMKFVSPKWLCTIGFGLFGLASFSSGVLNPDFAGPQFNQIQIIRALGQPLIMVTISLIATAYILPQDA
GSASSLFNILRNLGGAIGIALLATLLDARTKTYFDYLREAVVPTNPQVAERLASMTDRFGSDTAALGKLSEIVHQQALIM
AYNDAFHFVGIALGISMLAILLTKKLPAGLKAGEAH

Sequences:

>Translated_516_residues
MAGDSLIRPVGEPTRRDWIAVMSVMLGAFMAVLDIQITNSSLKDIQGALSATLEEGSWISTSYLVAEIIMIPLTAWLVQL
LSARRLAVWVSLGFLISSLLCSMAWSLESMIVFRAMQGFTGGALIPLAFTLTLIKLPEHHRAKGMAMFAMTATFAPSIGP
TLGGWLTENWGWEYIFYINIPPGLIMIAGLMYGLEKKEAHWELLKSTDYTGILTLGIGLGCLQVFLEEGHRKDWLESSLI
VTLGSIALLSLITFVIVQISKPNPLINLGILRNRNFGLSSISSLGMGVGLYGSIYLLPLYLAQIQNYNALQIGEVIMWMG
VPQLFLIPLVPKLMKFVSPKWLCTIGFGLFGLASFSSGVLNPDFAGPQFNQIQIIRALGQPLIMVTISLIATAYILPQDA
GSASSLFNILRNLGGAIGIALLATLLDARTKTYFDYLREAVVPTNPQVAERLASMTDRFGSDTAALGKLSEIVHQQALIM
AYNDAFHFVGIALGISMLAILLTKKLPAGLKAGEAH
>Mature_515_residues
AGDSLIRPVGEPTRRDWIAVMSVMLGAFMAVLDIQITNSSLKDIQGALSATLEEGSWISTSYLVAEIIMIPLTAWLVQLL
SARRLAVWVSLGFLISSLLCSMAWSLESMIVFRAMQGFTGGALIPLAFTLTLIKLPEHHRAKGMAMFAMTATFAPSIGPT
LGGWLTENWGWEYIFYINIPPGLIMIAGLMYGLEKKEAHWELLKSTDYTGILTLGIGLGCLQVFLEEGHRKDWLESSLIV
TLGSIALLSLITFVIVQISKPNPLINLGILRNRNFGLSSISSLGMGVGLYGSIYLLPLYLAQIQNYNALQIGEVIMWMGV
PQLFLIPLVPKLMKFVSPKWLCTIGFGLFGLASFSSGVLNPDFAGPQFNQIQIIRALGQPLIMVTISLIATAYILPQDAG
SASSLFNILRNLGGAIGIALLATLLDARTKTYFDYLREAVVPTNPQVAERLASMTDRFGSDTAALGKLSEIVHQQALIMA
YNDAFHFVGIALGISMLAILLTKKLPAGLKAGEAH

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=476, Percent_Identity=30.8823529411765, Blast_Score=228, Evalue=1e-60,
Organism=Escherichia coli, GI1788710, Length=483, Percent_Identity=31.2629399585921, Blast_Score=221, Evalue=9e-59,
Organism=Escherichia coli, GI1790195, Length=299, Percent_Identity=30.7692307692308, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI87081983, Length=378, Percent_Identity=25.3968253968254, Blast_Score=105, Evalue=8e-24,
Organism=Escherichia coli, GI1788392, Length=279, Percent_Identity=26.5232974910394, Blast_Score=75, Evalue=9e-15,
Organism=Saccharomyces cerevisiae, GI6325455, Length=146, Percent_Identity=26.7123287671233, Blast_Score=67, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 56056; Mature: 55925

Theoretical pI: Translated: 7.62; Mature: 7.62

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGDSLIRPVGEPTRRDWIAVMSVMLGAFMAVLDIQITNSSLKDIQGALSATLEEGSWIS
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHCCCCCCH
TSYLVAEIIMIPLTAWLVQLLSARRLAVWVSLGFLISSLLCSMAWSLESMIVFRAMQGFT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GGALIPLAFTLTLIKLPEHHRAKGMAMFAMTATFAPSIGPTLGGWLTENWGWEYIFYINI
CCHHHHHHHHHHHHHCCCCHHHCCEEHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEEEC
PPGLIMIAGLMYGLEKKEAHWELLKSTDYTGILTLGIGLGCLQVFLEEGHRKDWLESSLI
CHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
VTLGSIALLSLITFVIVQISKPNPLINLGILRNRNFGLSSISSLGMGVGLYGSIYLLPLY
HHHHHHHHHHHHHHHHEEECCCCCEEEEEEEECCCCCHHHHHHHCCCHHHHHHHHHHHHH
LAQIQNYNALQIGEVIMWMGVPQLFLIPLVPKLMKFVSPKWLCTIGFGLFGLASFSSGVL
HHHHCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHCCCCC
NPDFAGPQFNQIQIIRALGQPLIMVTISLIATAYILPQDAGSASSLFNILRNLGGAIGIA
CCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
LLATLLDARTKTYFDYLREAVVPTNPQVAERLASMTDRFGSDTAALGKLSEIVHQQALIM
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
AYNDAFHFVGIALGISMLAILLTKKLPAGLKAGEAH
EECHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
AGDSLIRPVGEPTRRDWIAVMSVMLGAFMAVLDIQITNSSLKDIQGALSATLEEGSWIS
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHCCCCCCH
TSYLVAEIIMIPLTAWLVQLLSARRLAVWVSLGFLISSLLCSMAWSLESMIVFRAMQGFT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GGALIPLAFTLTLIKLPEHHRAKGMAMFAMTATFAPSIGPTLGGWLTENWGWEYIFYINI
CCHHHHHHHHHHHHHCCCCHHHCCEEHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEEEC
PPGLIMIAGLMYGLEKKEAHWELLKSTDYTGILTLGIGLGCLQVFLEEGHRKDWLESSLI
CHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
VTLGSIALLSLITFVIVQISKPNPLINLGILRNRNFGLSSISSLGMGVGLYGSIYLLPLY
HHHHHHHHHHHHHHHHEEECCCCCEEEEEEEECCCCCHHHHHHHCCCHHHHHHHHHHHHH
LAQIQNYNALQIGEVIMWMGVPQLFLIPLVPKLMKFVSPKWLCTIGFGLFGLASFSSGVL
HHHHCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHCCCCC
NPDFAGPQFNQIQIIRALGQPLIMVTISLIATAYILPQDAGSASSLFNILRNLGGAIGIA
CCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
LLATLLDARTKTYFDYLREAVVPTNPQVAERLASMTDRFGSDTAALGKLSEIVHQQALIM
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
AYNDAFHFVGIALGISMLAILLTKKLPAGLKAGEAH
EECHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]