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The map label for this gene is xthA [H]

Identifier: 77458095

GI number: 77458095

Start: 2131194

End: 2131988

Strand: Direct

Name: xthA [H]

Synonym: Pfl01_1868

Alternate gene names: 77458095

Gene position: 2131194-2131988 (Clockwise)

Preceding gene: 77458094

Following gene: 77458096

Centisome position: 33.1

GC content: 60.88

Gene sequence:

>795_bases
ATGAAAAAAATGCGCATTGCCACGTTCAACGTCAACGGCCTGCGTGCCCGTCTGCCAAATCTGCTGGAATGGCTCAGGCG
CGAGCAGCCGGACATCGTCTGTCTGCAAGAGCTCAAGATGCTGGACAGCGCGTTTCCCGCTGCTGATCTGGAGGCCGCCG
GCTACGGTGCCATCTGGCACGGTCAGGCGTCTTGGAACGGGGTGGCGATTCTTGCCCGCGATGCGCAACCGCTGGAGAGC
CGGCGCGGTTTGCCCGGCGATCCCGACGATAAGCACAGCCGCTACCTCGAAGCGGCGGTGCATGGAGTGTTGGTGGGGTG
CCTGTATCTGCCCAATGGCAATCCGCAACCTGGGCCGAAGTTCGACTACAAGCTGGCCTGGTTCGAGCGTCTGATCAAAT
ACGCCCGGGATCTGCAAAGCAGCGATCACCCGGTGGTGCTGGCCGGCGATTACAACGTGGTGCCCACCGATCTGGATATC
TACAACACCCGTTCGTGGCTCAAGGATGCGTTGTTGCAACCGGAGAGCCGCGAGTGCTATCAGCGCCTGCTGGATCAGGG
CTGGACGGACTCTTTGCGGCATCTGTATCCCGAAGATCGGCTGTACACGTTCTGGGACTATTTCCGTCAGCACTGGCAGA
AGAACTCCGGGCTGCGGATCGACCATCTGCTGCTCAATCCGACACTGAGCCCTTATCTGCACGAGGCCGGTGTGGATGCC
TGGGTGCGCAACGAACCGCACGCCAGCGACCACGCGCCGACATGGATTCGCATCGGTTCGCGTAAAAGACGCTAG

Upstream 100 bases:

>100_bases
CAATGGCTGCGCAATCGCGTGTAAACGGAGGATCCAACGCAACCGTCGCGGGGGGACCGGTGTCACAGGTTTTAATCGGT
CAATGGCAGGAGCCCGGACG

Downstream 100 bases:

>100_bases
CGGGTGATTGGTTAATTCAATTGTCGATTCGAGGCCCGGATCCCGTATACATGGCGACCATCGGCGGAACGATCCGCGCC
CCCATGCAAAAGGACCCAGC

Product: exodeoxyribonuclease III

Products: NA

Alternate protein names: EXO III; Exonuclease III; AP endonuclease VI [H]

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MKKMRIATFNVNGLRARLPNLLEWLRREQPDIVCLQELKMLDSAFPAADLEAAGYGAIWHGQASWNGVAILARDAQPLES
RRGLPGDPDDKHSRYLEAAVHGVLVGCLYLPNGNPQPGPKFDYKLAWFERLIKYARDLQSSDHPVVLAGDYNVVPTDLDI
YNTRSWLKDALLQPESRECYQRLLDQGWTDSLRHLYPEDRLYTFWDYFRQHWQKNSGLRIDHLLLNPTLSPYLHEAGVDA
WVRNEPHASDHAPTWIRIGSRKRR

Sequences:

>Translated_264_residues
MKKMRIATFNVNGLRARLPNLLEWLRREQPDIVCLQELKMLDSAFPAADLEAAGYGAIWHGQASWNGVAILARDAQPLES
RRGLPGDPDDKHSRYLEAAVHGVLVGCLYLPNGNPQPGPKFDYKLAWFERLIKYARDLQSSDHPVVLAGDYNVVPTDLDI
YNTRSWLKDALLQPESRECYQRLLDQGWTDSLRHLYPEDRLYTFWDYFRQHWQKNSGLRIDHLLLNPTLSPYLHEAGVDA
WVRNEPHASDHAPTWIRIGSRKRR
>Mature_264_residues
MKKMRIATFNVNGLRARLPNLLEWLRREQPDIVCLQELKMLDSAFPAADLEAAGYGAIWHGQASWNGVAILARDAQPLES
RRGLPGDPDDKHSRYLEAAVHGVLVGCLYLPNGNPQPGPKFDYKLAWFERLIKYARDLQSSDHPVVLAGDYNVVPTDLDI
YNTRSWLKDALLQPESRECYQRLLDQGWTDSLRHLYPEDRLYTFWDYFRQHWQKNSGLRIDHLLLNPTLSPYLHEAGVDA
WVRNEPHASDHAPTWIRIGSRKRR

Specific function: Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction. It exhibits 3'-5'-exonuclease, 3'-phosphomonoesterase, 3'-repair diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=260, Percent_Identity=32.6923076923077, Blast_Score=126, Evalue=2e-29,
Organism=Homo sapiens, GI18375503, Length=260, Percent_Identity=32.6923076923077, Blast_Score=126, Evalue=2e-29,
Organism=Homo sapiens, GI18375501, Length=260, Percent_Identity=32.6923076923077, Blast_Score=126, Evalue=2e-29,
Organism=Escherichia coli, GI1788046, Length=265, Percent_Identity=36.6037735849057, Blast_Score=164, Evalue=8e-42,
Organism=Caenorhabditis elegans, GI71989536, Length=264, Percent_Identity=25.7575757575758, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI221330655, Length=259, Percent_Identity=30.5019305019305, Blast_Score=104, Evalue=6e-23,
Organism=Drosophila melanogaster, GI17136678, Length=259, Percent_Identity=30.5019305019305, Blast_Score=103, Evalue=8e-23,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 30534; Mature: 30534

Theoretical pI: Translated: 7.71; Mature: 7.71

Prosite motif: PS00726 AP_NUCLEASE_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKMRIATFNVNGLRARLPNLLEWLRREQPDIVCLQELKMLDSAFPAADLEAAGYGAIWH
CCCEEEEEECCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEE
GQASWNGVAILARDAQPLESRRGLPGDPDDKHSRYLEAAVHGVLVGCLYLPNGNPQPGPK
CCCCCCCEEEEECCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHEECCCCCCCCCCC
FDYKLAWFERLIKYARDLQSSDHPVVLAGDYNVVPTDLDIYNTRSWLKDALLQPESRECY
CCHHHHHHHHHHHHHHHHCCCCCCEEEECCCEECCCCCCHHHHHHHHHHHHCCCCHHHHH
QRLLDQGWTDSLRHLYPEDRLYTFWDYFRQHWQKNSGLRIDHLLLNPTLSPYLHEAGVDA
HHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHCCCH
WVRNEPHASDHAPTWIRIGSRKRR
HHCCCCCCCCCCCCEEEECCCCCC
>Mature Secondary Structure
MKKMRIATFNVNGLRARLPNLLEWLRREQPDIVCLQELKMLDSAFPAADLEAAGYGAIWH
CCCEEEEEECCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEE
GQASWNGVAILARDAQPLESRRGLPGDPDDKHSRYLEAAVHGVLVGCLYLPNGNPQPGPK
CCCCCCCEEEEECCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHEECCCCCCCCCCC
FDYKLAWFERLIKYARDLQSSDHPVVLAGDYNVVPTDLDIYNTRSWLKDALLQPESRECY
CCHHHHHHHHHHHHHHHHCCCCCCEEEECCCEECCCCCCHHHHHHHHHHHHCCCCHHHHH
QRLLDQGWTDSLRHLYPEDRLYTFWDYFRQHWQKNSGLRIDHLLLNPTLSPYLHEAGVDA
HHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHCCCH
WVRNEPHASDHAPTWIRIGSRKRR
HHCCCCCCCCCCCCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3049539; 9097039; 9278503; 8948651; 7885481 [H]