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The map label for this gene is speE [H]

Identifier: 77457959

GI number: 77457959

Start: 1924004

End: 1924879

Strand: Direct

Name: speE [H]

Synonym: Pfl01_1732

Alternate gene names: 77457959

Gene position: 1924004-1924879 (Clockwise)

Preceding gene: 77457958

Following gene: 77457960

Centisome position: 29.88

GC content: 60.5

Gene sequence:

>876_bases
ATGACCGTCACCAAGACCAGCGAATACCTGGAAACCCTCTACGAAGGCTACGGCCAGCGTTTTCGCATGGAAAAACTGCT
GCACGAAGTGCGCACCGAACACCAGCACCTGGTGATTTTCCAGAACCCGCGCATGGGCCGGGTGATGGCGCTGGACGGCG
TGATCCAGACCACCGAAGCCGATGAGTTCATCTACCACGAAATGCTCACCCACGTGCCGATCCTCGCCCACGGCACCGCC
AAGCGCGTGCTGATCATTGGCGGCGGCGACGGCGGCATGCTGCGCGAAGTGACCAAACACGCCAGCGTCGAGCACATCAC
CATGGTCGAGATCGACGGCACCGTGGTCGACATGTGCAAAGAGTTTCTGCCGAACCACTCCAGCGGTGCGTACGACGATC
CACGCCTGAACCTGGTGATCGACGACGGCATGCGTTTCGTCGCCACCACCACTGAAAAATTCGACGTGATCATTTCCGAC
TCCACCGACCCGATCGGTCCGGGCGAAGTGCTGTTCTCGGAAAACTTCTACCAGGCCTGCCACCGCTGCCTGAACGAGGG
CGGCATCCTCGTGACCCAGAACGGCACGCCGTTCATGCAGATCGAAGAAGTGAAAACCACCGCCGGCCGCCTGCGCAGCC
TGTTCCCGGACTGGCACTTCTATCAGGCAGCCGTCCCGACCTACATCGGCGGTTCGATGACCTTTGCCTGGGGCTCGACC
AACCCGGCCTATCGCAAGCTGAGCCGCGAAGTTCTGCAACAGCGCTTCATCGGTAGCGGCATCGTCACCCGCTACTACAA
CCCGGAAATCCATATCGGCGCCTTCGCCTTGCCGCAATACGTGTTGCAAGCGGTGAACAAGCCAAGCAACGACTGA

Upstream 100 bases:

>100_bases
TGAGGTTCGACCTGTCATCTTCGGCAACGTAGAATGCGCGCCTTTCCCGGGCAATCGAACCTGACGATTGGCGCCCGCGC
ATTCATCGGAGCACTTCACC

Downstream 100 bases:

>100_bases
TCATTCGCGGGTCAGAAAAAGCCGAGGCCGGTGTGCGAAAACACGCCGGCCTCGGCTTTTTTCATGTTGCATCGGTGTAA
ACCTTTTGAACGATCAATCC

Product: spermidine synthase

Products: NA

Alternate protein names: Putrescine aminopropyltransferase; PAPT; SPDSY [H]

Number of amino acids: Translated: 291; Mature: 290

Protein sequence:

>291_residues
MTVTKTSEYLETLYEGYGQRFRMEKLLHEVRTEHQHLVIFQNPRMGRVMALDGVIQTTEADEFIYHEMLTHVPILAHGTA
KRVLIIGGGDGGMLREVTKHASVEHITMVEIDGTVVDMCKEFLPNHSSGAYDDPRLNLVIDDGMRFVATTTEKFDVIISD
STDPIGPGEVLFSENFYQACHRCLNEGGILVTQNGTPFMQIEEVKTTAGRLRSLFPDWHFYQAAVPTYIGGSMTFAWGST
NPAYRKLSREVLQQRFIGSGIVTRYYNPEIHIGAFALPQYVLQAVNKPSND

Sequences:

>Translated_291_residues
MTVTKTSEYLETLYEGYGQRFRMEKLLHEVRTEHQHLVIFQNPRMGRVMALDGVIQTTEADEFIYHEMLTHVPILAHGTA
KRVLIIGGGDGGMLREVTKHASVEHITMVEIDGTVVDMCKEFLPNHSSGAYDDPRLNLVIDDGMRFVATTTEKFDVIISD
STDPIGPGEVLFSENFYQACHRCLNEGGILVTQNGTPFMQIEEVKTTAGRLRSLFPDWHFYQAAVPTYIGGSMTFAWGST
NPAYRKLSREVLQQRFIGSGIVTRYYNPEIHIGAFALPQYVLQAVNKPSND
>Mature_290_residues
TVTKTSEYLETLYEGYGQRFRMEKLLHEVRTEHQHLVIFQNPRMGRVMALDGVIQTTEADEFIYHEMLTHVPILAHGTAK
RVLIIGGGDGGMLREVTKHASVEHITMVEIDGTVVDMCKEFLPNHSSGAYDDPRLNLVIDDGMRFVATTTEKFDVIISDS
TDPIGPGEVLFSENFYQACHRCLNEGGILVTQNGTPFMQIEEVKTTAGRLRSLFPDWHFYQAAVPTYIGGSMTFAWGSTN
PAYRKLSREVLQQRFIGSGIVTRYYNPEIHIGAFALPQYVLQAVNKPSND

Specific function: Catalyzes the production of spermidine from putrescine and decarboxylated S-adenosylmethionine (dcSAM), which serves as an aminopropyl donor [H]

COG id: COG0421

COG function: function code E; Spermidine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the spermidine/spermine synthase family [H]

Homologues:

Organism=Homo sapiens, GI63253298, Length=280, Percent_Identity=38.9285714285714, Blast_Score=220, Evalue=1e-57,
Organism=Escherichia coli, GI1786312, Length=288, Percent_Identity=60.0694444444444, Blast_Score=374, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI25154106, Length=277, Percent_Identity=41.5162454873646, Blast_Score=198, Evalue=3e-51,
Organism=Caenorhabditis elegans, GI212645616, Length=322, Percent_Identity=36.0248447204969, Blast_Score=185, Evalue=2e-47,
Organism=Saccharomyces cerevisiae, GI6323175, Length=287, Percent_Identity=41.1149825783972, Blast_Score=226, Evalue=4e-60,
Organism=Saccharomyces cerevisiae, GI6325326, Length=281, Percent_Identity=35.9430604982206, Blast_Score=200, Evalue=2e-52,
Organism=Drosophila melanogaster, GI24645443, Length=275, Percent_Identity=37.4545454545455, Blast_Score=197, Evalue=7e-51,
Organism=Drosophila melanogaster, GI21355941, Length=214, Percent_Identity=38.785046728972, Blast_Score=157, Evalue=7e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001045 [H]

Pfam domain/function: PF01564 Spermine_synth [H]

EC number: =2.5.1.16 [H]

Molecular weight: Translated: 32766; Mature: 32635

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: PS01330 SPERMIDINE_SYNTHASE_1 ; PS51006 SPERMIDINE_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVTKTSEYLETLYEGYGQRFRMEKLLHEVRTEHQHLVIFQNPRMGRVMALDGVIQTTEA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCEEEEECCEEECCCH
DEFIYHEMLTHVPILAHGTAKRVLIIGGGDGGMLREVTKHASVEHITMVEIDGTVVDMCK
HHHHHHHHHHHCCEEEECCCCEEEEEECCCCHHHHHHHHHCCCCEEEEEEECCHHHHHHH
EFLPNHSSGAYDDPRLNLVIDDGMRFVATTTEKFDVIISDSTDPIGPGEVLFSENFYQAC
HHCCCCCCCCCCCCEEEEEEECCCEEEEECCCEEEEEEECCCCCCCCCCEEECCHHHHHH
HRCLNEGGILVTQNGTPFMQIEEVKTTAGRLRSLFPDWHFYQAAVPTYIGGSMTFAWGST
HHHHCCCCEEEECCCCCCEEHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEEEECCC
NPAYRKLSREVLQQRFIGSGIVTRYYNPEIHIGAFALPQYVLQAVNKPSND
CHHHHHHHHHHHHHHHCCCCCEEEEECCCEEEEHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TVTKTSEYLETLYEGYGQRFRMEKLLHEVRTEHQHLVIFQNPRMGRVMALDGVIQTTEA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCEEEEECCEEECCCH
DEFIYHEMLTHVPILAHGTAKRVLIIGGGDGGMLREVTKHASVEHITMVEIDGTVVDMCK
HHHHHHHHHHHCCEEEECCCCEEEEEECCCCHHHHHHHHHCCCCEEEEEEECCHHHHHHH
EFLPNHSSGAYDDPRLNLVIDDGMRFVATTTEKFDVIISDSTDPIGPGEVLFSENFYQAC
HHCCCCCCCCCCCCEEEEEEECCCEEEEECCCEEEEEEECCCCCCCCCCEEECCHHHHHH
HRCLNEGGILVTQNGTPFMQIEEVKTTAGRLRSLFPDWHFYQAAVPTYIGGSMTFAWGST
HHHHCCCCEEEECCCCCCEEHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEEEECCC
NPAYRKLSREVLQQRFIGSGIVTRYYNPEIHIGAFALPQYVLQAVNKPSND
CHHHHHHHHHHHHHHHCCCCCEEEEECCCEEEEHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12928499 [H]