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The map label for this gene is cobB [H]

Identifier: 77457869

GI number: 77457869

Start: 1833042

End: 1834337

Strand: Direct

Name: cobB [H]

Synonym: Pfl01_1642

Alternate gene names: 77457869

Gene position: 1833042-1834337 (Clockwise)

Preceding gene: 77457868

Following gene: 77457870

Centisome position: 28.47

GC content: 64.43

Gene sequence:

>1296_bases
TTGAATCAACCACGTCACTGCCCGGCGGTTCTGATCGCCGCGCCGGCTTCCGGTCAGGGCAAGACCACCGTCACCGCCGC
GCTGGCCCGTCTGCATCGCAATCAGGGGCGCAAGGTCCGCGTGTTCAAATGCGGCCCGGACTTTCTCGACCCGATGATCC
TCGAGCGCGCCAGCGGTGCGCCGGTCTATCAACTGGACATGTGGATGGTCGGCGAGCAGGAAAGTCGTCGCCTGTTGTGG
GAAGCCGCCGCCGAAGCCGATCTGATCCTGATCGAAGGCGTGATGGGTTTGTTTGACGGTACGCCGTCGAGCGCCGATCT
GGCGCGGCATTTCGGCGTGCCGGTGCTCGGCGTGATCGACGGCACGGCCATGGCGCAGACCTTCGGCGCTTTGGCTCTGG
GTTTGGCGCGCTATCAGCCGGACCTGCCGTTCGCCGGCGTGCTGGCCAACCGTGTCGGCACGGTGCGCCATGCGCAATTG
CTCGAAGGCAGCCTCACCGAAGGTTTGCGCTGGTATGGCGCGTTGTCCCGGGAAACCGGGATCGAACTGCCGAGCCGCCA
TCTCGGTCTGGTGCAGGCCAGCGAACTCAATGACCTCGATGTGCGCCTCGACGCCGCCGCCGAAGCGCTGGCCGGCACTT
GCGAGGTAGCGCTGCCACCGGCCGTGGAATTCGCCGCCCCAGAAATCGTTGAGACCGAAAAGCTGCTCGAAGGCGTGCGA
ATTGCTGTCGCCCGCGACGAAGCCTTCGCTTTCACCTATGGCGCCAGTCTTGATCTGTTGCGGGCGATGGGCGCCGAGTT
GAGTTTCTTCTCGCCGATCCGCGATATGCAATTGCCCGACGCCGACAGTTTGTACCTGCCCGGCGGCTATCCGGAATTGC
ACCACGTTGCCTTGTCGCAGAACGCCTACATGCTCAAAGCGATCCGCGCGCACCATGCGGCTGGCAAACCATTGCTTGCC
GAATGCGGCGGCATGCTTTATCTGCTCGACTCGCTGACCGATGTCGAAGGCACCCGCGCGGAGCTGGTCGGCCTGTTGAG
TGGCGACGCCGTGATGCAAAAGCGCCTCGCCGCGCTGGCGCTGCAAGAGGTCGACCTGCCGGAAGGCCTGTTGCGCGGCC
ACACCTATCACCATTCCCTGACCACCACCGAACTGACGCCGATTGCCCGTGGCCACAGCCCCAATGGGGGGCGCGGCGCA
GAAGCGGTGTACCGGCAAGGGCGGATGACCGCCTCGTACGTACATTTTTACTTCCCGTCGAATCCGTCGGCGATTGCCGC
ACTGTTGGTGCCATGA

Upstream 100 bases:

>100_bases
CCAAGCCGGAAATGATCGAGATGGGCGACACCGTCACCGAAATGGGCATGATCAAGCACGCCTTCCAAGCCGGTATCAAA
GCGCAGAAAGGCGTCGAACT

Downstream 100 bases:

>100_bases
CCGACAACGCCTTCACTGACGCCGAGCGCGAAGCGGTCTACCGCGCCATCGCCGAACGCCGCGACATGCGCCACTTCAGC
GGCGGTACGGTCGAGCCTGA

Product: cobyrinic acid a,c-diamide synthase

Products: ADP; phosphate; Hydrogenobyrinic acid a,c diamide; L-glutamate

Alternate protein names: NA

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MNQPRHCPAVLIAAPASGQGKTTVTAALARLHRNQGRKVRVFKCGPDFLDPMILERASGAPVYQLDMWMVGEQESRRLLW
EAAAEADLILIEGVMGLFDGTPSSADLARHFGVPVLGVIDGTAMAQTFGALALGLARYQPDLPFAGVLANRVGTVRHAQL
LEGSLTEGLRWYGALSRETGIELPSRHLGLVQASELNDLDVRLDAAAEALAGTCEVALPPAVEFAAPEIVETEKLLEGVR
IAVARDEAFAFTYGASLDLLRAMGAELSFFSPIRDMQLPDADSLYLPGGYPELHHVALSQNAYMLKAIRAHHAAGKPLLA
ECGGMLYLLDSLTDVEGTRAELVGLLSGDAVMQKRLAALALQEVDLPEGLLRGHTYHHSLTTTELTPIARGHSPNGGRGA
EAVYRQGRMTASYVHFYFPSNPSAIAALLVP

Sequences:

>Translated_431_residues
MNQPRHCPAVLIAAPASGQGKTTVTAALARLHRNQGRKVRVFKCGPDFLDPMILERASGAPVYQLDMWMVGEQESRRLLW
EAAAEADLILIEGVMGLFDGTPSSADLARHFGVPVLGVIDGTAMAQTFGALALGLARYQPDLPFAGVLANRVGTVRHAQL
LEGSLTEGLRWYGALSRETGIELPSRHLGLVQASELNDLDVRLDAAAEALAGTCEVALPPAVEFAAPEIVETEKLLEGVR
IAVARDEAFAFTYGASLDLLRAMGAELSFFSPIRDMQLPDADSLYLPGGYPELHHVALSQNAYMLKAIRAHHAAGKPLLA
ECGGMLYLLDSLTDVEGTRAELVGLLSGDAVMQKRLAALALQEVDLPEGLLRGHTYHHSLTTTELTPIARGHSPNGGRGA
EAVYRQGRMTASYVHFYFPSNPSAIAALLVP
>Mature_431_residues
MNQPRHCPAVLIAAPASGQGKTTVTAALARLHRNQGRKVRVFKCGPDFLDPMILERASGAPVYQLDMWMVGEQESRRLLW
EAAAEADLILIEGVMGLFDGTPSSADLARHFGVPVLGVIDGTAMAQTFGALALGLARYQPDLPFAGVLANRVGTVRHAQL
LEGSLTEGLRWYGALSRETGIELPSRHLGLVQASELNDLDVRLDAAAEALAGTCEVALPPAVEFAAPEIVETEKLLEGVR
IAVARDEAFAFTYGASLDLLRAMGAELSFFSPIRDMQLPDADSLYLPGGYPELHHVALSQNAYMLKAIRAHHAAGKPLLA
ECGGMLYLLDSLTDVEGTRAELVGLLSGDAVMQKRLAALALQEVDLPEGLLRGHTYHHSLTTTELTPIARGHSPNGGRGA
EAVYRQGRMTASYVHFYFPSNPSAIAALLVP

Specific function: Responsible for the amidation of carboxylic groups at position A and C of either cobyrinic acid or hydrogenobrynic acid. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation [H]

COG id: COG1797

COG function: function code H; Cobyrinic acid a,c-diamide synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GATase cobBQ-type domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002586
- InterPro:   IPR004484
- InterPro:   IPR017929
- InterPro:   IPR011698 [H]

Pfam domain/function: PF01656 CbiA; PF07685 GATase_3 [H]

EC number: 6.3.5.9

Molecular weight: Translated: 46182; Mature: 46182

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQPRHCPAVLIAAPASGQGKTTVTAALARLHRNQGRKVRVFKCGPDFLDPMILERASGA
CCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCHHHCHHHHHHCCCC
PVYQLDMWMVGEQESRRLLWEAAAEADLILIEGVMGLFDGTPSSADLARHFGVPVLGVID
CEEEEEEEEECCHHHHHHHHHHHCCCCEEEEEHHHHHHCCCCCHHHHHHHCCCCEEEEEC
GTAMAQTFGALALGLARYQPDLPFAGVLANRVGTVRHAQLLEGSLTEGLRWYGALSRETG
CHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
IELPSRHLGLVQASELNDLDVRLDAAAEALAGTCEVALPPAVEFAAPEIVETEKLLEGVR
CCCCCHHCCEEEECCCCCCEEEEHHHHHHHCCCCEEECCCCHHHCCCHHHHHHHHHCCCE
IAVARDEAFAFTYGASLDLLRAMGAELSFFSPIRDMQLPDADSLYLPGGYPELHHVALSQ
EEEEECCEEEEEECCHHHHHHHHCCCCCHHCCHHHCCCCCCCCEECCCCCCHHHEEEECC
NAYMLKAIRAHHAAGKPLLAECGGMLYLLDSLTDVEGTRAELVGLLSGDAVMQKRLAALA
CHHHHHHHHHHHCCCCCHHHHCCCCEEEEHHHCCCCCCHHHHEEHHCCHHHHHHHHHHHH
LQEVDLPEGLLRGHTYHHSLTTTELTPIARGHSPNGGRGAEAVYRQGRMTASYVHFYFPS
HHHCCCCHHHHCCCEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCEEEEEEEEECC
NPSAIAALLVP
CCCEEEEEECC
>Mature Secondary Structure
MNQPRHCPAVLIAAPASGQGKTTVTAALARLHRNQGRKVRVFKCGPDFLDPMILERASGA
CCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCHHHCHHHHHHCCCC
PVYQLDMWMVGEQESRRLLWEAAAEADLILIEGVMGLFDGTPSSADLARHFGVPVLGVID
CEEEEEEEEECCHHHHHHHHHHHCCCCEEEEEHHHHHHCCCCCHHHHHHHCCCCEEEEEC
GTAMAQTFGALALGLARYQPDLPFAGVLANRVGTVRHAQLLEGSLTEGLRWYGALSRETG
CHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
IELPSRHLGLVQASELNDLDVRLDAAAEALAGTCEVALPPAVEFAAPEIVETEKLLEGVR
CCCCCHHCCEEEECCCCCCEEEEHHHHHHHCCCCEEECCCCHHHCCCHHHHHHHHHCCCE
IAVARDEAFAFTYGASLDLLRAMGAELSFFSPIRDMQLPDADSLYLPGGYPELHHVALSQ
EEEEECCEEEEEECCHHHHHHHHCCCCCHHCCHHHCCCCCCCCEECCCCCCHHHEEEECC
NAYMLKAIRAHHAAGKPLLAECGGMLYLLDSLTDVEGTRAELVGLLSGDAVMQKRLAALA
CHHHHHHHHHHHCCCCCHHHHCCCCEEEEHHHCCCCCCHHHHEEHHCCHHHHHHHHHHHH
LQEVDLPEGLLRGHTYHHSLTTTELTPIARGHSPNGGRGAEAVYRQGRMTASYVHFYFPS
HHHCCCCHHHHCCCEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCEEEEEEEEECC
NPSAIAALLVP
CCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; hydrogenobyrinic acid; L-glutamine; H2O

Specific reaction: 2 ATP + hydrogenobyrinic acid + 2 L-glutamine + 2 H2O = 2 ADP + 2 phosphate + hydrogenobyrinic acid a,c-diamide + 2 L-glutamate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA