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The map label for this gene is afsQ1 [H]

Identifier: 77457769

GI number: 77457769

Start: 1730210

End: 1731913

Strand: Direct

Name: afsQ1 [H]

Synonym: Pfl01_1542

Alternate gene names: 77457769

Gene position: 1730210-1731913 (Clockwise)

Preceding gene: 77457768

Following gene: 77457770

Centisome position: 26.87

GC content: 61.91

Gene sequence:

>1704_bases
ATGCAGCCGCAAGAGCCGCTGACGATCCTGATCGCCGAAGACAGCGCCGCTGACCGGCTGTTGTTGTCGACCATCGTCCG
TCGTCAGGGGCACGAAGTGCTGACGGCCACCCACGGAGCGGAGGCGGTCGAGGCATTTATCCGGCAAAAGCCGCATCTGG
TGTTGATGGACGCCATGATGCCGGTGATGGACGGGTTCGAAGCCGCACGGCAGATCAAGAAGCTGGCGGGTGAGAACCTG
GTGCCGATCATCTTCCTCACCTCGTTGACGGAAAGCGAAGCCCTGGCTCGTTGTCTTGAGGCCGGCGGTGACGACTTTCT
GGCGAAGCCCTACAACCAGGTGATTCTCGCCGCCAAAATCAAGGCGATGGATCGCTTGCGGCGTTTGCAGGCCACGGTTC
TTCAACAGCGCGACCTGATCGCCAAACACCACGACTATCTGCTCAACGAACAGCGGGTCGCCAAGGCCGTGTTCGACAAG
GTGGCGCATTCCGGCTGCCTGAGCGCGCCGAATATCCGTTACCTGCAATCGCCCTATGCGCTGTTCAATGGCGATCTGTT
GTTGGCTGCGTACACTCCGGCCGGCGACATGCACGTATTGCTCGGTGACTTCACCGGTCATGGATTGCCCGCGGCGGTCG
GTGCCATGCCGCTGGCCGAGGTGTTCTACGGCATGACTGCCAAGGGTTACGGCCTGGCCGAAACCCTGCGGGAAATGAAT
GCCAAGCTCAAGCGCATCCTCCCGGTGGACATGTTCTGCTGTGCGACGCTGTTGTGCCTGAGTTTTCAGCGGCGCTCGGT
GGAAGTCTGGAACGGCGGCATGCCCGACGGTTACCTGCATTGCCTCGCCACCGGGGAGCGCAAGCCACTGGCGGCGCGCC
ACTTGCCGCTGGGTGTGCTCAGCCCGCAGACCTTCGATGACCGCACCGAGGTTCACTCGATGGCGGTGGGCGATCGGGTG
TTTCTGCTCTCCGACGGGGTCATCGATACCTGTGATGCCAACGACCAGTTGTTCGGTGTCGAGCGCTTGCACAAGGTGTT
CGCCGCCAATCGCCAGCCGGATCGATTGTTCGAGGACATCGAGCAGGCGTTGCAGGATTTTCGCGGTGAGGCCCGGGACG
ATGTGAGCATGGTCGAGGTCAGTCTGCTGGAAGCCGAGCAGGTCAACCCGCCGGCGCCGGTGTATTCGGACAGTGGTCAG
TCCTGTCCGCTGGACTGGTCGGTGAGCTTCGAATTTCGCGGTGCCACGCTCAAACGCTTCAATCCGCTGCCTTACCTGTT
GCAGTTACTGCTTGAGGTGCATGGATTGCGGGCGCAGAGTGGCGCACTGTACAGCGTGCTGGCCGAGCTTTATTCCAACG
CGCTGGAGCATGGCGTGCTGGGCCTGGATTCGAGCCTCAAGCGTGATGCGTCGGGTTTCGCCCGATATTACGAGCAGCGC
AATGCCCGTCTCGAAGCCTTGCAGGACGGTTATGTGCGCGTGCATTTGCAGGTGCGGCCGGAAGGCGAGGGCGGTTGTCT
GGTGGTGCGGGTCGAAGACAGCGGCCAGGGATTCGATGTCTCGCGCGTGATGGAGCGGCCGCTGGACGGTGTCCGTCTGT
CGGGACGCGGAGTCAGCCTTATCCGGCAATTGGCCCGAAATGCCAGCTGGTCAGACGATGGTCGCAGTGCTCGCGTGGAG
TTTTTCTGGGAGGCTCTGGCATAA

Upstream 100 bases:

>100_bases
GCGACGATTCGGAAATCCGTGTGGTCAACAGCAGTTCAGATGTGAAGAAGATTCTCGCCATCTCCAACTTCGACAAACTG
TTCGACATCAGTTGACGGCC

Downstream 100 bases:

>100_bases
TCCGCGCATTCTTGATCAGGGAGTGAACAAGTGGCTGATACACATGTGGACCACGAGGCGCTGAGCGCGCTGCGGGAAGT
GATGGAAGACAGCTATCCGG

Product: response regulator receiver domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 567; Mature: 567

Protein sequence:

>567_residues
MQPQEPLTILIAEDSAADRLLLSTIVRRQGHEVLTATHGAEAVEAFIRQKPHLVLMDAMMPVMDGFEAARQIKKLAGENL
VPIIFLTSLTESEALARCLEAGGDDFLAKPYNQVILAAKIKAMDRLRRLQATVLQQRDLIAKHHDYLLNEQRVAKAVFDK
VAHSGCLSAPNIRYLQSPYALFNGDLLLAAYTPAGDMHVLLGDFTGHGLPAAVGAMPLAEVFYGMTAKGYGLAETLREMN
AKLKRILPVDMFCCATLLCLSFQRRSVEVWNGGMPDGYLHCLATGERKPLAARHLPLGVLSPQTFDDRTEVHSMAVGDRV
FLLSDGVIDTCDANDQLFGVERLHKVFAANRQPDRLFEDIEQALQDFRGEARDDVSMVEVSLLEAEQVNPPAPVYSDSGQ
SCPLDWSVSFEFRGATLKRFNPLPYLLQLLLEVHGLRAQSGALYSVLAELYSNALEHGVLGLDSSLKRDASGFARYYEQR
NARLEALQDGYVRVHLQVRPEGEGGCLVVRVEDSGQGFDVSRVMERPLDGVRLSGRGVSLIRQLARNASWSDDGRSARVE
FFWEALA

Sequences:

>Translated_567_residues
MQPQEPLTILIAEDSAADRLLLSTIVRRQGHEVLTATHGAEAVEAFIRQKPHLVLMDAMMPVMDGFEAARQIKKLAGENL
VPIIFLTSLTESEALARCLEAGGDDFLAKPYNQVILAAKIKAMDRLRRLQATVLQQRDLIAKHHDYLLNEQRVAKAVFDK
VAHSGCLSAPNIRYLQSPYALFNGDLLLAAYTPAGDMHVLLGDFTGHGLPAAVGAMPLAEVFYGMTAKGYGLAETLREMN
AKLKRILPVDMFCCATLLCLSFQRRSVEVWNGGMPDGYLHCLATGERKPLAARHLPLGVLSPQTFDDRTEVHSMAVGDRV
FLLSDGVIDTCDANDQLFGVERLHKVFAANRQPDRLFEDIEQALQDFRGEARDDVSMVEVSLLEAEQVNPPAPVYSDSGQ
SCPLDWSVSFEFRGATLKRFNPLPYLLQLLLEVHGLRAQSGALYSVLAELYSNALEHGVLGLDSSLKRDASGFARYYEQR
NARLEALQDGYVRVHLQVRPEGEGGCLVVRVEDSGQGFDVSRVMERPLDGVRLSGRGVSLIRQLARNASWSDDGRSARVE
FFWEALA
>Mature_567_residues
MQPQEPLTILIAEDSAADRLLLSTIVRRQGHEVLTATHGAEAVEAFIRQKPHLVLMDAMMPVMDGFEAARQIKKLAGENL
VPIIFLTSLTESEALARCLEAGGDDFLAKPYNQVILAAKIKAMDRLRRLQATVLQQRDLIAKHHDYLLNEQRVAKAVFDK
VAHSGCLSAPNIRYLQSPYALFNGDLLLAAYTPAGDMHVLLGDFTGHGLPAAVGAMPLAEVFYGMTAKGYGLAETLREMN
AKLKRILPVDMFCCATLLCLSFQRRSVEVWNGGMPDGYLHCLATGERKPLAARHLPLGVLSPQTFDDRTEVHSMAVGDRV
FLLSDGVIDTCDANDQLFGVERLHKVFAANRQPDRLFEDIEQALQDFRGEARDDVSMVEVSLLEAEQVNPPAPVYSDSGQ
SCPLDWSVSFEFRGATLKRFNPLPYLLQLLLEVHGLRAQSGALYSVLAELYSNALEHGVLGLDSSLKRDASGFARYYEQR
NARLEALQDGYVRVHLQVRPEGEGGCLVVRVEDSGQGFDVSRVMERPLDGVRLSGRGVSLIRQLARNASWSDDGRSARVE
FFWEALA

Specific function: Forms part of a two-component regulatory system AfsQ1/AfsQ2 involved in secondary metabolism [H]

COG id: COG2208

COG function: function code TK; Serine phosphatase RsbU, regulator of sigma subunit

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=128, Percent_Identity=29.6875, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1788550, Length=104, Percent_Identity=35.5769230769231, Blast_Score=66, Evalue=7e-12,
Organism=Escherichia coli, GI1789809, Length=125, Percent_Identity=32.8, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1788394, Length=132, Percent_Identity=33.3333333333333, Blast_Score=63, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 62703; Mature: 62703

Theoretical pI: Translated: 5.75; Mature: 5.75

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQPQEPLTILIAEDSAADRLLLSTIVRRQGHEVLTATHGAEAVEAFIRQKPHLVLMDAMM
CCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHCCCCEEEHHHHH
PVMDGFEAARQIKKLAGENLVPIIFLTSLTESEALARCLEAGGDDFLAKPYNQVILAAKI
HHHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
KAMDRLRRLQATVLQQRDLIAKHHDYLLNEQRVAKAVFDKVAHSGCLSAPNIRYLQSPYA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCHH
LFNGDLLLAAYTPAGDMHVLLGDFTGHGLPAAVGAMPLAEVFYGMTAKGYGLAETLREMN
HCCCCEEEEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
AKLKRILPVDMFCCATLLCLSFQRRSVEVWNGGMPDGYLHCLATGERKPLAARHLPLGVL
HHHHHHCCHHHHHHHHHHHHHHHCCCEEECCCCCCCCCEEEEECCCCCCCHHHCCCCCCC
SPQTFDDRTEVHSMAVGDRVFLLSDGVIDTCDANDQLFGVERLHKVFAANRQPDRLFEDI
CCCCCCCHHHHHHHHCCCEEEEEECCCEECCCCCCCCHHHHHHHHHHHCCCCHHHHHHHH
EQALQDFRGEARDDVSMVEVSLLEAEQVNPPAPVYSDSGQSCPLDWSVSFEFRGATLKRF
HHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEECCCHHHHC
NPLPYLLQLLLEVHGLRAQSGALYSVLAELYSNALEHGVLGLDSSLKRDASGFARYYEQR
CCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
NARLEALQDGYVRVHLQVRPEGEGGCLVVRVEDSGQGFDVSRVMERPLDGVRLSGRGVSL
CCHHHHHCCCEEEEEEEEEECCCCCEEEEEECCCCCCCCHHHHHHCCCCCEEECCCCHHH
IRQLARNASWSDDGRSARVEFFWEALA
HHHHHHCCCCCCCCCCHHHHHHHHHCC
>Mature Secondary Structure
MQPQEPLTILIAEDSAADRLLLSTIVRRQGHEVLTATHGAEAVEAFIRQKPHLVLMDAMM
CCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHCCCCEEEHHHHH
PVMDGFEAARQIKKLAGENLVPIIFLTSLTESEALARCLEAGGDDFLAKPYNQVILAAKI
HHHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
KAMDRLRRLQATVLQQRDLIAKHHDYLLNEQRVAKAVFDKVAHSGCLSAPNIRYLQSPYA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCHH
LFNGDLLLAAYTPAGDMHVLLGDFTGHGLPAAVGAMPLAEVFYGMTAKGYGLAETLREMN
HCCCCEEEEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
AKLKRILPVDMFCCATLLCLSFQRRSVEVWNGGMPDGYLHCLATGERKPLAARHLPLGVL
HHHHHHCCHHHHHHHHHHHHHHHCCCEEECCCCCCCCCEEEEECCCCCCCHHHCCCCCCC
SPQTFDDRTEVHSMAVGDRVFLLSDGVIDTCDANDQLFGVERLHKVFAANRQPDRLFEDI
CCCCCCCHHHHHHHHCCCEEEEEECCCEECCCCCCCCHHHHHHHHHHHCCCCHHHHHHHH
EQALQDFRGEARDDVSMVEVSLLEAEQVNPPAPVYSDSGQSCPLDWSVSFEFRGATLKRF
HHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEECCCHHHHC
NPLPYLLQLLLEVHGLRAQSGALYSVLAELYSNALEHGVLGLDSSLKRDASGFARYYEQR
CCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
NARLEALQDGYVRVHLQVRPEGEGGCLVVRVEDSGQGFDVSRVMERPLDGVRLSGRGVSL
CCHHHHHCCCEEEEEEEEEECCCCCEEEEEECCCCCCCCHHHHHHCCCCCEEECCCCHHH
IRQLARNASWSDDGRSARVEFFWEALA
HHHHHHCCCCCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1339426; 12000953 [H]