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The map label for this gene is fucA [C]

Identifier: 77457535

GI number: 77457535

Start: 1483012

End: 1483794

Strand: Reverse

Name: fucA [C]

Synonym: Pfl01_1308

Alternate gene names: 77457535

Gene position: 1483794-1483012 (Counterclockwise)

Preceding gene: 77457537

Following gene: 77457534

Centisome position: 23.05

GC content: 61.56

Gene sequence:

>783_bases
ATGGCGAAGACATTAGCACTACCCAAAGACGAACTGGTCAAGCAAGCGCTGACCCAGATGCAAAAAACCCTGGCGGATAA
TACGTGGACGGACCGGCAAAAGCTGGCGCTGACCTGCCGGATCCTGTTCGAGAACGGCCACGATTCGGGCCTCGCCGGGC
AGATCACCGCCCGTGGCCCGCAGCCAGGCACTTACTACACTCAGCAACTGGGCCTGGGTTTCGATGAAATCGCCGCGAGC
AATCTGCTGCTGGTCAACGAAGACCTGGAAGTGCTCGAAGGCCACGGCATGGCCAACCCGGCCAACCGTTTCCACAGCTG
GGTGTACCGCGCGCGGCCGGATGTGAACTGCATCATCCACACCCACCCGACACACATTGCCGCGCTGTCGATGCTGGAAG
TGCCGCTGCAGATTTCCCACATGGACCTGTGCCCGCTGTACGACGATTGCGCGTTCCTTGAAGGCTGGCCGGGTGTGCCG
GTGGGTAACGAAGAAGGCGAACTGATTGCCGGGGCGTTGGGTGACAAACGGGCGATCCTGCTTTCCCACCACGGGCAGCT
GTCGACCGGGACGACCATCGAGGAAGCCTGTGTCATCGCGCAACTGATCGAGCGCGCCGCCAAACTGCAATTGCTGGCGA
TGGCCGCCGGGACGATCAAACCGATCATTCCCGAACTGGGTCGCGAGGCCCATGACTGGGTGTCGAAACCCAAACGCCAC
GCCGCAGCCTTCAACTACTACGCCCGGCAGAACCTGCGTCAGCACGCCGATTGCCTGAACTGA

Upstream 100 bases:

>100_bases
ATTAATCCAGCAAAGGAAAGACGATTTGTCCTTGCCAATGACACAAAGTGTCATATAGCCTCATAAGTGTCATCCATGGC
AACCACCACAGGACACGGAT

Downstream 100 bases:

>100_bases
ACACCTCATCCTTGCGGAGCACTCTCATGTCCAACATTCACGGCATCATCGGCTACACCATCACCCCGTTCGGCGCCAAC
GGCGAAGGCGTTGACCTGCC

Product: class II aldolase/adducin domain-containing protein

Products: Dihydroxyacetone phosphate; L-Lactaldehyde [C]

Alternate protein names: NA

Number of amino acids: Translated: 260; Mature: 259

Protein sequence:

>260_residues
MAKTLALPKDELVKQALTQMQKTLADNTWTDRQKLALTCRILFENGHDSGLAGQITARGPQPGTYYTQQLGLGFDEIAAS
NLLLVNEDLEVLEGHGMANPANRFHSWVYRARPDVNCIIHTHPTHIAALSMLEVPLQISHMDLCPLYDDCAFLEGWPGVP
VGNEEGELIAGALGDKRAILLSHHGQLSTGTTIEEACVIAQLIERAAKLQLLAMAAGTIKPIIPELGREAHDWVSKPKRH
AAAFNYYARQNLRQHADCLN

Sequences:

>Translated_260_residues
MAKTLALPKDELVKQALTQMQKTLADNTWTDRQKLALTCRILFENGHDSGLAGQITARGPQPGTYYTQQLGLGFDEIAAS
NLLLVNEDLEVLEGHGMANPANRFHSWVYRARPDVNCIIHTHPTHIAALSMLEVPLQISHMDLCPLYDDCAFLEGWPGVP
VGNEEGELIAGALGDKRAILLSHHGQLSTGTTIEEACVIAQLIERAAKLQLLAMAAGTIKPIIPELGREAHDWVSKPKRH
AAAFNYYARQNLRQHADCLN
>Mature_259_residues
AKTLALPKDELVKQALTQMQKTLADNTWTDRQKLALTCRILFENGHDSGLAGQITARGPQPGTYYTQQLGLGFDEIAASN
LLLVNEDLEVLEGHGMANPANRFHSWVYRARPDVNCIIHTHPTHIAALSMLEVPLQISHMDLCPLYDDCAFLEGWPGVPV
GNEEGELIAGALGDKRAILLSHHGQLSTGTTIEEACVIAQLIERAAKLQLLAMAAGTIKPIIPELGREAHDWVSKPKRHA
AAFNYYARQNLRQHADCLN

Specific function: Fucose metabolism; third step. [C]

COG id: COG0235

COG function: function code G; Ribulose-5-phosphate 4-epimerase and related epimerases and aldolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aldolase class II family [H]

Homologues:

Organism=Escherichia coli, GI1789164, Length=203, Percent_Identity=28.0788177339901, Blast_Score=71, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI71989560, Length=208, Percent_Identity=31.7307692307692, Blast_Score=76, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI71989550, Length=208, Percent_Identity=31.7307692307692, Blast_Score=76, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI71989541, Length=208, Percent_Identity=31.7307692307692, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI71989555, Length=208, Percent_Identity=31.7307692307692, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI71989544, Length=208, Percent_Identity=31.7307692307692, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI115533216, Length=197, Percent_Identity=25.8883248730964, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI115533218, Length=195, Percent_Identity=26.6666666666667, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI281363755, Length=205, Percent_Identity=33.1707317073171, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI281363751, Length=205, Percent_Identity=33.1707317073171, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI24655690, Length=205, Percent_Identity=33.1707317073171, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI281363763, Length=210, Percent_Identity=32.8571428571429, Blast_Score=74, Evalue=8e-14,
Organism=Drosophila melanogaster, GI281363757, Length=210, Percent_Identity=32.8571428571429, Blast_Score=74, Evalue=8e-14,
Organism=Drosophila melanogaster, GI17136738, Length=210, Percent_Identity=32.8571428571429, Blast_Score=74, Evalue=8e-14,
Organism=Drosophila melanogaster, GI24655685, Length=205, Percent_Identity=33.1707317073171, Blast_Score=74, Evalue=8e-14,
Organism=Drosophila melanogaster, GI281363761, Length=205, Percent_Identity=33.1707317073171, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI281363759, Length=205, Percent_Identity=33.1707317073171, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI281363753, Length=205, Percent_Identity=33.1707317073171, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001303 [H]

Pfam domain/function: PF00596 Aldolase_II [H]

EC number: 4.1.2.17 [C]

Molecular weight: Translated: 28596; Mature: 28465

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKTLALPKDELVKQALTQMQKTLADNTWTDRQKLALTCRILFENGHDSGLAGQITARGP
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHEEEEEEEEECCCCCCCEEEEEECCC
QPGTYYTQQLGLGFDEIAASNLLLVNEDLEVLEGHGMANPANRFHSWVYRARPDVNCIIH
CCCCHHHHHHCCCHHHHHHCCEEEECCCHHHHHCCCCCCHHHHHHHHHHHCCCCCEEEEE
THPTHIAALSMLEVPLQISHMDLCPLYDDCAFLEGWPGVPVGNEEGELIAGALGDKRAIL
CCCHHHHHHHHHHCCCEECCCCCCCCCCCHHHHCCCCCCCCCCCCCCEEEECCCCCEEEE
LSHHGQLSTGTTIEEACVIAQLIERAAKLQLLAMAAGTIKPIIPELGREAHDWVSKPKRH
EECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHCHHHH
AAAFNYYARQNLRQHADCLN
HHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AKTLALPKDELVKQALTQMQKTLADNTWTDRQKLALTCRILFENGHDSGLAGQITARGP
CCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHEEEEEEEEECCCCCCCEEEEEECCC
QPGTYYTQQLGLGFDEIAASNLLLVNEDLEVLEGHGMANPANRFHSWVYRARPDVNCIIH
CCCCHHHHHHCCCHHHHHHCCEEEECCCHHHHHCCCCCCHHHHHHHHHHHCCCCCEEEEE
THPTHIAALSMLEVPLQISHMDLCPLYDDCAFLEGWPGVPVGNEEGELIAGALGDKRAIL
CCCHHHHHHHHHHCCCEECCCCCCCCCCCHHHHCCCCCCCCCCCCCCEEEECCCCCEEEE
LSHHGQLSTGTTIEEACVIAQLIERAAKLQLLAMAAGTIKPIIPELGREAHDWVSKPKRH
EECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHCHHHH
AAAFNYYARQNLRQHADCLN
HHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Ca2+; Co2+; Mg2+; Mn2+; Zn2+ [C]

Kcat value (1/min): 19.3 [C]

Specific activity: 21.0

Km value (mM): 0.7 {L-fuculose} [C]

Substrates: L-Fuculose 1-phosphate [C]

Specific reaction: L-Fuculose 1-phosphate <==> Dihydroxyacetone phosphate + L-Lactaldehyde [C]

General reaction: Elimination of an aldehyde C-C bond; Cleavage [C]

Inhibitor: EDTA; Phosphoglycolohydroxamate [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9823893 [H]