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The map label for this gene is argT [H]

Identifier: 77457437

GI number: 77457437

Start: 1381589

End: 1382371

Strand: Reverse

Name: argT [H]

Synonym: Pfl01_1210

Alternate gene names: 77457437

Gene position: 1382371-1381589 (Counterclockwise)

Preceding gene: 77457438

Following gene: 77457436

Centisome position: 21.47

GC content: 54.92

Gene sequence:

>783_bases
ATGAAAAAAGCATTGCTGACCCTTTCTGCACTGGCGTTGTGCATGGCCGCCGGCTCCGCGCTGGCCAAGGAATACAAAGA
ACTGCGGTTTGGCGTTGACCCTTCCTACGCACCGTTCGAGTCGAAAGCGGCAGACGGCAGCCTGGTTGGCTTCGACATCG
ATCTGGGCAACGCGATCTGCGCCGAGCTGAAGGTCAAGTGCAAATGGGTCGAAAGTGACTTCGACGGTATGATTCCGGGC
CTCAAGGCCAATAAATTCGACGGTGTGATCTCGTCGATGACCGTTACCGAAGCCCGTGAAAAAGTCATCGACTTCTCCAG
CGAGCTGTTCTCCGGCCCGACGGCCTACGTGTCGAAAAAAGGTTCGGGCATCACCGACGACGTCGCTTCCCTGAAAGGCA
AAACCGTCGGCTACGAGCAAGGCACCATCCAGGAAGCCTATGCCAAAGCCGTGCTGGACAAGGCCGGCGTGAAAACCCAG
GCCTATCAAAATCAGGACCAGGTGTATTCCGACCTGACCTCGGGCCGTCTCGATGCGGGCATTCAGGACATGTTGCAGGC
CGAACTGGGCTTTCTGAAGTCGCCGCAAGGCGCTGATTATGAAGTCAGCAAGCCGGTCGACAACCACTTGCTGCCAGCCA
AGACCGCTGTCGGTATTAAGAAAGGTAACACCGAGCTGAAAGCGCTTTTGGATAAAGGTATCAAAGCGTTACACGATGAC
GGCAAATACGCCGAGATTCAAAAGAAACACTTTGGCGATCTGAATCTGTACAGCGGCAAATAA

Upstream 100 bases:

>100_bases
GACTCTGGTTCGGAAATTGCTACTTTCTATAAGTCGTAAGCGCTCCAGTAACAATCATTCCGCGCCACAAGCGCTCATAT
TGGTTCTTAGGGATTGAATA

Downstream 100 bases:

>100_bases
TGCCCCGGCGCCCATCCTCGATGGGCGCCTTTTTCATTCAGTCAGGTTGCTGATTTATGTTCGAAACCCTCTTACAAAAT
CTGGGGCTCTCCGCCTTCAG

Product: extracellular solute-binding protein

Products: ADP; phosphate; L-ornithine [Cytoplasm]; ADP; L-arginine [Cytoplasm]; L-lysine [Cytoplasm] [C]

Alternate protein names: LAO-binding protein [H]

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MKKALLTLSALALCMAAGSALAKEYKELRFGVDPSYAPFESKAADGSLVGFDIDLGNAICAELKVKCKWVESDFDGMIPG
LKANKFDGVISSMTVTEAREKVIDFSSELFSGPTAYVSKKGSGITDDVASLKGKTVGYEQGTIQEAYAKAVLDKAGVKTQ
AYQNQDQVYSDLTSGRLDAGIQDMLQAELGFLKSPQGADYEVSKPVDNHLLPAKTAVGIKKGNTELKALLDKGIKALHDD
GKYAEIQKKHFGDLNLYSGK

Sequences:

>Translated_260_residues
MKKALLTLSALALCMAAGSALAKEYKELRFGVDPSYAPFESKAADGSLVGFDIDLGNAICAELKVKCKWVESDFDGMIPG
LKANKFDGVISSMTVTEAREKVIDFSSELFSGPTAYVSKKGSGITDDVASLKGKTVGYEQGTIQEAYAKAVLDKAGVKTQ
AYQNQDQVYSDLTSGRLDAGIQDMLQAELGFLKSPQGADYEVSKPVDNHLLPAKTAVGIKKGNTELKALLDKGIKALHDD
GKYAEIQKKHFGDLNLYSGK
>Mature_260_residues
MKKALLTLSALALCMAAGSALAKEYKELRFGVDPSYAPFESKAADGSLVGFDIDLGNAICAELKVKCKWVESDFDGMIPG
LKANKFDGVISSMTVTEAREKVIDFSSELFSGPTAYVSKKGSGITDDVASLKGKTVGYEQGTIQEAYAKAVLDKAGVKTQ
AYQNQDQVYSDLTSGRLDAGIQDMLQAELGFLKSPQGADYEVSKPVDNHLLPAKTAVGIKKGNTELKALLDKGIKALHDD
GKYAEIQKKHFGDLNLYSGK

Specific function: This periplasmic binding protein is involved in an arginine transport system. ArgT and histidine-binding protein J (hisJ) interact with a common membrane-bound receptor, hisP [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788649, Length=259, Percent_Identity=46.7181467181467, Blast_Score=226, Evalue=1e-60,
Organism=Escherichia coli, GI1788648, Length=236, Percent_Identity=42.7966101694915, Blast_Score=203, Evalue=1e-53,
Organism=Escherichia coli, GI1787085, Length=252, Percent_Identity=32.5396825396825, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI1787088, Length=252, Percent_Identity=30.952380952381, Blast_Score=120, Evalue=7e-29,
Organism=Escherichia coli, GI1787031, Length=253, Percent_Identity=33.201581027668, Blast_Score=114, Evalue=4e-27,
Organism=Escherichia coli, GI1788228, Length=261, Percent_Identity=28.735632183908, Blast_Score=97, Evalue=1e-21,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 27862; Mature: 27862

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKALLTLSALALCMAAGSALAKEYKELRFGVDPSYAPFESKAADGSLVGFDIDLGNAIC
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCCHHHH
AELKVKCKWVESDFDGMIPGLKANKFDGVISSMTVTEAREKVIDFSSELFSGPTAYVSKK
HHHEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCC
GSGITDDVASLKGKTVGYEQGTIQEAYAKAVLDKAGVKTQAYQNQDQVYSDLTSGRLDAG
CCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHCCCCCHH
IQDMLQAELGFLKSPQGADYEVSKPVDNHLLPAKTAVGIKKGNTELKALLDKGIKALHDD
HHHHHHHHHHHCCCCCCCCEECCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHCC
GKYAEIQKKHFGDLNLYSGK
CCHHHHHHHHCCCCEECCCC
>Mature Secondary Structure
MKKALLTLSALALCMAAGSALAKEYKELRFGVDPSYAPFESKAADGSLVGFDIDLGNAIC
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCCHHHH
AELKVKCKWVESDFDGMIPGLKANKFDGVISSMTVTEAREKVIDFSSELFSGPTAYVSKK
HHHEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCC
GSGITDDVASLKGKTVGYEQGTIQEAYAKAVLDKAGVKTQAYQNQDQVYSDLTSGRLDAG
CCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHCCCCCHH
IQDMLQAELGFLKSPQGADYEVSKPVDNHLLPAKTAVGIKKGNTELKALLDKGIKALHDD
HHHHHHHHHHHCCCCCCCCEECCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHCC
GKYAEIQKKHFGDLNLYSGK
CCHHHHHHHHCCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-ornithine [Periplasm]; H2O; ATP; L-arginine [Periplasm]; L-lysine [Periplasm] [C]

Specific reaction: ATP + L-ornithine [Periplasm] + H2O = ADP + phosphate + L-ornithine [Cytoplasm] ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] ATP + L-lysine [Periplasm] + H2O = ADP + phosphate + L-lysine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 3040734; 9298646 [H]