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The map label for this gene is holC [H]

Identifier: 77457215

GI number: 77457215

Start: 1148810

End: 1149238

Strand: Reverse

Name: holC [H]

Synonym: Pfl01_0988

Alternate gene names: 77457215

Gene position: 1149238-1148810 (Counterclockwise)

Preceding gene: 77457216

Following gene: 77457214

Centisome position: 17.85

GC content: 59.44

Gene sequence:

>429_bases
ATGACCAAAGTCGACTTTTATATCCTGCCCAGCGCCGATCCTTCGGCTCGCCTGGACTTTGCCTGCAAGCTCACCGATAA
AGCCTGGCGCATGGGCCATCGCATCTACCTGCATTGCAGCGATGCCGCCCAGCGTGATGATCTCGATGCGCGCCTGTGGG
CGTTCAAGGGTGAAACCTTCGTGCCTCACGGCCCGGCTGAAAGCGAGCCGGAAGGTTTGATCGTATTGGGAGTGGGCGAG
GACTGTGGCCAGCACACAGACTTGCTGGTCAACCTCGACCTGAAAGTCCCGGTTTTCGCCAGTAAATTCGCCCGCGTTGC
CGAGGTGGTGGTGGAAGATCCGGCGATCCGCACTGCGGCGCGGGAGAGTTTCCGTTTCTACCGCGAACAGGGCTATCCTC
TGCAAGACCACCGTTTACAGCGACTCTGA

Upstream 100 bases:

>100_bases
GCTGTTGACCCAATACTTGCTGGACCGCGCCAAAGCCTGAAACCGATGACTGCGAGCGGCGCCTCTCTTGAGGGGCGCCG
CTCGCAGCTCAGGAACCGCA

Downstream 100 bases:

>100_bases
GTACGCCGATGGACACTCCAAAACCGCTGCAAAAACCCGCGCACCTGCTGGATGACCTTGAGTCGATCCGCCAGTTGCTC
GGCGATGACAACCTGCAACC

Product: DNA polymerase III subunit chi

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 142; Mature: 141

Protein sequence:

>142_residues
MTKVDFYILPSADPSARLDFACKLTDKAWRMGHRIYLHCSDAAQRDDLDARLWAFKGETFVPHGPAESEPEGLIVLGVGE
DCGQHTDLLVNLDLKVPVFASKFARVAEVVVEDPAIRTAARESFRFYREQGYPLQDHRLQRL

Sequences:

>Translated_142_residues
MTKVDFYILPSADPSARLDFACKLTDKAWRMGHRIYLHCSDAAQRDDLDARLWAFKGETFVPHGPAESEPEGLIVLGVGE
DCGQHTDLLVNLDLKVPVFASKFARVAEVVVEDPAIRTAARESFRFYREQGYPLQDHRLQRL
>Mature_141_residues
TKVDFYILPSADPSARLDFACKLTDKAWRMGHRIYLHCSDAAQRDDLDARLWAFKGETFVPHGPAESEPEGLIVLGVGED
CGQHTDLLVNLDLKVPVFASKFARVAEVVVEDPAIRTAARESFRFYREQGYPLQDHRLQRL

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity [H]

COG id: COG2927

COG function: function code L; DNA polymerase III, chi subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790709, Length=142, Percent_Identity=29.5774647887324, Blast_Score=69, Evalue=9e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007459 [H]

Pfam domain/function: PF04364 DNA_pol3_chi [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 16102; Mature: 15971

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKVDFYILPSADPSARLDFACKLTDKAWRMGHRIYLHCSDAAQRDDLDARLWAFKGETF
CCCEEEEEECCCCCCCCEEEEEEECHHHHHCCCEEEEEECCCCCCCCCCEEEEEEECCEE
VPHGPAESEPEGLIVLGVGEDCGQHTDLLVNLDLKVPVFASKFARVAEVVVEDPAIRTAA
CCCCCCCCCCCCEEEEECCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCHHHHH
RESFRFYREQGYPLQDHRLQRL
HHHHHHHHHCCCCCCHHHHCCC
>Mature Secondary Structure 
TKVDFYILPSADPSARLDFACKLTDKAWRMGHRIYLHCSDAAQRDDLDARLWAFKGETF
CCEEEEEECCCCCCCCEEEEEEECHHHHHCCCEEEEEECCCCCCCCCCEEEEEEECCEE
VPHGPAESEPEGLIVLGVGEDCGQHTDLLVNLDLKVPVFASKFARVAEVVVEDPAIRTAA
CCCCCCCCCCCCEEEEECCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCHHHHH
RESFRFYREQGYPLQDHRLQRL
HHHHHHHHHCCCCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9864319; 10984043 [H]