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The map label for this gene is rlmF

Identifier: 77457212

GI number: 77457212

Start: 1144204

End: 1145226

Strand: Reverse

Name: rlmF

Synonym: Pfl01_0985

Alternate gene names: 77457212

Gene position: 1145226-1144204 (Counterclockwise)

Preceding gene: 77457213

Following gene: 77457210

Centisome position: 17.79

GC content: 62.37

Gene sequence:

>1023_bases
ATGAACGCCCCACGTACACCGAAACCTGCGCGCAAGAAGCCTGACTCCGCCACCCCGGCCAAGCCCGTGGAGCCGCGTAA
AGAAGCCAGCCTGCACCCGCGCAATCGCCATCAGGGTCGTTACGACTTCCCGGCGCTGATCAAGACCACTCCGGAACTGG
CGAAGTTCGTGATCACCAACCCGTACGGCAAGGAAAGCATCGATTTCGCCAGTCCCGATGCGGTGCGTGTGTTCAACCGG
GCGCTGCTCAAATCGTTCTACGGCATCCAGCATTGGGACATCCCGGCCGATTATCTGTGCCCGCCGGTGCCGGGCCGTGC
CGACTACATCCACTTTTTGGCTGACCTGCTGGCCAGCAACAACGACGGCGTGGTTCCGCGCGGCGCCATCGTCAATGTGC
TGGATATCGGCATGGGCGCCAACTGCGTGTACCCGCTGATCGGTAACAGCGAATATCGCTGGCACTTCCTCGGCTCGGAG
ATCGATCCGACCGCCGTGGCCGCTGCCAGGGCCATCGTTCAGTCCAACGACCTGAACAAGGTCATCCAGCTGCGCCAGCA
GGAGAACCGCAAGCACATCCTGATCGGCCTGCTGGAGCCCGGCGAACGCTTTGACCTGACCATGTGCAACCCGCCATTCC
ATGCCTCGATGGAAGAGGCCACCAAGGGCAGCGAGCGCAAATGGCGAGCCCTGGGCAAGGCCGACCCGAAGCGCAAATTG
CCGGTACTGAACTTCGGCGGCCAGTCGGCCGAGCTGTGGTGTGAAGGTGGCGAGGCGCGTTTCGTGACGCAACTGATCGC
CGAAAGTGCGAACTTTGCACACAAGGTGCTGTGGTTCAGCACCCTGGTCTCGAAAGCCTCGAACCTGCCTGCCATCGAAA
CCGCGCTGAAGAAGGCCGGTGCGCTGGAAAGCCAGGTGGTGGAAATGTCCCAGGGGCAGAAGCAGAGCCGCTTCGTCGCC
TGGACCTTCCAGACCAAGTCCGAGCAGCAGATCTGGCGGCGCGAACGCTGGGTGCGCAAGTAA

Upstream 100 bases:

>100_bases
CGGCCTCCCTTTTTTGTGCCCGGCACTTTTCCGGCCAACAACGCAGCGCCCTGTGGGACAATAGCCGCCACTTTCAGCCC
TACCCGAATCGACCACGCCC

Downstream 100 bases:

>100_bases
TACGTTTCAACCTGGGCGGCGCTTGACCGCAAGCAGCCGCCGCTACTGCTGAAACACAAACAGCAGGCACAAAAAAACCG
TGCCCGGATCGCTCCGGTGC

Product: putative SAM-dependent methyltransferase

Products: NA

Alternate protein names: 23S rRNA mA1618 methyltransferase; rRNA adenine N-6-methyltransferase

Number of amino acids: Translated: 340; Mature: 340

Protein sequence:

>340_residues
MNAPRTPKPARKKPDSATPAKPVEPRKEASLHPRNRHQGRYDFPALIKTTPELAKFVITNPYGKESIDFASPDAVRVFNR
ALLKSFYGIQHWDIPADYLCPPVPGRADYIHFLADLLASNNDGVVPRGAIVNVLDIGMGANCVYPLIGNSEYRWHFLGSE
IDPTAVAAARAIVQSNDLNKVIQLRQQENRKHILIGLLEPGERFDLTMCNPPFHASMEEATKGSERKWRALGKADPKRKL
PVLNFGGQSAELWCEGGEARFVTQLIAESANFAHKVLWFSTLVSKASNLPAIETALKKAGALESQVVEMSQGQKQSRFVA
WTFQTKSEQQIWRRERWVRK

Sequences:

>Translated_340_residues
MNAPRTPKPARKKPDSATPAKPVEPRKEASLHPRNRHQGRYDFPALIKTTPELAKFVITNPYGKESIDFASPDAVRVFNR
ALLKSFYGIQHWDIPADYLCPPVPGRADYIHFLADLLASNNDGVVPRGAIVNVLDIGMGANCVYPLIGNSEYRWHFLGSE
IDPTAVAAARAIVQSNDLNKVIQLRQQENRKHILIGLLEPGERFDLTMCNPPFHASMEEATKGSERKWRALGKADPKRKL
PVLNFGGQSAELWCEGGEARFVTQLIAESANFAHKVLWFSTLVSKASNLPAIETALKKAGALESQVVEMSQGQKQSRFVA
WTFQTKSEQQIWRRERWVRK
>Mature_340_residues
MNAPRTPKPARKKPDSATPAKPVEPRKEASLHPRNRHQGRYDFPALIKTTPELAKFVITNPYGKESIDFASPDAVRVFNR
ALLKSFYGIQHWDIPADYLCPPVPGRADYIHFLADLLASNNDGVVPRGAIVNVLDIGMGANCVYPLIGNSEYRWHFLGSE
IDPTAVAAARAIVQSNDLNKVIQLRQQENRKHILIGLLEPGERFDLTMCNPPFHASMEEATKGSERKWRALGKADPKRKL
PVLNFGGQSAELWCEGGEARFVTQLIAESANFAHKVLWFSTLVSKASNLPAIETALKKAGALESQVVEMSQGQKQSRFVA
WTFQTKSEQQIWRRERWVRK

Specific function: Specifically methylates the adenine in position 1618 of 23S rRNA

COG id: COG3129

COG function: function code R; Predicted SAM-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. METT10D/rlmF family

Homologues:

Organism=Homo sapiens, GI122114654, Length=296, Percent_Identity=31.0810810810811, Blast_Score=163, Evalue=2e-40,
Organism=Escherichia coli, GI87081786, Length=311, Percent_Identity=51.4469453376206, Blast_Score=326, Evalue=1e-90,
Organism=Caenorhabditis elegans, GI32564931, Length=305, Percent_Identity=28.5245901639344, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI32564933, Length=305, Percent_Identity=26.5573770491803, Blast_Score=105, Evalue=4e-23,
Organism=Drosophila melanogaster, GI281363415, Length=311, Percent_Identity=28.2958199356913, Blast_Score=119, Evalue=2e-27,
Organism=Drosophila melanogaster, GI19922302, Length=311, Percent_Identity=28.2958199356913, Blast_Score=119, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMF_PSEPF (Q3KHM8)

Other databases:

- EMBL:   CP000094
- RefSeq:   YP_346717.1
- ProteinModelPortal:   Q3KHM8
- SMR:   Q3KHM8
- STRING:   Q3KHM8
- GeneID:   3715968
- GenomeReviews:   CP000094_GR
- KEGG:   pfo:Pfl01_0985
- eggNOG:   COG3129
- HOGENOM:   HBG747880
- OMA:   AELWCEG
- ProtClustDB:   PRK11727
- BioCyc:   PFLU205922:PFL_0985-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01848
- InterPro:   IPR016909
- InterPro:   IPR010286
- PANTHER:   PTHR13393
- PIRSF:   PIRSF029038

Pfam domain/function: PF05971 Methyltransf_10

EC number: =2.1.1.181

Molecular weight: Translated: 38148; Mature: 38148

Theoretical pI: Translated: 10.07; Mature: 10.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAPRTPKPARKKPDSATPAKPVEPRKEASLHPRNRHQGRYDFPALIKTTPELAKFVITN
CCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCHHHHCCHHHHHHHHCC
PYGKESIDFASPDAVRVFNRALLKSFYGIQHWDIPADYLCPPVPGRADYIHFLADLLASN
CCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHCC
NDGVVPRGAIVNVLDIGMGANCVYPLIGNSEYRWHFLGSEIDPTAVAAARAIVQSNDLNK
CCCCCCCCHHHHHHHCCCCCCEEEEEECCCCEEEEECCCCCCHHHHHHHHHHHHCCCHHH
VIQLRQQENRKHILIGLLEPGERFDLTMCNPPFHASMEEATKGSERKWRALGKADPKRKL
HHHHHHHCCCCEEEEEEECCCCCEEEEECCCCCCCCHHHHHCCCHHHHHHHCCCCCCCCC
PVLNFGGQSAELWCEGGEARFVTQLIAESANFAHKVLWFSTLVSKASNLPAIETALKKAG
CEEECCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHC
ALESQVVEMSQGQKQSRFVAWTFQTKSEQQIWRRERWVRK
CHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MNAPRTPKPARKKPDSATPAKPVEPRKEASLHPRNRHQGRYDFPALIKTTPELAKFVITN
CCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCHHHHCCHHHHHHHHCC
PYGKESIDFASPDAVRVFNRALLKSFYGIQHWDIPADYLCPPVPGRADYIHFLADLLASN
CCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHCC
NDGVVPRGAIVNVLDIGMGANCVYPLIGNSEYRWHFLGSEIDPTAVAAARAIVQSNDLNK
CCCCCCCCHHHHHHHCCCCCCEEEEEECCCCEEEEECCCCCCHHHHHHHHHHHHCCCHHH
VIQLRQQENRKHILIGLLEPGERFDLTMCNPPFHASMEEATKGSERKWRALGKADPKRKL
HHHHHHHCCCCEEEEEEECCCCCEEEEECCCCCCCCHHHHHCCCHHHHHHHCCCCCCCCC
PVLNFGGQSAELWCEGGEARFVTQLIAESANFAHKVLWFSTLVSKASNLPAIETALKKAG
CEEECCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHC
ALESQVVEMSQGQKQSRFVAWTFQTKSEQQIWRRERWVRK
CHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA