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The map label for this gene is cysD [H]

Identifier: 77457104

GI number: 77457104

Start: 1023859

End: 1024776

Strand: Direct

Name: cysD [H]

Synonym: Pfl01_0877

Alternate gene names: 77457104

Gene position: 1023859-1024776 (Clockwise)

Preceding gene: 77457103

Following gene: 77457105

Centisome position: 15.9

GC content: 59.15

Gene sequence:

>918_bases
ATGGTCGACAAACTGACGCATCTGAAACAGCTGGAGGCGGAAAGCATCCACATCATCCGCGAGGTGGCCGCCGAGTTCGA
CAACCCGGTGATGCTGTACTCCATCGGTAAAGACTCCGCCGTGATGCTGCATCTGGCACGCAAGGCGTTCTTCCCGGGCA
AACTGCCGTTTCCGGTGATGCACGTCGACACCCGCTGGAAATTCCAGGAGATGTACAAGTTCCGCGACAAGATGGTCGAA
GAGCTTGGCCTGGACCTCATCACTCACATCAACCCCGACGGCGTGGCGCAGAACATCAACCCGTTCACCCACGGCAGCGC
CAAGCACACCGACATCATGAAGACCGAGGGCCTGAAGCAGGCACTCGACAAGCATGGTTTCGACGCAGCCTTCGGCGGTG
CCCGTCGCGATGAAGAGAAGTCCCGCGCCAAGGAGCGCGTCTACTCGTTCCGTGACAGCAAACACCGCTGGGACCCGAAG
AACCAGCGTCCCGAGCTGTGGAACGTCTACAACGGCAAGGTCAACAAGGGCGAATCCATTCGTGTGTTCCCGCTGTCGAA
CTGGACCGAGCTGGACATCTGGCAGTACATCTACCTCGAAGGCATCCCGATCGTACCGCTGTACTTCGCCGCCGAGCGCG
AAGTGATCGAGAAGAACGGCACGCTGATCATGATCGACGACGAGCGCATCCTCGAGCACCTGTCCGACGAGGACAAGGCG
CGCATCGTCAAAAAGAAAGTGCGTTTCCGTACCCTTGGCTGCTACCCGTTGACGGGCGCGGTGGAGTCCGAGGCCGAAAG
CCTGACGGACATCATTCAGGAAATGCTCCTGACGCGAACTTCCGAGCGCCAGGGCCGGGTCATCGACCACGATGGCGCAG
GCTCGATGGAAGACAAGAAACGTCAGGGTTATTTCTAA

Upstream 100 bases:

>100_bases
CGATCTAGTTGGCGTCCTGATTAGAAGAGGTCGCTGTGCTAGGATTCCTCGCTCGAACACGGCCCGCTGGCCGTTCATAA
GAAAGCTTTCGTGAGTAGCC

Downstream 100 bases:

>100_bases
GGGGTTGTCATGTCGCACGCATCTGATTTGATCAGCGAGGACATCCTCGCCTACCTGGGCCAGCACGAACGTAAAGAGCT
GCTGCGCTTTTTGACCTGCG

Product: sulfate adenylyltransferase subunit 2

Products: NA

Alternate protein names: ATP-sulfurylase small subunit; Sulfate adenylate transferase; SAT [H]

Number of amino acids: Translated: 305; Mature: 305

Protein sequence:

>305_residues
MVDKLTHLKQLEAESIHIIREVAAEFDNPVMLYSIGKDSAVMLHLARKAFFPGKLPFPVMHVDTRWKFQEMYKFRDKMVE
ELGLDLITHINPDGVAQNINPFTHGSAKHTDIMKTEGLKQALDKHGFDAAFGGARRDEEKSRAKERVYSFRDSKHRWDPK
NQRPELWNVYNGKVNKGESIRVFPLSNWTELDIWQYIYLEGIPIVPLYFAAEREVIEKNGTLIMIDDERILEHLSDEDKA
RIVKKKVRFRTLGCYPLTGAVESEAESLTDIIQEMLLTRTSERQGRVIDHDGAGSMEDKKRQGYF

Sequences:

>Translated_305_residues
MVDKLTHLKQLEAESIHIIREVAAEFDNPVMLYSIGKDSAVMLHLARKAFFPGKLPFPVMHVDTRWKFQEMYKFRDKMVE
ELGLDLITHINPDGVAQNINPFTHGSAKHTDIMKTEGLKQALDKHGFDAAFGGARRDEEKSRAKERVYSFRDSKHRWDPK
NQRPELWNVYNGKVNKGESIRVFPLSNWTELDIWQYIYLEGIPIVPLYFAAEREVIEKNGTLIMIDDERILEHLSDEDKA
RIVKKKVRFRTLGCYPLTGAVESEAESLTDIIQEMLLTRTSERQGRVIDHDGAGSMEDKKRQGYF
>Mature_305_residues
MVDKLTHLKQLEAESIHIIREVAAEFDNPVMLYSIGKDSAVMLHLARKAFFPGKLPFPVMHVDTRWKFQEMYKFRDKMVE
ELGLDLITHINPDGVAQNINPFTHGSAKHTDIMKTEGLKQALDKHGFDAAFGGARRDEEKSRAKERVYSFRDSKHRWDPK
NQRPELWNVYNGKVNKGESIRVFPLSNWTELDIWQYIYLEGIPIVPLYFAAEREVIEKNGTLIMIDDERILEHLSDEDKA
RIVKKKVRFRTLGCYPLTGAVESEAESLTDIIQEMLLTRTSERQGRVIDHDGAGSMEDKKRQGYF

Specific function: First step in the sulfate activation pathway. This reaction occurs early in the reductive branch of the cysteine biosynthetic pathway. [C]

COG id: COG0175

COG function: function code EH; 3'-phosphoadenosine 5'-phosphosulfate sulfotransferase (PAPS reductase)/FAD synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PAPS reductase family. CysD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789109, Length=303, Percent_Identity=75.5775577557756, Blast_Score=481, Evalue=1e-137,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002500
- InterPro:   IPR014729
- InterPro:   IPR011784 [H]

Pfam domain/function: PF01507 PAPS_reduct [H]

EC number: =2.7.7.4 [H]

Molecular weight: Translated: 35322; Mature: 35322

Theoretical pI: Translated: 6.93; Mature: 6.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVDKLTHLKQLEAESIHIIREVAAEFDNPVMLYSIGKDSAVMLHLARKAFFPGKLPFPVM
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHCCCCCCCCCEE
HVDTRWKFQEMYKFRDKMVEELGLDLITHINPDGVAQNINPFTHGSAKHTDIMKTEGLKQ
EECCCHHHHHHHHHHHHHHHHHCCHHHHCCCCCCHHCCCCCCCCCCCCCCHHHHHHHHHH
ALDKHGFDAAFGGARRDEEKSRAKERVYSFRDSKHRWDPKNQRPELWNVYNGKVNKGESI
HHHHCCCCHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCCCCEE
RVFPLSNWTELDIWQYIYLEGIPIVPLYFAAEREVIEKNGTLIMIDDERILEHLSDEDKA
EEEECCCCCCHHHHHHHHHCCCCCEEEEHHHHHHHHHCCCCEEEEECHHHHHHCCCHHHH
RIVKKKVRFRTLGCYPLTGAVESEAESLTDIIQEMLLTRTSERQGRVIDHDGAGSMEDKK
HHHHHHHHHHEECCEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCHHH
RQGYF
HCCCC
>Mature Secondary Structure
MVDKLTHLKQLEAESIHIIREVAAEFDNPVMLYSIGKDSAVMLHLARKAFFPGKLPFPVM
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHCCCCCCCCCEE
HVDTRWKFQEMYKFRDKMVEELGLDLITHINPDGVAQNINPFTHGSAKHTDIMKTEGLKQ
EECCCHHHHHHHHHHHHHHHHHCCHHHHCCCCCCHHCCCCCCCCCCCCCCHHHHHHHHHH
ALDKHGFDAAFGGARRDEEKSRAKERVYSFRDSKHRWDPKNQRPELWNVYNGKVNKGESI
HHHHCCCCHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCCCCEE
RVFPLSNWTELDIWQYIYLEGIPIVPLYFAAEREVIEKNGTLIMIDDERILEHLSDEDKA
EEEECCCCCCHHHHHHHHHCCCCCEEEEHHHHHHHHHCCCCEEEEECHHHHHHCCCHHHH
RIVKKKVRFRTLGCYPLTGAVESEAESLTDIIQEMLLTRTSERQGRVIDHDGAGSMEDKK
HHHHHHHHHHEECCEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCHHH
RQGYF
HCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA