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The map label for this gene is mlaE [H]

Identifier: 77457091

GI number: 77457091

Start: 1011577

End: 1012374

Strand: Direct

Name: mlaE [H]

Synonym: Pfl01_0864

Alternate gene names: 77457091

Gene position: 1011577-1012374 (Clockwise)

Preceding gene: 77457090

Following gene: 77457092

Centisome position: 15.71

GC content: 58.9

Gene sequence:

>798_bases
ATGCGCAGAATTACATTAATGGAGCGTGTGCGTCGCTTTGGCCTCGCCGGAATCGACAGCGTCGCAGTGTTCGGGCGTTC
GACCCTGTTCCTGTTTCATGCCTTGCTGGGTCGTGGCGGCATCGGCGGCGGTTTCGGCCTGCTGGTCAAACAGCTGCATT
CCGTCGGCGTGATGTCGCTGGTGATCATCGTGGTGTCGGGGATTTTCATCGGCATGGTGCTGGCCCTGCAGGGCTTCAAC
ATCCTGTCGAGCTACGGCTCGGAGCAGGCTGTCGGGCAGATGGTGGCGCTGACCCTGTTACGTGAGCTGGGGCCGGTGGT
GACCGCGCTGTTGTTTGCCGGTCGTGCCGGTTCGGCGCTGACGGCGGAAATCGGCAACATGAAATCCACCGAACAGCTGT
CCAGCCTGGAGATGATAGGGGTCGACCCGCTCAAGTACATCATCGCCCCACGCCTTTGGGCCGGCTTCATTTCCCTGCCG
GTACTGGCGATGATCTTCAGTGTGGTCGGCATCTGGGGCGGTTCGTGGGTTGCCGTCGACTGGCTGGGCGTCTACGAAGG
TTCCTACTGGTCGAACATGCAGAACAGCGTGAGCTTCGGTGACGATGTGCTCAACGGCATCATCAAAAGTGCAGTATTCG
CTTTTGTCGTCACCTGGATCGCCGTATTTCAAGGCTATGACTGCGAACCCACTTCCGAGGGGATCAGTCGTGCCACCACC
AAGACCGTGGTTTACGCCTCGCTGGCAGTACTCGGCCTGGACTTTATTCTGACCGCTTTGATGTTTGGAGATTTCTGA

Upstream 100 bases:

>100_bases
CGCGTATCCGTCAATTCATGACCGGTGAACCCGACGGCCCGGTGCCGTATCACTTTCCAGCGCCGGATTACCGCGCCGAT
CTTCTGGGGAAGCGCCGCTG

Downstream 100 bases:

>100_bases
TGCAAAACCGCACCCTGGAAATCGGTGTCGGCCTGTTCCTGCTGGCAGGGATCCTGGCTTTGTTGCTGCTGGCCCTGCGG
GTCAGTGGCCTGTCTCCGAC

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 265; Mature: 265

Protein sequence:

>265_residues
MRRITLMERVRRFGLAGIDSVAVFGRSTLFLFHALLGRGGIGGGFGLLVKQLHSVGVMSLVIIVVSGIFIGMVLALQGFN
ILSSYGSEQAVGQMVALTLLRELGPVVTALLFAGRAGSALTAEIGNMKSTEQLSSLEMIGVDPLKYIIAPRLWAGFISLP
VLAMIFSVVGIWGGSWVAVDWLGVYEGSYWSNMQNSVSFGDDVLNGIIKSAVFAFVVTWIAVFQGYDCEPTSEGISRATT
KTVVYASLAVLGLDFILTALMFGDF

Sequences:

>Translated_265_residues
MRRITLMERVRRFGLAGIDSVAVFGRSTLFLFHALLGRGGIGGGFGLLVKQLHSVGVMSLVIIVVSGIFIGMVLALQGFN
ILSSYGSEQAVGQMVALTLLRELGPVVTALLFAGRAGSALTAEIGNMKSTEQLSSLEMIGVDPLKYIIAPRLWAGFISLP
VLAMIFSVVGIWGGSWVAVDWLGVYEGSYWSNMQNSVSFGDDVLNGIIKSAVFAFVVTWIAVFQGYDCEPTSEGISRATT
KTVVYASLAVLGLDFILTALMFGDF
>Mature_265_residues
MRRITLMERVRRFGLAGIDSVAVFGRSTLFLFHALLGRGGIGGGFGLLVKQLHSVGVMSLVIIVVSGIFIGMVLALQGFN
ILSSYGSEQAVGQMVALTLLRELGPVVTALLFAGRAGSALTAEIGNMKSTEQLSSLEMIGVDPLKYIIAPRLWAGFISLP
VLAMIFSVVGIWGGSWVAVDWLGVYEGSYWSNMQNSVSFGDDVLNGIIKSAVFAFVVTWIAVFQGYDCEPTSEGISRATT
KTVVYASLAVLGLDFILTALMFGDF

Specific function: Part of the ABC transporter complex mlaFEDB that actively prevents phospholipid accumulation at the cell surface. Probably maintains lipid asymmetry in the outer membrane by retrograde trafficking of phospholipids from the outer membrane to the inner memb

COG id: COG0767

COG function: function code Q; ABC-type transport system involved in resistance to organic solvents, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mlaE permease family [H]

Homologues:

Organism=Escherichia coli, GI1789585, Length=259, Percent_Identity=61.3899613899614, Blast_Score=316, Evalue=1e-87,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003453 [H]

Pfam domain/function: PF02405 DUF140 [H]

EC number: NA

Molecular weight: Translated: 28335; Mature: 28335

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]