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The map label for this gene is lutR [H]

Identifier: 77457050

GI number: 77457050

Start: 971291

End: 972115

Strand: Direct

Name: lutR [H]

Synonym: Pfl01_0823

Alternate gene names: 77457050

Gene position: 971291-972115 (Clockwise)

Preceding gene: 77457049

Following gene: 77457054

Centisome position: 15.09

GC content: 63.88

Gene sequence:

>825_bases
GTGGAAACGGAAAGTGATCGTACAACCCGCTTGAGCTATGATGCCCACAAGCCGAATTTTCGAGACAAGATGCAAACCAT
GGAAAACACGATCGACACCCCGCGTCTCCCTCGCAAGCGCCGCAGCCTGGCTCAGGAACTGGTGACGGTGCTGACCGAGC
AGATCCGCGACGGTCTGCTCAAGCGCGGCGACAAGTTGCCGACCGAGTCGGCGATCATGGAGGCCCATGGCGTCAGCCGC
ACCGTGGTGCGCGAGGCGATTTCCCGTTTGCAGGCGGCGGGGCAGGTGGAAACCCGTCATGGCATCGGCACCTTCGTGCT
CGACACCCCGAGCCCGAGCGGTTTCCGGATCGACCCGGCCACCGTGGTCACCCTGCGTGACGTGCTGGCCATTCTCGAAC
TGCGTATCAGCCTCGAAGTCGAATCCGCCGGCCTCGCCGCACAGCGCCGCAGCGCCGAGCAACTTGCTGCGATGCGCGCG
GCCCTTGATGCGCTGAATGACAGCGTGGCCCACGCCAGCGATGCAGTGGCGTCGGACTTCCAGTTCCATCTGCAAATCGC
GCTGTCCACCGGCAACCGCTATTTCACCGACATCATGACCCACCTGGGCACCAGCATCATTCCGCGCACTCGCCTGAATT
CGGCGCGCCTGGCCCATGACGATCAGCAGCACTACATGAATCGCCTGAGCCGCGAACACGAAGAAATCTACGACGCCATC
GCCCGTCAGGATTCCGATGCCGCCCGTGCGGCCATGCGCCTGCACCTGACCAACAGCCGCGAACGGCTGCGTCAGGCGCA
TGAAGAGGCGCAGGCGCAGGGGTGA

Upstream 100 bases:

>100_bases
TGCGGATCGACAGTGGTCACCTGGTTGAGCAGAACGTGACCGCCTGATATATCCACACAAACCCTGTGGGGGCTGGCTTG
CCGCCTCCCACAGGGTTTGT

Downstream 100 bases:

>100_bases
GAAATCAGGCTTCGATGTAGAGTTTCAGTGGTTCCGAACACGGCTCCAGACTGGCCTCATCAGCAAACTTGTAACGCAGT
TCGACGGTGTTGTTCAGGTA

Product: GntR family transcriptional regulator

Products: NA

Alternate protein names: L-lactate utilization operon repressor [H]

Number of amino acids: Translated: 274; Mature: 274

Protein sequence:

>274_residues
METESDRTTRLSYDAHKPNFRDKMQTMENTIDTPRLPRKRRSLAQELVTVLTEQIRDGLLKRGDKLPTESAIMEAHGVSR
TVVREAISRLQAAGQVETRHGIGTFVLDTPSPSGFRIDPATVVTLRDVLAILELRISLEVESAGLAAQRRSAEQLAAMRA
ALDALNDSVAHASDAVASDFQFHLQIALSTGNRYFTDIMTHLGTSIIPRTRLNSARLAHDDQQHYMNRLSREHEEIYDAI
ARQDSDAARAAMRLHLTNSRERLRQAHEEAQAQG

Sequences:

>Translated_274_residues
METESDRTTRLSYDAHKPNFRDKMQTMENTIDTPRLPRKRRSLAQELVTVLTEQIRDGLLKRGDKLPTESAIMEAHGVSR
TVVREAISRLQAAGQVETRHGIGTFVLDTPSPSGFRIDPATVVTLRDVLAILELRISLEVESAGLAAQRRSAEQLAAMRA
ALDALNDSVAHASDAVASDFQFHLQIALSTGNRYFTDIMTHLGTSIIPRTRLNSARLAHDDQQHYMNRLSREHEEIYDAI
ARQDSDAARAAMRLHLTNSRERLRQAHEEAQAQG
>Mature_274_residues
METESDRTTRLSYDAHKPNFRDKMQTMENTIDTPRLPRKRRSLAQELVTVLTEQIRDGLLKRGDKLPTESAIMEAHGVSR
TVVREAISRLQAAGQVETRHGIGTFVLDTPSPSGFRIDPATVVTLRDVLAILELRISLEVESAGLAAQRRSAEQLAAMRA
ALDALNDSVAHASDAVASDFQFHLQIALSTGNRYFTDIMTHLGTSIIPRTRLNSARLAHDDQQHYMNRLSREHEEIYDAI
ARQDSDAARAAMRLHLTNSRERLRQAHEEAQAQG

Specific function: Negatively regulates the transcription of the lutABC operon, which is required for L-lactate utilization. LutR activity is regulated by lactate, since presence of L-lactate, that probably binds to lutR, leads to derepression of the operon. Also appears to

COG id: COG2186

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790780, Length=219, Percent_Identity=31.9634703196347, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI87082208, Length=222, Percent_Identity=30.6306306306306, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI48994955, Length=227, Percent_Identity=27.7533039647577, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1789353, Length=237, Percent_Identity=25.7383966244726, Blast_Score=62, Evalue=6e-11,
Organism=Escherichia coli, GI1789621, Length=231, Percent_Identity=29.004329004329, Blast_Score=61, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011711
- InterPro:   IPR000524
- InterPro:   IPR011991 [H]

Pfam domain/function: PF07729 FCD; PF00392 GntR [H]

EC number: NA

Molecular weight: Translated: 30683; Mature: 30683

Theoretical pI: Translated: 7.15; Mature: 7.15

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METESDRTTRLSYDAHKPNFRDKMQTMENTIDTPRLPRKRRSLAQELVTVLTEQIRDGLL
CCCCCCCCEEEECCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
KRGDKLPTESAIMEAHGVSRTVVREAISRLQAAGQVETRHGIGTFVLDTPSPSGFRIDPA
HCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHCCCCEEEEEECCCCCCEEECHH
TVVTLRDVLAILELRISLEVESAGLAAQRRSAEQLAAMRAALDALNDSVAHASDAVASDF
HHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
QFHLQIALSTGNRYFTDIMTHLGTSIIPRTRLNSARLAHDDQQHYMNRLSREHEEIYDAI
EEEEEEEEECCCHHHHHHHHHHCCHHCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHH
ARQDSDAARAAMRLHLTNSRERLRQAHEEAQAQG
HCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
METESDRTTRLSYDAHKPNFRDKMQTMENTIDTPRLPRKRRSLAQELVTVLTEQIRDGLL
CCCCCCCCEEEECCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
KRGDKLPTESAIMEAHGVSRTVVREAISRLQAAGQVETRHGIGTFVLDTPSPSGFRIDPA
HCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHCCCCEEEEEECCCCCCEEECHH
TVVTLRDVLAILELRISLEVESAGLAAQRRSAEQLAAMRAALDALNDSVAHASDAVASDF
HHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
QFHLQIALSTGNRYFTDIMTHLGTSIIPRTRLNSARLAHDDQQHYMNRLSREHEEIYDAI
EEEEEEEEECCCHHHHHHHHHHCCHHCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHH
ARQDSDAARAAMRLHLTNSRERLRQAHEEAQAQG
HCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]