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The map label for this gene is grpE

Identifier: 77456989

GI number: 77456989

Start: 894250

End: 894819

Strand: Direct

Name: grpE

Synonym: Pfl01_0762

Alternate gene names: 77456989

Gene position: 894250-894819 (Clockwise)

Preceding gene: 77456987

Following gene: 77456990

Centisome position: 13.89

GC content: 60.35

Gene sequence:

>570_bases
ATGGCTGACGAACAGACAGTAGATACGCAAAATCCAGAAGCCAATCAGGCGCCCGAGGCCTCGGGTGATGACCTGGCGAC
CCGTGTACAAGTGCTCGAAGAGCAATTGGCCGCCGCGCAGGATCAATCGCTGCGCGTAGCTGCCGATCTGCAGAACGTCC
GCCGCCGTGCCGAGCAGGATGTCGAAAAGGCGCACAAGTTTGCGCTGGAAAAATTCGCCGGCGATCTGCTGCCGATCGTC
GACAGCCTGGAGCGTGGTCTCGAGCTGTCGAGCCCGGATGACGAAAGCATCCGTCCGATGCGCGAAGGCATCGAGCTGAC
CCTGAAAATGTTTCACGACACCCTCACGCGCTATCAGCTTGTCGCTGTCAGCCCGCAGGAAGGCGAACCGTTCAATGCCG
TGGAGCATCAGGCGATGGCCATGCAGGAAAGTGCTGACCTGGAGCCGAACAGCATTCTGAAGGTGTTCCAGAAGGGCTAC
CAGCTCAACGGTCGCCTGCTGCGTCCGGCGATGGTCGTGGTCAGCAAGGCGCCGGCGCCAGTTTCGCCTTCGATTGACGA
GAAGGCTTGA

Upstream 100 bases:

>100_bases
GGGTTAATCGTTGTCAGCCCTTGAAGCCTGATTTTGCGGCCCCATATATCGGGGCAGAAGCGCGAATCGAACTCGCGGAC
GATATTGAAAGGAGAATTCT

Downstream 100 bases:

>100_bases
AATTAGCCGCAAGGCCCCCATTTACAAGTCAAGCGTTTAAGTATTACCGCAGTCAGCCACCACTGCTGCGGCAAAAAAAT
CCAAAGTTTCGGGAGAGTGA

Product: heat shock protein GrpE

Products: NA

Alternate protein names: HSP-70 cofactor

Number of amino acids: Translated: 189; Mature: 188

Protein sequence:

>189_residues
MADEQTVDTQNPEANQAPEASGDDLATRVQVLEEQLAAAQDQSLRVAADLQNVRRRAEQDVEKAHKFALEKFAGDLLPIV
DSLERGLELSSPDDESIRPMREGIELTLKMFHDTLTRYQLVAVSPQEGEPFNAVEHQAMAMQESADLEPNSILKVFQKGY
QLNGRLLRPAMVVVSKAPAPVSPSIDEKA

Sequences:

>Translated_189_residues
MADEQTVDTQNPEANQAPEASGDDLATRVQVLEEQLAAAQDQSLRVAADLQNVRRRAEQDVEKAHKFALEKFAGDLLPIV
DSLERGLELSSPDDESIRPMREGIELTLKMFHDTLTRYQLVAVSPQEGEPFNAVEHQAMAMQESADLEPNSILKVFQKGY
QLNGRLLRPAMVVVSKAPAPVSPSIDEKA
>Mature_188_residues
ADEQTVDTQNPEANQAPEASGDDLATRVQVLEEQLAAAQDQSLRVAADLQNVRRRAEQDVEKAHKFALEKFAGDLLPIVD
SLERGLELSSPDDESIRPMREGIELTLKMFHDTLTRYQLVAVSPQEGEPFNAVEHQAMAMQESADLEPNSILKVFQKGYQ
LNGRLLRPAMVVVSKAPAPVSPSIDEKA

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family

Homologues:

Organism=Homo sapiens, GI24308295, Length=150, Percent_Identity=32, Blast_Score=82, Evalue=2e-16,
Organism=Homo sapiens, GI40255109, Length=138, Percent_Identity=28.9855072463768, Blast_Score=64, Evalue=1e-10,
Organism=Escherichia coli, GI1788967, Length=169, Percent_Identity=49.112426035503, Blast_Score=145, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI17552458, Length=172, Percent_Identity=30.8139534883721, Blast_Score=67, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6324806, Length=160, Percent_Identity=33.75, Blast_Score=89, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24653432, Length=173, Percent_Identity=28.9017341040462, Blast_Score=77, Evalue=5e-15,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GRPE_PSEPF (Q3KIA1)

Other databases:

- EMBL:   CP000094
- RefSeq:   YP_346494.1
- ProteinModelPortal:   Q3KIA1
- SMR:   Q3KIA1
- STRING:   Q3KIA1
- GeneID:   3715220
- GenomeReviews:   CP000094_GR
- KEGG:   pfo:Pfl01_0762
- eggNOG:   COG0576
- HOGENOM:   HBG732630
- OMA:   NVRRRME
- ProtClustDB:   PRK14151
- BioCyc:   PFLU205922:PFL_0762-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01151
- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012
- Gene3D:   G3DSA:3.90.20.20
- Gene3D:   G3DSA:2.30.22.10
- PANTHER:   PTHR21237
- PRINTS:   PR00773

Pfam domain/function: PF01025 GrpE; SSF51064 GrpE_head

EC number: NA

Molecular weight: Translated: 20861; Mature: 20729

Theoretical pI: Translated: 4.33; Mature: 4.33

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADEQTVDTQNPEANQAPEASGDDLATRVQVLEEQLAAAQDQSLRVAADLQNVRRRAEQD
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
VEKAHKFALEKFAGDLLPIVDSLERGLELSSPDDESIRPMREGIELTLKMFHDTLTRYQL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEE
VAVSPQEGEPFNAVEHQAMAMQESADLEPNSILKVFQKGYQLNGRLLRPAMVVVSKAPAP
EEECCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEHHHHHHHHHCCCCC
VSPSIDEKA
CCCCCCCCC
>Mature Secondary Structure 
ADEQTVDTQNPEANQAPEASGDDLATRVQVLEEQLAAAQDQSLRVAADLQNVRRRAEQD
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
VEKAHKFALEKFAGDLLPIVDSLERGLELSSPDDESIRPMREGIELTLKMFHDTLTRYQL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEE
VAVSPQEGEPFNAVEHQAMAMQESADLEPNSILKVFQKGYQLNGRLLRPAMVVVSKAPAP
EEECCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEHHHHHHHHHCCCCC
VSPSIDEKA
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA