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The map label for this gene is yfiN [H]

Identifier: 77456920

GI number: 77456920

Start: 818335

End: 819603

Strand: Direct

Name: yfiN [H]

Synonym: Pfl01_0692

Alternate gene names: 77456920

Gene position: 818335-819603 (Clockwise)

Preceding gene: 77456919

Following gene: 77456921

Centisome position: 12.71

GC content: 63.28

Gene sequence:

>1269_bases
ATGAGACGCGACAAATCCACAGGGCGCCCGACGCTGGGTTCGGTCATCGGCCGTGGCAACCTGATTGTGGCGCTGGTCGC
CGTGGCAATGGCCAGTGTGTCGCTGACGCTGCTTGGGGTACTGGCGCTGCGGGTCTATGCCGATCACAACCTGCACTTGA
TCGCCCGCTCGATCAGTTACACGGTTGAAGCTGCCGTGGTGTTCAACGACAAGGCCGCTGCGACCGAAGCGCTGGCGCTG
ATCGCCTCGACCGAAGAAGTGGCCGATGCCCAGGTGCTGGACACTCAGGGCCAGTTGCTCGCGCGCTGGCAGCGTCCGGA
GAACGGTCTGTTTTCCGAGCTGGAGATGCAAGTCGCGCGGGTCATACTGGAAAAACCCATCAACCTGCCGATCCTCCATC
AGGATCGCGAGATCGGGCGTATCTTGCTGATCGGCCACGGTGGCAGCCTGATGCGCTTTCTGTTGAGCGGTCTGGCGGGG
ATCATTCTCTGCACGGCGATCAGCGCTTGGGTCGCGCTGTACCTGGCGCAGCGTCAGTTGCGCAGGATCATCGGTCCGTT
GCACAGCCTCGCAGCGGTGGCGCATGCGGCCCGCAGCGAGCGGGCACTGGATCGACGGGTGCCACGGGCGAAGATTGCCG
AACTGGACAACCTCGGCAATGACTTCAATGCCTTGCTCGATGAACTCGAAGACTGGCAAACCCACCTGCAAAGCGAGAAC
GAAACCCTCGCCCACCAGGCCAGCCACGACAGTCTCACCGGCTTGCCGAACCGGGCCTTTTTCGAAGGCCGCCTGATCCG
TGCGCTACGCAGCGCCCACAAACTCAACGAGCGCGTGGCGGTGCTGTTTCTCGACAGCGACCGCTTCAAGGACATCAACG
ACAACTTCGGTCATGCCGCCGGTGACGCGGTGCTGGTGGCGGTGGCGAATCGAGTGCGGGCGCAGTTGCGTGAAGAGGAT
CTGGTGGCGCGTCTGGGCGGTGACGAGTTCGCCGTTCTGCTCACGCCCTTGCACAAGACCGAAGACGCCGAACGCATTGC
CGACAAGATCCTCGCCAGCATGGACACCCCGATTGCGCTGCCGGGCGACAGCAGTGTCGTGACGTCGCTCAGTATCGGCA
TCGCGGTTTACCCCGATCATGGCGTCACGCCCGGCACCTTGCTTGATGCGGCCGATGCCGCGATGTATCAAGCCAAGCGT
TTGTCGCGTGGCGCCCAATCCACGGCCGGGTCGGAGCACCCGGTCGATCCCGTTCAAACCAGGAGCTGA

Upstream 100 bases:

>100_bases
ACCAGGTGTCGTTCGAGGTCAATCTCGACTCCGTCGCCCGTAGCGGTGTGCGCATTCATCCCAGCGTGCTGCAGTTGTCG
CGCCGCAAACCGGCGGTGCC

Downstream 100 bases:

>100_bases
TGCCCGTGTTCTCACTTTCCTTTCGACTGTTTTCCACCCTGTTGCTGACGGCCATGCTGGCCCTGACCGGTTGCCAGACC
GCCCCGCAAAAAGGCCTGAC

Product: diguanylate cyclase

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 422; Mature: 422

Protein sequence:

>422_residues
MRRDKSTGRPTLGSVIGRGNLIVALVAVAMASVSLTLLGVLALRVYADHNLHLIARSISYTVEAAVVFNDKAAATEALAL
IASTEEVADAQVLDTQGQLLARWQRPENGLFSELEMQVARVILEKPINLPILHQDREIGRILLIGHGGSLMRFLLSGLAG
IILCTAISAWVALYLAQRQLRRIIGPLHSLAAVAHAARSERALDRRVPRAKIAELDNLGNDFNALLDELEDWQTHLQSEN
ETLAHQASHDSLTGLPNRAFFEGRLIRALRSAHKLNERVAVLFLDSDRFKDINDNFGHAAGDAVLVAVANRVRAQLREED
LVARLGGDEFAVLLTPLHKTEDAERIADKILASMDTPIALPGDSSVVTSLSIGIAVYPDHGVTPGTLLDAADAAMYQAKR
LSRGAQSTAGSEHPVDPVQTRS

Sequences:

>Translated_422_residues
MRRDKSTGRPTLGSVIGRGNLIVALVAVAMASVSLTLLGVLALRVYADHNLHLIARSISYTVEAAVVFNDKAAATEALAL
IASTEEVADAQVLDTQGQLLARWQRPENGLFSELEMQVARVILEKPINLPILHQDREIGRILLIGHGGSLMRFLLSGLAG
IILCTAISAWVALYLAQRQLRRIIGPLHSLAAVAHAARSERALDRRVPRAKIAELDNLGNDFNALLDELEDWQTHLQSEN
ETLAHQASHDSLTGLPNRAFFEGRLIRALRSAHKLNERVAVLFLDSDRFKDINDNFGHAAGDAVLVAVANRVRAQLREED
LVARLGGDEFAVLLTPLHKTEDAERIADKILASMDTPIALPGDSSVVTSLSIGIAVYPDHGVTPGTLLDAADAAMYQAKR
LSRGAQSTAGSEHPVDPVQTRS
>Mature_422_residues
MRRDKSTGRPTLGSVIGRGNLIVALVAVAMASVSLTLLGVLALRVYADHNLHLIARSISYTVEAAVVFNDKAAATEALAL
IASTEEVADAQVLDTQGQLLARWQRPENGLFSELEMQVARVILEKPINLPILHQDREIGRILLIGHGGSLMRFLLSGLAG
IILCTAISAWVALYLAQRQLRRIIGPLHSLAAVAHAARSERALDRRVPRAKIAELDNLGNDFNALLDELEDWQTHLQSEN
ETLAHQASHDSLTGLPNRAFFEGRLIRALRSAHKLNERVAVLFLDSDRFKDINDNFGHAAGDAVLVAVANRVRAQLREED
LVARLGGDEFAVLLTPLHKTEDAERIADKILASMDTPIALPGDSSVVTSLSIGIAVYPDHGVTPGTLLDAADAAMYQAKR
LSRGAQSTAGSEHPVDPVQTRS

Specific function: Cyclic-di-GMP is a second messenger which controls cell surface-associated traits in bacteria [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HAMP domain [H]

Homologues:

Organism=Escherichia coli, GI1788956, Length=402, Percent_Identity=34.5771144278607, Blast_Score=220, Evalue=2e-58,
Organism=Escherichia coli, GI1788381, Length=163, Percent_Identity=41.1042944785276, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI1787541, Length=158, Percent_Identity=39.2405063291139, Blast_Score=102, Evalue=5e-23,
Organism=Escherichia coli, GI1787262, Length=179, Percent_Identity=33.5195530726257, Blast_Score=96, Evalue=6e-21,
Organism=Escherichia coli, GI145693134, Length=212, Percent_Identity=33.0188679245283, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI87081977, Length=190, Percent_Identity=36.3157894736842, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1786584, Length=178, Percent_Identity=31.4606741573034, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI87082007, Length=167, Percent_Identity=37.125748502994, Blast_Score=82, Evalue=9e-17,
Organism=Escherichia coli, GI87081881, Length=164, Percent_Identity=35.3658536585366, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1787816, Length=177, Percent_Identity=29.3785310734463, Blast_Score=76, Evalue=5e-15,
Organism=Escherichia coli, GI1787802, Length=178, Percent_Identity=32.0224719101124, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1788085, Length=171, Percent_Identity=28.0701754385965, Blast_Score=68, Evalue=9e-13,
Organism=Escherichia coli, GI87081974, Length=104, Percent_Identity=33.6538461538462, Blast_Score=65, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR003660 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 45680; Mature: 45680

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS50885 HAMP ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRDKSTGRPTLGSVIGRGNLIVALVAVAMASVSLTLLGVLALRVYADHNLHLIARSISY
CCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHH
TVEAAVVFNDKAAATEALALIASTEEVADAQVLDTQGQLLARWQRPENGLFSELEMQVAR
EEEEEEEECCCHHHHHHHHHHHCHHHHHHHHHHCCCCHHHHHHCCCCCCHHHHHHHHHHH
VILEKPINLPILHQDREIGRILLIGHGGSLMRFLLSGLAGIILCTAISAWVALYLAQRQL
HHHHCCCCCCEEECCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RRIIGPLHSLAAVAHAARSERALDRRVPRAKIAELDNLGNDFNALLDELEDWQTHLQSEN
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCC
ETLAHQASHDSLTGLPNRAFFEGRLIRALRSAHKLNERVAVLFLDSDRFKDINDNFGHAA
HHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCC
GDAVLVAVANRVRAQLREEDLVARLGGDEFAVLLTPLHKTEDAERIADKILASMDTPIAL
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCEEC
PGDSSVVTSLSIGIAVYPDHGVTPGTLLDAADAAMYQAKRLSRGAQSTAGSEHPVDPVQT
CCCCHHHHEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
RS
CC
>Mature Secondary Structure
MRRDKSTGRPTLGSVIGRGNLIVALVAVAMASVSLTLLGVLALRVYADHNLHLIARSISY
CCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHH
TVEAAVVFNDKAAATEALALIASTEEVADAQVLDTQGQLLARWQRPENGLFSELEMQVAR
EEEEEEEECCCHHHHHHHHHHHCHHHHHHHHHHCCCCHHHHHHCCCCCCHHHHHHHHHHH
VILEKPINLPILHQDREIGRILLIGHGGSLMRFLLSGLAGIILCTAISAWVALYLAQRQL
HHHHCCCCCCEEECCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RRIIGPLHSLAAVAHAARSERALDRRVPRAKIAELDNLGNDFNALLDELEDWQTHLQSEN
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCC
ETLAHQASHDSLTGLPNRAFFEGRLIRALRSAHKLNERVAVLFLDSDRFKDINDNFGHAA
HHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCC
GDAVLVAVANRVRAQLREEDLVARLGGDEFAVLLTPLHKTEDAERIADKILASMDTPIAL
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCEEC
PGDSSVVTSLSIGIAVYPDHGVTPGTLLDAADAAMYQAKRLSRGAQSTAGSEHPVDPVQT
CCCCHHHHEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
RS
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 6357787; 7567469 [H]