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The map label for this gene is braD [H]

Identifier: 77456817

GI number: 77456817

Start: 679786

End: 681288

Strand: Reverse

Name: braD [H]

Synonym: Pfl01_0589

Alternate gene names: 77456817

Gene position: 681288-679786 (Counterclockwise)

Preceding gene: 77456818

Following gene: 77456816

Centisome position: 10.58

GC content: 65.07

Gene sequence:

>1503_bases
ATGCCCACTGCCCTTTACCGCTTGATCCTGGCCGTCGCACTTTTGCTGCCGATGGCCGTCCATGCCGGGGATGCGGAAGA
CTTCGTCGCCGCCAACCCGATGCAACAGGCCAAACTGCTGGAAGCCTGGGCCGCGCAGCCCGATCCGGCGCGTGGCGAAC
TGATCAACGCCCTGCAACAAGGCGAACTGACCGTCGACGGCCAACCCAAGACCCTGCGCCTGAACAACCGCCTGCGGGGT
CTGATCGACACCGCGCTGGCCAGCCACCAGTTGCTCGCCGCCGACGCCAAAACCCGTCTGACTGCCGCGCAGCAATTGCA
GAAAAGCGCCAAACCGGCGCAGCTGAAATTCCTCGACCGGCAACTGGCTGGCGAAATCGATGAAAGCGTCCACGCCGCCC
TGAGCCTGGCACTGGCCAACCTGCAACTGGTCGACACTGACCCGGCGGTGCGCCTCGCGGCCGTGCGTCTGCTCGGTGAA
ACCGGCGACCCGCTGGCTCGCACCCGCCTCGAAGGCTTGCTCGAACCCGGCATCGAAACCGATGCCAACGTGCGCACCGC
CGCCGAAACCAGCCTCGCCCAGGTCAAGCGCAAACTGCTGGTCGGCGAACTGCTCGGCCAGGCGTTCAGCGGTATGTCCC
TCGGTTCGATTCTGCTGCTCGCGGCACTCGGCCTGGCGATCACCTTCGGCCTGCTCGGCGTGATCAACATGGCCCACGGC
GAGATGCTGATGCTCGGCGCCTACTCGACCTACGTCGTGCAGTTGATGTTCCAGCGCTACGCCCCGCAGGCCATCGAGTT
CTATCCACTGATTGCGCTGCCGGTGGCGTTCTTCGTCACGGCCGCCATCGGCATGGCCCTGGAGCGCACGGTGATCCGTC
ACCTCTACGGCCGCCCGCTGGAAACCCTGCTCGCCACCTGGGGCATCAGCCTGATGCTGATTCAGCTGGTGCGTCTGGTG
TTCGGTGCGCAAAACGTGGAAGTGGCAAATCCGGCATGGCTGTCCGGCGGGATTCAGGTGTTGCCGAATCTGGTGCTGCC
GTACAACCGCATCGTGATCATCGCCTTCGCCTTGTTTGTGGTGGTGCTGACCTGGCTGCTGCTGAACAAGACCCGCCTCG
GTCTGAACGTGCGTGCCGTCACCCAGAACCGCAACATGGCCGCCTGCTGCGGCGTGCCCACCGGGCGCGTCGACATGCTC
GCCTTCGGCCTCGGCTCGGGCATTGCCGGGCTCGGCGGCGTGGCGCTGAGCCAGATCGGCAACGTCGGCCCGGACCTCGG
CCAGAGCTACATCATCGACTCGTTCCTGGTGGTGGTGCTCGGCGGCGTCGGCCAACTGGCCGGCAGCGTGTTCGCCGCGT
TCGGCCTGGGCATCGCCAACAAGATTCTCGAACCGCAGATCGGTGCGGTGCTCGGCAAGATCCTGATCCTCGCGCTGATC
ATTCTGTTCATCCAGAAACGTCCGCAAGGCCTCTTCGCACTGAAAGGACGGGTGATCGACTGA

Upstream 100 bases:

>100_bases
GCCTCAATGGACGCTCTGCGTCCGCTTTTGGGACGCGGAGCGTCCCGGGCTGCATTCCCACGCGGAGCGTGGGAACGATC
ACTACGCAAGGCCATTTCAT

Downstream 100 bases:

>100_bases
TGAACCAGCCTCTACTAGTCACGGCCACGCAAAAGGCCGGCCCCAAAGTCACCCTCGCCGTCGGCGTGGTCGTTCTCGCG
TTGCTGATCGCCCTGCCGCT

Product: inner-membrane translocator

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 500; Mature: 499

Protein sequence:

>500_residues
MPTALYRLILAVALLLPMAVHAGDAEDFVAANPMQQAKLLEAWAAQPDPARGELINALQQGELTVDGQPKTLRLNNRLRG
LIDTALASHQLLAADAKTRLTAAQQLQKSAKPAQLKFLDRQLAGEIDESVHAALSLALANLQLVDTDPAVRLAAVRLLGE
TGDPLARTRLEGLLEPGIETDANVRTAAETSLAQVKRKLLVGELLGQAFSGMSLGSILLLAALGLAITFGLLGVINMAHG
EMLMLGAYSTYVVQLMFQRYAPQAIEFYPLIALPVAFFVTAAIGMALERTVIRHLYGRPLETLLATWGISLMLIQLVRLV
FGAQNVEVANPAWLSGGIQVLPNLVLPYNRIVIIAFALFVVVLTWLLLNKTRLGLNVRAVTQNRNMAACCGVPTGRVDML
AFGLGSGIAGLGGVALSQIGNVGPDLGQSYIIDSFLVVVLGGVGQLAGSVFAAFGLGIANKILEPQIGAVLGKILILALI
ILFIQKRPQGLFALKGRVID

Sequences:

>Translated_500_residues
MPTALYRLILAVALLLPMAVHAGDAEDFVAANPMQQAKLLEAWAAQPDPARGELINALQQGELTVDGQPKTLRLNNRLRG
LIDTALASHQLLAADAKTRLTAAQQLQKSAKPAQLKFLDRQLAGEIDESVHAALSLALANLQLVDTDPAVRLAAVRLLGE
TGDPLARTRLEGLLEPGIETDANVRTAAETSLAQVKRKLLVGELLGQAFSGMSLGSILLLAALGLAITFGLLGVINMAHG
EMLMLGAYSTYVVQLMFQRYAPQAIEFYPLIALPVAFFVTAAIGMALERTVIRHLYGRPLETLLATWGISLMLIQLVRLV
FGAQNVEVANPAWLSGGIQVLPNLVLPYNRIVIIAFALFVVVLTWLLLNKTRLGLNVRAVTQNRNMAACCGVPTGRVDML
AFGLGSGIAGLGGVALSQIGNVGPDLGQSYIIDSFLVVVLGGVGQLAGSVFAAFGLGIANKILEPQIGAVLGKILILALI
ILFIQKRPQGLFALKGRVID
>Mature_499_residues
PTALYRLILAVALLLPMAVHAGDAEDFVAANPMQQAKLLEAWAAQPDPARGELINALQQGELTVDGQPKTLRLNNRLRGL
IDTALASHQLLAADAKTRLTAAQQLQKSAKPAQLKFLDRQLAGEIDESVHAALSLALANLQLVDTDPAVRLAAVRLLGET
GDPLARTRLEGLLEPGIETDANVRTAAETSLAQVKRKLLVGELLGQAFSGMSLGSILLLAALGLAITFGLLGVINMAHGE
MLMLGAYSTYVVQLMFQRYAPQAIEFYPLIALPVAFFVTAAIGMALERTVIRHLYGRPLETLLATWGISLMLIQLVRLVF
GAQNVEVANPAWLSGGIQVLPNLVLPYNRIVIIAFALFVVVLTWLLLNKTRLGLNVRAVTQNRNMAACCGVPTGRVDMLA
FGLGSGIAGLGGVALSQIGNVGPDLGQSYIIDSFLVVVLGGVGQLAGSVFAAFGLGIANKILEPQIGAVLGKILILALII
LFIQKRPQGLFALKGRVID

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0559

COG function: function code E; Branched-chain amino acid ABC-type transport system, permease components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789866, Length=293, Percent_Identity=27.6450511945392, Blast_Score=90, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851 [H]

Pfam domain/function: PF02653 BPD_transp_2 [H]

EC number: NA

Molecular weight: Translated: 53131; Mature: 53000

Theoretical pI: Translated: 9.28; Mature: 9.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTALYRLILAVALLLPMAVHAGDAEDFVAANPMQQAKLLEAWAAQPDPARGELINALQQ
CCHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHHHHHC
GELTVDGQPKTLRLNNRLRGLIDTALASHQLLAADAKTRLTAAQQLQKSAKPAQLKFLDR
CCEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
QLAGEIDESVHAALSLALANLQLVDTDPAVRLAAVRLLGETGDPLARTRLEGLLEPGIET
HHHHHHHHHHHHHHHHHHHHCEEECCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCC
DANVRTAAETSLAQVKRKLLVGELLGQAFSGMSLGSILLLAALGLAITFGLLGVINMAHG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
EMLMLGAYSTYVVQLMFQRYAPQAIEFYPLIALPVAFFVTAAIGMALERTVIRHLYGRPL
CEEEEHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
ETLLATWGISLMLIQLVRLVFGAQNVEVANPAWLSGGIQVLPNLVLPYNRIVIIAFALFV
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCHHHHHHHHCCHHHHHHHHHHHHH
VVLTWLLLNKTRLGLNVRAVTQNRNMAACCGVPTGRVDMLAFGLGSGIAGLGGVALSQIG
HHHHHHHHHHHHCCCEEEEEECCCCEEEECCCCCCCHHHHHHHHCCCCHHHHHHHHHHHC
NVGPDLGQSYIIDSFLVVVLGGVGQLAGSVFAAFGLGIANKILEPQIGAVLGKILILALI
CCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
ILFIQKRPQGLFALKGRVID
HHHHHCCCCCEEEECCCCCC
>Mature Secondary Structure 
PTALYRLILAVALLLPMAVHAGDAEDFVAANPMQQAKLLEAWAAQPDPARGELINALQQ
CHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHHHHHC
GELTVDGQPKTLRLNNRLRGLIDTALASHQLLAADAKTRLTAAQQLQKSAKPAQLKFLDR
CCEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
QLAGEIDESVHAALSLALANLQLVDTDPAVRLAAVRLLGETGDPLARTRLEGLLEPGIET
HHHHHHHHHHHHHHHHHHHHCEEECCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCC
DANVRTAAETSLAQVKRKLLVGELLGQAFSGMSLGSILLLAALGLAITFGLLGVINMAHG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
EMLMLGAYSTYVVQLMFQRYAPQAIEFYPLIALPVAFFVTAAIGMALERTVIRHLYGRPL
CEEEEHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
ETLLATWGISLMLIQLVRLVFGAQNVEVANPAWLSGGIQVLPNLVLPYNRIVIIAFALFV
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCHHHHHHHHCCHHHHHHHHHHHHH
VVLTWLLLNKTRLGLNVRAVTQNRNMAACCGVPTGRVDMLAFGLGSGIAGLGGVALSQIG
HHHHHHHHHHHHCCCEEEEEECCCCEEEECCCCCCCHHHHHHHHCCCCHHHHHHHHHHHC
NVGPDLGQSYIIDSFLVVVLGGVGQLAGSVFAAFGLGIANKILEPQIGAVLGKILILALI
CCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
ILFIQKRPQGLFALKGRVID
HHHHHCCCCCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043; 2509433 [H]