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The map label for this gene is ureF

Identifier: 77456790

GI number: 77456790

Start: 655958

End: 656632

Strand: Reverse

Name: ureF

Synonym: Pfl01_0562

Alternate gene names: 77456790

Gene position: 656632-655958 (Counterclockwise)

Preceding gene: 77456791

Following gene: 77456789

Centisome position: 10.2

GC content: 66.52

Gene sequence:

>675_bases
GTGAACCCGGCCTGGGCGCTGCTGCGCCTGGCCAGTCCGCAATTGCCGATTGGCGGCTACAGCTATTCCCAGGGCCTGGA
AATGGCTGTGGATAACGGCCGTGTCGACAGCCCGGACAGCGCCCGCCGCTGGATCAGCGATCAATTGCTGCTCAACCTCG
CGCGGTTTGAAGCGCCGCTGCTGCTCGCCCATTGCCAGGCCGCCGCGGACGAACACTGGGATGAATTGCGCAAGCTCTGC
GAAAGCCACCGCGCCAGCCGCGAAACCCGCGAGCTGCATCTGGAGAGCCGGCAGATGGGCTATTCGTTGCAGCAATTGCT
CAACGGCCTGCCCGAACTCGATCAGCCAGCGCGGGACTTCCTCGAACACTGCGCCGAACCGCACCTGGCCCTGTGCTGGG
CACTGGCCGCGCGCGCCTGGCAGATCAGCCCGCAGGACGCCCTCGCCGCGTGGCTGTGGAGCTGGCTGGAAAACCAGCTA
GCGGTGCTGATGAAAACCCTGCCGCTGGGCCAGCAAGCCGCTCAGCGCCTGACCAGCGAACTGCTGCCGCTGCTGCAACA
GGCCCAGCAGGACGCCACCCGAATCAATCCCGAACACCTCGGCAGCGCCGCGTTCGGTCTGTCCCTGGCGTGCATGGCCC
ATGAGCGCCAGTACAGCCGCCTCTTCCGTTCCTAG

Upstream 100 bases:

>100_bases
AGCCGGAACACGGCGCCTACGGTGGCGGCCATCATCATTCGCGGCACGGTGACGAAGACTTCAACTACGCGCCGAAACTC
CATCAGTTCGGCGTCCGTTT

Downstream 100 bases:

>100_bases
GGCCTTTTATCAGGAGAATCACATGAACACACAACCCCTGCGCGTCGGCATCGGCGGCCCGGTCGGTTCCGGCAAGACCG
CGTTGACCCTGGCCCTGTGC

Product: urease accessory protein UreF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 224; Mature: 224

Protein sequence:

>224_residues
MNPAWALLRLASPQLPIGGYSYSQGLEMAVDNGRVDSPDSARRWISDQLLLNLARFEAPLLLAHCQAAADEHWDELRKLC
ESHRASRETRELHLESRQMGYSLQQLLNGLPELDQPARDFLEHCAEPHLALCWALAARAWQISPQDALAAWLWSWLENQL
AVLMKTLPLGQQAAQRLTSELLPLLQQAQQDATRINPEHLGSAAFGLSLACMAHERQYSRLFRS

Sequences:

>Translated_224_residues
MNPAWALLRLASPQLPIGGYSYSQGLEMAVDNGRVDSPDSARRWISDQLLLNLARFEAPLLLAHCQAAADEHWDELRKLC
ESHRASRETRELHLESRQMGYSLQQLLNGLPELDQPARDFLEHCAEPHLALCWALAARAWQISPQDALAAWLWSWLENQL
AVLMKTLPLGQQAAQRLTSELLPLLQQAQQDATRINPEHLGSAAFGLSLACMAHERQYSRLFRS
>Mature_224_residues
MNPAWALLRLASPQLPIGGYSYSQGLEMAVDNGRVDSPDSARRWISDQLLLNLARFEAPLLLAHCQAAADEHWDELRKLC
ESHRASRETRELHLESRQMGYSLQQLLNGLPELDQPARDFLEHCAEPHLALCWALAARAWQISPQDALAAWLWSWLENQL
AVLMKTLPLGQQAAQRLTSELLPLLQQAQQDATRINPEHLGSAAFGLSLACMAHERQYSRLFRS

Specific function: Required for maturation of urease via the functional incorporation of the urease nickel metallocenter

COG id: COG0830

COG function: function code O; Urease accessory protein UreF

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ureF family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): UREF_PSEPF (Q3KIV0)

Other databases:

- EMBL:   CP000094
- RefSeq:   YP_346295.1
- STRING:   Q3KIV0
- GeneID:   3712245
- GenomeReviews:   CP000094_GR
- KEGG:   pfo:Pfl01_0562
- eggNOG:   COG0830
- HOGENOM:   HBG709295
- OMA:   SPQLPIG
- ProtClustDB:   CLSK868970
- BioCyc:   PFLU205922:PFL_0562-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01385
- InterPro:   IPR002639
- PIRSF:   PIRSF009467

Pfam domain/function: PF01730 UreF

EC number: NA

Molecular weight: Translated: 25293; Mature: 25293

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPAWALLRLASPQLPIGGYSYSQGLEMAVDNGRVDSPDSARRWISDQLLLNLARFEAPL
CCHHHHHHHHCCCCCCCCCCCHHCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LLAHCQAAADEHWDELRKLCESHRASRETRELHLESRQMGYSLQQLLNGLPELDQPARDF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
LEHCAEPHLALCWALAARAWQISPQDALAAWLWSWLENQLAVLMKTLPLGQQAAQRLTSE
HHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
LLPLLQQAQQDATRINPEHLGSAAFGLSLACMAHERQYSRLFRS
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNPAWALLRLASPQLPIGGYSYSQGLEMAVDNGRVDSPDSARRWISDQLLLNLARFEAPL
CCHHHHHHHHCCCCCCCCCCCHHCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LLAHCQAAADEHWDELRKLCESHRASRETRELHLESRQMGYSLQQLLNGLPELDQPARDF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
LEHCAEPHLALCWALAARAWQISPQDALAAWLWSWLENQLAVLMKTLPLGQQAAQRLTSE
HHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
LLPLLQQAQQDATRINPEHLGSAAFGLSLACMAHERQYSRLFRS
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA