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The map label for this gene is rlmB [H]

Identifier: 77456761

GI number: 77456761

Start: 620695

End: 621462

Strand: Direct

Name: rlmB [H]

Synonym: Pfl01_0533

Alternate gene names: 77456761

Gene position: 620695-621462 (Clockwise)

Preceding gene: 77456760

Following gene: 77456762

Centisome position: 9.64

GC content: 64.06

Gene sequence:

>768_bases
ATGAGTCAGTTGGAAAAAATCTACGGCGTTCACGCGGTAGAAGCGTTGCTGCGTCACCACCCTAAACGCGTCAAGCAGAT
CTGGCTGGCTGAAAGCCGCAACGATCCGCGCGTGCAGACGCTGATCGAGCTGGCCAACGAAAACCGGGTTCAGATCGGTC
AGGCCGAGCGCCGCGAAATGGACGCCTGGGTCGAAGGCGTGCACCAGGGCGTGGTGGCGGACGTCAGTCCGAGCCAGGTC
TGGGGCGAAGCGATGCTCGACGAGCTGCTTGATCGCACCGAGGGCGCGCCGTTGCTGTTGGTGCTCGACGGCGTGACCGA
CCCGCACAACCTCGGCGCCTGCCTGCGTTCGGCGGATGCTGCCGGTGCGCTGGCGGTCATCGTGCCAAAAGACAAGTCGG
CCACCCTGACCCCTGTGGTGCGTAAAGTGGCTTGCGGCGCGGCGGAAGTGATTCCGCTGGTGGCCGTCACCAACCTTGCG
CGCACCCTGGAAAAGCTTCAGCAGCGCGGGTTGTGGATTGTCGGCACGGCGGGTGAGGCCGAGGTCAGCATCTATGACCA
GGACCTGACCGGCCCGACCATTCTGATCATGGGCGCCGAAGGCAAAGGCATGCGTCGCCTGACCCGCGAGCATTGCGATT
ACCTGGTGCATTTGCCGATGGCCGGTAGCGTCAGCAGTCTCAACGTGTCGGTTGCGACGGGTGTCTGCCTGTTCGAGGCC
CAGCGCCAGCGTGGTGCCAAGGCCAAGGCGGCTGCCAAGAAGGCTTAA

Upstream 100 bases:

>100_bases
GCGGCAAGCCAGCCAAACCGAGCAAACACCGCAAAGGCCCGCCGAAAGCGGGTTCCGCTCCAGCCAAAAGTGGCGGGGCG
CGTAAACCGAAGGCGAAGTC

Downstream 100 bases:

>100_bases
GCCTTACGCAGTCCAAAATGTGGGAGCGAGCCTGCTCGCGAAGCGGAGTTACATTCTGCATCATTGTTGAATGTTAAGCC
GCCATCGCGAGCAGACTCGC

Product: RNA methyltransferase TrmH

Products: NA

Alternate protein names: 23S rRNA Gm2251 2'-O-methyltransferase [H]

Number of amino acids: Translated: 255; Mature: 254

Protein sequence:

>255_residues
MSQLEKIYGVHAVEALLRHHPKRVKQIWLAESRNDPRVQTLIELANENRVQIGQAERREMDAWVEGVHQGVVADVSPSQV
WGEAMLDELLDRTEGAPLLLVLDGVTDPHNLGACLRSADAAGALAVIVPKDKSATLTPVVRKVACGAAEVIPLVAVTNLA
RTLEKLQQRGLWIVGTAGEAEVSIYDQDLTGPTILIMGAEGKGMRRLTREHCDYLVHLPMAGSVSSLNVSVATGVCLFEA
QRQRGAKAKAAAKKA

Sequences:

>Translated_255_residues
MSQLEKIYGVHAVEALLRHHPKRVKQIWLAESRNDPRVQTLIELANENRVQIGQAERREMDAWVEGVHQGVVADVSPSQV
WGEAMLDELLDRTEGAPLLLVLDGVTDPHNLGACLRSADAAGALAVIVPKDKSATLTPVVRKVACGAAEVIPLVAVTNLA
RTLEKLQQRGLWIVGTAGEAEVSIYDQDLTGPTILIMGAEGKGMRRLTREHCDYLVHLPMAGSVSSLNVSVATGVCLFEA
QRQRGAKAKAAAKKA
>Mature_254_residues
SQLEKIYGVHAVEALLRHHPKRVKQIWLAESRNDPRVQTLIELANENRVQIGQAERREMDAWVEGVHQGVVADVSPSQVW
GEAMLDELLDRTEGAPLLLVLDGVTDPHNLGACLRSADAAGALAVIVPKDKSATLTPVVRKVACGAAEVIPLVAVTNLAR
TLEKLQQRGLWIVGTAGEAEVSIYDQDLTGPTILIMGAEGKGMRRLTREHCDYLVHLPMAGSVSSLNVSVATGVCLFEAQ
RQRGAKAKAAAKKA

Specific function: Specifically methylates the ribose of guanosine 2251 in 23S rRNA [H]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. RlmB subfamily [H]

Homologues:

Organism=Homo sapiens, GI40068479, Length=222, Percent_Identity=34.2342342342342, Blast_Score=97, Evalue=1e-20,
Organism=Escherichia coli, GI1790623, Length=241, Percent_Identity=58.0912863070539, Blast_Score=280, Evalue=8e-77,
Organism=Escherichia coli, GI1788935, Length=241, Percent_Identity=26.1410788381743, Blast_Score=85, Evalue=4e-18,
Organism=Escherichia coli, GI1790083, Length=164, Percent_Identity=31.7073170731707, Blast_Score=72, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17555908, Length=254, Percent_Identity=27.1653543307087, Blast_Score=74, Evalue=7e-14,
Organism=Saccharomyces cerevisiae, GI6324775, Length=165, Percent_Identity=33.9393939393939, Blast_Score=91, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004441
- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 27498; Mature: 27367

Theoretical pI: Translated: 7.18; Mature: 7.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQLEKIYGVHAVEALLRHHPKRVKQIWLAESRNDPRVQTLIELANENRVQIGQAERREM
CCHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEECCHHHHHH
DAWVEGVHQGVVADVSPSQVWGEAMLDELLDRTEGAPLLLVLDGVTDPHNLGACLRSADA
HHHHHHHHCCCEECCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHCCCC
AGALAVIVPKDKSATLTPVVRKVACGAAEVIPLVAVTNLARTLEKLQQRGLWIVGTAGEA
CCEEEEEEECCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCC
EVSIYDQDLTGPTILIMGAEGKGMRRLTREHCDYLVHLPMAGSVSSLNVSVATGVCLFEA
EEEEEECCCCCCEEEEECCCCCCHHHHHHHHCCEEEECCCCCCCCCCEEHHHHHHHHHHH
QRQRGAKAKAAAKKA
HHHCCCHHHHHHCCC
>Mature Secondary Structure 
SQLEKIYGVHAVEALLRHHPKRVKQIWLAESRNDPRVQTLIELANENRVQIGQAERREM
CHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEECCHHHHHH
DAWVEGVHQGVVADVSPSQVWGEAMLDELLDRTEGAPLLLVLDGVTDPHNLGACLRSADA
HHHHHHHHCCCEECCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHCCCC
AGALAVIVPKDKSATLTPVVRKVACGAAEVIPLVAVTNLARTLEKLQQRGLWIVGTAGEA
CCEEEEEEECCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCC
EVSIYDQDLTGPTILIMGAEGKGMRRLTREHCDYLVHLPMAGSVSSLNVSVATGVCLFEA
EEEEEECCCCCCEEEEECCCCCCHHHHHHHHCCEEEECCCCCCCCCCEEHHHHHHHHHHH
QRQRGAKAKAAAKKA
HHHCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12928499 [H]