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The map label for this gene is parE [H]

Identifier: 77456727

GI number: 77456727

Start: 577085

End: 578992

Strand: Direct

Name: parE [H]

Synonym: Pfl01_0499

Alternate gene names: 77456727

Gene position: 577085-578992 (Clockwise)

Preceding gene: 77456726

Following gene: 77456728

Centisome position: 8.96

GC content: 63.16

Gene sequence:

>1908_bases
ATGGCCACTCCCAGCGCTAGCTCCTATAACGCCGACGCCATCGAAGTCCTCTCGGGCCTCGACCCGGTGCGCAAACGCCC
CGGCATGTACACCGACACCAGTCGGCCGAACCACCTTGCCCAGGAAGTCATCGACAACAGTGTCGACGAAGCCCTGGCCG
GCCACGCCAAATCGATTCAGGTCATCCTCCACGCCGACCACTCGCTGGAAGTCTGCGACGACGGCCGTGGCATGCCGGTC
GACATCCACCCGGAAGAGGGGGTGTCTGGCGTTGAACTGATCCTCACCAAGCTCCATGCGGGCGGCAAGTTTTCCAACAA
GAACTACCAGTTCTCCGGCGGTCTGCACGGGGTGGGTATTTCCGTGGTCAACGCCTTGTCGACCCAGGTACGGGTGCGGG
TCAAGCGTGACGGCAACGAATACGAAATGACCTTCGCCGACGGCTTCAAGGCCACCGATCTGCAAGTGATCGGCACCGTC
GGCAAGCGCAATACCGGGACCAGCGTGTACTTCGCGCCGGACCCGAAATACTTTGACTCACCAAAATTCTCCATCAGCCG
CCTCAAGCATGTGCTCAAGGCCAAGGCCGTTCTGTGCCCGGGGCTGCTGATCAGCTTCGAAGACAAAGGCACCGGCGAAA
AGGTCGAGTGGCATTACGAGGATGGCTTGCGCTCCTATCTGGTGGACGCGGTCAGCGAATTCGAGCGTCTGCCGGATGCC
CCGTTCTGCGGCAACCTGGCCGGCACCAAGGAAGCGGTCGAGTGGGCGCTGCTGTGGCTGCCGGAAGGCGGCACCTCGGT
CACTGAAAGCTACGTCAACCTGATCCCGACGGAGCAGGGCGGCACCCACGTCAACGGTCTGCGTCAGGGCCTGCTCGATG
CGATGCGCGAATTCTGCGAGTTCCGCAGCCTGCTGCCGCGCGGCGTGAAGCTGGCGCCGGAAGACGTCTGGGAGCGCATT
GCTTTCGTCCTGTCGATGAAGATGCAGGAACCGCAATTCTCCGGCCAGACCAAAGAGCGCCTGTCATCCCGTGAAGCGGC
GGCTTTCGTTTCCGGTGTGGTCAAGGACGCGTTCAGCCTGTGGCTCAACGCCAACCCGGAAACCGGTCTGGCCCTGGCAG
AGCTGGCGATCAGCAACGCCGGCCGTCGTCTGAAGGCCAGCAAAAAAGTCGAGCGCAAACGCATCACCCAGGGACCGGCA
TTGCCGGGCAAACTGGCCGACTGTGCCGGGCAGGACCCGATGCGTTCCGAGCTGTTCCTGGTGGAAGGTGATTCCGCCGG
CGGTTCGGCCAAACAGGCGCGGGACAAGGAGTTCCAGGCGATCCTGCCGTTGCGCGGCAAGATCCTCAACACCTGGGAAG
TCGACGGCAGCGAAGTGCTGGCCAGCCAGGAAGTGCACAACATCGCGGTGGCCATCGGGGTCGATCCGGGCGCCGAGGAC
ATGAGCCAGCTGCGCTACGGCAAGATTTGCATCCTCGCCGACGCCGACTCCGACGGCCTGCACATCGCGACCCTGCTCTG
TGCCTTGTTCGTCCAGCATTTCCGCCCGCTGGTGGATGCCGGTCACGTCTATGTCGCGATGCCGCCGTTGTACCGCATCG
ACCTGGGCAAAGAGATCTACTACGCCCTCGACGAAGCCGAGCGCGACGGGATCCTCGACCGTCTGGTCGCCGAGAAGAAA
CGCGGCAAGCCGCAGGTCACCCGATTCAAAGGCCTGGGTGAAATGAACCCGCCGCAACTGCGCGAAACCACCATGGACCC
GAACACCCGGCGCCTGGTGCAGTTGACCCTCGGGGGCGACTTCGCCGAGACCTCGGAAATGATGGACATGCTGCTGGCGA
AAAAACGCGCCGGCGACCGCAAGACCTGGCTCGAATCCAAGGGTAACCTCGCCGAGGTTCTGGCCTGA

Upstream 100 bases:

>100_bases
CAGCTTTGCCGGGATTGGCGCCGATACCTATCAGGCAATCGATTTCACCGCACTGTGAAGTCTTGCCCCATTTTCATTGA
ATCACTGACGACGAGACCCT

Downstream 100 bases:

>100_bases
TGCGGTTTGGCCGGGCCTTGGCGGGTGCGTTGCTGCTGGGTTCGATGACGGTGTCGGCGGAGCCTGCGCAAGAACTGCGC
GTGCTCGCCGAACACCCGGT

Product: DNA topoisomerase IV subunit B

Products: NA

Alternate protein names: Topoisomerase IV subunit B [H]

Number of amino acids: Translated: 635; Mature: 634

Protein sequence:

>635_residues
MATPSASSYNADAIEVLSGLDPVRKRPGMYTDTSRPNHLAQEVIDNSVDEALAGHAKSIQVILHADHSLEVCDDGRGMPV
DIHPEEGVSGVELILTKLHAGGKFSNKNYQFSGGLHGVGISVVNALSTQVRVRVKRDGNEYEMTFADGFKATDLQVIGTV
GKRNTGTSVYFAPDPKYFDSPKFSISRLKHVLKAKAVLCPGLLISFEDKGTGEKVEWHYEDGLRSYLVDAVSEFERLPDA
PFCGNLAGTKEAVEWALLWLPEGGTSVTESYVNLIPTEQGGTHVNGLRQGLLDAMREFCEFRSLLPRGVKLAPEDVWERI
AFVLSMKMQEPQFSGQTKERLSSREAAAFVSGVVKDAFSLWLNANPETGLALAELAISNAGRRLKASKKVERKRITQGPA
LPGKLADCAGQDPMRSELFLVEGDSAGGSAKQARDKEFQAILPLRGKILNTWEVDGSEVLASQEVHNIAVAIGVDPGAED
MSQLRYGKICILADADSDGLHIATLLCALFVQHFRPLVDAGHVYVAMPPLYRIDLGKEIYYALDEAERDGILDRLVAEKK
RGKPQVTRFKGLGEMNPPQLRETTMDPNTRRLVQLTLGGDFAETSEMMDMLLAKKRAGDRKTWLESKGNLAEVLA

Sequences:

>Translated_635_residues
MATPSASSYNADAIEVLSGLDPVRKRPGMYTDTSRPNHLAQEVIDNSVDEALAGHAKSIQVILHADHSLEVCDDGRGMPV
DIHPEEGVSGVELILTKLHAGGKFSNKNYQFSGGLHGVGISVVNALSTQVRVRVKRDGNEYEMTFADGFKATDLQVIGTV
GKRNTGTSVYFAPDPKYFDSPKFSISRLKHVLKAKAVLCPGLLISFEDKGTGEKVEWHYEDGLRSYLVDAVSEFERLPDA
PFCGNLAGTKEAVEWALLWLPEGGTSVTESYVNLIPTEQGGTHVNGLRQGLLDAMREFCEFRSLLPRGVKLAPEDVWERI
AFVLSMKMQEPQFSGQTKERLSSREAAAFVSGVVKDAFSLWLNANPETGLALAELAISNAGRRLKASKKVERKRITQGPA
LPGKLADCAGQDPMRSELFLVEGDSAGGSAKQARDKEFQAILPLRGKILNTWEVDGSEVLASQEVHNIAVAIGVDPGAED
MSQLRYGKICILADADSDGLHIATLLCALFVQHFRPLVDAGHVYVAMPPLYRIDLGKEIYYALDEAERDGILDRLVAEKK
RGKPQVTRFKGLGEMNPPQLRETTMDPNTRRLVQLTLGGDFAETSEMMDMLLAKKRAGDRKTWLESKGNLAEVLA
>Mature_634_residues
ATPSASSYNADAIEVLSGLDPVRKRPGMYTDTSRPNHLAQEVIDNSVDEALAGHAKSIQVILHADHSLEVCDDGRGMPVD
IHPEEGVSGVELILTKLHAGGKFSNKNYQFSGGLHGVGISVVNALSTQVRVRVKRDGNEYEMTFADGFKATDLQVIGTVG
KRNTGTSVYFAPDPKYFDSPKFSISRLKHVLKAKAVLCPGLLISFEDKGTGEKVEWHYEDGLRSYLVDAVSEFERLPDAP
FCGNLAGTKEAVEWALLWLPEGGTSVTESYVNLIPTEQGGTHVNGLRQGLLDAMREFCEFRSLLPRGVKLAPEDVWERIA
FVLSMKMQEPQFSGQTKERLSSREAAAFVSGVVKDAFSLWLNANPETGLALAELAISNAGRRLKASKKVERKRITQGPAL
PGKLADCAGQDPMRSELFLVEGDSAGGSAKQARDKEFQAILPLRGKILNTWEVDGSEVLASQEVHNIAVAIGVDPGAEDM
SQLRYGKICILADADSDGLHIATLLCALFVQHFRPLVDAGHVYVAMPPLYRIDLGKEIYYALDEAERDGILDRLVAEKKR
GKPQVTRFKGLGEMNPPQLRETTMDPNTRRLVQLTLGGDFAETSEMMDMLLAKKRAGDRKTWLESKGNLAEVLA

Specific function: Topoisomerase IV is essential for chromosome segregation. It relaxes supercoiled DNA. Performs the decatenation events required during the replication of a circular DNA molecule [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type II topoisomerase family [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=624, Percent_Identity=24.0384615384615, Blast_Score=132, Evalue=8e-31,
Organism=Homo sapiens, GI19913406, Length=636, Percent_Identity=24.685534591195, Blast_Score=125, Evalue=1e-28,
Organism=Escherichia coli, GI1789408, Length=629, Percent_Identity=73.449920508744, Blast_Score=975, Evalue=0.0,
Organism=Escherichia coli, GI48994957, Length=557, Percent_Identity=40.394973070018, Blast_Score=375, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI17535065, Length=625, Percent_Identity=23.36, Blast_Score=124, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI212645845, Length=605, Percent_Identity=23.1404958677686, Blast_Score=115, Evalue=5e-26,
Organism=Saccharomyces cerevisiae, GI6324241, Length=683, Percent_Identity=24.1581259150805, Blast_Score=141, Evalue=3e-34,
Organism=Drosophila melanogaster, GI17136538, Length=630, Percent_Identity=22.0634920634921, Blast_Score=125, Evalue=7e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR005737 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c [H]

EC number: 5.99.1.-

Molecular weight: Translated: 69462; Mature: 69331

Theoretical pI: Translated: 5.94; Mature: 5.94

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATPSASSYNADAIEVLSGLDPVRKRPGMYTDTSRPNHLAQEVIDNSVDEALAGHAKSIQ
CCCCCCCCCCCHHHHHHHCCCHHHHCCCCCCCCCCCHHHHHHHHHCHHHHHHHCCCCEEE
VILHADHSLEVCDDGRGMPVDIHPEEGVSGVELILTKLHAGGKFSNKNYQFSGGLHGVGI
EEEECCCCEEECCCCCCCCEEECCCCCCCHHHHHHHHHHCCCCCCCCCEEECCCCCHHHH
SVVNALSTQVRVRVKRDGNEYEMTFADGFKATDLQVIGTVGKRNTGTSVYFAPDPKYFDS
HHHHHHHHEEEEEEEECCCEEEEEEECCCCCCCEEEEECCCCCCCCCEEEECCCCCCCCC
PKFSISRLKHVLKAKAVLCPGLLISFEDKGTGEKVEWHYEDGLRSYLVDAVSEFERLPDA
CCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCC
PFCGNLAGTKEAVEWALLWLPEGGTSVTESYVNLIPTEQGGTHVNGLRQGLLDAMREFCE
CCCCCCCCCHHHHEEEEEEECCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
FRSLLPRGVKLAPEDVWERIAFVLSMKMQEPQFSGQTKERLSSREAAAFVSGVVKDAFSL
HHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
WLNANPETGLALAELAISNAGRRLKASKKVERKRITQGPALPGKLADCAGQDPMRSELFL
HCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCCCEEE
VEGDSAGGSAKQARDKEFQAILPLRGKILNTWEVDGSEVLASQEVHNIAVAIGVDPGAED
EECCCCCCCHHHHHCCHHHEECCCCCCEEEEEECCHHHHHHHHHHCEEEEEEECCCCHHH
MSQLRYGKICILADADSDGLHIATLLCALFVQHFRPLVDAGHVYVAMPPLYRIDLGKEIY
HHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCEEEEECCCHHH
YALDEAERDGILDRLVAEKKRGKPQVTRFKGLGEMNPPQLRETTMDPNTRRLVQLTLGGD
HHHHHHHHCHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCHHHCCCCCCCCEEEEEEECCC
FAETSEMMDMLLAKKRAGDRKTWLESKGNLAEVLA
HHHHHHHHHHHHHHHCCCCHHHHHHCCCCHHHHCC
>Mature Secondary Structure 
ATPSASSYNADAIEVLSGLDPVRKRPGMYTDTSRPNHLAQEVIDNSVDEALAGHAKSIQ
CCCCCCCCCCHHHHHHHCCCHHHHCCCCCCCCCCCHHHHHHHHHCHHHHHHHCCCCEEE
VILHADHSLEVCDDGRGMPVDIHPEEGVSGVELILTKLHAGGKFSNKNYQFSGGLHGVGI
EEEECCCCEEECCCCCCCCEEECCCCCCCHHHHHHHHHHCCCCCCCCCEEECCCCCHHHH
SVVNALSTQVRVRVKRDGNEYEMTFADGFKATDLQVIGTVGKRNTGTSVYFAPDPKYFDS
HHHHHHHHEEEEEEEECCCEEEEEEECCCCCCCEEEEECCCCCCCCCEEEECCCCCCCCC
PKFSISRLKHVLKAKAVLCPGLLISFEDKGTGEKVEWHYEDGLRSYLVDAVSEFERLPDA
CCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCC
PFCGNLAGTKEAVEWALLWLPEGGTSVTESYVNLIPTEQGGTHVNGLRQGLLDAMREFCE
CCCCCCCCCHHHHEEEEEEECCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
FRSLLPRGVKLAPEDVWERIAFVLSMKMQEPQFSGQTKERLSSREAAAFVSGVVKDAFSL
HHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
WLNANPETGLALAELAISNAGRRLKASKKVERKRITQGPALPGKLADCAGQDPMRSELFL
HCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCCCEEE
VEGDSAGGSAKQARDKEFQAILPLRGKILNTWEVDGSEVLASQEVHNIAVAIGVDPGAED
EECCCCCCCHHHHHCCHHHEECCCCCCEEEEEECCHHHHHHHHHHCEEEEEEECCCCHHH
MSQLRYGKICILADADSDGLHIATLLCALFVQHFRPLVDAGHVYVAMPPLYRIDLGKEIY
HHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCEEEEECCCHHH
YALDEAERDGILDRLVAEKKRGKPQVTRFKGLGEMNPPQLRETTMDPNTRRLVQLTLGGD
HHHHHHHHCHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCHHHCCCCCCCCEEEEEEECCC
FAETSEMMDMLLAKKRAGDRKTWLESKGNLAEVLA
HHHHHHHHHHHHHHHCCCCHHHHHHCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]