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The map label for this gene is nudF [H]

Identifier: 77456723

GI number: 77456723

Start: 574356

End: 574973

Strand: Direct

Name: nudF [H]

Synonym: Pfl01_0495

Alternate gene names: 77456723

Gene position: 574356-574973 (Clockwise)

Preceding gene: 77456721

Following gene: 77456724

Centisome position: 8.92

GC content: 60.19

Gene sequence:

>618_bases
ATGACCGATTTTGCCAAAGCCATTCCGACCGCCGTCGATATCGTGCGACGCGAACAGTGCTACAAGGGCTTCTACAAGCT
CGACCGTCTGCACTTGCGCCACGAACTGTTCGCCGGTGGCATGAGCCGCGAAATCAATCGTGAAGTGTTCGTGCGCCACG
ATGCCGTGTGCATGTTGCCCTACGATCCGCAGCGCGATGAAGTGGTGCTGATCGAGCAGTTTCGCGTCGGTGCTCTGGGC
AAGACGGACAACCCGTGGCTGGTGGAGCTGGTCGCCGGTCTGATCGACAAGGACGAACAGCCGGAAGAAGTTGCTCACCG
CGAGGCACAGGAGGAAGCTGGGCTGGACATCAAGGCCCTGTGGCCGATGACCAAATACTTTCCGTCGCCGGGCGGCAGCA
ACGAATTCGTACATTTGTACCTGGGGCGTTGCAGCACCGAAGGCGCTGGCGGCCTGCATGGGCTGGAGGAAGAAGCCGAA
GATATTCGCGTCACGGTCTGGGCTTTTGAAGATGCCCTGCAGGCCGTACGTGACGGACGGATTGCCAATGCGGCGAGCAT
CATCGCCTTGCAGTGGCTGGCGCTCAATCGCGCCGAAGTGAGGGGGCTATGGTCGTAA

Upstream 100 bases:

>100_bases
TAAAGGGGCTGCATCAAACATCACGGGCGCGTTAGGATGGCGCGAAGTCGAGGTCGCAGGTAAGGCGCCTCGTATTGGAT
TTGCAGTAAAGAGGATGCTC

Downstream 100 bases:

>100_bases
ACAAGTTGCGCGATCGCTACCGCGTCGACCTCGTGGGCCTGCAAGCCGCCTGCGAGGCGAACTACGCGCGCCTGATGCGA
CTGTTGCCGGACATGCGCAG

Product: nucleoside diphosphate pyrophosphatase

Products: NA

Alternate protein names: ADP-ribose diphosphatase; ADP-ribose phosphohydrolase; ASPPase; Adenosine diphosphoribose pyrophosphatase; ADPR-PPase [H]

Number of amino acids: Translated: 205; Mature: 204

Protein sequence:

>205_residues
MTDFAKAIPTAVDIVRREQCYKGFYKLDRLHLRHELFAGGMSREINREVFVRHDAVCMLPYDPQRDEVVLIEQFRVGALG
KTDNPWLVELVAGLIDKDEQPEEVAHREAQEEAGLDIKALWPMTKYFPSPGGSNEFVHLYLGRCSTEGAGGLHGLEEEAE
DIRVTVWAFEDALQAVRDGRIANAASIIALQWLALNRAEVRGLWS

Sequences:

>Translated_205_residues
MTDFAKAIPTAVDIVRREQCYKGFYKLDRLHLRHELFAGGMSREINREVFVRHDAVCMLPYDPQRDEVVLIEQFRVGALG
KTDNPWLVELVAGLIDKDEQPEEVAHREAQEEAGLDIKALWPMTKYFPSPGGSNEFVHLYLGRCSTEGAGGLHGLEEEAE
DIRVTVWAFEDALQAVRDGRIANAASIIALQWLALNRAEVRGLWS
>Mature_204_residues
TDFAKAIPTAVDIVRREQCYKGFYKLDRLHLRHELFAGGMSREINREVFVRHDAVCMLPYDPQRDEVVLIEQFRVGALGK
TDNPWLVELVAGLIDKDEQPEEVAHREAQEEAGLDIKALWPMTKYFPSPGGSNEFVHLYLGRCSTEGAGGLHGLEEEAED
IRVTVWAFEDALQAVRDGRIANAASIIALQWLALNRAEVRGLWS

Specific function: Acts on ADP-mannose and ADP-glucose as well as ADP- ribose. Prevents glycogen biosynthesis. The reaction catalyzed by this enzyme is a limiting step of the gluconeogenic process [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 nudix hydrolase domain [H]

Homologues:

Organism=Escherichia coli, GI1789412, Length=194, Percent_Identity=48.9690721649485, Blast_Score=193, Evalue=6e-51,
Organism=Escherichia coli, GI1788810, Length=151, Percent_Identity=34.4370860927152, Blast_Score=86, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004385
- InterPro:   IPR020084
- InterPro:   IPR000086
- InterPro:   IPR015797 [H]

Pfam domain/function: PF00293 NUDIX [H]

EC number: =3.6.1.13 [H]

Molecular weight: Translated: 23148; Mature: 23016

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS00893 NUDIX

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDFAKAIPTAVDIVRREQCYKGFYKLDRLHLRHELFAGGMSREINREVFVRHDAVCMLP
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCHHEEEEECCEEEEC
YDPQRDEVVLIEQFRVGALGKTDNPWLVELVAGLIDKDEQPEEVAHREAQEEAGLDIKAL
CCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCEEEE
WPMTKYFPSPGGSNEFVHLYLGRCSTEGAGGLHGLEEEAEDIRVTVWAFEDALQAVRDGR
CCHHHHCCCCCCCCCEEEEEEECCCCCCCCCCCCCHHCCCCEEEEEEEHHHHHHHHHCCC
IANAASIIALQWLALNRAEVRGLWS
CCCHHHHHHHHHHHHCHHHHCCCCC
>Mature Secondary Structure 
TDFAKAIPTAVDIVRREQCYKGFYKLDRLHLRHELFAGGMSREINREVFVRHDAVCMLP
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCHHEEEEECCEEEEC
YDPQRDEVVLIEQFRVGALGKTDNPWLVELVAGLIDKDEQPEEVAHREAQEEAGLDIKAL
CCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCEEEE
WPMTKYFPSPGGSNEFVHLYLGRCSTEGAGGLHGLEEEAEDIRVTVWAFEDALQAVRDGR
CCHHHHCCCCCCCCCEEEEEEECCCCCCCCCCCCCHHCCCCEEEEEEEHHHHHHHHHCCC
IANAASIIALQWLALNRAEVRGLWS
CCCHHHHHHHHHHHHCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]