Click here to switch to the map view.

The map label for this gene is thiI

Identifier: 77456574

GI number: 77456574

Start: 389897

End: 391351

Strand: Direct

Name: thiI

Synonym: Pfl01_0346

Alternate gene names: 77456574

Gene position: 389897-391351 (Clockwise)

Preceding gene: 77456573

Following gene: 77456575

Centisome position: 6.06

GC content: 59.11

Gene sequence:

>1455_bases
ATGAAACTAATCGTAAAAGTCTTCCCCGAGATCACCATCAAAAGCCGACCTGTCCGGACGAAATTCATCCGCCAGCTGGC
CAAGAACATCCGCACCGTGCTCCGCGACCTGGACCCGGCCGTGGTGGTGAACGGTGTGTGGGACAATCTCGAGCTGGAAA
CCCGCGTCACCGATCCCAAGGCCCTGAAAGAGATGGGCGAACGCCTGACCTGCATGCCGGGCATCGCGCACTTTCTGCAG
ATCGACGAGTACCCGCTGGGTGACTTCGACGACATCACCGAGAAGTGCAAACAGCACTACGGCGATGCGCTGGCCGGGAA
GATCTTCTCGGTGCGCTGCAAGCGTGCGGGCAAGCATGCGTTCAGCTCGATGGACGTCGAAAAATACGTCGGCAGCAAGC
TGCGCCGTGAGTGCGGTGCCGCCGGAATCGACCTGAAACAGCCGGAAATCGAAGTCCGTATCGAAGTTCGCGACAAACGG
TTGTTTGTGATCCACAGCCAGCACAATGGTATCGGCGGTTACCCGCTCGGTGCGCTGGAACAGACGCTGGTGCTGATGTC
AGGCGGCTTTGACTCGACGGTTGCCGCGTACCAGATCCTGCGTCGCGGCCTGATGACGCACTTCTGCTTCTTCAATCTGG
GCGGTCGTGCCCACGAATTGGGCGTGATGGAAGTCGCGCATTTCATCTGGAAGAAGTACGGCAGCTCGCAACGCGTGCTA
TTTGTCAGTGTTCCGTTCGAAGAAGTATTGGGCGAAATTCTCGGCAAGGTCGACGACAGTCATATGGGCGTCGTATTGAA
GCGTATGATGTTGCGCGCAGCGTCGAGCATTGCCGAACGCCTGCACATCGATGCGCTGGTCACCGGCGAAGCGATTTCCC
AGGTGTCGAGCCAGACGTTGCCGAACCTGTCGGTCATCGATTGCGTCACCGACAAGCTGGTGCTGCGACCGCTGATCGTC
GCCCACAAGCAGGACATCATCGACACGGCCGACCAGATCGGTACCGGTGATTTCGCCCGGCACATGCCGGAATACTGCGG
CGTCATCTCGGTCAACCCGAAGACCGCCGCCAAGCGTGGCCGGGTCGAGCACGAAGAGCAGGAATTCGACATGGCGGTGC
TCGAGCGTGCGCTCGCAAACGCCAGACTGGTGCCGATCGATCGGGTGATCGACGAATTGGGCCAGGATTTGCAGATCGAA
GAAGTCAGCGAAGCACTGGCCGGTCAGATCGTCATCGACATCCGCCACCCGGATGCCGCCGAGGATGACCCGCTGGAACT
CGCCGGCATAGAGGTACAGACGATGCCGTTCTACGCAGTGAACGCTCGTTTCAAGGAACTGGACCCTACTCGCCAGTACC
TGCTGTATTGCGACAAAGGCGTGATGAGTCGCCTGCATGCTCACCATTTGCTCAGTGAGGGGCATGCCAATGTGCGCGTT
TATCGACCGAGCTAA

Upstream 100 bases:

>100_bases
TGCGTGCAAAACCTTCTGCACATTAACTGGTTAAACCTTGAGCAATTTCCGCTATAATCCGCGCCCCCCTTTTTCGGCTG
GCCCTGCGCGCGCTGTTTTC

Downstream 100 bases:

>100_bases
GTGCCCGGGGCTGTTTGCCTGTGGCCTGCGTCACCGGCCCCCCGACACCGCCGTCAAGCTGTAACGGCCATGCCGGACAC
TACTGTCAATCGCTGCCAAG

Product: thiamine biosynthesis protein ThiI

Products: NA

Alternate protein names: Sulfur carrier protein ThiS sulfurtransferase; Thiamine biosynthesis protein thiI; tRNA 4-thiouridine synthase

Number of amino acids: Translated: 484; Mature: 484

Protein sequence:

>484_residues
MKLIVKVFPEITIKSRPVRTKFIRQLAKNIRTVLRDLDPAVVVNGVWDNLELETRVTDPKALKEMGERLTCMPGIAHFLQ
IDEYPLGDFDDITEKCKQHYGDALAGKIFSVRCKRAGKHAFSSMDVEKYVGSKLRRECGAAGIDLKQPEIEVRIEVRDKR
LFVIHSQHNGIGGYPLGALEQTLVLMSGGFDSTVAAYQILRRGLMTHFCFFNLGGRAHELGVMEVAHFIWKKYGSSQRVL
FVSVPFEEVLGEILGKVDDSHMGVVLKRMMLRAASSIAERLHIDALVTGEAISQVSSQTLPNLSVIDCVTDKLVLRPLIV
AHKQDIIDTADQIGTGDFARHMPEYCGVISVNPKTAAKRGRVEHEEQEFDMAVLERALANARLVPIDRVIDELGQDLQIE
EVSEALAGQIVIDIRHPDAAEDDPLELAGIEVQTMPFYAVNARFKELDPTRQYLLYCDKGVMSRLHAHHLLSEGHANVRV
YRPS

Sequences:

>Translated_484_residues
MKLIVKVFPEITIKSRPVRTKFIRQLAKNIRTVLRDLDPAVVVNGVWDNLELETRVTDPKALKEMGERLTCMPGIAHFLQ
IDEYPLGDFDDITEKCKQHYGDALAGKIFSVRCKRAGKHAFSSMDVEKYVGSKLRRECGAAGIDLKQPEIEVRIEVRDKR
LFVIHSQHNGIGGYPLGALEQTLVLMSGGFDSTVAAYQILRRGLMTHFCFFNLGGRAHELGVMEVAHFIWKKYGSSQRVL
FVSVPFEEVLGEILGKVDDSHMGVVLKRMMLRAASSIAERLHIDALVTGEAISQVSSQTLPNLSVIDCVTDKLVLRPLIV
AHKQDIIDTADQIGTGDFARHMPEYCGVISVNPKTAAKRGRVEHEEQEFDMAVLERALANARLVPIDRVIDELGQDLQIE
EVSEALAGQIVIDIRHPDAAEDDPLELAGIEVQTMPFYAVNARFKELDPTRQYLLYCDKGVMSRLHAHHLLSEGHANVRV
YRPS
>Mature_484_residues
MKLIVKVFPEITIKSRPVRTKFIRQLAKNIRTVLRDLDPAVVVNGVWDNLELETRVTDPKALKEMGERLTCMPGIAHFLQ
IDEYPLGDFDDITEKCKQHYGDALAGKIFSVRCKRAGKHAFSSMDVEKYVGSKLRRECGAAGIDLKQPEIEVRIEVRDKR
LFVIHSQHNGIGGYPLGALEQTLVLMSGGFDSTVAAYQILRRGLMTHFCFFNLGGRAHELGVMEVAHFIWKKYGSSQRVL
FVSVPFEEVLGEILGKVDDSHMGVVLKRMMLRAASSIAERLHIDALVTGEAISQVSSQTLPNLSVIDCVTDKLVLRPLIV
AHKQDIIDTADQIGTGDFARHMPEYCGVISVNPKTAAKRGRVEHEEQEFDMAVLERALANARLVPIDRVIDELGQDLQIE
EVSEALAGQIVIDIRHPDAAEDDPLELAGIEVQTMPFYAVNARFKELDPTRQYLLYCDKGVMSRLHAHHLLSEGHANVRV
YRPS

Specific function: Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. Thi

COG id: COG0301

COG function: function code H; Thiamine biosynthesis ATP pyrophosphatase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain

Homologues:

Organism=Escherichia coli, GI1786625, Length=484, Percent_Identity=49.5867768595041, Blast_Score=486, Evalue=1e-138,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THII_PSEPF (Q3KJG6)

Other databases:

- EMBL:   CP000094
- RefSeq:   YP_346079.1
- HSSP:   Q81KU0
- ProteinModelPortal:   Q3KJG6
- SMR:   Q3KJG6
- STRING:   Q3KJG6
- GeneID:   3713374
- GenomeReviews:   CP000094_GR
- KEGG:   pfo:Pfl01_0346
- eggNOG:   COG0301
- HOGENOM:   HBG646072
- OMA:   GYPIGTQ
- ProtClustDB:   PRK01269
- BioCyc:   PFLU205922:PFL_0346-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00021
- InterPro:   IPR001763
- InterPro:   IPR014729
- InterPro:   IPR003720
- InterPro:   IPR020536
- InterPro:   IPR004114
- Gene3D:   G3DSA:3.40.250.10
- Gene3D:   G3DSA:3.40.50.620
- SMART:   SM00981
- TIGRFAMs:   TIGR00342

Pfam domain/function: PF02568 ThiI; PF02926 THUMP; SSF52821 Rhodanese-like

EC number: =2.8.1.4

Molecular weight: Translated: 54193; Mature: 54193

Theoretical pI: Translated: 6.70; Mature: 6.70

Prosite motif: PS50206 RHODANESE_3; PS51165 THUMP

Important sites: ACT_SITE 457-457 BINDING 267-267 BINDING 289-289 BINDING 298-298

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLIVKVFPEITIKSRPVRTKFIRQLAKNIRTVLRDLDPAVVVNGVWDNLELETRVTDPK
CCEEEEECCCCEECCCCHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCEEEEEECCCHH
ALKEMGERLTCMPGIAHFLQIDEYPLGDFDDITEKCKQHYGDALAGKIFSVRCKRAGKHA
HHHHHHHHCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHH
FSSMDVEKYVGSKLRRECGAAGIDLKQPEIEVRIEVRDKRLFVIHSQHNGIGGYPLGALE
HHCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEECCEEEEEEECCCCCCCCCHHHHH
QTLVLMSGGFDSTVAAYQILRRGLMTHFCFFNLGGRAHELGVMEVAHFIWKKYGSSQRVL
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCEEE
FVSVPFEEVLGEILGKVDDSHMGVVLKRMMLRAASSIAERLHIDALVTGEAISQVSSQTL
EEECCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEECHHHHHHHHHHCC
PNLSVIDCVTDKLVLRPLIVAHKQDIIDTADQIGTGDFARHMPEYCGVISVNPKTAAKRG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCEEECCCHHHHHCC
RVEHEEQEFDMAVLERALANARLVPIDRVIDELGQDLQIEEVSEALAGQIVIDIRHPDAA
CCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCHHHHHHHHCCEEEEEEECCCCC
EDDPLELAGIEVQTMPFYAVNARFKELDPTRQYLLYCDKGVMSRLHAHHLLSEGHANVRV
CCCCCEEECEEEEECCEEEECCHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEE
YRPS
ECCC
>Mature Secondary Structure
MKLIVKVFPEITIKSRPVRTKFIRQLAKNIRTVLRDLDPAVVVNGVWDNLELETRVTDPK
CCEEEEECCCCEECCCCHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCEEEEEECCCHH
ALKEMGERLTCMPGIAHFLQIDEYPLGDFDDITEKCKQHYGDALAGKIFSVRCKRAGKHA
HHHHHHHHCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHH
FSSMDVEKYVGSKLRRECGAAGIDLKQPEIEVRIEVRDKRLFVIHSQHNGIGGYPLGALE
HHCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEECCEEEEEEECCCCCCCCCHHHHH
QTLVLMSGGFDSTVAAYQILRRGLMTHFCFFNLGGRAHELGVMEVAHFIWKKYGSSQRVL
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCEEE
FVSVPFEEVLGEILGKVDDSHMGVVLKRMMLRAASSIAERLHIDALVTGEAISQVSSQTL
EEECCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEECHHHHHHHHHHCC
PNLSVIDCVTDKLVLRPLIVAHKQDIIDTADQIGTGDFARHMPEYCGVISVNPKTAAKRG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCEEECCCHHHHHCC
RVEHEEQEFDMAVLERALANARLVPIDRVIDELGQDLQIEEVSEALAGQIVIDIRHPDAA
CCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCHHHHHHHHCCEEEEEEECCCCC
EDDPLELAGIEVQTMPFYAVNARFKELDPTRQYLLYCDKGVMSRLHAHHLLSEGHANVRV
CCCCCEEECEEEEECCEEEECCHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEE
YRPS
ECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA