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The map label for this gene is ycgF [H]

Identifier: 77456492

GI number: 77456492

Start: 311226

End: 312005

Strand: Reverse

Name: ycgF [H]

Synonym: Pfl01_0264

Alternate gene names: 77456492

Gene position: 312005-311226 (Counterclockwise)

Preceding gene: 77456494

Following gene: 77456491

Centisome position: 4.85

GC content: 55.38

Gene sequence:

>780_bases
GTGACCAAATTTCCGTCTTCATTGACCTCACCCCAATCCGGGTGCCAGGGCTGCCAGCAAAGCGAGCCGCTGGGCTTCGA
TTTTTCCTTCGCCTATCAGCCCATCGTGGATCTGCGGGACAAGTCGGTGTACGCCTACGAGGCCCTGGTCCGTGGTACGG
CCGGTGAAGGTGCGTTAACCGTGCTGGATCAAGTTACAGAAGATAACCGCTACCGTTTCGACCAGCTCTGCCGGACTCGC
GCCATCGAAGGCGCAGCCGGGCTTAATATGCAGACACACTTGTCGATCAACTTCATGCCCAACGCCGTCTATCGTCCAGA
GCTGTGCATACGGAGCACGTTGGAAGCAGCAAAAAAACACAATTTCCCGGTCGACCGGCTGATTTTCGAAACACTGGAAA
GCGAGCATGTAGATAACTATCGCCATTTGACCAATATTCTGCGTGAATACCGCGAATTCGGCTTCAAGACGGCCATCGAC
GATTTCGGTGCCGGGTATTCGGGGCTCAATCTGCTGGCCGATTTCCAGCCGGACCTGATCAAACTCGACATGGCGCTGAT
TCGCGATATCGACCATGACCGTGTACGTCAGGCTATCGTTCGGGGGATTGTCACAATCTGTGCGGAGTTGGGCGTTACAG
TCATCGCCGAAGGCATAGAGAGTGCCGGTGAACGGGATTTCCTGGCGGACTGCGGAATATTTCTGATGCAGGGTTACTGG
TTCGCCAGACCCGCATTCAAAGCGTTAGCCCAGGTGTCCCCTGAAGCGTGGACGCGTTAA

Upstream 100 bases:

>100_bases
GCCAGTAGTCGGAACTGTACTACCGATTCTGTCGGGCGCAAGAACTCGGCCCTCCAGATAAACTGTGATCTTCGCCGGTT
CAACGGCATGGAGTGATCGA

Downstream 100 bases:

>100_bases
TCGTTGGTTTTTTCCATTGAAGACATTTGACCATTTGACCGTTATCGGTCTGCGCGAGTGGGTGGCGCTTCCGGATCTGG
GAGTCGCTGGCCTTCGGGCA

Product: diguanylate phosphodiesterase

Products: NA

Alternate protein names: Blrp [H]

Number of amino acids: Translated: 259; Mature: 258

Protein sequence:

>259_residues
MTKFPSSLTSPQSGCQGCQQSEPLGFDFSFAYQPIVDLRDKSVYAYEALVRGTAGEGALTVLDQVTEDNRYRFDQLCRTR
AIEGAAGLNMQTHLSINFMPNAVYRPELCIRSTLEAAKKHNFPVDRLIFETLESEHVDNYRHLTNILREYREFGFKTAID
DFGAGYSGLNLLADFQPDLIKLDMALIRDIDHDRVRQAIVRGIVTICAELGVTVIAEGIESAGERDFLADCGIFLMQGYW
FARPAFKALAQVSPEAWTR

Sequences:

>Translated_259_residues
MTKFPSSLTSPQSGCQGCQQSEPLGFDFSFAYQPIVDLRDKSVYAYEALVRGTAGEGALTVLDQVTEDNRYRFDQLCRTR
AIEGAAGLNMQTHLSINFMPNAVYRPELCIRSTLEAAKKHNFPVDRLIFETLESEHVDNYRHLTNILREYREFGFKTAID
DFGAGYSGLNLLADFQPDLIKLDMALIRDIDHDRVRQAIVRGIVTICAELGVTVIAEGIESAGERDFLADCGIFLMQGYW
FARPAFKALAQVSPEAWTR
>Mature_258_residues
TKFPSSLTSPQSGCQGCQQSEPLGFDFSFAYQPIVDLRDKSVYAYEALVRGTAGEGALTVLDQVTEDNRYRFDQLCRTRA
IEGAAGLNMQTHLSINFMPNAVYRPELCIRSTLEAAKKHNFPVDRLIFETLESEHVDNYRHLTNILREYREFGFKTAIDD
FGAGYSGLNLLADFQPDLIKLDMALIRDIDHDRVRQAIVRGIVTICAELGVTVIAEGIESAGERDFLADCGIFLMQGYWF
ARPAFKALAQVSPEAWTR

Specific function: Binds to and releases the YcgE repressor from its bound DNA target in a blue-light-dependent (470 nm) fashion. A shift to low temperature also triggers a YcgF-medisted relief of repression by YcgE, suggesting YcgF may serve as a thermometer. Blue light ma

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 EAL domain [H]

Homologues:

Organism=Escherichia coli, GI1787410, Length=229, Percent_Identity=29.2576419213974, Blast_Score=110, Evalue=8e-26,
Organism=Escherichia coli, GI1787541, Length=238, Percent_Identity=32.3529411764706, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI226510982, Length=231, Percent_Identity=26.8398268398268, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1788502, Length=229, Percent_Identity=27.0742358078603, Blast_Score=88, Evalue=5e-19,
Organism=Escherichia coli, GI1790496, Length=251, Percent_Identity=25.4980079681275, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI87081845, Length=225, Percent_Identity=28, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI87081980, Length=230, Percent_Identity=26.9565217391304, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI87082096, Length=150, Percent_Identity=29.3333333333333, Blast_Score=81, Evalue=6e-17,
Organism=Escherichia coli, GI1788849, Length=156, Percent_Identity=30.7692307692308, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI87081743, Length=225, Percent_Identity=25.3333333333333, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1787055, Length=228, Percent_Identity=29.8245614035088, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI1786507, Length=139, Percent_Identity=29.4964028776978, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI87081921, Length=237, Percent_Identity=23.6286919831224, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001792
- InterPro:   IPR007024
- InterPro:   IPR001633 [H]

Pfam domain/function: PF04940 BLUF; PF00563 EAL [H]

EC number: NA

Molecular weight: Translated: 29106; Mature: 28975

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS50883 EAL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKFPSSLTSPQSGCQGCQQSEPLGFDFSFAYQPIVDLRDKSVYAYEALVRGTAGEGALT
CCCCCCCCCCCHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHH
VLDQVTEDNRYRFDQLCRTRAIEGAAGLNMQTHLSINFMPNAVYRPELCIRSTLEAAKKH
HHHHHHCCCCHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHC
NFPVDRLIFETLESEHVDNYRHLTNILREYREFGFKTAIDDFGAGYSGLNLLADFQPDLI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHCCCCHHH
KLDMALIRDIDHDRVRQAIVRGIVTICAELGVTVIAEGIESAGERDFLADCGIFLMQGYW
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCHH
FARPAFKALAQVSPEAWTR
HHHHHHHHHHHCCCHHCCC
>Mature Secondary Structure 
TKFPSSLTSPQSGCQGCQQSEPLGFDFSFAYQPIVDLRDKSVYAYEALVRGTAGEGALT
CCCCCCCCCCHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHH
VLDQVTEDNRYRFDQLCRTRAIEGAAGLNMQTHLSINFMPNAVYRPELCIRSTLEAAKKH
HHHHHHCCCCHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHC
NFPVDRLIFETLESEHVDNYRHLTNILREYREFGFKTAIDDFGAGYSGLNLLADFQPDLI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHCCCCHHH
KLDMALIRDIDHDRVRQAIVRGIVTICAELGVTVIAEGIESAGERDFLADCGIFLMQGYW
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCHH
FARPAFKALAQVSPEAWTR
HHHHHHHHHHHCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]