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The map label for this gene is emrA [H]

Identifier: 77456371

GI number: 77456371

Start: 172515

End: 173585

Strand: Direct

Name: emrA [H]

Synonym: Pfl01_0143

Alternate gene names: 77456371

Gene position: 172515-173585 (Clockwise)

Preceding gene: 77456370

Following gene: 77456372

Centisome position: 2.68

GC content: 65.08

Gene sequence:

>1071_bases
ATGACGACTCAAGCAAAGCAAAAACTCGCGGTGGCTGTGGCCGCTGCGCTGGCGGTCGGTGTGCTGGTGTATCTGGCGAT
GCCCGGCCTGTTCGGCAAGCGCACTCAGCAGAACACCAACGACGCGTTCGTCTCCGCCGATTTCACCCTGGTGGTGCCGC
GTGTGGCGGGGTTCATCAAAGAGGTGCTGGTGGAAGACAACCAGCAGGTCAAGGCCGGGCAGTTGCTGGCGCTGATCGAT
GACCGCGACTTGCGCGCCGCTGCCGAAGCCGCCGATGCGCAAACCCTGGTCGCCCGCGCGCAGTTGCAGAACGCCAAGGC
CACCCTTGAGCGCCAGTCCTCGGTGATCGCTCAGGCGCAGGCGTCGGTGGTGTCGGCCAAGGCTGAAATGGCTTTCGCCC
AGCAGGAATTGAACCGCTATAACCACTTGGCTGGCGTCGGTGCAGGCACCGTGCAGAACGCGCAACAGGCGCGCACCCGG
ATCGATCAGGCCTCTGCGCGGCTGGACACGGCCACCGCGAAACTGGCAGCGGAACGCAAGCAGGTGGAAATCCTCACGGC
GCAGCGCGATGCCGCCGAAGGCAGTTTGAAACACGCGCAGGCCGCGCTGGAAATCGCCAGTTTCGAGCTGTCCTACACGC
GCATCACCGCGCCCCAGGACGGCATGATCGGCGAGCGTGCCGTACGGGTCGGCGCCTACGTGACACCGGGTAGCAAGCTG
CTGGCGGTGGTGCCGTTGAAGCAGGCCTATGTGGTCGCCAACTTCCAGGAAACCCAGCTCACCGACGTGCAGCCGGGGCA
GGACGTTGTGGTGCGGGTCGACAGCCTCGGCGGCGAAGCCCTCACCGGCCGCGTCGAGAGCATTGCCCCAGCGACCGGCG
TGACCTTCGCGGCGGTCAAACCGGACAACGCCACCGGCAACTTCACCAAGGTTGTGCAGCGAATTCCGGTGAAGGTTCTG
CTGTATCCGGGTCAGCCGCTGGCCGAACGCTTGCGGGTCGGGATGTCGGTAGAGGCGAGCATTGATACCAAAAGCTCAGC
GACGTCGGTGCGTGAGGTGACGCAGCGATGA

Upstream 100 bases:

>100_bases
ATGGCCGGCGTCGCGGTGGCGCTGATCCTGCTGATTCTCTGGCTGCCGACGCGGATCTATCCGCCTCGCGCGCCAACCTG
ACTGGAACAGAAGGTTTTTT

Downstream 100 bases:

>100_bases
AGCGCTTTCAGTCCCTGACAGTGGTCCTGAGTCTGTCGGCATTGGCGGCCTGCACCGTCGGCCCGGACTTCCAGAAACCC
CAAGCCACGCAGATTGCCGA

Product: secretion protein HlyD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 356; Mature: 355

Protein sequence:

>356_residues
MTTQAKQKLAVAVAAALAVGVLVYLAMPGLFGKRTQQNTNDAFVSADFTLVVPRVAGFIKEVLVEDNQQVKAGQLLALID
DRDLRAAAEAADAQTLVARAQLQNAKATLERQSSVIAQAQASVVSAKAEMAFAQQELNRYNHLAGVGAGTVQNAQQARTR
IDQASARLDTATAKLAAERKQVEILTAQRDAAEGSLKHAQAALEIASFELSYTRITAPQDGMIGERAVRVGAYVTPGSKL
LAVVPLKQAYVVANFQETQLTDVQPGQDVVVRVDSLGGEALTGRVESIAPATGVTFAAVKPDNATGNFTKVVQRIPVKVL
LYPGQPLAERLRVGMSVEASIDTKSSATSVREVTQR

Sequences:

>Translated_356_residues
MTTQAKQKLAVAVAAALAVGVLVYLAMPGLFGKRTQQNTNDAFVSADFTLVVPRVAGFIKEVLVEDNQQVKAGQLLALID
DRDLRAAAEAADAQTLVARAQLQNAKATLERQSSVIAQAQASVVSAKAEMAFAQQELNRYNHLAGVGAGTVQNAQQARTR
IDQASARLDTATAKLAAERKQVEILTAQRDAAEGSLKHAQAALEIASFELSYTRITAPQDGMIGERAVRVGAYVTPGSKL
LAVVPLKQAYVVANFQETQLTDVQPGQDVVVRVDSLGGEALTGRVESIAPATGVTFAAVKPDNATGNFTKVVQRIPVKVL
LYPGQPLAERLRVGMSVEASIDTKSSATSVREVTQR
>Mature_355_residues
TTQAKQKLAVAVAAALAVGVLVYLAMPGLFGKRTQQNTNDAFVSADFTLVVPRVAGFIKEVLVEDNQQVKAGQLLALIDD
RDLRAAAEAADAQTLVARAQLQNAKATLERQSSVIAQAQASVVSAKAEMAFAQQELNRYNHLAGVGAGTVQNAQQARTRI
DQASARLDTATAKLAAERKQVEILTAQRDAAEGSLKHAQAALEIASFELSYTRITAPQDGMIGERAVRVGAYVTPGSKLL
AVVPLKQAYVVANFQETQLTDVQPGQDVVVRVDSLGGEALTGRVESIAPATGVTFAAVKPDNATGNFTKVVQRIPVKVLL
YPGQPLAERLRVGMSVEASIDTKSSATSVREVTQR

Specific function: The emr locus confers resistance to substances of high hydrophobicity. EmrA probably participate in a transport system to extrude toxins and drugs from the cell [H]

COG id: COG1566

COG function: function code V; Multidrug resistance efflux pump

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788711, Length=338, Percent_Identity=31.6568047337278, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI1789041, Length=331, Percent_Identity=32.6283987915408, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI1790519, Length=313, Percent_Identity=29.7124600638978, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1789637, Length=313, Percent_Identity=31.629392971246, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI87081951, Length=321, Percent_Identity=27.7258566978193, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1790024, Length=242, Percent_Identity=24.7933884297521, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1790012, Length=245, Percent_Identity=26.1224489795918, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1787013, Length=252, Percent_Identity=30.5555555555556, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005694
- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 37728; Mature: 37597

Theoretical pI: Translated: 9.73; Mature: 9.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTQAKQKLAVAVAAALAVGVLVYLAMPGLFGKRTQQNTNDAFVSADFTLVVPRVAGFIK
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCEEEHHHHHHHHH
EVLVEDNQQVKAGQLLALIDDRDLRAAAEAADAQTLVARAQLQNAKATLERQSSVIAQAQ
HHHHCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ASVVSAKAEMAFAQQELNRYNHLAGVGAGTVQNAQQARTRIDQASARLDTATAKLAAERK
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QVEILTAQRDAAEGSLKHAQAALEIASFELSYTRITAPQDGMIGERAVRVGAYVTPGSKL
HEEEEEECCCCCCCHHHHHHHHHHHHHEEEEEEEECCCCCCCCCHHHHEECEEECCCCCE
LAVVPLKQAYVVANFQETQLTDVQPGQDVVVRVDSLGGEALTGRVESIAPATGVTFAAVK
EEEEECCEEEEEECCCCCCCCCCCCCCCEEEEEECCCCCHHHCHHHHCCCCCCCEEEEEC
PDNATGNFTKVVQRIPVKVLLYPGQPLAERLRVGMSVEASIDTKSSATSVREVTQR
CCCCCCHHHHHHHHCCEEEEEECCCCHHHHHHCCCEEEECCCCCCHHHHHHHHHCC
>Mature Secondary Structure 
TTQAKQKLAVAVAAALAVGVLVYLAMPGLFGKRTQQNTNDAFVSADFTLVVPRVAGFIK
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCEEEHHHHHHHHH
EVLVEDNQQVKAGQLLALIDDRDLRAAAEAADAQTLVARAQLQNAKATLERQSSVIAQAQ
HHHHCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ASVVSAKAEMAFAQQELNRYNHLAGVGAGTVQNAQQARTRIDQASARLDTATAKLAAERK
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QVEILTAQRDAAEGSLKHAQAALEIASFELSYTRITAPQDGMIGERAVRVGAYVTPGSKL
HEEEEEECCCCCCCHHHHHHHHHHHHHEEEEEEEECCCCCCCCCHHHHEECEEECCCCCE
LAVVPLKQAYVVANFQETQLTDVQPGQDVVVRVDSLGGEALTGRVESIAPATGVTFAAVK
EEEEECCEEEEEECCCCCCCCCCCCCCCEEEEEECCCCCHHHCHHHHCCCCCCCEEEEEC
PDNATGNFTKVVQRIPVKVLLYPGQPLAERLRVGMSVEASIDTKSSATSVREVTQR
CCCCCCHHHHHHHHCCEEEEEECCCCHHHHHHCCCEEEECCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]