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The map label for this gene is 77456313

Identifier: 77456313

GI number: 77456313

Start: 90277

End: 92061

Strand: Reverse

Name: 77456313

Synonym: Pfl01_0085

Alternate gene names: NA

Gene position: 92061-90277 (Counterclockwise)

Preceding gene: 77456314

Following gene: 77456312

Centisome position: 1.43

GC content: 61.34

Gene sequence:

>1785_bases
ATGGCAACGGTAATGAAGAAGGTCGACGCAGTGATCGTCGGTTTCGGCTGGACAGGTGCAATCATGGCCAAAGAGCTGAC
CGAGGCCGGCCTCAACGTGCTGGCGCTGGAACGCGGGCCGATGCAGGACACCTACCCTGACGGCAACTATCCCCAGGTGA
TCGACGAACTCACCTACAGCGTGCGGAAAAAACTCTTCCAGGACATTTCCAAAGAGACTGTCACCATCCGTCACAGCGTC
AACGACATCGCCCTGCCAAACCGCCAGTTGGGCGCGTTCCTGCCGGGCAATGGCGTGGGCGGCGCCGGCTTGCACTGGTC
GGGCGTGCACTTTCGGGTCGACCCGATCGAGTTGCGCATGCGCAGCCACTACGAAGAGCGCTACGGCAAAAGCTTCATCC
CCAAGGACATGACCATCCAGGACTTCGGCGTCAGCTATGAAGAGCTGGAGCCGTTCTTCGATTTCGCCGAAAAAGTCTTC
GGCACCTCCGGCCAGGCCTGGACGGTAAAAGGCCAACTGGTCGGCCAGGGCAAGGGCGGTAACCCGTACGCACCGGATCG
CTCGAATCCGTTCCCGCTGCAAGCGCAGAAGAACACGGTTTCCGCACAGCTGTTCGGCAAAGCGGCTACCGAAGTCGGTT
ACAAACCCTACAACCTGCCATCCGCGAATACGTCAGGGCCGTACACCAACCCCTACGGCGCGCAGATGGGCCCGTGCAAC
TTCTGCGGTTTCTGCAGTGGCTACGTTTGCTACATGTATTCCAAGGCTTCGCCAAACGTGAACATTCTGCCGGCGCTGAA
GCCACTGCCGAATTTCGAATTGCGGCCTAACGCTCATGTGCTGCGGGTCAACCTCGACAGCACGAAAACCAAAGCCACCG
GCGTCACCTACATCGACGGCCAGGGCCGGGAGATCGAGCAACCGGCGGATCTGGTGATCCTCGGCGCGTTCCAGTTGCAC
AACGTGCGCCTGATGCTGCTCTCCGGCATCGGCAAACCCTACGACCCGGTCAGCGGTGAAGGTGTGGTCGGGCGCAACTT
TGCCTACCAGAACATGGCCACCATCAAGGCGTTCTTCGACAAGAACACCCACACCAACAACTTCATCGGTGCCGGCGGCA
ACGGTGTGGCGGTAGATGACTTCAACGCCGACAACTTCGATCACGGGCCGCATGGCTTCGTCGGTGGCTCGCCGATGTGG
GTCAACCAGGCCGGCAGCCGACCGATCGCCGGCACCTCCAACCCACCGGGCACCCCGGCCTGGGGCAGTGCGTGGAAACG
CGCCACTGCCGATTACTACACCCACCAGGTGTCGATGGACGCCCACGGCGCGCATCAGTCCTACCGTGGCAACTACCTCG
ATCTGGACCCGGTGTACCGCGATGCCTACGGCATGCCGCTGCTGCGGATGACGTTCGACTGGCAGGAAAACGACATCAAG
ATGAACCGCTTCATGGTCGAGAAAATGGGCAAGATCGCCGAGGCCATGGGCCCGAAAGCCATCGCCGTGATCGGCAAGAA
AGTCGGCGACCACTTCAACACCGCGTCGTACCAGACCACCCACCTCAACGGTGGCGCGATCATGGGCACCGATCCGAAGA
CCAGCGCGCTCAACCGCTACCTGCAGAGTTGGGACGTGCACAACGTATTCGTCCCGGGCGCCTCGGCATTCCCGCAAGGC
CTGGGTTACAACCCGACCGGCCTGGTTGCCGCGCTGACCTACTGGTCGGCGAAAGCGATCCGCGAGCAATACCTGAAAAA
CCCCGGCCCGCTGGTTCAGGCTTAA

Upstream 100 bases:

>100_bases
TCGGCTTCCCCGGCGCCCGCGCCGATTTCATGGATTGGGTGGAACGCAACGAGCAATACCCCTTCCCGGCAGTTTCGATT
CGCGGCAAGAGGGCGTGAGC

Downstream 100 bases:

>100_bases
GGAGCGATGACCATGAAAACTCTCGTTATCGCGACCCTGGCGCTGCTCGGCAGCGCTGCCGTTCATGGCGCCGAAGTTGA
TCAATCCCTGATCAAAAAAG

Product: glucose-methanol-choline oxidoreductase

Products: NA

Alternate protein names: GA 2-DH dehydrogenase subunit; GADH dehydrogenase subunit [H]

Number of amino acids: Translated: 594; Mature: 593

Protein sequence:

>594_residues
MATVMKKVDAVIVGFGWTGAIMAKELTEAGLNVLALERGPMQDTYPDGNYPQVIDELTYSVRKKLFQDISKETVTIRHSV
NDIALPNRQLGAFLPGNGVGGAGLHWSGVHFRVDPIELRMRSHYEERYGKSFIPKDMTIQDFGVSYEELEPFFDFAEKVF
GTSGQAWTVKGQLVGQGKGGNPYAPDRSNPFPLQAQKNTVSAQLFGKAATEVGYKPYNLPSANTSGPYTNPYGAQMGPCN
FCGFCSGYVCYMYSKASPNVNILPALKPLPNFELRPNAHVLRVNLDSTKTKATGVTYIDGQGREIEQPADLVILGAFQLH
NVRLMLLSGIGKPYDPVSGEGVVGRNFAYQNMATIKAFFDKNTHTNNFIGAGGNGVAVDDFNADNFDHGPHGFVGGSPMW
VNQAGSRPIAGTSNPPGTPAWGSAWKRATADYYTHQVSMDAHGAHQSYRGNYLDLDPVYRDAYGMPLLRMTFDWQENDIK
MNRFMVEKMGKIAEAMGPKAIAVIGKKVGDHFNTASYQTTHLNGGAIMGTDPKTSALNRYLQSWDVHNVFVPGASAFPQG
LGYNPTGLVAALTYWSAKAIREQYLKNPGPLVQA

Sequences:

>Translated_594_residues
MATVMKKVDAVIVGFGWTGAIMAKELTEAGLNVLALERGPMQDTYPDGNYPQVIDELTYSVRKKLFQDISKETVTIRHSV
NDIALPNRQLGAFLPGNGVGGAGLHWSGVHFRVDPIELRMRSHYEERYGKSFIPKDMTIQDFGVSYEELEPFFDFAEKVF
GTSGQAWTVKGQLVGQGKGGNPYAPDRSNPFPLQAQKNTVSAQLFGKAATEVGYKPYNLPSANTSGPYTNPYGAQMGPCN
FCGFCSGYVCYMYSKASPNVNILPALKPLPNFELRPNAHVLRVNLDSTKTKATGVTYIDGQGREIEQPADLVILGAFQLH
NVRLMLLSGIGKPYDPVSGEGVVGRNFAYQNMATIKAFFDKNTHTNNFIGAGGNGVAVDDFNADNFDHGPHGFVGGSPMW
VNQAGSRPIAGTSNPPGTPAWGSAWKRATADYYTHQVSMDAHGAHQSYRGNYLDLDPVYRDAYGMPLLRMTFDWQENDIK
MNRFMVEKMGKIAEAMGPKAIAVIGKKVGDHFNTASYQTTHLNGGAIMGTDPKTSALNRYLQSWDVHNVFVPGASAFPQG
LGYNPTGLVAALTYWSAKAIREQYLKNPGPLVQA
>Mature_593_residues
ATVMKKVDAVIVGFGWTGAIMAKELTEAGLNVLALERGPMQDTYPDGNYPQVIDELTYSVRKKLFQDISKETVTIRHSVN
DIALPNRQLGAFLPGNGVGGAGLHWSGVHFRVDPIELRMRSHYEERYGKSFIPKDMTIQDFGVSYEELEPFFDFAEKVFG
TSGQAWTVKGQLVGQGKGGNPYAPDRSNPFPLQAQKNTVSAQLFGKAATEVGYKPYNLPSANTSGPYTNPYGAQMGPCNF
CGFCSGYVCYMYSKASPNVNILPALKPLPNFELRPNAHVLRVNLDSTKTKATGVTYIDGQGREIEQPADLVILGAFQLHN
VRLMLLSGIGKPYDPVSGEGVVGRNFAYQNMATIKAFFDKNTHTNNFIGAGGNGVAVDDFNADNFDHGPHGFVGGSPMWV
NQAGSRPIAGTSNPPGTPAWGSAWKRATADYYTHQVSMDAHGAHQSYRGNYLDLDPVYRDAYGMPLLRMTFDWQENDIKM
NRFMVEKMGKIAEAMGPKAIAVIGKKVGDHFNTASYQTTHLNGGAIMGTDPKTSALNRYLQSWDVHNVFVPGASAFPQGL
GYNPTGLVAALTYWSAKAIREQYLKNPGPLVQA

Specific function: Part of the heterotrimer that catalyzes the conversion of D-gluconate to 2-dehydro-D-gluconate. This subunit functions as the dehydrogenase [H]

COG id: COG2303

COG function: function code E; Choline dehydrogenase and related flavoproteins

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Periplasmic side (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GMC oxidoreductase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000172
- InterPro:   IPR007867 [H]

Pfam domain/function: PF05199 GMC_oxred_C; PF00732 GMC_oxred_N [H]

EC number: =1.1.99.3 [H]

Molecular weight: Translated: 64860; Mature: 64729

Theoretical pI: Translated: 8.26; Mature: 8.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATVMKKVDAVIVGFGWTGAIMAKELTEAGLNVLALERGPMQDTYPDGNYPQVIDELTYS
CCHHHHCCCEEEEECCCHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHH
VRKKLFQDISKETVTIRHSVNDIALPNRQLGAFLPGNGVGGAGLHWSGVHFRVDPIELRM
HHHHHHHHHCCCEEEEEECCCCCCCCCCCCCEECCCCCCCCCCCEECCEEEEECHHHHHH
RSHYEERYGKSFIPKDMTIQDFGVSYEELEPFFDFAEKVFGTSGQAWTVKGQLVGQGKGG
HHHHHHHHCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCC
NPYAPDRSNPFPLQAQKNTVSAQLFGKAATEVGYKPYNLPSANTSGPYTNPYGAQMGPCN
CCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
FCGFCSGYVCYMYSKASPNVNILPALKPLPNFELRPNAHVLRVNLDSTKTKATGVTYIDG
HHHHHCCEEEEEEECCCCCCEEEECCCCCCCCCCCCCCEEEEEECCCCCCCCCCEEEECC
QGREIEQPADLVILGAFQLHNVRLMLLSGIGKPYDPVSGEGVVGRNFAYQNMATIKAFFD
CCCCCCCCCCEEEEEEEECCCEEEEEECCCCCCCCCCCCCCEECCCCCHHHHHHHHHHHC
KNTHTNNFIGAGGNGVAVDDFNADNFDHGPHGFVGGSPMWVNQAGSRPIAGTSNPPGTPA
CCCCCCCEECCCCCEEEEECCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCC
WGSAWKRATADYYTHQVSMDAHGAHQSYRGNYLDLDPVYRDAYGMPLLRMTFDWQENDIK
HHHHHHHHHHHHHEEEEECCCCCCCHHCCCCCEECCHHHHHHCCCCEEEEEECCCCCCCH
MNRFMVEKMGKIAEAMGPKAIAVIGKKVGDHFNTASYQTTHLNGGAIMGTDPKTSALNRY
HHHHHHHHHHHHHHHCCCCEEEEHHHHHCCCCCCCCEEEEEECCCEEECCCCCHHHHHHH
LQSWDVHNVFVPGASAFPQGLGYNPTGLVAALTYWSAKAIREQYLKNPGPLVQA
HHCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
ATVMKKVDAVIVGFGWTGAIMAKELTEAGLNVLALERGPMQDTYPDGNYPQVIDELTYS
CHHHHCCCEEEEECCCHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHH
VRKKLFQDISKETVTIRHSVNDIALPNRQLGAFLPGNGVGGAGLHWSGVHFRVDPIELRM
HHHHHHHHHCCCEEEEEECCCCCCCCCCCCCEECCCCCCCCCCCEECCEEEEECHHHHHH
RSHYEERYGKSFIPKDMTIQDFGVSYEELEPFFDFAEKVFGTSGQAWTVKGQLVGQGKGG
HHHHHHHHCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCC
NPYAPDRSNPFPLQAQKNTVSAQLFGKAATEVGYKPYNLPSANTSGPYTNPYGAQMGPCN
CCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
FCGFCSGYVCYMYSKASPNVNILPALKPLPNFELRPNAHVLRVNLDSTKTKATGVTYIDG
HHHHHCCEEEEEEECCCCCCEEEECCCCCCCCCCCCCCEEEEEECCCCCCCCCCEEEECC
QGREIEQPADLVILGAFQLHNVRLMLLSGIGKPYDPVSGEGVVGRNFAYQNMATIKAFFD
CCCCCCCCCCEEEEEEEECCCEEEEEECCCCCCCCCCCCCCEECCCCCHHHHHHHHHHHC
KNTHTNNFIGAGGNGVAVDDFNADNFDHGPHGFVGGSPMWVNQAGSRPIAGTSNPPGTPA
CCCCCCCEECCCCCEEEEECCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCC
WGSAWKRATADYYTHQVSMDAHGAHQSYRGNYLDLDPVYRDAYGMPLLRMTFDWQENDIK
HHHHHHHHHHHHHEEEEECCCCCCCHHCCCCCEECCHHHHHHCCCCEEEEEECCCCCCCH
MNRFMVEKMGKIAEAMGPKAIAVIGKKVGDHFNTASYQTTHLNGGAIMGTDPKTSALNRY
HHHHHHHHHHHHHHHCCCCEEEEHHHHHCCCCCCCCEEEEEECCCEEECCCCCHHHHHHH
LQSWDVHNVFVPGASAFPQGLGYNPTGLVAALTYWSAKAIREQYLKNPGPLVQA
HHCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9352901 [H]