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The map label for this gene is gyrB [H]

Identifier: 77456232

GI number: 77456232

Start: 4355

End: 6772

Strand: Direct

Name: gyrB [H]

Synonym: Pfl01_0004

Alternate gene names: 77456232

Gene position: 4355-6772 (Clockwise)

Preceding gene: 77456231

Following gene: 77456234

Centisome position: 0.07

GC content: 58.4

Gene sequence:

>2418_bases
TTGAGCGAAGAAAATACGTACGACTCATCGAGCATTAAAGTGCTGAAAGGCCTGGATGCCGTGCGCAAACGTCCCGGTAT
GTACATTGGTGACACCGACGATGGCAGCGGTCTGCACCACATGGTGTTCGAGGTGGTCGACAACTCGATCGACGAAGCCC
TCGCCGGCCACTGCGACGACATCAGCATCATCATCCACCCGGATGAGTCCATCACCGTTAAAGACAACGGCCGTGGCATC
CCGGTAGACGTGCACAAAGAGGAAGGCGTTTCCGCCGCCGAGGTCATCATGACCGTCCTCCACGCCGGCGGTAAGTTTGA
CGACAACTCCTACAAGGTATCCGGTGGTCTGCACGGTGTAGGTGTTTCGGTCGTGAACGCGCTGTCTGAAGAACTGGTCC
TGACCGTGCGCCGCAGCGGCAAGATCTGGGAACAGACCTACGTCCACGGCGTGCCTCAGGCACCGATGGCGATCGTTGGC
GACAGCGAAACCACTGGCACCCAGATTCACTTCAAGGCTTCCAGCGAAACCTTCAAGAACATTCACTTCAGCTGGGATAT
CCTGGCCAAGCGCATTCGTGAACTGTCTTTCCTCAACTCCGGTGTAGGCATCGTCCTCAAGGACGAGCGCAGCGGCAAGG
AAGAACTGTTCAAGTACGAAGGCGGCCTGCGTGCGTTCGTTGAATACCTGAACACCAACAAGACTGCGGTCAACCAGGTG
TTCCACTTCAACATCCAGCGTGAAGACGGCATCGGCGTGGAAATCGCCCTGCAGTGGAACGACAGCTTCAACGAGAACCT
GTTGTGCTTCACCAACAACATTCCGCAGCGCGACGGTGGCACTCACCTGGTGGGCTTCCGCTCGGCACTGACGCGTAACC
TGAACAACTACATCGAGCAGGAAGGCCTGGCGAAGAAGCACAAAGTCGCCACCACCGGTGACGATGCCCGTGAAGGCCTG
ACCGCGATCATCTCGGTGAAGGTGCCGGATCCGAAGTTCAGCTCCCAGACCAAAGACAAGCTGGTTTCTTCCGAAGTGAA
GACCGCGGTCGAACAGGAAATGGGCAAGTACTTCTCCGACTTCCTGCTGGAAAACCCGAACGAAGCCAAACTGGTCGTCG
GCAAGATGATCGACGCTGCCCGTGCCCGTGAAGCGGCGCGCAAGGCCCGTGAGATGACCCGCCGCAAAGGCGCGCTGGAC
ATCGCCGGCCTGCCGGGCAAGCTCGCTGACTGCCAGGAAAAAGACCCGGCGCTGTCCGAACTGTACCTCGTGGAAGGTGA
CTCCGCGGGCGGCTCTGCCAAGCAGGGACGTAACCGCAAGACCCAGGCCATCCTGCCGCTGAAGGGCAAGATCCTCAACG
TCGAGAAAGCCCGTTTCGACAAGATGATCTCGTCCCAGGAAGTGGGCACCCTGATCACCGCGCTGGGCTGCGGTATCGGT
CGCGAAGAGTACAACATCGACAAGCTGCGCTATCACAACATCATCATCATGACCGATGCTGACGTCGACGGTTCGCACAT
CCGTACCCTGCTGCTGACCTTCTTCTTCCGTCAGTTGCCTGAGCTGATCGAGCGTGGCTACATCTACATCGCCCAGCCGC
CGCTGTACAAGGTCAAGAAAGGCAAGCAAGAGCAATACATCAAAGACGACGACGCCATGGAAGAGTACATGACGCAGTCG
GCCCTGGAAGATGCGAGCCTGCACCTGAACGAAGACGCCCCGGGCATTTCCGGCGAAGCGCTGGAACGTCTGGTGAACGA
CTTCCGCATGGTGATGAAGACCCTCAAGCGTCTGTCGCGCCTGTACCCGCAGGAGCTGACCGAGCACTTCATCTACCTGC
CGGCCGTCAGCCTGGAAATGCTCGGCGACCACGCGAAGATGCAGGACTGGCTGGCCCAGTACGAAGTCCGTCTGCGCACC
GTCGAGAAGTCGGGCCTGGTCTACAAGGCCAGCCTGCGTGAAGATCGTGAACGTGGCGTTTGGCTGCCAGAGGTCGAACT
GATCTCCCACGGTCTGTCGAACTATGTCACCTTTAACCGCGACTTCTTCGGCAGCAACGACTACAAGACCGTCGTCACCC
TCGGCGCTCAACTGAGCACCCTTCTTGATGAAGGCGCATACATCCAGCGTGGCGAGCGTAAAAAAGCGGTCACTGAGTTC
AAGGAAGCCCTGGACTGGCTGATGGCCGAAAGCACCAAGCGCCACACCATTCAGCGATACAAAGGTCTGGGCGAAATGAA
CCCGGATCAGCTGTGGGAAACCACCATGGACCCAAGCGTGCGCCGCATGCTGAAAGTCACCATCGAAGACGCCATTGGCG
CAGACCAGATCTTCAACACCCTGATGGGTGATGCGGTCGAACCTCGCCGTGACTTCATCGAGAGCAACGCTCTGGCGGTG
TCCAACCTGGATTTCTGA

Upstream 100 bases:

>100_bases
AATTACTGAGGGAAGGCTGGCAGACGGAAACGCCAGTCGCTCTGTTCCACGTGGAACAGGGCCGTATCACCCAGACCCAC
GACCATCGGGAGTGAAGGCA

Downstream 100 bases:

>100_bases
TCCTGGTCAGCACCAACGAAAAGGCCAACGCACCAGCGTTGGCCTTTTTTATTGCCTGCGATTCACTCGCCTGCCGCCAC
ACTCTCCAAACGATACCCAT

Product: DNA gyrase subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 805; Mature: 804

Protein sequence:

>805_residues
MSEENTYDSSSIKVLKGLDAVRKRPGMYIGDTDDGSGLHHMVFEVVDNSIDEALAGHCDDISIIIHPDESITVKDNGRGI
PVDVHKEEGVSAAEVIMTVLHAGGKFDDNSYKVSGGLHGVGVSVVNALSEELVLTVRRSGKIWEQTYVHGVPQAPMAIVG
DSETTGTQIHFKASSETFKNIHFSWDILAKRIRELSFLNSGVGIVLKDERSGKEELFKYEGGLRAFVEYLNTNKTAVNQV
FHFNIQREDGIGVEIALQWNDSFNENLLCFTNNIPQRDGGTHLVGFRSALTRNLNNYIEQEGLAKKHKVATTGDDAREGL
TAIISVKVPDPKFSSQTKDKLVSSEVKTAVEQEMGKYFSDFLLENPNEAKLVVGKMIDAARAREAARKAREMTRRKGALD
IAGLPGKLADCQEKDPALSELYLVEGDSAGGSAKQGRNRKTQAILPLKGKILNVEKARFDKMISSQEVGTLITALGCGIG
REEYNIDKLRYHNIIIMTDADVDGSHIRTLLLTFFFRQLPELIERGYIYIAQPPLYKVKKGKQEQYIKDDDAMEEYMTQS
ALEDASLHLNEDAPGISGEALERLVNDFRMVMKTLKRLSRLYPQELTEHFIYLPAVSLEMLGDHAKMQDWLAQYEVRLRT
VEKSGLVYKASLREDRERGVWLPEVELISHGLSNYVTFNRDFFGSNDYKTVVTLGAQLSTLLDEGAYIQRGERKKAVTEF
KEALDWLMAESTKRHTIQRYKGLGEMNPDQLWETTMDPSVRRMLKVTIEDAIGADQIFNTLMGDAVEPRRDFIESNALAV
SNLDF

Sequences:

>Translated_805_residues
MSEENTYDSSSIKVLKGLDAVRKRPGMYIGDTDDGSGLHHMVFEVVDNSIDEALAGHCDDISIIIHPDESITVKDNGRGI
PVDVHKEEGVSAAEVIMTVLHAGGKFDDNSYKVSGGLHGVGVSVVNALSEELVLTVRRSGKIWEQTYVHGVPQAPMAIVG
DSETTGTQIHFKASSETFKNIHFSWDILAKRIRELSFLNSGVGIVLKDERSGKEELFKYEGGLRAFVEYLNTNKTAVNQV
FHFNIQREDGIGVEIALQWNDSFNENLLCFTNNIPQRDGGTHLVGFRSALTRNLNNYIEQEGLAKKHKVATTGDDAREGL
TAIISVKVPDPKFSSQTKDKLVSSEVKTAVEQEMGKYFSDFLLENPNEAKLVVGKMIDAARAREAARKAREMTRRKGALD
IAGLPGKLADCQEKDPALSELYLVEGDSAGGSAKQGRNRKTQAILPLKGKILNVEKARFDKMISSQEVGTLITALGCGIG
REEYNIDKLRYHNIIIMTDADVDGSHIRTLLLTFFFRQLPELIERGYIYIAQPPLYKVKKGKQEQYIKDDDAMEEYMTQS
ALEDASLHLNEDAPGISGEALERLVNDFRMVMKTLKRLSRLYPQELTEHFIYLPAVSLEMLGDHAKMQDWLAQYEVRLRT
VEKSGLVYKASLREDRERGVWLPEVELISHGLSNYVTFNRDFFGSNDYKTVVTLGAQLSTLLDEGAYIQRGERKKAVTEF
KEALDWLMAESTKRHTIQRYKGLGEMNPDQLWETTMDPSVRRMLKVTIEDAIGADQIFNTLMGDAVEPRRDFIESNALAV
SNLDF
>Mature_804_residues
SEENTYDSSSIKVLKGLDAVRKRPGMYIGDTDDGSGLHHMVFEVVDNSIDEALAGHCDDISIIIHPDESITVKDNGRGIP
VDVHKEEGVSAAEVIMTVLHAGGKFDDNSYKVSGGLHGVGVSVVNALSEELVLTVRRSGKIWEQTYVHGVPQAPMAIVGD
SETTGTQIHFKASSETFKNIHFSWDILAKRIRELSFLNSGVGIVLKDERSGKEELFKYEGGLRAFVEYLNTNKTAVNQVF
HFNIQREDGIGVEIALQWNDSFNENLLCFTNNIPQRDGGTHLVGFRSALTRNLNNYIEQEGLAKKHKVATTGDDAREGLT
AIISVKVPDPKFSSQTKDKLVSSEVKTAVEQEMGKYFSDFLLENPNEAKLVVGKMIDAARAREAARKAREMTRRKGALDI
AGLPGKLADCQEKDPALSELYLVEGDSAGGSAKQGRNRKTQAILPLKGKILNVEKARFDKMISSQEVGTLITALGCGIGR
EEYNIDKLRYHNIIIMTDADVDGSHIRTLLLTFFFRQLPELIERGYIYIAQPPLYKVKKGKQEQYIKDDDAMEEYMTQSA
LEDASLHLNEDAPGISGEALERLVNDFRMVMKTLKRLSRLYPQELTEHFIYLPAVSLEMLGDHAKMQDWLAQYEVRLRTV
EKSGLVYKASLREDRERGVWLPEVELISHGLSNYVTFNRDFFGSNDYKTVVTLGAQLSTLLDEGAYIQRGERKKAVTEFK
EALDWLMAESTKRHTIQRYKGLGEMNPDQLWETTMDPSVRRMLKVTIEDAIGADQIFNTLMGDAVEPRRDFIESNALAVS
NLDF

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type II topoisomerase family [H]

Homologues:

Organism=Homo sapiens, GI19913406, Length=572, Percent_Identity=25.5244755244755, Blast_Score=132, Evalue=2e-30,
Organism=Homo sapiens, GI19913408, Length=550, Percent_Identity=25.4545454545455, Blast_Score=125, Evalue=2e-28,
Organism=Escherichia coli, GI48994957, Length=803, Percent_Identity=67.4968866749689, Blast_Score=1121, Evalue=0.0,
Organism=Escherichia coli, GI1789408, Length=558, Percent_Identity=39.7849462365591, Blast_Score=382, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI17535065, Length=538, Percent_Identity=26.2081784386617, Blast_Score=135, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI212645845, Length=533, Percent_Identity=25.328330206379, Blast_Score=125, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI212645657, Length=153, Percent_Identity=33.3333333333333, Blast_Score=74, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6324241, Length=596, Percent_Identity=25.3355704697987, Blast_Score=126, Evalue=1e-29,
Organism=Drosophila melanogaster, GI17136538, Length=557, Percent_Identity=26.032315978456, Blast_Score=142, Evalue=9e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 90134; Mature: 90003

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEENTYDSSSIKVLKGLDAVRKRPGMYIGDTDDGSGLHHMVFEVVDNSIDEALAGHCDD
CCCCCCCCCHHHHHHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCC
ISIIIHPDESITVKDNGRGIPVDVHKEEGVSAAEVIMTVLHAGGKFDDNSYKVSGGLHGV
EEEEEECCCEEEEECCCCCCCEEECCCCCCCHHHHHHHHHHCCCCCCCCCEEECCCCCHH
GVSVVNALSEELVLTVRRSGKIWEQTYVHGVPQAPMAIVGDSETTGTQIHFKASSETFKN
HHHHHHHHHHHHEEHHHCCCCHHHHHHHCCCCCCCEEEECCCCCCCCEEEEEECCHHHHH
IHFSWDILAKRIRELSFLNSGVGIVLKDERSGKEELFKYEGGLRAFVEYLNTNKTAVNQV
CCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHCCHHHHHHHHHCCCHHHHHHH
FHFNIQREDGIGVEIALQWNDSFNENLLCFTNNIPQRDGGTHLVGFRSALTRNLNNYIEQ
HEEEEEECCCCCEEEEEEECCCCCCCEEEEECCCCCCCCCCEEEHHHHHHHHHHHHHHHH
EGLAKKHKVATTGDDAREGLTAIISVKVPDPKFSSQTKDKLVSSEVKTAVEQEMGKYFSD
HCCHHHCCCCCCCCHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
FLLENPNEAKLVVGKMIDAARAREAARKAREMTRRKGALDIAGLPGKLADCQEKDPALSE
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCE
LYLVEGDSAGGSAKQGRNRKTQAILPLKGKILNVEKARFDKMISSQEVGTLITALGCGIG
EEEEECCCCCCCCCCCCCCCCEEEECCCCCEECHHHHHHHHHHHHHHHHHHHHHHHCCCC
REEYNIDKLRYHNIIIMTDADVDGSHIRTLLLTFFFRQLPELIERGYIYIAQPPLYKVKK
CCCCCCCEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCHHHHC
GKQEQYIKDDDAMEEYMTQSALEDASLHLNEDAPGISGEALERLVNDFRMVMKTLKRLSR
CCCHHCCCCHHHHHHHHHHHHHHHCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LYPQELTEHFIYLPAVSLEMLGDHAKMQDWLAQYEVRLRTVEKSGLVYKASLREDRERGV
HCHHHHHHHEEECCCHHHHHHCCHHHHHHHHHHHHHHHEEECCCCCEEEEHHHHHHHCCC
WLPEVELISHGLSNYVTFNRDFFGSNDYKTVVTLGAQLSTLLDEGAYIQRGERKKAVTEF
CCCHHHHHHCCHHHCEEECCCCCCCCCCEEEEEHHHHHHHHHHCCCHHHCCCHHHHHHHH
KEALDWLMAESTKRHTIQRYKGLGEMNPDQLWETTMDPSVRRMLKVTIEDAIGADQIFNT
HHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHH
LMGDAVEPRRDFIESNALAVSNLDF
HHCCCCCHHHHHHHCCCEEECCCCC
>Mature Secondary Structure 
SEENTYDSSSIKVLKGLDAVRKRPGMYIGDTDDGSGLHHMVFEVVDNSIDEALAGHCDD
CCCCCCCCHHHHHHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCC
ISIIIHPDESITVKDNGRGIPVDVHKEEGVSAAEVIMTVLHAGGKFDDNSYKVSGGLHGV
EEEEEECCCEEEEECCCCCCCEEECCCCCCCHHHHHHHHHHCCCCCCCCCEEECCCCCHH
GVSVVNALSEELVLTVRRSGKIWEQTYVHGVPQAPMAIVGDSETTGTQIHFKASSETFKN
HHHHHHHHHHHHEEHHHCCCCHHHHHHHCCCCCCCEEEECCCCCCCCEEEEEECCHHHHH
IHFSWDILAKRIRELSFLNSGVGIVLKDERSGKEELFKYEGGLRAFVEYLNTNKTAVNQV
CCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHCCHHHHHHHHHCCCHHHHHHH
FHFNIQREDGIGVEIALQWNDSFNENLLCFTNNIPQRDGGTHLVGFRSALTRNLNNYIEQ
HEEEEEECCCCCEEEEEEECCCCCCCEEEEECCCCCCCCCCEEEHHHHHHHHHHHHHHHH
EGLAKKHKVATTGDDAREGLTAIISVKVPDPKFSSQTKDKLVSSEVKTAVEQEMGKYFSD
HCCHHHCCCCCCCCHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
FLLENPNEAKLVVGKMIDAARAREAARKAREMTRRKGALDIAGLPGKLADCQEKDPALSE
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCE
LYLVEGDSAGGSAKQGRNRKTQAILPLKGKILNVEKARFDKMISSQEVGTLITALGCGIG
EEEEECCCCCCCCCCCCCCCCEEEECCCCCEECHHHHHHHHHHHHHHHHHHHHHHHCCCC
REEYNIDKLRYHNIIIMTDADVDGSHIRTLLLTFFFRQLPELIERGYIYIAQPPLYKVKK
CCCCCCCEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCHHHHC
GKQEQYIKDDDAMEEYMTQSALEDASLHLNEDAPGISGEALERLVNDFRMVMKTLKRLSR
CCCHHCCCCHHHHHHHHHHHHHHHCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LYPQELTEHFIYLPAVSLEMLGDHAKMQDWLAQYEVRLRTVEKSGLVYKASLREDRERGV
HCHHHHHHHEEECCCHHHHHHCCHHHHHHHHHHHHHHHEEECCCCCEEEEHHHHHHHCCC
WLPEVELISHGLSNYVTFNRDFFGSNDYKTVVTLGAQLSTLLDEGAYIQRGERKKAVTEF
CCCHHHHHHCCHHHCEEECCCCCCCCCCEEEEEHHHHHHHHHHCCCHHHCCCHHHHHHHH
KEALDWLMAESTKRHTIQRYKGLGEMNPDQLWETTMDPSVRRMLKVTIEDAIGADQIFNT
HHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHH
LMGDAVEPRRDFIESNALAVSNLDF
HHCCCCCHHHHHHHCCCEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2170947; 7793912; 2540413 [H]