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The map label for this gene is dnaN [H]

Identifier: 77456230

GI number: 77456230

Start: 2128

End: 3231

Strand: Direct

Name: dnaN [H]

Synonym: Pfl01_0002

Alternate gene names: 77456230

Gene position: 2128-3231 (Clockwise)

Preceding gene: 77456229

Following gene: 77456231

Centisome position: 0.03

GC content: 60.33

Gene sequence:

>1104_bases
ATGCATTTCACCATTCAACGCGAAGCCCTGTTGAAACCCCTGCAACTGGTCGCAGGCGTCGTCGAGCGCCGACAGACCTT
GCCGGTACTGTCCAACGTGCTGCTGGTTGTCGATGGCCAACAACTGTCGCTGACCGGTACCGACCTGGAAGTCGAGCTGG
TCGGTCGCGTGCAACTCGAGGAGCCGGCTGAAACAGGTTCCATCACCGTGCCGGCGCGCAAGCTGATGGACATCTGCAAG
AGCCTGCCGAACGATGCGCTGATCGACATCAAGGTCGACGAGCAGAAGCTGGTGGTGAAAGCCGGCCGTAGCCGCTTCAC
CCTCTCGACGCTGCCGGCCAACGACTTCCCGACTGTGGAAGAAGGCCCGGGGTCGCTGACTACCAGTCTGGAACAGAGCA
AGCTGCGTCGCCTGATCGAACGCACCAGTTTCGCCATGGCCCAGCAGGACGTGCGTTACTACCTCAACGGCATGTTGCTG
GAAGTGTCGACCGGTGTGATCCGTGCCGTGGCTACCGACGGTCACCGTCTGGCCATGTGCTCGATGCAGGCCGACATCGG
CCAGCAGGATCGCCACCAAGTGATCGTGCCGCGCAAAGGTATTCTTGAACTGGCACGTCTGCTCACCGAGCCGGACGGCA
ACGTCAGCATCGTCCTGGGCCAGCACCACATCCGTGCGACCACCGGCGAATTCACCTTCACCTCGAAACTGGTCGACGGC
AAGTTCCCGGACTACGAGCGCGTTCTGCCAAAAGGTGGCGACAAGCTGGTACTGGGCGATCGTCAGGCGCTGCGTGAAGC
GTTCAGCCGTACCGCGATTCTGTCCAACGAGAAGTACCGCGGTATCCGTCTGCAACTGGCCAGCGGTCAGTTGAAGATCC
AGGCCAACAACCCGGAGCAGGAAGAAGCGGAAGAAGAAGTAGGCGTGGAATACAACGGCGGCTCCCTGGAAATCGGCTTC
AACGTCAGCTATCTGCTGGACGTGCTGGGCGTGATGACCACCGAGCAGGTTCGTCTGATCCTGTCCGACTCCAACAGCAG
TGCGCTGGTGCAAGAGTCCGACAATGACGATTCGGCTTACGTTGTCATGCCGATGCGTCTGTAA

Upstream 100 bases:

>100_bases
GGAATCCGACGCGGACATCCGCGAGGACTACAAGAACCTGCTGCGTACACTGACCACTTGATGAACACCAGCGCAGCTTA
TTAAGGCAAGGGACTAGACC

Downstream 100 bases:

>100_bases
TCAGCTGATTCTGGATGTCGCTAAGTCGCGTCTCGGTCACCGCGGTGCGCAATCTGCACCCGGTGACCTTCTCCCCCTCC
CCCCGAATCAACATTCTTTA

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 367; Mature: 367

Protein sequence:

>367_residues
MHFTIQREALLKPLQLVAGVVERRQTLPVLSNVLLVVDGQQLSLTGTDLEVELVGRVQLEEPAETGSITVPARKLMDICK
SLPNDALIDIKVDEQKLVVKAGRSRFTLSTLPANDFPTVEEGPGSLTTSLEQSKLRRLIERTSFAMAQQDVRYYLNGMLL
EVSTGVIRAVATDGHRLAMCSMQADIGQQDRHQVIVPRKGILELARLLTEPDGNVSIVLGQHHIRATTGEFTFTSKLVDG
KFPDYERVLPKGGDKLVLGDRQALREAFSRTAILSNEKYRGIRLQLASGQLKIQANNPEQEEAEEEVGVEYNGGSLEIGF
NVSYLLDVLGVMTTEQVRLILSDSNSSALVQESDNDDSAYVVMPMRL

Sequences:

>Translated_367_residues
MHFTIQREALLKPLQLVAGVVERRQTLPVLSNVLLVVDGQQLSLTGTDLEVELVGRVQLEEPAETGSITVPARKLMDICK
SLPNDALIDIKVDEQKLVVKAGRSRFTLSTLPANDFPTVEEGPGSLTTSLEQSKLRRLIERTSFAMAQQDVRYYLNGMLL
EVSTGVIRAVATDGHRLAMCSMQADIGQQDRHQVIVPRKGILELARLLTEPDGNVSIVLGQHHIRATTGEFTFTSKLVDG
KFPDYERVLPKGGDKLVLGDRQALREAFSRTAILSNEKYRGIRLQLASGQLKIQANNPEQEEAEEEVGVEYNGGSLEIGF
NVSYLLDVLGVMTTEQVRLILSDSNSSALVQESDNDDSAYVVMPMRL
>Mature_367_residues
MHFTIQREALLKPLQLVAGVVERRQTLPVLSNVLLVVDGQQLSLTGTDLEVELVGRVQLEEPAETGSITVPARKLMDICK
SLPNDALIDIKVDEQKLVVKAGRSRFTLSTLPANDFPTVEEGPGSLTTSLEQSKLRRLIERTSFAMAQQDVRYYLNGMLL
EVSTGVIRAVATDGHRLAMCSMQADIGQQDRHQVIVPRKGILELARLLTEPDGNVSIVLGQHHIRATTGEFTFTSKLVDG
KFPDYERVLPKGGDKLVLGDRQALREAFSRTAILSNEKYRGIRLQLASGQLKIQANNPEQEEAEEEVGVEYNGGSLEIGF
NVSYLLDVLGVMTTEQVRLILSDSNSSALVQESDNDDSAYVVMPMRL

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=368, Percent_Identity=55.7065217391304, Blast_Score=412, Evalue=1e-116,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 40481; Mature: 40481

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHFTIQREALLKPLQLVAGVVERRQTLPVLSNVLLVVDGQQLSLTGTDLEVELVGRVQLE
CCEEECHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCEEEEECCCEEEEEEEEEEEC
EPAETGSITVPARKLMDICKSLPNDALIDIKVDEQKLVVKAGRSRFTLSTLPANDFPTVE
CCCCCCCEEECHHHHHHHHHHCCCCCEEEEEECCCEEEEECCCCEEEEEECCCCCCCCCC
EGPGSLTTSLEQSKLRRLIERTSFAMAQQDVRYYLNGMLLEVSTGVIRAVATDGHRLAMC
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCEEEEEECCCCEEEEE
SMQADIGQQDRHQVIVPRKGILELARLLTEPDGNVSIVLGQHHIRATTGEFTFTSKLVDG
EECCCCCCCCCCEEECCCHHHHHHHHHHCCCCCCEEEEEECCEEEEECCCEEEEEEECCC
KFPDYERVLPKGGDKLVLGDRQALREAFSRTAILSNEKYRGIRLQLASGQLKIQANNPEQ
CCCCHHHHCCCCCCEEEECCHHHHHHHHHHHEEECCCCCCEEEEEEECCEEEEEECCCCH
EEAEEEVGVEYNGGSLEIGFNVSYLLDVLGVMTTEQVRLILSDSNSSALVQESDNDDSAY
HHHHHHCCEEECCCEEEECCCHHHHHHHHHHCCCCEEEEEEECCCCCEEEEECCCCCCEE
VVMPMRL
EEEEECC
>Mature Secondary Structure
MHFTIQREALLKPLQLVAGVVERRQTLPVLSNVLLVVDGQQLSLTGTDLEVELVGRVQLE
CCEEECHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCEEEEECCCEEEEEEEEEEEC
EPAETGSITVPARKLMDICKSLPNDALIDIKVDEQKLVVKAGRSRFTLSTLPANDFPTVE
CCCCCCCEEECHHHHHHHHHHCCCCCEEEEEECCCEEEEECCCCEEEEEECCCCCCCCCC
EGPGSLTTSLEQSKLRRLIERTSFAMAQQDVRYYLNGMLLEVSTGVIRAVATDGHRLAMC
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCEEEEEECCCCEEEEE
SMQADIGQQDRHQVIVPRKGILELARLLTEPDGNVSIVLGQHHIRATTGEFTFTSKLVDG
EECCCCCCCCCCEEECCCHHHHHHHHHHCCCCCCEEEEEECCEEEEECCCEEEEEEECCC
KFPDYERVLPKGGDKLVLGDRQALREAFSRTAILSNEKYRGIRLQLASGQLKIQANNPEQ
CCCCHHHHCCCCCCEEEECCHHHHHHHHHHHEEECCCCCCEEEEEEECCEEEEEECCCCH
EEAEEEVGVEYNGGSLEIGFNVSYLLDVLGVMTTEQVRLILSDSNSSALVQESDNDDSAY
HHHHHHCCEEECCCEEEECCCHHHHHHHHHHCCCCEEEEEEECCCCCEEEEECCCCCCEE
VVMPMRL
EEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12534463 [H]