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The map label for this gene is irlS2 [H]

Identifier: 76819668

GI number: 76819668

Start: 1382051

End: 1383361

Strand: Direct

Name: irlS2 [H]

Synonym: BURPS1710b_A1104

Alternate gene names: 76819668

Gene position: 1382051-1383361 (Clockwise)

Preceding gene: 76819077

Following gene: 76817858

Centisome position: 43.44

GC content: 65.45

Gene sequence:

>1311_bases
ATGGAGACGACGCTCGCCGGCGTCGTCGCGCACATCGGAGAGGCGCGCAGCATCGCCGAGCTGCGCGAGAAGGGAACCAA
GTTCTACTACTCGTTCCACAGCGACGAAAAGGTCGATTTCGCGATCTACGACGCGCAGGGCACCGCATACATGCAAAGCG
GCTGGTACGTGCCGTACGGCGGCGTGCAGCAGGCCAAGCCGGGGCTCGATCCCGCCATCGTCGACGACAAGCGCAACGCG
AAGCGCTATCTCGTCGCCGACGCGCGCCTGACCGGCAATCCGGGCCTGCCGCTGCGCGTGGCGGTTCAGTACGACTTCCG
CCGCGAGCAGTCGCTGCTGCGCACGTGCGCGATCAGCATCGTCGCGATCATTCTCGTCGGCACCGCCGTCGCGGCGACCG
TCGCCTACTGGATCGCCGCTTACGCGCTCATGCCGCTACGCCGCTTCGCCGCGCAGGCCGATCAGATCTCGAGCAGCCGG
CTCGCGCAGCCGCTGCCCGAAACGGAAATGGCAGGCGAGCTCAGGGAACTCGCGGTGTCGTTCAACCGGATGCTCGTGCG
GCTGAACGCATCGTTCACGCGGCTGAAGGAGTACTCGTCCGATCTCGCGCACGACATCCGCACGCCGCTCACCAACCTGC
TCGCCGAGGCGCAGGTCGCGCTATCGCAGCGGCGCTCGGTCGACGAATATCGTCAGGTGATCGAATCGAGCGTCGAGGAA
TACCAGCGGCTCGCCAGGATGGTCGACGACATGCTGTTCCTCGCGCGCGCGGACGGCCATCAGAACAAGCTCGACATCGA
ACCGATCGACGCCTATGCGATGGCCGACCGGATCGTCCGCTACTACGAGACGGTCGCCGAGGATCGCGGCATCGGCATCG
CCGTCGAAGGCCGGGCGAGCTTCAGCGGCGATTCGATCCTCGTGCAACGGGCGCTGAGCAATCTGCTGTCCAACGCGATC
CGCCATGCGCCCGATCATTCAACGGTCGTCGTCCGTTGCGAGCAATCGGAGCAAGACGTCGTGATCGCCGTGACCGACAC
CGGCCGCGGAATCGAAGCCGTGCATCTGCCGCGGATCTTCGACCGCTTCTATCGCGTCGATCCTTCGCGATACGACTCCG
CATCCGGCACCGGGCTCGGGCTCGCGATCGTGCGCTCGATCATGACCGAACACGACGGCGATTGCTGGGTGGAAAGCATG
CCGAACGTCTTCACGACGTTCTTCCTGCGCTTTCCTCGCGAGCACGCGGCCGGCTGCGGCGATTGCCCTCCAGCGGGCCA
TGATTCGCGTTGGCATCTCGAACAGACTTGA

Upstream 100 bases:

>100_bases
GATCAAGTTCGCGCTGTCGACATCGGTGCTGCTCGCGATCAGCGGCCTGCTGCTGTACGAAACGCTCACGCGCACGTTTG
CATATTCGTCGGAGCGCGCG

Downstream 100 bases:

>100_bases
GTCATTTCGCGAATCATTTTAAATTCGAATTCGGCTTTTTCATCAAAATGACAAAAACTTCATAGCGGAGTCACGTTGAC
GGAGGGCTCGCTGTTCAAAA

Product: IrlS

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 436; Mature: 436

Protein sequence:

>436_residues
METTLAGVVAHIGEARSIAELREKGTKFYYSFHSDEKVDFAIYDAQGTAYMQSGWYVPYGGVQQAKPGLDPAIVDDKRNA
KRYLVADARLTGNPGLPLRVAVQYDFRREQSLLRTCAISIVAIILVGTAVAATVAYWIAAYALMPLRRFAAQADQISSSR
LAQPLPETEMAGELRELAVSFNRMLVRLNASFTRLKEYSSDLAHDIRTPLTNLLAEAQVALSQRRSVDEYRQVIESSVEE
YQRLARMVDDMLFLARADGHQNKLDIEPIDAYAMADRIVRYYETVAEDRGIGIAVEGRASFSGDSILVQRALSNLLSNAI
RHAPDHSTVVVRCEQSEQDVVIAVTDTGRGIEAVHLPRIFDRFYRVDPSRYDSASGTGLGLAIVRSIMTEHDGDCWVESM
PNVFTTFFLRFPREHAAGCGDCPPAGHDSRWHLEQT

Sequences:

>Translated_436_residues
METTLAGVVAHIGEARSIAELREKGTKFYYSFHSDEKVDFAIYDAQGTAYMQSGWYVPYGGVQQAKPGLDPAIVDDKRNA
KRYLVADARLTGNPGLPLRVAVQYDFRREQSLLRTCAISIVAIILVGTAVAATVAYWIAAYALMPLRRFAAQADQISSSR
LAQPLPETEMAGELRELAVSFNRMLVRLNASFTRLKEYSSDLAHDIRTPLTNLLAEAQVALSQRRSVDEYRQVIESSVEE
YQRLARMVDDMLFLARADGHQNKLDIEPIDAYAMADRIVRYYETVAEDRGIGIAVEGRASFSGDSILVQRALSNLLSNAI
RHAPDHSTVVVRCEQSEQDVVIAVTDTGRGIEAVHLPRIFDRFYRVDPSRYDSASGTGLGLAIVRSIMTEHDGDCWVESM
PNVFTTFFLRFPREHAAGCGDCPPAGHDSRWHLEQT
>Mature_436_residues
METTLAGVVAHIGEARSIAELREKGTKFYYSFHSDEKVDFAIYDAQGTAYMQSGWYVPYGGVQQAKPGLDPAIVDDKRNA
KRYLVADARLTGNPGLPLRVAVQYDFRREQSLLRTCAISIVAIILVGTAVAATVAYWIAAYALMPLRRFAAQADQISSSR
LAQPLPETEMAGELRELAVSFNRMLVRLNASFTRLKEYSSDLAHDIRTPLTNLLAEAQVALSQRRSVDEYRQVIESSVEE
YQRLARMVDDMLFLARADGHQNKLDIEPIDAYAMADRIVRYYETVAEDRGIGIAVEGRASFSGDSILVQRALSNLLSNAI
RHAPDHSTVVVRCEQSEQDVVIAVTDTGRGIEAVHLPRIFDRFYRVDPSRYDSASGTGLGLAIVRSIMTEHDGDCWVESM
PNVFTTFFLRFPREHAAGCGDCPPAGHDSRWHLEQT

Specific function: Member of the two-component regulatory system irlR/irlS. May be involved in invasion of eukaryotic cells and heavy-metal resistance. Probably activates irlR by phosphorylation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1786783, Length=316, Percent_Identity=39.2405063291139, Blast_Score=222, Evalue=4e-59,
Organism=Escherichia coli, GI1788279, Length=323, Percent_Identity=28.1733746130031, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI1790346, Length=260, Percent_Identity=33.0769230769231, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1786600, Length=227, Percent_Identity=27.7533039647577, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1787894, Length=276, Percent_Identity=29.3478260869565, Blast_Score=95, Evalue=8e-21,
Organism=Escherichia coli, GI1790861, Length=290, Percent_Identity=26.2068965517241, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI145693157, Length=221, Percent_Identity=27.1493212669683, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1788393, Length=253, Percent_Identity=24.5059288537549, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1789403, Length=299, Percent_Identity=28.7625418060201, Blast_Score=85, Evalue=8e-18,
Organism=Escherichia coli, GI1790436, Length=221, Percent_Identity=28.5067873303167, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI87081816, Length=247, Percent_Identity=25.5060728744939, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1789149, Length=230, Percent_Identity=23.9130434782609, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1787374, Length=263, Percent_Identity=25.4752851711027, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1788549, Length=222, Percent_Identity=26.1261261261261, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI1790551, Length=288, Percent_Identity=24.6527777777778, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI48994928, Length=220, Percent_Identity=25.9090909090909, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1786912, Length=224, Percent_Identity=25.8928571428571, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1788713, Length=226, Percent_Identity=20.353982300885, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR006290
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 48571; Mature: 48571

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METTLAGVVAHIGEARSIAELREKGTKFYYSFHSDEKVDFAIYDAQGTAYMQSGWYVPYG
CCCHHHHHHHHHHCHHHHHHHHHCCCEEEEEECCCCCEEEEEEECCCCEEECCCCCCCCC
GVQQAKPGLDPAIVDDKRNAKRYLVADARLTGNPGLPLRVAVQYDFRREQSLLRTCAISI
CHHHCCCCCCCCEECCCCCCCEEEEEECEECCCCCCCEEEEEEECHHHHHHHHHHHHHHH
VAIILVGTAVAATVAYWIAAYALMPLRRFAAQADQISSSRLAQPLPETEMAGELRELAVS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
FNRMLVRLNASFTRLKEYSSDLAHDIRTPLTNLLAEAQVALSQRRSVDEYRQVIESSVEE
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
YQRLARMVDDMLFLARADGHQNKLDIEPIDAYAMADRIVRYYETVAEDRGIGIAVEGRAS
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCC
FSGDSILVQRALSNLLSNAIRHAPDHSTVVVRCEQSEQDVVIAVTDTGRGIEAVHLPRIF
CCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCEEEEEECCCCCCEEEHHHHHH
DRFYRVDPSRYDSASGTGLGLAIVRSIMTEHDGDCWVESMPNVFTTFFLRFPREHAAGCG
HHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHCHHHHHHHHHHCCHHHCCCCC
DCPPAGHDSRWHLEQT
CCCCCCCCCCCCCCCC
>Mature Secondary Structure
METTLAGVVAHIGEARSIAELREKGTKFYYSFHSDEKVDFAIYDAQGTAYMQSGWYVPYG
CCCHHHHHHHHHHCHHHHHHHHHCCCEEEEEECCCCCEEEEEEECCCCEEECCCCCCCCC
GVQQAKPGLDPAIVDDKRNAKRYLVADARLTGNPGLPLRVAVQYDFRREQSLLRTCAISI
CHHHCCCCCCCCEECCCCCCCEEEEEECEECCCCCCCEEEEEEECHHHHHHHHHHHHHHH
VAIILVGTAVAATVAYWIAAYALMPLRRFAAQADQISSSRLAQPLPETEMAGELRELAVS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
FNRMLVRLNASFTRLKEYSSDLAHDIRTPLTNLLAEAQVALSQRRSVDEYRQVIESSVEE
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
YQRLARMVDDMLFLARADGHQNKLDIEPIDAYAMADRIVRYYETVAEDRGIGIAVEGRAS
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCC
FSGDSILVQRALSNLLSNAIRHAPDHSTVVVRCEQSEQDVVIAVTDTGRGIEAVHLPRIF
CCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCEEEEEECCCCCCEEEHHHHHH
DRFYRVDPSRYDSASGTGLGLAIVRSIMTEHDGDCWVESMPNVFTTFFLRFPREHAAGCG
HHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHCHHHHHHHHHHCCHHHCCCCC
DCPPAGHDSRWHLEQT
CCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9393784 [H]