Click here to switch to the map view.

The map label for this gene is rffG [C]

Identifier: 76819485

GI number: 76819485

Start: 2009563

End: 2010543

Strand: Direct

Name: rffG [C]

Synonym: BURPS1710b_A1646

Alternate gene names: 76819485

Gene position: 2009563-2010543 (Clockwise)

Preceding gene: 76819339

Following gene: 76818623

Centisome position: 63.16

GC content: 70.74

Gene sequence:

>981_bases
ATGACAGTGGCACGCAACGACGGGCGCGGCGACGCGCGGCAACGCGAGCGCGCGAAGCGCAAGGTTCTGATCACGGGCGC
GACGGGCCAGGTGGCGCGGCCCGTGGCCGAAGCGCTGGCGGCGCAGGACGAGGTGTGGGCGATCGGCCGGTTCGGCGACG
CGGGCGTCGAGCACGCGCTGCGCGCGAAGGGCGTGAAGACGTGGCGGTGGGACATGGAGCGCGACACGCTCGACAGCCTG
CCCGACGATTTCACGCACGTGCTGCACGCGGCGGTGCGCCGCGGCGAGGACGGCGATTTCGACAGGGCGATCGAGATCAA
CACGGTGGCCACGGGCCGCCTGATGACGCACTGCCGCCACGCGGAATCGTTCATCTACGTGTCGAGCGGCAGCCTGTACG
CGCGGCAGGCGCTCGACCATCCGTACGCGGAAACGGATCCGCTGAACGGCGTCGCGCACTGGCTGCCCGCCTATCCGATC
GTCAAGATCTCGTGCGAGGGCGTCGTGCGCGCGCTGTCGGCCACGCTCGGCCTGCCGACCGTGATCGCGCGCCTGAACGT
CGCGTACGGCCCGTACGGCCACGGCGGCGTGCCGGTGCTGCTGTACCGGCAGTTGCTCGCCGGCAAGCCGGTGCCGGTGC
CGCGCGAAGGCCAGAACTGGGCGTCGCTGATCCACACCGACGATCTCGTGAGGCAGGTGCCGCTGCTGTGGGAGGCGGCG
AGCTCGCCCGCGCTCGTGTTGAACTGGGGCGGCGACGATGCGGTGGGCATTCGGGATTGCCTGCAGTACGTCGCGGACCT
CACGGGCGTCGATGCGCGTTTCGAGCGCAGCGACGTGACGCGCGAGACCTATGCGTTCGACAACACGAAGCGGCGCGCGC
TGATCGGCGATTGCACGGTGCACTGGAAAGACGGCGTGCGCCGCACCATCGAAACCCACTTCCCCGGCGCGGTGAAGACG
CAGCCGGCCGCGCAGCGCTGA

Upstream 100 bases:

>100_bases
CGTCGCGACGTTCCAGGCGGTGCTGCGGGCCGAGCTCGATCGCGCGCTCGGCGGCAAATGACCGAGGTCCGGCCTCACGA
TCAACCAGGAGAGGATGCTC

Downstream 100 bases:

>100_bases
GACGCGACACAGGAGACACCGCGATGTCGAACAACATGCAGACCGTGCGCGAATCGTACGAAGCCTTCCACCGCCGGGAC
CTGCCCGGCGTGCTCGCGGC

Product: putative dehydratase

Products: dTDP-4-dehydro-6-deoxy-D-glucose; H2O [C]

Alternate protein names: Dehydratase; NAD Dependent Epimerase/Dehydratase Family Protein; Nucleotide Sugar Epimerase; Nucleoside-Diphosphate-Sugar Epimerase WcaG-Like Protein; NAD Dependent Epimerase/Dehydratase

Number of amino acids: Translated: 326; Mature: 325

Protein sequence:

>326_residues
MTVARNDGRGDARQRERAKRKVLITGATGQVARPVAEALAAQDEVWAIGRFGDAGVEHALRAKGVKTWRWDMERDTLDSL
PDDFTHVLHAAVRRGEDGDFDRAIEINTVATGRLMTHCRHAESFIYVSSGSLYARQALDHPYAETDPLNGVAHWLPAYPI
VKISCEGVVRALSATLGLPTVIARLNVAYGPYGHGGVPVLLYRQLLAGKPVPVPREGQNWASLIHTDDLVRQVPLLWEAA
SSPALVLNWGGDDAVGIRDCLQYVADLTGVDARFERSDVTRETYAFDNTKRRALIGDCTVHWKDGVRRTIETHFPGAVKT
QPAAQR

Sequences:

>Translated_326_residues
MTVARNDGRGDARQRERAKRKVLITGATGQVARPVAEALAAQDEVWAIGRFGDAGVEHALRAKGVKTWRWDMERDTLDSL
PDDFTHVLHAAVRRGEDGDFDRAIEINTVATGRLMTHCRHAESFIYVSSGSLYARQALDHPYAETDPLNGVAHWLPAYPI
VKISCEGVVRALSATLGLPTVIARLNVAYGPYGHGGVPVLLYRQLLAGKPVPVPREGQNWASLIHTDDLVRQVPLLWEAA
SSPALVLNWGGDDAVGIRDCLQYVADLTGVDARFERSDVTRETYAFDNTKRRALIGDCTVHWKDGVRRTIETHFPGAVKT
QPAAQR
>Mature_325_residues
TVARNDGRGDARQRERAKRKVLITGATGQVARPVAEALAAQDEVWAIGRFGDAGVEHALRAKGVKTWRWDMERDTLDSLP
DDFTHVLHAAVRRGEDGDFDRAIEINTVATGRLMTHCRHAESFIYVSSGSLYARQALDHPYAETDPLNGVAHWLPAYPIV
KISCEGVVRALSATLGLPTVIARLNVAYGPYGHGGVPVLLYRQLLAGKPVPVPREGQNWASLIHTDDLVRQVPLLWEAAS
SPALVLNWGGDDAVGIRDCLQYVADLTGVDARFERSDVTRETYAFDNTKRRALIGDCTVHWKDGVRRTIETHFPGAVKTQ
PAAQR

Specific function: INVOLVED IN THE SYNTHESIS OF ENTEROBACTERIAL COMMON ANTIGEN (ECA) AND REQUIRED FOR SYNTHESIS OF LIPOPOLYSACCHARIDE O-SIDE CHAINS. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 4.2.1.46 [C]

Molecular weight: Translated: 35886; Mature: 35755

Theoretical pI: Translated: 7.69; Mature: 7.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVARNDGRGDARQRERAKRKVLITGATGQVARPVAEALAAQDEVWAIGRFGDAGVEHAL
CCCCCCCCCCHHHHHHHHHCEEEEECCCCHHHHHHHHHHHCCCCEEEEECCCCHHHHHHH
RAKGVKTWRWDMERDTLDSLPDDFTHVLHAAVRRGEDGDFDRAIEINTVATGRLMTHCRH
HHCCCCEEECCCCHHHHHCCCHHHHHHHHHHHHCCCCCCCCCEEEEEEEEHHHHHHHHHH
AESFIYVSSGSLYARQALDHPYAETDPLNGVAHWLPAYPIVKISCEGVVRALSATLGLPT
CCCEEEEECCCHHHHHHHCCCCCCCCCCCHHHHHCCCCCEEEEEHHHHHHHHHHHHCHHH
VIARLNVAYGPYGHGGVPVLLYRQLLAGKPVPVPREGQNWASLIHTDDLVRQVPLLWEAA
HHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
SSPALVLNWGGDDAVGIRDCLQYVADLTGVDARFERSDVTRETYAFDNTKRRALIGDCTV
CCCEEEEECCCCCCCCHHHHHHHHHHHCCCCCHHHHHHCCHHHHHCCCCCCEEEEECEEE
HWKDGVRRTIETHFPGAVKTQPAAQR
EEHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
TVARNDGRGDARQRERAKRKVLITGATGQVARPVAEALAAQDEVWAIGRFGDAGVEHAL
CCCCCCCCCHHHHHHHHHCEEEEECCCCHHHHHHHHHHHCCCCEEEEECCCCHHHHHHH
RAKGVKTWRWDMERDTLDSLPDDFTHVLHAAVRRGEDGDFDRAIEINTVATGRLMTHCRH
HHCCCCEEECCCCHHHHHCCCHHHHHHHHHHHHCCCCCCCCCEEEEEEEEHHHHHHHHHH
AESFIYVSSGSLYARQALDHPYAETDPLNGVAHWLPAYPIVKISCEGVVRALSATLGLPT
CCCEEEEECCCHHHHHHHCCCCCCCCCCCHHHHHCCCCCEEEEEHHHHHHHHHHHHCHHH
VIARLNVAYGPYGHGGVPVLLYRQLLAGKPVPVPREGQNWASLIHTDDLVRQVPLLWEAA
HHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
SSPALVLNWGGDDAVGIRDCLQYVADLTGVDARFERSDVTRETYAFDNTKRRALIGDCTV
CCCEEEEECCCCCCCCHHHHHHHHHHHCCCCCHHHHHHCCHHHHHCCCCCCEEEEECEEE
HWKDGVRRTIETHFPGAVKTQPAAQR
EEHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.093 {dTDPglucose}} [C]

Substrates: dTDPglucose [C]

Specific reaction: dTDPglucose --> dTDP-4-dehydro-6-deoxy-D-glucose + H2O [C]

General reaction: Elimination (of H2O C-O bond cleavage [C]

Inhibitor: p-Chloromercuribenzoate; TMP [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA