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The map label for this gene is prpF [H]

Identifier: 76819477

GI number: 76819477

Start: 2121657

End: 2122847

Strand: Direct

Name: prpF [H]

Synonym: BURPS1710b_A1739

Alternate gene names: 76819477

Gene position: 2121657-2122847 (Clockwise)

Preceding gene: 76819682

Following gene: 76817952

Centisome position: 66.68

GC content: 70.78

Gene sequence:

>1191_bases
ATGGCCCACGTACCTCAAATCAAGATTCCCGCGACCTACCTGCGCGGCGGCACCAGCAAGGGCGTGTTCTTCCGGCTGCA
GGATCTGCCCGAGGCCGCGCGAACGCCGGGCGCCGCGCGCGACGCGCTGCTCTCGCGCGTGATCGGCAGCCCCGACCCGT
ACGGCAAGCAGATCGACGGCATGGGCGGCGCGAGCTCGAGCACGAGCAAGACGGTGATCGTCTCGAAGAGCGCGCGGCCC
GATCACGACGTCGACTACCTGTTCGGTCAGGTGTCGATCGACAAGCCGTTCGTCGACTGGAGCGGCAACTGCGGCAACCT
GTCGGCGGCCGTCGGCCCGTTCGCGATCGCGGGCGGCCTGATCGACCCGGCGCGCGTGCCGGAGAACGGCGTCGCGACCG
TGCGGATCTGGCAGGCGAACATCGGCAAGACGATCGTCGCGCACGTGCCGATCACGAACGGCGCGGTGCAGGAGACGGGC
GATTTCGAGCTCGACGGCGTGACGTTCCCGGCCGCCGAGGTGCAGCTCGAATTCATGGACCCGGCCGCCGACGAGGACGG
CGCGGGCGGCGCGATGTTCCCGACGGGCAACCTCGTCGACGATCTCGACGTGCCGGGCGTCGGCACGCTGAAGGCGACGA
TGATCAACGCGGGCATTCCGACGATCTTCGTCGACGCCGCGGCGATCGGCTACACCGGCACCGAGCTGCAGGACGCGATC
AATTCGGACGCGAAGGCGCTCGCGATGTTCGAGACGATCCGCGCGCACGGCGCGCTGCGCATGGGCCTCATTGCGTCGCT
CGACGAGATCGCGACGCGCCAGCACACGCCGAAGGTGGCGTTCGTCGCGAAGCCGGCGGATTACGTCGCGTCGAGCGGCA
AGCGCGTCGCGGCGGCCGACGTCGACCTGCTCGTGCGCGCGCTATCGATGGGCAAGCTGCATCACGCGATGATGGGCACG
GCGGCGGTGGCGATCGGCACCGCGGCGGCGATTCCGGGCACGCTCGTGAATCTCGCGGCGGGCGGCGGCGCGCGCCACGC
GGTGCGCTTCGGCCATCCGTCGGGCACGCTGCGCGTCGGCGCGGAGGCGAAGCAGGACGGCGGCGAGTGGGTCGTCACGA
AGGCGATCATGAGCCGCAGCGCGCGCGTGCTGATGGAGGGCTGGGTGCGCGTGCCGGGCGACGCGTTCTGA

Upstream 100 bases:

>100_bases
CGTTCGCCGCCGAGGCGAACGGGCCCGGTTCACTTGCTTCGCATGAGACCGGAGCCGGCGCCGCGGCGCTGCCTCCGATC
GATACAGGACCTCACGAACC

Downstream 100 bases:

>100_bases
CGCCGCGGGCGCGGGGCGCGGCGATCGGGCGTGCGGCGGCCTTGCCGCCGCGTTATGCCTTTTCTTCGGCCGCGCCCGGC
TCGCGCCCGTCGCCGACCCG

Product: AcnD-accessory protein PrpF

Products: NA

Alternate protein names: (R)-3-methylitaconate isomerase; 3-methylitaconate delta-isomerase [H]

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MAHVPQIKIPATYLRGGTSKGVFFRLQDLPEAARTPGAARDALLSRVIGSPDPYGKQIDGMGGASSSTSKTVIVSKSARP
DHDVDYLFGQVSIDKPFVDWSGNCGNLSAAVGPFAIAGGLIDPARVPENGVATVRIWQANIGKTIVAHVPITNGAVQETG
DFELDGVTFPAAEVQLEFMDPAADEDGAGGAMFPTGNLVDDLDVPGVGTLKATMINAGIPTIFVDAAAIGYTGTELQDAI
NSDAKALAMFETIRAHGALRMGLIASLDEIATRQHTPKVAFVAKPADYVASSGKRVAAADVDLLVRALSMGKLHHAMMGT
AAVAIGTAAAIPGTLVNLAAGGGARHAVRFGHPSGTLRVGAEAKQDGGEWVVTKAIMSRSARVLMEGWVRVPGDAF

Sequences:

>Translated_396_residues
MAHVPQIKIPATYLRGGTSKGVFFRLQDLPEAARTPGAARDALLSRVIGSPDPYGKQIDGMGGASSSTSKTVIVSKSARP
DHDVDYLFGQVSIDKPFVDWSGNCGNLSAAVGPFAIAGGLIDPARVPENGVATVRIWQANIGKTIVAHVPITNGAVQETG
DFELDGVTFPAAEVQLEFMDPAADEDGAGGAMFPTGNLVDDLDVPGVGTLKATMINAGIPTIFVDAAAIGYTGTELQDAI
NSDAKALAMFETIRAHGALRMGLIASLDEIATRQHTPKVAFVAKPADYVASSGKRVAAADVDLLVRALSMGKLHHAMMGT
AAVAIGTAAAIPGTLVNLAAGGGARHAVRFGHPSGTLRVGAEAKQDGGEWVVTKAIMSRSARVLMEGWVRVPGDAF
>Mature_395_residues
AHVPQIKIPATYLRGGTSKGVFFRLQDLPEAARTPGAARDALLSRVIGSPDPYGKQIDGMGGASSSTSKTVIVSKSARPD
HDVDYLFGQVSIDKPFVDWSGNCGNLSAAVGPFAIAGGLIDPARVPENGVATVRIWQANIGKTIVAHVPITNGAVQETGD
FELDGVTFPAAEVQLEFMDPAADEDGAGGAMFPTGNLVDDLDVPGVGTLKATMINAGIPTIFVDAAAIGYTGTELQDAIN
SDAKALAMFETIRAHGALRMGLIASLDEIATRQHTPKVAFVAKPADYVASSGKRVAAADVDLLVRALSMGKLHHAMMGTA
AVAIGTAAAIPGTLVNLAAGGGARHAVRFGHPSGTLRVGAEAKQDGGEWVVTKAIMSRSARVLMEGWVRVPGDAF

Specific function: Catalyzes the reversible isomerization of (R)-3- methylitaconate to 2,3-dimethylmaleate. Has very low isomerase activity with itaconate [H]

COG id: COG2828

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786985, Length=385, Percent_Identity=33.5064935064935, Blast_Score=172, Evalue=3e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007400 [H]

Pfam domain/function: PF04303 PrpF [H]

EC number: =5.3.3.6 [H]

Molecular weight: Translated: 40914; Mature: 40783

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAHVPQIKIPATYLRGGTSKGVFFRLQDLPEAARTPGAARDALLSRVIGSPDPYGKQIDG
CCCCCCEECCHHHHCCCCCCCEEEEECCCCHHHHCCCHHHHHHHHHHHCCCCCCCCCCCC
MGGASSSTSKTVIVSKSARPDHDVDYLFGQVSIDKPFVDWSGNCGNLSAAVGPFAIAGGL
CCCCCCCCCEEEEEECCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCHHHCCHHHHCCC
IDPARVPENGVATVRIWQANIGKTIVAHVPITNGAVQETGDFELDGVTFPAAEVQLEFMD
CCCCCCCCCCCEEEEEEECCCCCEEEEEECCCCCCCCCCCCEEECCEECCHHEEEEEEEC
PAADEDGAGGAMFPTGNLVDDLDVPGVGTLKATMINAGIPTIFVDAAAIGYTGTELQDAI
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCEEEEEHHHCCCCCHHHHHHH
NSDAKALAMFETIRAHGALRMGLIASLDEIATRQHTPKVAFVAKPADYVASSGKRVAAAD
CCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHCCCCEEEHHH
VDLLVRALSMGKLHHAMMGTAAVAIGTAAAIPGTLVNLAAGGGARHAVRFGHPSGTLRVG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEEECCCCCCCEEEECCCCCCEEEC
AEAKQDGGEWVVTKAIMSRSARVLMEGWVRVPGDAF
CCCCCCCCCEEEEHHHHHHHHHHHHHCCEECCCCCC
>Mature Secondary Structure 
AHVPQIKIPATYLRGGTSKGVFFRLQDLPEAARTPGAARDALLSRVIGSPDPYGKQIDG
CCCCCEECCHHHHCCCCCCCEEEEECCCCHHHHCCCHHHHHHHHHHHCCCCCCCCCCCC
MGGASSSTSKTVIVSKSARPDHDVDYLFGQVSIDKPFVDWSGNCGNLSAAVGPFAIAGGL
CCCCCCCCCEEEEEECCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCHHHCCHHHHCCC
IDPARVPENGVATVRIWQANIGKTIVAHVPITNGAVQETGDFELDGVTFPAAEVQLEFMD
CCCCCCCCCCCEEEEEEECCCCCEEEEEECCCCCCCCCCCCEEECCEECCHHEEEEEEEC
PAADEDGAGGAMFPTGNLVDDLDVPGVGTLKATMINAGIPTIFVDAAAIGYTGTELQDAI
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCEEEEEHHHCCCCCHHHHHHH
NSDAKALAMFETIRAHGALRMGLIASLDEIATRQHTPKVAFVAKPADYVASSGKRVAAAD
CCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHCCCCEEEHHH
VDLLVRALSMGKLHHAMMGTAAVAIGTAAAIPGTLVNLAAGGGARHAVRFGHPSGTLRVG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEEECCCCCCCEEEECCCCCCEEEC
AEAKQDGGEWVVTKAIMSRSARVLMEGWVRVPGDAF
CCCCCCCCCEEEEHHHHHHHHHHHHHCCEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA