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The map label for this gene is safB [H]

Identifier: 76819464

GI number: 76819464

Start: 1446916

End: 1448769

Strand: Direct

Name: safB [H]

Synonym: BURPS1710b_A1161

Alternate gene names: 76819464

Gene position: 1446916-1448769 (Clockwise)

Preceding gene: 76818264

Following gene: 76819726

Centisome position: 45.48

GC content: 69.69

Gene sequence:

>1854_bases
ATGACACATCCGCGCGAAACGACGGCCGCCGACACGTTCGCCGACTTCGTCGAACTCACGCGATATCGCGCCGCGCATCA
GCCCGATCTGCCGGTCTACACGTTCGTCACCGGCGGCGATCGCGACGAACGACCTCTCACATGCGCGCAGTTGGACAGGC
GCGCAAGCGCCGTGGCGGCAGCGCTATCGGAAATCGCCCGGCCCGGCGAGCGCGTGCTGCTGCTGTTTGCACCCGGCATC
GACTATATCGCCGCGCTGTTCGGCTGCATGTACGCGGGCGTCGTGGCCGTGCCCGCGTATCCGGTCGAGCCCGCGCAGCC
CGAGCGCACGCTCCGGCGGCTGCTCGGCATCGTCGCGGATTGCGCGCCCGCCGCGGTGCTGTCGACGGCGGCCGTGCGCG
ACGGCATGAACCGCGTCGAAACCGGTTCGCCCGCGCTGCGCGGCCTGCGCTGGATCGAGATCGACGCACTCTCGCCCGGC
GACGATGCCGCGCACGGCGCGCCGCGCGCGGCCGACCCGCGTGTGCCCGTCTACCTGCAATACACGTCGGGTTCGACCGG
CGCGCCGAAGGGCGTGATGATCAGCCACCGGAACCTGCTGCACAACTCCGCGCTGATCGCGCGCCGCTTCGAGCACGGCG
CGAGCAGCCGCGGCGTGATCTGGCTGCCGCCGTATCACGACATGGGTCTGATCGGCGGCATCCTGCAGCCGCTGTACGTC
GGCTTTCCTGTGACGCTGATGTCGCACGTCGATTTTCTCAAGCACCCGCTGCGCTGGCTGCGCGCGATCGGCGAGCGCCG
CGCGACGACGAGCGGCGGGCCGAACTTCGCGTATCAGATGCTCGCGACGATGCGCATCGCCGACGCCGATTTCGACAAGC
TCGACCTGCGCTCGTGGGACGTCGCGTTCGTCGGCGCGGAGCCGATCCGGGCCGCCACGCTGCACGCGTTCGCGCAGCGC
TTCGCGCGCTGCGGCTTCGATGCGCGCGCGTTCTATCCGTGCTACGGCCTCGCCGAACACACGCTGTTCATGACGGGCGG
ACTGAAATCGCAGCCGCCCGTCGTCGCGAACGAGCCAAGCGACGCGCGGCTGCCGCGCGCATCCGACCACGCCGACGCCC
CCGGCCAGGCCGACCCGGCCGGCGACGGGCGGCAAGCAGGTGCGCGCGCCGCCGTCGGATGCGGCGACGCGGCCAGCGAC
AGTTTGGTGCTGATCGTCGATCCCGACACGCGCGTTCCGTGCGATGATGGCCGGGTCGGCGAAATCTGGGCGCAAGGGCC
GAGCGTCGCGCTCGGTTACTGGAACAATCGCGCGCTCAGCGAGCAGACCTTCGAGGCCGAGCTGCCCGGCTACGCGGGGC
GATTCCTGCGCACCGGCGATTACGGCTATCGGTCGGGCTCCGAAGTGTTCGTCACCGGGCGGCTGAAGGACATGATGCTG
ATTCGCGGCGCGAATCATTATCCGCACGACGTCGAGGCGACGATCGAGGCGCTCGACGCCGAGCTGTTCCGCCCCGGCGG
CTGCGCGGTGTTCGCGCTCGATACCGGCGCGGCGCCGCAAGTGACCGTCGTGCGCGAGTTGCGGGCGCGCTATTTGAAGG
CATTCGGCGACGGCGGCCAAGAAGCCGGCCACACGCCCGACGCGCTGTTCGGCAGGCTGCGTCGGGCGATCAACCTGCAT
CACGGCATTGCGGTACACCATATCGTCTTCACGTCGCCTTCTGCGATACCGAAGACGACGAGCGGAAAGGTCCAGCGGCA
CGCCTGTCGCGAACTGTTTCTCAACGACACGCTGCCGGTGGTCACCCAGTGGCGCGCGCCGTGCGGCGCGCCGAACGACA
TCCGGAACATCTGA

Upstream 100 bases:

>100_bases
ATGCGCATCGCGCGAGCGATGCGAACGACGCCGGCGCGCACGAGGATGCTTCGGCCGCCGCCCCGGTCTCGGCCGGCCAA
CCAGCCACGGAGCCGACACG

Downstream 100 bases:

>100_bases
CGAGGAACACGGCATGCAAGAGAACCTGGACGCGGACTACTTCAACCGTTTCGGCGCCGACTATCTGCCGGGCCTGCTCG
GCGTCGTCGTCAAGCAGGTC

Product: saframycin Mx1 synthetase B

Products: AMP; diphosphate +an acyl-CoA [C]

Alternate protein names: Acyl-CoA synthetase [H]

Number of amino acids: Translated: 617; Mature: 616

Protein sequence:

>617_residues
MTHPRETTAADTFADFVELTRYRAAHQPDLPVYTFVTGGDRDERPLTCAQLDRRASAVAAALSEIARPGERVLLLFAPGI
DYIAALFGCMYAGVVAVPAYPVEPAQPERTLRRLLGIVADCAPAAVLSTAAVRDGMNRVETGSPALRGLRWIEIDALSPG
DDAAHGAPRAADPRVPVYLQYTSGSTGAPKGVMISHRNLLHNSALIARRFEHGASSRGVIWLPPYHDMGLIGGILQPLYV
GFPVTLMSHVDFLKHPLRWLRAIGERRATTSGGPNFAYQMLATMRIADADFDKLDLRSWDVAFVGAEPIRAATLHAFAQR
FARCGFDARAFYPCYGLAEHTLFMTGGLKSQPPVVANEPSDARLPRASDHADAPGQADPAGDGRQAGARAAVGCGDAASD
SLVLIVDPDTRVPCDDGRVGEIWAQGPSVALGYWNNRALSEQTFEAELPGYAGRFLRTGDYGYRSGSEVFVTGRLKDMML
IRGANHYPHDVEATIEALDAELFRPGGCAVFALDTGAAPQVTVVRELRARYLKAFGDGGQEAGHTPDALFGRLRRAINLH
HGIAVHHIVFTSPSAIPKTTSGKVQRHACRELFLNDTLPVVTQWRAPCGAPNDIRNI

Sequences:

>Translated_617_residues
MTHPRETTAADTFADFVELTRYRAAHQPDLPVYTFVTGGDRDERPLTCAQLDRRASAVAAALSEIARPGERVLLLFAPGI
DYIAALFGCMYAGVVAVPAYPVEPAQPERTLRRLLGIVADCAPAAVLSTAAVRDGMNRVETGSPALRGLRWIEIDALSPG
DDAAHGAPRAADPRVPVYLQYTSGSTGAPKGVMISHRNLLHNSALIARRFEHGASSRGVIWLPPYHDMGLIGGILQPLYV
GFPVTLMSHVDFLKHPLRWLRAIGERRATTSGGPNFAYQMLATMRIADADFDKLDLRSWDVAFVGAEPIRAATLHAFAQR
FARCGFDARAFYPCYGLAEHTLFMTGGLKSQPPVVANEPSDARLPRASDHADAPGQADPAGDGRQAGARAAVGCGDAASD
SLVLIVDPDTRVPCDDGRVGEIWAQGPSVALGYWNNRALSEQTFEAELPGYAGRFLRTGDYGYRSGSEVFVTGRLKDMML
IRGANHYPHDVEATIEALDAELFRPGGCAVFALDTGAAPQVTVVRELRARYLKAFGDGGQEAGHTPDALFGRLRRAINLH
HGIAVHHIVFTSPSAIPKTTSGKVQRHACRELFLNDTLPVVTQWRAPCGAPNDIRNI
>Mature_616_residues
THPRETTAADTFADFVELTRYRAAHQPDLPVYTFVTGGDRDERPLTCAQLDRRASAVAAALSEIARPGERVLLLFAPGID
YIAALFGCMYAGVVAVPAYPVEPAQPERTLRRLLGIVADCAPAAVLSTAAVRDGMNRVETGSPALRGLRWIEIDALSPGD
DAAHGAPRAADPRVPVYLQYTSGSTGAPKGVMISHRNLLHNSALIARRFEHGASSRGVIWLPPYHDMGLIGGILQPLYVG
FPVTLMSHVDFLKHPLRWLRAIGERRATTSGGPNFAYQMLATMRIADADFDKLDLRSWDVAFVGAEPIRAATLHAFAQRF
ARCGFDARAFYPCYGLAEHTLFMTGGLKSQPPVVANEPSDARLPRASDHADAPGQADPAGDGRQAGARAAVGCGDAASDS
LVLIVDPDTRVPCDDGRVGEIWAQGPSVALGYWNNRALSEQTFEAELPGYAGRFLRTGDYGYRSGSEVFVTGRLKDMMLI
RGANHYPHDVEATIEALDAELFRPGGCAVFALDTGAAPQVTVVRELRARYLKAFGDGGQEAGHTPDALFGRLRRAINLHH
GIAVHHIVFTSPSAIPKTTSGKVQRHACRELFLNDTLPVVTQWRAPCGAPNDIRNI

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI225735629, Length=626, Percent_Identity=26.038338658147, Blast_Score=133, Evalue=6e-31,
Organism=Homo sapiens, GI45827692, Length=626, Percent_Identity=26.038338658147, Blast_Score=132, Evalue=8e-31,
Organism=Homo sapiens, GI44888818, Length=617, Percent_Identity=24.9594813614263, Blast_Score=129, Evalue=1e-29,
Organism=Homo sapiens, GI55749758, Length=525, Percent_Identity=24, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI225735625, Length=599, Percent_Identity=22.3706176961603, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI156151445, Length=589, Percent_Identity=22.4108658743633, Blast_Score=86, Evalue=1e-16,
Organism=Homo sapiens, GI45827694, Length=529, Percent_Identity=22.6843100189036, Blast_Score=84, Evalue=4e-16,
Organism=Homo sapiens, GI45827696, Length=503, Percent_Identity=22.4652087475149, Blast_Score=82, Evalue=2e-15,
Organism=Homo sapiens, GI225735627, Length=503, Percent_Identity=22.4652087475149, Blast_Score=82, Evalue=2e-15,
Organism=Homo sapiens, GI45827698, Length=462, Percent_Identity=21.8614718614719, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI1788107, Length=619, Percent_Identity=23.5864297253635, Blast_Score=94, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI193202679, Length=632, Percent_Identity=24.3670886075949, Blast_Score=125, Evalue=8e-29,
Organism=Drosophila melanogaster, GI24654656, Length=472, Percent_Identity=25.8474576271186, Blast_Score=117, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3 [C]

Molecular weight: Translated: 66621; Mature: 66490

Theoretical pI: Translated: 7.18; Mature: 7.18

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHPRETTAADTFADFVELTRYRAAHQPDLPVYTFVTGGDRDERPLTCAQLDRRASAVAA
CCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHH
ALSEIARPGERVLLLFAPGIDYIAALFGCMYAGVVAVPAYPVEPAQPERTLRRLLGIVAD
HHHHHHCCCCEEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
CAPAAVLSTAAVRDGMNRVETGSPALRGLRWIEIDALSPGDDAAHGAPRAADPRVPVYLQ
HHHHHHHHHHHHHHCHHHHCCCCHHHHCEEEEEEECCCCCCCCCCCCCCCCCCCCCEEEE
YTSGSTGAPKGVMISHRNLLHNSALIARRFEHGASSRGVIWLPPYHDMGLIGGILQPLYV
ECCCCCCCCCCEEEECHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHHHHH
GFPVTLMSHVDFLKHPLRWLRAIGERRATTSGGPNFAYQMLATMRIADADFDKLDLRSWD
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCC
VAFVGAEPIRAATLHAFAQRFARCGFDARAFYPCYGLAEHTLFMTGGLKSQPPVVANEPS
EEEECCCCHHHHHHHHHHHHHHHCCCCCCEECCCCCHHHCEEEEECCCCCCCCEECCCCC
DARLPRASDHADAPGQADPAGDGRQAGARAAVGCGDAASDSLVLIVDPDTRVPCDDGRVG
CCCCCCCCCCCCCCCCCCCCCCCHHCCCCCEECCCCCCCCCEEEEECCCCCCCCCCCCCC
EIWAQGPSVALGYWNNRALSEQTFEAELPGYAGRFLRTGDYGYRSGSEVFVTGRLKDMML
EEEECCCCEEEEECCCCCCCCHHHHHCCCCCCCCCEECCCCCCCCCCEEEEEECHHEEHE
IRGANHYPHDVEATIEALDAELFRPGGCAVFALDTGAAPQVTVVRELRARYLKAFGDGGQ
EECCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCC
EAGHTPDALFGRLRRAINLHHGIAVHHIVFTSPSAIPKTTSGKVQRHACRELFLNDTLPV
CCCCCHHHHHHHHHHHHHHHCCEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCH
VTQWRAPCGAPNDIRNI
HCCCCCCCCCCHHHCCC
>Mature Secondary Structure 
THPRETTAADTFADFVELTRYRAAHQPDLPVYTFVTGGDRDERPLTCAQLDRRASAVAA
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHH
ALSEIARPGERVLLLFAPGIDYIAALFGCMYAGVVAVPAYPVEPAQPERTLRRLLGIVAD
HHHHHHCCCCEEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
CAPAAVLSTAAVRDGMNRVETGSPALRGLRWIEIDALSPGDDAAHGAPRAADPRVPVYLQ
HHHHHHHHHHHHHHCHHHHCCCCHHHHCEEEEEEECCCCCCCCCCCCCCCCCCCCCEEEE
YTSGSTGAPKGVMISHRNLLHNSALIARRFEHGASSRGVIWLPPYHDMGLIGGILQPLYV
ECCCCCCCCCCEEEECHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHHHHH
GFPVTLMSHVDFLKHPLRWLRAIGERRATTSGGPNFAYQMLATMRIADADFDKLDLRSWD
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCC
VAFVGAEPIRAATLHAFAQRFARCGFDARAFYPCYGLAEHTLFMTGGLKSQPPVVANEPS
EEEECCCCHHHHHHHHHHHHHHHCCCCCCEECCCCCHHHCEEEEECCCCCCCCEECCCCC
DARLPRASDHADAPGQADPAGDGRQAGARAAVGCGDAASDSLVLIVDPDTRVPCDDGRVG
CCCCCCCCCCCCCCCCCCCCCCCHHCCCCCEECCCCCCCCCEEEEECCCCCCCCCCCCCC
EIWAQGPSVALGYWNNRALSEQTFEAELPGYAGRFLRTGDYGYRSGSEVFVTGRLKDMML
EEEECCCCEEEEECCCCCCCCHHHHHCCCCCCCCCEECCCCCCCCCCEEEEEECHHEEHE
IRGANHYPHDVEATIEALDAELFRPGGCAVFALDTGAAPQVTVVRELRARYLKAFGDGGQ
EECCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCC
EAGHTPDALFGRLRRAINLHHGIAVHHIVFTSPSAIPKTTSGKVQRHACRELFLNDTLPV
CCCCCHHHHHHHHHHHHHHHCCEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCH
VTQWRAPCGAPNDIRNI
HCCCCCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0013 {undecanoate}} 0.0022 {pentadecanoate}} 0.053 {CoA}} 0.035 {CoA}} 0.034 {CoA}} 0.0034 {tridecanoate}} 0.002 {laurate}} 0.0019 {laurate}} 0.0

Substrates: ATP; a long-chain carboxylic acid; CoA [C]

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate +an acyl-CoA [C]

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]