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The map label for this gene is bopE [H]

Identifier: 76819430

GI number: 76819430

Start: 757715

End: 758500

Strand: Direct

Name: bopE [H]

Synonym: BURPS1710b_A0564

Alternate gene names: 76819430

Gene position: 757715-758500 (Clockwise)

Preceding gene: 76819587

Following gene: 76819691

Centisome position: 23.81

GC content: 68.96

Gene sequence:

>786_bases
ATGACTTACAACCCGAGAATCGGCGGATTCACGCACGTCAAGCAGGCGTCGTTCGACGTTCACGTCAAGCGCGGCGAAGC
GCAGCCGCGCACGTCGTTCGCACAACAGATCAAGCGCATCTTCAGCAAGATCGGCGAAACGCTCGGGCAACTGTTCCGCC
ACCGCGCGCCGGACAGCGCGCCCGGCCGGGTCAGGCTGCAGGGCGTGCGCTACGTCGGCAGCTATCGGCCCACGGGCGAC
GCGAAACAGGCGATCCGGCATTTCGTCGACGAAGCGGTCAAGCAGGTCGCCCACGCGCGTACCCCGGAGATCCGGCAGGA
TGCCGAATTCGGCCGGCAAGTCTACGAAGCGACGCTCTGCGCGATCTTCAGCGAAGCGAAGGACCGGTTCTGCATGGACC
CGGCCACGCGCGCGGGCAACGTGCGGCCGGCGTTCATCGAGGCGCTCGGCGACGCGGCGCGTGCGACGGGGCTGCCCGGC
GCCGACAAGCAAGGCGTGTTCACGCCGAGCGGCGCCGGCACGAATCCGCTCTACACCGAGATCCGGCTGCGCGCCGATAC
GCTGATGGGCGCCGAGCTGGCCGCGCGCCCGGAGTATCGCGAGCTTCAGCCGTATGCGCGGCAGCAGGCGATCGATCTCG
TCGCGAACGCGTTGCCGGCCGAGCGTTCGAACACGCTCGTCGAGTTCCGGCAGACGGTGCAGACGCTCGAAGCCACATAC
CGCCGCGCGGCACAAGACGCGTCGCGCGACGAGAAGGGCGCGACGAACGCGGCGGACGGCGCGTGA

Upstream 100 bases:

>100_bases
AATCGGTCCGGACAGCCGCGCCCGCCACCGCGACGCACGACACACGACACACGACGCGGCAGGCGCGCCGGACCTCCTTC
CCTTCAACCGAGAGCAAGAC

Downstream 100 bases:

>100_bases
CGCGGTAGCGTGGTCCGGACCGTCCGGCGCCTCCGCCCGGATGGTTCGGATCGCTGAATTCGTGGGGTTCTCGGGGGTTC
TCGGGGGTTCTCGGGGGTTC

Product: type III secretion target BopE

Products: NA

Alternate protein names: Effector protein BopE [H]

Number of amino acids: Translated: 261; Mature: 260

Protein sequence:

>261_residues
MTYNPRIGGFTHVKQASFDVHVKRGEAQPRTSFAQQIKRIFSKIGETLGQLFRHRAPDSAPGRVRLQGVRYVGSYRPTGD
AKQAIRHFVDEAVKQVAHARTPEIRQDAEFGRQVYEATLCAIFSEAKDRFCMDPATRAGNVRPAFIEALGDAARATGLPG
ADKQGVFTPSGAGTNPLYTEIRLRADTLMGAELAARPEYRELQPYARQQAIDLVANALPAERSNTLVEFRQTVQTLEATY
RRAAQDASRDEKGATNAADGA

Sequences:

>Translated_261_residues
MTYNPRIGGFTHVKQASFDVHVKRGEAQPRTSFAQQIKRIFSKIGETLGQLFRHRAPDSAPGRVRLQGVRYVGSYRPTGD
AKQAIRHFVDEAVKQVAHARTPEIRQDAEFGRQVYEATLCAIFSEAKDRFCMDPATRAGNVRPAFIEALGDAARATGLPG
ADKQGVFTPSGAGTNPLYTEIRLRADTLMGAELAARPEYRELQPYARQQAIDLVANALPAERSNTLVEFRQTVQTLEATY
RRAAQDASRDEKGATNAADGA
>Mature_260_residues
TYNPRIGGFTHVKQASFDVHVKRGEAQPRTSFAQQIKRIFSKIGETLGQLFRHRAPDSAPGRVRLQGVRYVGSYRPTGDA
KQAIRHFVDEAVKQVAHARTPEIRQDAEFGRQVYEATLCAIFSEAKDRFCMDPATRAGNVRPAFIEALGDAARATGLPGA
DKQGVFTPSGAGTNPLYTEIRLRADTLMGAELAARPEYRELQPYARQQAIDLVANALPAERSNTLVEFRQTVQTLEATYR
RAAQDASRDEKGATNAADGA

Specific function: Activator for both CDC42 and RAC1 by directly interacting with these Rho GTPases and acting as a guanine nucleotide exchange factor (GEF). This activation results in actin cytoskeleton rearrangements and stimulates membrane ruffling, thus promoting bacter

COG id: NA

COG function: NA

Gene ontology:

Cell location: Secreted. Note=Secreted via the bsa type III secretion system (By similarity) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GEF (guanine exchange factor) sopE family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005414
- InterPro:   IPR016019 [H]

Pfam domain/function: PF07487 SopE_GEF [H]

EC number: NA

Molecular weight: Translated: 28706; Mature: 28574

Theoretical pI: Translated: 9.89; Mature: 9.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTYNPRIGGFTHVKQASFDVHVKRGEAQPRTSFAQQIKRIFSKIGETLGQLFRHRAPDSA
CCCCCCCCCHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PGRVRLQGVRYVGSYRPTGDAKQAIRHFVDEAVKQVAHARTPEIRQDAEFGRQVYEATLC
CCCEEEEEEHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
AIFSEAKDRFCMDPATRAGNVRPAFIEALGDAARATGLPGADKQGVFTPSGAGTNPLYTE
HHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHH
IRLRADTLMGAELAARPEYRELQPYARQQAIDLVANALPAERSNTLVEFRQTVQTLEATY
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
RRAAQDASRDEKGATNAADGA
HHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
TYNPRIGGFTHVKQASFDVHVKRGEAQPRTSFAQQIKRIFSKIGETLGQLFRHRAPDSA
CCCCCCCCHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PGRVRLQGVRYVGSYRPTGDAKQAIRHFVDEAVKQVAHARTPEIRQDAEFGRQVYEATLC
CCCEEEEEEHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
AIFSEAKDRFCMDPATRAGNVRPAFIEALGDAARATGLPGADKQGVFTPSGAGTNPLYTE
HHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHH
IRLRADTLMGAELAARPEYRELQPYARQQAIDLVANALPAERSNTLVEFRQTVQTLEATY
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
RRAAQDASRDEKGATNAADGA
HHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA