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The map label for this gene is araF [H]

Identifier: 76819353

GI number: 76819353

Start: 323769

End: 325139

Strand: Direct

Name: araF [H]

Synonym: BURPS1710b_A0214

Alternate gene names: 76819353

Gene position: 323769-325139 (Clockwise)

Preceding gene: 76817761

Following gene: 76818619

Centisome position: 10.18

GC content: 67.98

Gene sequence:

>1371_bases
GTGCTTTTCCCGCGACGCCCCGCCGCCTTCGACCTTCGCCCTTCGACCTTCGACCTTCGACCTTCGACCTTCGACCTTCG
ACCTCAGACTTCAGACCTTCGACGTCCGACGTCCGACGTCGGACCTTCGACCCCGACGCCCGACATCGGACATCGGACAT
CGGACATCGGACATCGGACATCCGGCATCCAAACTCGACATCCGCCCCACCCCTCGCCTCCCCACCGCCCGATCCCATTC
TTTAGGAACCCCTCGCAATATTTGGAAGCCCGCCGACGCGCCTCCTACAATCAGCCGCACGACATGCATGCCGTTGCGCG
GCTCGCAAGGCCGCATCCATATCGAAAACGGAGCACGAAGGAGACGCTCATGGGATTGCGCTGGCCCCAAGCCGCCCTCG
TCTGCGCGAGCCTCGCCGCCGGTTTGTCGGCGGCGGCGCCCGCGCATGCGCAAGGCGCGGCCCCGGTGAAGATCGGCTTC
GTCGTCAAGCAGCCCGACGACCCGTGGTTTCAGGACGAATGGCGCTTCGCCGAGCAGGCGGCGAAGGACAAGCACTTCAC
GCTCGTGAAGATCGCCGCGCCGAGCGGCGAGAAGGTGTCGACCGCGCTCGACAGCCTCGCCGCGCAAAAGGCGCAGGGTG
TGATCATCTGCGCGCCCGACGTGAAGCTCGGCCCCGGCATCGCCGCGAAGGCGAGGCGCTACGGGATGAAGCTGATGTCG
GTCGACGATCAGCTCGTCGACGGGCGCGGCGCGCCGCTCGCCGACGTGCCGCACATGGGCATTTCCGCATACCGGATCGG
CCGGCAGGTCGGCGACGCGATCGCCGCCGAGGCGAAGCGGCGCGGCTGGAATCCGGCCGAGGTCGGCGTGCTGCGCCTCG
CGTACGACCAGTTGCCGACCGCGCGCGAGCGCACGACGGGCGCGGTCGATGCGCTGAAGGCCGCGGGCTTCGCGGCGGCG
AACGTCGTCGACGCGCCGGAGATGACGGCCGATACCGAAGGCGCGTTCAACGCCGCGAACATCGCGTTCACCAAGCATCG
GAACTTCAAGCACTGGGTGGCGTTCGGATCGAATGACGACACGACGGTCGGCGCGGTGCGCGCGGGCGAGGGGCGCGGCA
TCGGGGCGGACGACATGATCGCGGTCGGCATCAACGGCAGCCAGGTCGCGCTGAACGAATTCGCGAAACCGAAGCCGACG
GGCTTTTTCGGCTCGATCCTGCTGAATCCGCGGCTGCACGGCTACGACACGTCGGTCAACATGTACGACTGGATCACGCA
GAACCGCGCGCCGCCGCCGGTCGTGCTGACGTCCGGCACGCTGATCACGCGCGCGAACGAAAAGACGGCGCGCGCGCAGC
TCGGGCTGTGA

Upstream 100 bases:

>100_bases
CCGCCCGACGCACCCATCGCGGCGGACAAGCCGGCCGGCGCCGGCGCGAAGCCGCAGGCGAAGGCAAAAACGTGACGGCG
GCGGGCACGGGCGGCGAATC

Downstream 100 bases:

>100_bases
AGCGCGCCGACGCCGCACGGCTGGCGGCGCCGGCGGCGTTGGGTTTCGAACCGGAGGAATGCGCATGACGACATCACGCA
CCACGCTGATCACGGGCGCC

Product: L-arabinose ABC transporter periplasmic L-arabinose-binding protein

Products: ADP; phosphate; arabinose [Cytoplasm] [C]

Alternate protein names: ABP [H]

Number of amino acids: Translated: 456; Mature: 456

Protein sequence:

>456_residues
MLFPRRPAAFDLRPSTFDLRPSTFDLRPQTSDLRRPTSDVGPSTPTPDIGHRTSDIGHRTSGIQTRHPPHPSPPHRPIPF
FRNPSQYLEARRRASYNQPHDMHAVARLARPHPYRKRSTKETLMGLRWPQAALVCASLAAGLSAAAPAHAQGAAPVKIGF
VVKQPDDPWFQDEWRFAEQAAKDKHFTLVKIAAPSGEKVSTALDSLAAQKAQGVIICAPDVKLGPGIAAKARRYGMKLMS
VDDQLVDGRGAPLADVPHMGISAYRIGRQVGDAIAAEAKRRGWNPAEVGVLRLAYDQLPTARERTTGAVDALKAAGFAAA
NVVDAPEMTADTEGAFNAANIAFTKHRNFKHWVAFGSNDDTTVGAVRAGEGRGIGADDMIAVGINGSQVALNEFAKPKPT
GFFGSILLNPRLHGYDTSVNMYDWITQNRAPPPVVLTSGTLITRANEKTARAQLGL

Sequences:

>Translated_456_residues
MLFPRRPAAFDLRPSTFDLRPSTFDLRPQTSDLRRPTSDVGPSTPTPDIGHRTSDIGHRTSGIQTRHPPHPSPPHRPIPF
FRNPSQYLEARRRASYNQPHDMHAVARLARPHPYRKRSTKETLMGLRWPQAALVCASLAAGLSAAAPAHAQGAAPVKIGF
VVKQPDDPWFQDEWRFAEQAAKDKHFTLVKIAAPSGEKVSTALDSLAAQKAQGVIICAPDVKLGPGIAAKARRYGMKLMS
VDDQLVDGRGAPLADVPHMGISAYRIGRQVGDAIAAEAKRRGWNPAEVGVLRLAYDQLPTARERTTGAVDALKAAGFAAA
NVVDAPEMTADTEGAFNAANIAFTKHRNFKHWVAFGSNDDTTVGAVRAGEGRGIGADDMIAVGINGSQVALNEFAKPKPT
GFFGSILLNPRLHGYDTSVNMYDWITQNRAPPPVVLTSGTLITRANEKTARAQLGL
>Mature_456_residues
MLFPRRPAAFDLRPSTFDLRPSTFDLRPQTSDLRRPTSDVGPSTPTPDIGHRTSDIGHRTSGIQTRHPPHPSPPHRPIPF
FRNPSQYLEARRRASYNQPHDMHAVARLARPHPYRKRSTKETLMGLRWPQAALVCASLAAGLSAAAPAHAQGAAPVKIGF
VVKQPDDPWFQDEWRFAEQAAKDKHFTLVKIAAPSGEKVSTALDSLAAQKAQGVIICAPDVKLGPGIAAKARRYGMKLMS
VDDQLVDGRGAPLADVPHMGISAYRIGRQVGDAIAAEAKRRGWNPAEVGVLRLAYDQLPTARERTTGAVDALKAAGFAAA
NVVDAPEMTADTEGAFNAANIAFTKHRNFKHWVAFGSNDDTTVGAVRAGEGRGIGADDMIAVGINGSQVALNEFAKPKPT
GFFGSILLNPRLHGYDTSVNMYDWITQNRAPPPVVLTSGTLITRANEKTARAQLGL

Specific function: Involved in the high-affinity L-arabinose membrane transport system. Binds with high affinity to arabinose, but can also bind D-galactose (approximately 2-fold reduction) and D- fucose (approximately 40-fold reduction) [H]

COG id: COG1879

COG function: function code G; ABC-type sugar transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 2 family [H]

Homologues:

Organism=Escherichia coli, GI1788211, Length=326, Percent_Identity=45.7055214723926, Blast_Score=295, Evalue=5e-81,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001761 [H]

Pfam domain/function: PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 49260; Mature: 49260

Theoretical pI: Translated: 10.47; Mature: 10.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLFPRRPAAFDLRPSTFDLRPSTFDLRPQTSDLRRPTSDVGPSTPTPDIGHRTSDIGHRT
CCCCCCCCCCCCCCCCCCCCCCEEECCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCC
SGIQTRHPPHPSPPHRPIPFFRNPSQYLEARRRASYNQPHDMHAVARLARPHPYRKRSTK
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCHHCCHH
ETLMGLRWPQAALVCASLAAGLSAAAPAHAQGAAPVKIGFVVKQPDDPWFQDEWRFAEQA
HHHHHCCCCHHHHHHHHHHHCHHHCCCCCCCCCCCEEEEEEEECCCCCCCCHHHHHHHHH
AKDKHFTLVKIAAPSGEKVSTALDSLAAQKAQGVIICAPDVKLGPGIAAKARRYGMKLMS
CCCCCEEEEEEECCCCCHHHHHHHHHHHHHHCEEEEECCCCCCCCCHHHHHHHHCEEEEE
VDDQLVDGRGAPLADVPHMGISAYRIGRQVGDAIAAEAKRRGWNPAEVGVLRLAYDQLPT
CCHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHEEEEHHHHCCC
ARERTTGAVDALKAAGFAAANVVDAPEMTADTEGAFNAANIAFTKHRNFKHWVAFGSNDD
HHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCCCCCCCEEEEEECCCCEEEEEECCCCC
TTVGAVRAGEGRGIGADDMIAVGINGSQVALNEFAKPKPTGFFGSILLNPRLHGYDTSVN
CEEEEEECCCCCCCCCCCEEEEECCCCCHHHHHCCCCCCCCEEEEEEECCEECCCCCCCC
MYDWITQNRAPPPVVLTSGTLITRANEKTARAQLGL
HHHHHHCCCCCCCEEEECCEEEEECCCHHHHHHCCC
>Mature Secondary Structure
MLFPRRPAAFDLRPSTFDLRPSTFDLRPQTSDLRRPTSDVGPSTPTPDIGHRTSDIGHRT
CCCCCCCCCCCCCCCCCCCCCCEEECCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCC
SGIQTRHPPHPSPPHRPIPFFRNPSQYLEARRRASYNQPHDMHAVARLARPHPYRKRSTK
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCHHCCHH
ETLMGLRWPQAALVCASLAAGLSAAAPAHAQGAAPVKIGFVVKQPDDPWFQDEWRFAEQA
HHHHHCCCCHHHHHHHHHHHCHHHCCCCCCCCCCCEEEEEEEECCCCCCCCHHHHHHHHH
AKDKHFTLVKIAAPSGEKVSTALDSLAAQKAQGVIICAPDVKLGPGIAAKARRYGMKLMS
CCCCCEEEEEEECCCCCHHHHHHHHHHHHHHCEEEEECCCCCCCCCHHHHHHHHCEEEEE
VDDQLVDGRGAPLADVPHMGISAYRIGRQVGDAIAAEAKRRGWNPAEVGVLRLAYDQLPT
CCHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHEEEEHHHHCCC
ARERTTGAVDALKAAGFAAANVVDAPEMTADTEGAFNAANIAFTKHRNFKHWVAFGSNDD
HHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCCCCCCCEEEEEECCCCEEEEEECCCCC
TTVGAVRAGEGRGIGADDMIAVGINGSQVALNEFAKPKPTGFFGSILLNPRLHGYDTSVN
CEEEEEECCCCCCCCCCCEEEEECCCCCHHHHHCCCCCCCCEEEEEEECCEECCCCCCCC
MYDWITQNRAPPPVVLTSGTLITRANEKTARAQLGL
HHHHHHCCCCCCCEEEECCEEEEECCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; arabinose [Periplasm]; H2O [C]

Specific reaction: ATP + arabinose [Periplasm] + H2O = ADP + phosphate + arabinose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2445996; 9097040; 9278503; 6885805; 326784; 9298646; 7031057; 326785; 2204627 [H]