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The map label for this gene is proP [H]

Identifier: 76819337

GI number: 76819337

Start: 2199971

End: 2201308

Strand: Direct

Name: proP [H]

Synonym: BURPS1710b_A1809

Alternate gene names: 76819337

Gene position: 2199971-2201308 (Clockwise)

Preceding gene: 76818428

Following gene: 76817401

Centisome position: 69.14

GC content: 70.03

Gene sequence:

>1338_bases
ATGCACGAAGACAACCTGACGATGCCCGCCGACGGTGCGGGCGTCGCCCCGGCCGACGCCGTATCGGCGGCGGGCTTGCG
CACCGCGCGCGGCGGTGCGATCGCGGCCGCGGTGATCGGCAACTGGCTCGAATTCTTCGATTTCACCGTCTACGGTTTTT
TCGCGGTGATCATCGGCAAGCTGTATTTCCCGTCGCACGACGCGACGACGTCGCTGCTGCTGTCGGTCGCGACGTTCGCC
GCGGGCTTCTTCACGCGTCCGCTCGGCAGCATCGTGCTCGGCGTCTACGCGGACCGCCGCGGCCGCAAGGCGGCGCTGAA
CCTGACGATCCTGCTGATGGCGCTCGGCACCGGCATGATCGCGCTCGCGCCGACCTACGCGCAGATCGGCGTGCTCGCGC
CGGTGATCGTCGTGTGCGCGCGGCTGATGCAGGGCTTTTCGCAAGGCGGAGAGTTCGGCGCGGCGACGTCGACGCTCGTC
GAGCACGGCGGCGCCGCGCGCCGGGGCTTTCGCGCGAGCTGGCAGCTCGCGACGCAGGGCGGCGCGGCGCTGATGGGCTC
GGGGTTCGCGGCGCTGCTGTCGAACACGCTCGCGAAGGACGCGCTGGAAAGCTGGGGCTGGCGCGTGCCGTTCGCGCTCG
GTGTGCTGATCGCGCCCGTCGGCATGTATCTGCGCCGCCGCCTGGCCGACGATGCGCCGGGCGCCGGCCACCACGGCATC
GACGGCGGCGTGCTGCGCGAGCTGTTCGCGCGGCACGGCCGCACGGTGCTGCTGCTGACGCTCACGGTGATGGGCGGCAC
CGTATCGACGTACATCCTGACGTTCTACATGCCGACCTACGCGATCCATACGCTCGGGCTGCCGATGAAGCTGTCGATGT
TCGTCGGCGTCGCGTCCGGCTGCGTGATGCTCGTCACCTGCCCGTTGTTCGGCTGGCTGTCCGACCGGATCGGCAGCCGG
CGCCTGCCGATCTTCGTCGGGCGCGGCGTGCTCGTCGTGCTGCTGTTTCCGGCGTTCGTGCTGATGAACCGCTATCCGTC
GCTCGCGGTCGTGATGCCGCTCACCGGGCTGATGCTGCTGTTCTATTCGATGGGCTCGGCGTCCGAGTTCGCGCTGATGT
GCGAATCGTTTCCGCGCCGCGTGCGCGCGACGGGCATCTCGATCGCCTATGCGCTCGCGGTGACGCTGTTCGGCGGCACC
GCACAGCTCGTCGCGACGTGGCTCGTGCGCGTGACGGGCAGCACGCTCGCGCCGGCCGCTTACGTGGCCGTGTGCGTGAT
CGTGTCGCTCGTCGCGGTCGGGATGCTGCGCGAGACGGCGACGGAAACGGCCGACTGA

Upstream 100 bases:

>100_bases
CCGGTTCATGCCGCCCGACGCCAATCGAAGCCCCGCGAGCCGCGCAGGCGTCGGCCGCGGTTGCGCTTCCGGGTGACATA
TTCGACGAGGAGACAACGAC

Downstream 100 bases:

>100_bases
GCGCGTGGTGGCGCGGCGGCGCGGTGCGCGAGCGAATCGCGGCGCGGCGCCGCCGGTGTCGGCGGCGGGCCGCGGCGGGG
GAAATGGAAAGGACGGCTGA

Product: major facilitator family transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Citrate carrier protein; Citrate transporter; Citrate utilization determinant; Citrate utilization protein A [H]

Number of amino acids: Translated: 445; Mature: 445

Protein sequence:

>445_residues
MHEDNLTMPADGAGVAPADAVSAAGLRTARGGAIAAAVIGNWLEFFDFTVYGFFAVIIGKLYFPSHDATTSLLLSVATFA
AGFFTRPLGSIVLGVYADRRGRKAALNLTILLMALGTGMIALAPTYAQIGVLAPVIVVCARLMQGFSQGGEFGAATSTLV
EHGGAARRGFRASWQLATQGGAALMGSGFAALLSNTLAKDALESWGWRVPFALGVLIAPVGMYLRRRLADDAPGAGHHGI
DGGVLRELFARHGRTVLLLTLTVMGGTVSTYILTFYMPTYAIHTLGLPMKLSMFVGVASGCVMLVTCPLFGWLSDRIGSR
RLPIFVGRGVLVVLLFPAFVLMNRYPSLAVVMPLTGLMLLFYSMGSASEFALMCESFPRRVRATGISIAYALAVTLFGGT
AQLVATWLVRVTGSTLAPAAYVAVCVIVSLVAVGMLRETATETAD

Sequences:

>Translated_445_residues
MHEDNLTMPADGAGVAPADAVSAAGLRTARGGAIAAAVIGNWLEFFDFTVYGFFAVIIGKLYFPSHDATTSLLLSVATFA
AGFFTRPLGSIVLGVYADRRGRKAALNLTILLMALGTGMIALAPTYAQIGVLAPVIVVCARLMQGFSQGGEFGAATSTLV
EHGGAARRGFRASWQLATQGGAALMGSGFAALLSNTLAKDALESWGWRVPFALGVLIAPVGMYLRRRLADDAPGAGHHGI
DGGVLRELFARHGRTVLLLTLTVMGGTVSTYILTFYMPTYAIHTLGLPMKLSMFVGVASGCVMLVTCPLFGWLSDRIGSR
RLPIFVGRGVLVVLLFPAFVLMNRYPSLAVVMPLTGLMLLFYSMGSASEFALMCESFPRRVRATGISIAYALAVTLFGGT
AQLVATWLVRVTGSTLAPAAYVAVCVIVSLVAVGMLRETATETAD
>Mature_445_residues
MHEDNLTMPADGAGVAPADAVSAAGLRTARGGAIAAAVIGNWLEFFDFTVYGFFAVIIGKLYFPSHDATTSLLLSVATFA
AGFFTRPLGSIVLGVYADRRGRKAALNLTILLMALGTGMIALAPTYAQIGVLAPVIVVCARLMQGFSQGGEFGAATSTLV
EHGGAARRGFRASWQLATQGGAALMGSGFAALLSNTLAKDALESWGWRVPFALGVLIAPVGMYLRRRLADDAPGAGHHGI
DGGVLRELFARHGRTVLLLTLTVMGGTVSTYILTFYMPTYAIHTLGLPMKLSMFVGVASGCVMLVTCPLFGWLSDRIGSR
RLPIFVGRGVLVVLLFPAFVLMNRYPSLAVVMPLTGLMLLFYSMGSASEFALMCESFPRRVRATGISIAYALAVTLFGGT
AQLVATWLVRVTGSTLAPAAYVAVCVIVSLVAVGMLRETATETAD

Specific function: Uptake of citrate across the boundary membrane with the concomitant transport of protons into the cell (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=428, Percent_Identity=32.7102803738318, Blast_Score=220, Evalue=1e-58,
Organism=Escherichia coli, GI1788942, Length=406, Percent_Identity=34.2364532019704, Blast_Score=202, Evalue=4e-53,
Organism=Escherichia coli, GI1788292, Length=419, Percent_Identity=28.6396181384248, Blast_Score=168, Evalue=7e-43,
Organism=Escherichia coli, GI1789941, Length=429, Percent_Identity=32.8671328671329, Blast_Score=147, Evalue=1e-36,
Organism=Escherichia coli, GI87082404, Length=374, Percent_Identity=24.331550802139, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI87082231, Length=216, Percent_Identity=25, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 46791; Mature: 46791

Theoretical pI: Translated: 9.65; Mature: 9.65

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHEDNLTMPADGAGVAPADAVSAAGLRTARGGAIAAAVIGNWLEFFDFTVYGFFAVIIGK
CCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LYFPSHDATTSLLLSVATFAAGFFTRPLGSIVLGVYADRRGRKAALNLTILLMALGTGMI
HHCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHH
ALAPTYAQIGVLAPVIVVCARLMQGFSQGGEFGAATSTLVEHGGAARRGFRASWQLATQG
EECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCHHCCCCCCEEEECCC
GAALMGSGFAALLSNTLAKDALESWGWRVPFALGVLIAPVGMYLRRRLADDAPGAGHHGI
CCHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
DGGVLRELFARHGRTVLLLTLTVMGGTVSTYILTFYMPTYAIHTLGLPMKLSMFVGVASG
CHHHHHHHHHHCCCEEEEEEHHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
CVMLVTCPLFGWLSDRIGSRRLPIFVGRGVLVVLLFPAFVLMNRYPSLAVVMPLTGLMLL
HHHHHHHHHHHHHHHHCCCCCCCEEECCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
FYSMGSASEFALMCESFPRRVRATGISIAYALAVTLFGGTAQLVATWLVRVTGSTLAPAA
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHH
YVAVCVIVSLVAVGMLRETATETAD
HHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MHEDNLTMPADGAGVAPADAVSAAGLRTARGGAIAAAVIGNWLEFFDFTVYGFFAVIIGK
CCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LYFPSHDATTSLLLSVATFAAGFFTRPLGSIVLGVYADRRGRKAALNLTILLMALGTGMI
HHCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHH
ALAPTYAQIGVLAPVIVVCARLMQGFSQGGEFGAATSTLVEHGGAARRGFRASWQLATQG
EECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCHHCCCCCCEEEECCC
GAALMGSGFAALLSNTLAKDALESWGWRVPFALGVLIAPVGMYLRRRLADDAPGAGHHGI
CCHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
DGGVLRELFARHGRTVLLLTLTVMGGTVSTYILTFYMPTYAIHTLGLPMKLSMFVGVASG
CHHHHHHHHHHCCCEEEEEEHHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
CVMLVTCPLFGWLSDRIGSRRLPIFVGRGVLVVLLFPAFVLMNRYPSLAVVMPLTGLMLL
HHHHHHHHHHHHHHHHCCCCCCCEEECCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
FYSMGSASEFALMCESFPRRVRATGISIAYALAVTLFGGTAQLVATWLVRVTGSTLAPAA
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHH
YVAVCVIVSLVAVGMLRETATETAD
HHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]