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The map label for this gene is pilT [H]

Identifier: 76819140

GI number: 76819140

Start: 858878

End: 859909

Strand: Direct

Name: pilT [H]

Synonym: BURPS1710b_A0658

Alternate gene names: 76819140

Gene position: 858878-859909 (Clockwise)

Preceding gene: 76818429

Following gene: 76818691

Centisome position: 26.99

GC content: 66.18

Gene sequence:

>1032_bases
ATGTTTACCGACATCCACATCGAACAGGACCGCCCCGTCACGATCAAGACGCCGCGCGGCTGGGTCGAGACGAGCGACGA
ACCGGTGCTCCTCGACGACATGCGCCCTCTGCTCGATGCGATCGACGAGCACTGGGAGAGCGCGCTGCGCCAGGGCACGC
TCGACCGCCCGTTCGTCCTCACGCGCTGCCGGCTGCGCTGCCACGTGTATCGCGCGGGCGGCGGGCGCAAGATCGTGATC
TCGATGCGGCGGCTGCCGCTCAGGCCGCTGCCGCTCGAGGAGCTCGGCCTGCCCGTCTACGTGCGCTCGATGCTCGACAA
CACGAAGGGCATCATCCTCGTCACCGGCCCGACGGGCTCCGGCAAGACGACGACGATCGCTTCGCTGCTCGAGCACGTCA
ACGCGACGCGCAACACGCATATCGTCACGATCGAGGAACCGATCGAATACTATCTGGAGCGCCGCCAGTCGATCATCTCG
CAGCGCGAAGTGCCGACCGACACGCCGAATTTCCCGCACGGGCTGCGCGAGGCGCTGCGGCAGAAACCGGACGTCGTGAT
GGTGGGCGAGGTGCGCGACGCGCAGACGGCCGACACGCTGCTGCAGGCGGGCGAATCCGGGCACCTCGTGCTCGCGACGA
TGCACACGGGCAGCGCGGTCAGCGCGCTCAACAAGCTGCTGTCGTTCTTTCCGGTCGAGCAGCGCGACCGGCACGCGGTC
GCGCTCGCCGAATCGCTGATCGGCATCGTCTGCCAGAGCCTCGTGCCGAGCGCGGACGGCGAAAGCCTCGTGCTCGCGAG
CGAGTTGCTGTTCAACAACAACAACCAGGTCGCAAAGCTGATCGCGGACCCGACCAAGCTGCACCTCGTCAACGAGTTCC
TGCGCCGCCGGGAGGACAACATGTCGCGCTCGCTGAACGACGACCTGGCGGCGCTCGCGGCCCGCAACCGGATCACGACG
AAGGATGCGTTACGGGCCACTTACAATCGGCTCGAGCTGCATGAAATGATGCAGTCGATTGTTAATCGATAG

Upstream 100 bases:

>100_bases
TCCATTCATTGGAATCGCCACCAAGATGAACCAGCATGCCGCCGCCGCCCCGCTGCGCGATCTCGCGGGCGAAGTGCTCG
GCCTGATCGACCTGAAGCAG

Downstream 100 bases:

>100_bases
CGACCGCGCGAAGCGGGCCCGGCGTTTTTATACATCGATCTAAAATCGAAAAAAAATCATATAAACAAGCGGCGCCCCCG
CGTTAATCGACTGGACGCGT

Product: twitching motility protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 343; Mature: 343

Protein sequence:

>343_residues
MFTDIHIEQDRPVTIKTPRGWVETSDEPVLLDDMRPLLDAIDEHWESALRQGTLDRPFVLTRCRLRCHVYRAGGGRKIVI
SMRRLPLRPLPLEELGLPVYVRSMLDNTKGIILVTGPTGSGKTTTIASLLEHVNATRNTHIVTIEEPIEYYLERRQSIIS
QREVPTDTPNFPHGLREALRQKPDVVMVGEVRDAQTADTLLQAGESGHLVLATMHTGSAVSALNKLLSFFPVEQRDRHAV
ALAESLIGIVCQSLVPSADGESLVLASELLFNNNNQVAKLIADPTKLHLVNEFLRRREDNMSRSLNDDLAALAARNRITT
KDALRATYNRLELHEMMQSIVNR

Sequences:

>Translated_343_residues
MFTDIHIEQDRPVTIKTPRGWVETSDEPVLLDDMRPLLDAIDEHWESALRQGTLDRPFVLTRCRLRCHVYRAGGGRKIVI
SMRRLPLRPLPLEELGLPVYVRSMLDNTKGIILVTGPTGSGKTTTIASLLEHVNATRNTHIVTIEEPIEYYLERRQSIIS
QREVPTDTPNFPHGLREALRQKPDVVMVGEVRDAQTADTLLQAGESGHLVLATMHTGSAVSALNKLLSFFPVEQRDRHAV
ALAESLIGIVCQSLVPSADGESLVLASELLFNNNNQVAKLIADPTKLHLVNEFLRRREDNMSRSLNDDLAALAARNRITT
KDALRATYNRLELHEMMQSIVNR
>Mature_343_residues
MFTDIHIEQDRPVTIKTPRGWVETSDEPVLLDDMRPLLDAIDEHWESALRQGTLDRPFVLTRCRLRCHVYRAGGGRKIVI
SMRRLPLRPLPLEELGLPVYVRSMLDNTKGIILVTGPTGSGKTTTIASLLEHVNATRNTHIVTIEEPIEYYLERRQSIIS
QREVPTDTPNFPHGLREALRQKPDVVMVGEVRDAQTADTLLQAGESGHLVLATMHTGSAVSALNKLLSFFPVEQRDRHAV
ALAESLIGIVCQSLVPSADGESLVLASELLFNNNNQVAKLIADPTKLHLVNEFLRRREDNMSRSLNDDLAALAARNRITT
KDALRATYNRLELHEMMQSIVNR

Specific function: May be involved in pilus retraction [H]

COG id: COG2805

COG function: function code NU; Tfp pilus assembly protein, pilus retraction ATPase PilT

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=275, Percent_Identity=37.4545454545455, Blast_Score=156, Evalue=1e-39,
Organism=Escherichia coli, GI1789723, Length=173, Percent_Identity=45.6647398843931, Blast_Score=121, Evalue=7e-29,
Organism=Escherichia coli, GI1786296, Length=158, Percent_Identity=37.9746835443038, Blast_Score=101, Evalue=6e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 38493; Mature: 38493

Theoretical pI: Translated: 6.92; Mature: 6.92

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFTDIHIEQDRPVTIKTPRGWVETSDEPVLLDDMRPLLDAIDEHWESALRQGTLDRPFVL
CCCEEEECCCCCEEEECCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
TRCRLRCHVYRAGGGRKIVISMRRLPLRPLPLEELGLPVYVRSMLDNTKGIILVTGPTGS
HHHHHEEEEEECCCCCEEEEEECCCCCCCCCHHHCCCHHHHHHHHCCCCCEEEEECCCCC
GKTTTIASLLEHVNATRNTHIVTIEEPIEYYLERRQSIISQREVPTDTPNFPHGLREALR
CCHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
QKPDVVMVGEVRDAQTADTLLQAGESGHLVLATMHTGSAVSALNKLLSFFPVEQRDRHAV
CCCCEEEEECCCCHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCCCCHHHH
ALAESLIGIVCQSLVPSADGESLVLASELLFNNNNQVAKLIADPTKLHLVNEFLRRREDN
HHHHHHHHHHHHHHCCCCCCCCEEEEHHHHHCCCCCEEHHHCCCHHHHHHHHHHHHHHHH
MSRSLNDDLAALAARNRITTKDALRATYNRLELHEMMQSIVNR
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MFTDIHIEQDRPVTIKTPRGWVETSDEPVLLDDMRPLLDAIDEHWESALRQGTLDRPFVL
CCCEEEECCCCCEEEECCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
TRCRLRCHVYRAGGGRKIVISMRRLPLRPLPLEELGLPVYVRSMLDNTKGIILVTGPTGS
HHHHHEEEEEECCCCCEEEEEECCCCCCCCCHHHCCCHHHHHHHHCCCCCEEEEECCCCC
GKTTTIASLLEHVNATRNTHIVTIEEPIEYYLERRQSIISQREVPTDTPNFPHGLREALR
CCHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
QKPDVVMVGEVRDAQTADTLLQAGESGHLVLATMHTGSAVSALNKLLSFFPVEQRDRHAV
CCCCEEEEECCCCHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCCCCHHHH
ALAESLIGIVCQSLVPSADGESLVLASELLFNNNNQVAKLIADPTKLHLVNEFLRRREDN
HHHHHHHHHHHHHHCCCCCCCCEEEEHHHHHCCCCCEEHHHCCCHHHHHHHHHHHHHHHH
MSRSLNDDLAALAARNRITTKDALRATYNRLELHEMMQSIVNR
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8100347 [H]